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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 于昌平(Chang-Ping Yu) | |
| dc.contributor.author | Yu-Chun Chen | en |
| dc.contributor.author | 陳育囷 | zh_TW |
| dc.date.accessioned | 2021-06-17T07:08:16Z | - |
| dc.date.available | 2024-07-31 | |
| dc.date.copyright | 2019-07-31 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-07-23 | |
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Isolation of the exoelectrogenic bacterium Ochrobactrum anthropi YZ-1 by using a U-tube microbial fuel cell. Appl. Environ. Microbiol., 74(10), 3130-3137. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72856 | - |
| dc.description.abstract | 微生物燃料電池為一種再生能源，電池中具有電化學活性之微生物，能氧化廢水中的營養物質，達到處理污染物同時又產生電能之效果，因而此技術逐漸受到關注。本研究施加恆電位(200 mV、0 mV、−200 mV、−400 mV)於陽極進行電化學活性菌之馴養，待系統穩定後進行次世代定序與電化學活性(極化曲線、功率密度曲線、循環伏安法與電化學阻抗分析)的分析。分析完畢後，進行單一菌株分離之篩選，將馴養之混菌分離成為單一純菌，並將其置於微流道系統中進行單一菌株產電能力之評估。
結果顯示，施加−200 mV恆電位馴養之生物陽極具有較佳表現，該系統產生最大功率密度187.3 mW m-2，並於電化學阻抗分析中觀測出電極極化阻抗由9206降至105.8 ohms、總內阻為337 ohms，並由循環伏安法觀測出明顯之氧化還原波峰。 本研究所純化出的單一菌落普遍具有生物修復性，其中五株菌株Acinetobacter brisouii、Arcobacter lacus、Chryseobacterium cucumeris、Pseudomonas citronellolis、Pseudomonas delhiensis為尚未被評估產電能力之菌株，於本研究所建立之快篩系統觀測出具相當產電潛力。最後期許未來能將此系統加以精進並廣泛應用，以利後人更加了解單一微生物之電化學機制。 | zh_TW |
| dc.description.abstract | Microbial fuel cell (MFC) is one kind of green energy harvesting techniques. Although many exoelectrogens as essential anode-respiring bacteria have been proved to be able to produce electricity and treat pollutants simultaneously, there are still many potential exoelectrogens unclear and warrant the need for further research.
In this study, mixed-culture exoelectrogens were steadily cultivated under constant potential in the dual-chamber microbial fuel cells with microbial community analyses and electrochemical performance of biofilms being evaluated by utilizing 16S rRNA gene high throughput sequencing and power density curve, polarization curve, electrochemical impedance spectroscopy and cyclic voltammetry. After isolating diverse pure bacterial strains from the anode biofilms, we established a fast-screening system using the microfluidic laminar flow MFC (MLFMFC). By inoculating the isolated strains in the anode of MLFMFC and measuring its open circuit and closed circuit voltages, we can rapidly and efficiently identify the electroactive bacteria among these isolates. Results showed the bioanode domesticated under a constant potential of −200 mV had the better performance with maximum power density of 187.3 mW m-2. Acinetobacter brisouii, Arcobacter lacus, Chryseobacterium cucumeris, Pseudomonas citronellolis, and Pseudomonas delhiensis were isolated and first proved to be capable of producing electricity in this study by our fast-screening systems. In conclusion, this fast-screening system was successfully established and verified and it is expected to be widely utilized in the future to better isolate effective exoelectrogens. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T07:08:16Z (GMT). No. of bitstreams: 1 ntu-108-R06541205-1.pdf: 4732675 bytes, checksum: 48564cb57bba6fbd56f568a2516f1fbf (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 論文口試委員審定書 I
致謝 III 中文摘要 V Abstract VI 圖目錄 X 表目錄 XII 第一章 緒論 1 1.1 研究背景 1 1.2 研究動機與目的 2 1.3 研究流程 3 第二章 文獻回顧 5 2.1 微生物燃料電池發展與原理 5 2.1.1 生物電化學系統沿革 5 2.1.2 微生物燃料電池原理 6 2.1.3 微生物燃料電池之生物修復應用 9 2.2 雙槽式微生物燃料電池之陽極菌叢 10 2.2.1 產電菌株與電子傳遞機制探討 10 2.2.2 陽極電化學活性生物膜之富集與馴養 16 2.2.3 常見陽極菌株 17 2.3 微型微生物燃料電池 21 2.3.1 微型微生物燃料電池發展與應用 21 2.3.2 微流道微生物燃料電池 22 2.4 電化學方法於產電成效之應用 24 2.4.1 循環伏安法 24 2.4.2 交流阻抗法 24 第三章 材料與方法 25 3.1 實驗藥品與設備 25 3.1.1 實驗藥品 25 3.1.2 實驗設備 27 3.2 雙槽式微生物燃料電池生電化系統組態 28 3.2.1 雙槽式微生物燃料電池 28 3.2.1.1 雙槽式微生物燃料電池構型 28 3.2.1.2 電極前處理 29 3.2.1.3 質子交換膜前處理 30 3.2.1.4 雙槽式微生物燃料電池運行 30 3.2.2 微生物實驗 32 3.2.2.1 接種源及馴養 32 3.2.2.2 篩選菌落培養基選定 33 3.2.2.3 微生物DNA萃取 35 3.2.2.4 聚合酶連鎖反應 36 3.2.2.5 電泳膠片 37 3.2.2.6 16S rRNA 定序及次世代定序 37 3.2.2.7 螢光顯微鏡 39 3.3 微型微生物燃料電池生電化系統組態 40 3.3.1 微流道微生物燃料電池構型 40 3.3.2 微流道微生物燃料電池運行—產電菌快篩試驗 40 3.4 電化學分析 42 3.4.1 功率密度與極化曲線 42 3.4.2 循環伏安法 44 3.4.3 電化學阻抗譜 46 3.4.4 掃描式電子顯微鏡 48 第四章 結果與討論 49 4.1 雙槽式生物陽極之表現 49 4.1.1 生物陽極菌株結果 49 4.1.1.1 菌群結構 49 4.1.1.2 菌株純化 52 4.1.2 電化學分析 64 4.1.2.1 不同電位馴養生物陽極之成效 64 4.1.2.2 功率密度與極化曲線 67 4.1.2.3 循環伏安法 70 4.1.2.4 電化學阻抗譜 72 4.1.2.5 掃描式電子顯微鏡 75 4.2單一菌株於微流道槽體之表現 77 4.2.1 第一階段產電效能試驗與螢光顯微鏡驗證 77 4.2.2 單一菌株之產電效能評估 81 4.2.5 單一菌株之掃描式電子顯微鏡 86 第五章 結論與建議 89 5.1 結論 89 5.2 建議 90 第六章 參考文獻 91 | |
| dc.language.iso | zh-TW | |
| dc.subject | 微流道微生物燃料電池 | zh_TW |
| dc.subject | 快速篩選產電菌 | zh_TW |
| dc.subject | 微生物燃料電池 | zh_TW |
| dc.subject | 生物修復 | zh_TW |
| dc.subject | 恆電位 | zh_TW |
| dc.subject | 生物陽極 | zh_TW |
| dc.subject | microchannel microbial fuel cell | en |
| dc.subject | rapid screening of electrogenic bacteria | en |
| dc.subject | microbial fuel cell | en |
| dc.subject | constant potential | en |
| dc.subject | bioanode | en |
| dc.subject | bioremediation | en |
| dc.title | 定電位馴養電活性菌應用於微流道微生物燃料電池快篩系統 | zh_TW |
| dc.title | Selecting Exoelectrogens Based on Constant Anode Potential: Application of Fast-screening in Microfluidic Laminar Flow Microbial Fuel Cell System | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 郭獻文(Hsion?Wen Kuo),林居慶(Chu-Ching Lin) | |
| dc.subject.keyword | 微生物燃料電池,恆電位,生物陽極,生物修復,微流道微生物燃料電池,快速篩選產電菌, | zh_TW |
| dc.subject.keyword | microbial fuel cell,constant potential,bioanode,bioremediation,microchannel microbial fuel cell,rapid screening of electrogenic bacteria, | en |
| dc.relation.page | 106 | |
| dc.identifier.doi | 10.6342/NTU201901839 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2019-07-24 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 環境工程學研究所 | zh_TW |
| 顯示於系所單位： | 環境工程學研究所 | |
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