Please use this identifier to cite or link to this item:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72710
Full metadata record
???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
---|---|---|
dc.contributor.advisor | 楊爵因(Jiue-In Yang) | |
dc.contributor.author | Yu-Po Lin | en |
dc.contributor.author | 林鈺博 | zh_TW |
dc.date.accessioned | 2021-06-17T07:04:11Z | - |
dc.date.available | 2019-07-31 | |
dc.date.copyright | 2019-07-31 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-07-29 | |
dc.identifier.citation | 行政院農業委員會農業試驗所 (2013a) 土壤電導度測定方法. TARI S101.1B.
行政院農業委員會農業試驗所 (2013b) 土壤酸鹼值(pH值)測定方法-電極法. TARI S501.1B. 行政院農業委員會農糧署(統計室) (2017) 臺閩地區農產品生產量值-草莓. 余冠毅 (2018) 水稻白尖病與徒長病之快速檢測技術建立及應用. 國立臺灣大學植物病理與微生物研究所學位論文:1-66. 吳信郁, 蔡東纂 & 林奕耀 (2002) 臺灣作物根腐線蟲之鑑定及生物學研究. 植物病理學會刊, 11:123-136. 呂嘉彬 (2009) 摘除老葉, 走莖與花對臺灣冬季草莓生長發育與生產之影響. 國立臺灣大學園藝學研究所學位論文:1-102. 國立臺灣大學生物技術研究中心 植物組織培養與基因轉殖. 生物技術方法, 3. 陳冠霖, 張碧芳 & 黃振文 (2017) 台灣草莓萎凋病菌之生理生化特性分析. 植物醫學, 59:13-22. 陳殿義, 倪蕙芳, 蔡東纂 & 顏志恒 (2011) 台灣地區根腐線蟲 Pratylenchus coffeae (Nematoda: Pratylenchidae) 之鑑定. 植物病理學會刊, 20:26-38. 陳殿義, 倪蕙芳, 顏志恒, 吳文希 & 蔡東纂 (2009a) 台灣地區草莓和茶園根腐線蟲 Pratylenchus penetrans 和 P. loosi (Nematoda: Pratylenchidae) 之鑑定. 植物病理學會刊, 18 (4):247-262. 陳殿義, 倪蕙芳, 顏志恒 & 蔡東纂 (2009b) 台灣地區根腐線蟲新記錄種 Pratylenchus zeae (Nematoda: Tylenchoidea, Pratylenchidae) 之鑑定. 植物病理學會刊, 18:111-118. 戴肇鋒, 李祈益 & 蘇秋竹 (2018) 藥毒所病蟲害診斷中心果樹案件剖析. 臺灣農藥科學:37-52. 顏志恆, 李明達, 陳殿義, 林俊義 & 蔡東纂 (1998) 土壤植物寄生性線蟲分離方法之優劣比較. 植物保護學會會刊, 40:153-162. Al-Banna, L., Ploeg, A., Williamson, V. & Kaloshian, I. (2004) Discrimination of six Pratylenchus species using PCR and species-specific primers. Journal of Nematology, 36:142. Allen, M.W. & Jensen, H. J. (1951) Pratylenchus vulnus, new species (Nematoda Pratylenchinae), a parasite of trees and vines in California. Proceedings of the Helminthological Society of Washington 18:47-50. Amiri, S., Subbotin, S. A. & Moens, M. (2002) Identification of the beet cyst nematode Heterodera schachtii by PCR. European Journal of Plant Pathology, 108:497-506. Ann, P. J., Tsai, J. N., Hsu, Z. H., Yang, C. W., and Lin, C. P (2012) Preliminary report of fungal wilt diseases of strawberryin Taiwan. Plant Pathology Bulletin, 21:148-149. Arroyo, F., Llergo, Y., Aguado, A. & Romero, F. (2009) First report of Fusarium wilt caused by Fusarium oxysporum on strawberry in Spain. Plant Disease, 93 (3):323-323. Atkinson, G. F. (1892) Some diseases of cotton. Agricultural Experiment Station of the Agricultural and Mechanical College. Bae, C., Szalanski, A. & Robbins, R. (2008) Molecular analysis of the lance nematode, Hoplolaimus spp., using the first internal transcribed spacer and the D1-D3 expansion segments of 28S Ribosomal DNA. Journal of Nematology, 40:201-209. Baidoo, R., Yan, G., Nagachandrabose, S. & Skantar, A. M. (2017) Developing a real-time PCR qssay for direct identification and quantification of Pratylenchus penetrans in soil. Plant Disease, 101:1432-1441. Bernard, G. C., Egnin, M. & Bonsi, C. (2017) The impact of plant-parasitic nematodes on agriculture and methods of control. Nematology-Concepts, Diagnosis and Control, 121. Brentu, C., Speijer, R., Green, K., Hemeng, B., De Waele, D. & Coyne, D. (2004) Micro-plot evaluation of the yield reduction potential of Pratylenchus coffeae, Helicotylenchus multicinctus and Meloidogyne javanica on plantain cv. Apantu-pa (Musa spp., AAB-group) in Ghana. Nematology, 6:455-462. Brimblecombe, P., Beenk, J. & Mattison, C. (2014) Developing heritage climatology for collection preservation in changing sub-tropical climates through a trapping survey in Hong Kong. Studies in Conservation, 59:S9-S12. Burns, N. C. (1971) Soil pH effects on nematode populations associated with soybeans. Journal of Nematology, 3:238. Buzo, T., McKenry, M. & Hasey, J. (2004)Interaction of Juglans species with Pratylenchus vulnus and Meloidogyne incognita. In, V International Walnut Symposium 705: 417-423. Kim, C. H., Seo, H. D., Cho, W. D., & Kim, S. B. (1982) Studies on varietal resistance and chemical control to the wilt of strawberry caused by Fusarium oxysporum. Korean Journal of Applied Entomology, 21(2):61-67. Castillo, P., Mora-Rodríguez, M. P., Navas-Cortés, J. A. & Jiménez-Díaz, R. M. (1998) Interactions of Pratylenchus thornei and Fusarium oxysporum f. sp. ciceris on chickpea. Phytopathology, 88:828-836. Castillo, P. & Vovlas, N. (2007) Pratylenchus (Nematoda: Pratylenchidae): diagnosis, biology, pathogenicity and management, Brill. Chałańska, A., Łabanowski, G. & Sas, D. (2016) Root-lesion nematodes (Pratylenchus spp.) in ornamental plant nurseries–influence of soil texture, acidity, salinity and organic matter content. Communications in Biometry Crop Science11:98-104. Chen, P. & Tsay, T. (2006) Effect of crop rotation on Meloidogyne spp. and Pratylenchus spp. populations in strawberry fields in Taiwan. Journal of Nematology, 38:339. Chihani-Hammas, N., Hajji-Hedfi, L., Regaieg, H., Larayedh, A., Badiss, A., Qing, Y. & Najet, H.-R. (2018) First report of Pratylenchus vulnus associated with apple in Tunisia. Journal of Nematology, 50(4):579-586. Chitambar, J. & Raski, D. (1985) Life history of Pratylenchus vulnus on carrot discs. Journal of Nematology, 17:235. Choshali, A. H., Seraji, A., Rezaee, S. & Shirinfekr, A. (2013) The relationship between soil pH and population level of Pratylenchus loosi in tea plantations of Iran. Archives of Phytopathology Plant Protection Science, 46:1384-1392. Ciancio, A. (1995) Observations on the nematicidal properties of some mycotoxins. Fundamental Applied Nematology, 18:451-454. Dauphinais, N., Vandal, M., Gagnon, A.-È., Bélair, G., Véronneau, P.-Y. & Mimee, B. (2018) Development and application of a multiplex qPCR method for the simultaneous detection and quantification of Pratylenchus alleni and P. penetrans in Quebec, Canada. Plant Disease, 102:970-976. De Luca, F., Troccoli, A., Duncan, L. W., Subbotin, S. A., Waeyenberge, L., Coyne, D. L., Brentu, F. C. & Inserra, R. N. (2012) Pratylenchus speijeri n. sp.(Nematoda: Pratylenchidae), a new root-lesion nematode pest of plantain in West Africa. Nematology, 14:987-1004. de Man, J. G. (1877) Onderzoekingen over vrij in de aarde levende Nematoden. Derycke, S., Vanaverbeke, J., Rigaux, A., Backeljau, T. & Moens, T. (2010) Exploring the use of cytochrome oxidase c subunit 1 (COI) for DNA barcoding of free-living marine nematodes. PLOS ONE, 5:13716. Donald, C. & Clark, A. (1983) Surface features in the taxonomy of Pratylenchus species. Revue Néinatol, 6:85-98. Doucet, M. & Pinochet, J. (1996) Comparative analysis of morphological and morphometrical characters in six isolates of Pratylenchus vulnus Allen & Jensen, 1951 (Nemata: Tylenchida). Fundamental and Applied Nematology 19:79-84. Doucet, M. E., Lax, P. & Pinochet, J. (1998) Variability of some external characters in Pratylenchus vulnus Allen & Jensen, 1951 (Nernatoda: Tylenchida). Fundamental and Applied Nematology 21:205-206. Eyualem, A. & Blaxter, M. (2003) Comparison of biological, molecular, and morphological methods of species identification in a set of cultured panagrolaimus isolates. Journal of Nematology, 35:119-128. Fang, X. & Barbetti, M. J. (2014) Differential protein accumulations in isolates of the strawberry wilt pathogen Fusarium oxysporum f. sp. fragariae differing in virulence. Journal of Proteomics, 108:223-237. Ferris, J. (1970) Soil temperature effects on onion seedling injury by Pratylenchus penetrans. Journal of Nematology, 2:248. Floyd, R., Abebe, E., Papert, A. & Blaxter, M. (2002) Molecular barcodes for soil nematode identification. Molecular Ecology, 11:839-850. Gebremikael, M. T., Steel, H., Buchan, D., Bert, W. & De Neve, S. (2016) Nematodes enhance plant growth and nutrient uptake under C and N-rich conditions. Scientific Reports, 6:32862. George, C., Kohler, J. & Rillig, M. C. (2016) Biochars reduce infection rates of the root-lesion nematode Pratylenchus penetrans and associated biomass loss in carrot. Soil Biology Biochemistry, 95:11-18. Hafez, S., Al-Rehiayani, S., Thornton, M. & Sundararaj, P. (1999) Investigation-research: Differentiation of two geographically isolated populations of Pratylenchus neglectus based on their parasitism of potato and interaction with Verticillium dahliae. Nematropica, 29:25-36. Handoo, Z. A. & Golden, A. M. (1989) A key and diagnostic compendium to the species of the genus Pratylenchus Filipjev, 1936 (lesion nematodes). Journal of Nematology, 21:202. Hu, C. & Chu, H. (1964) The nematode investigation in sugarcane field of Taiwan (II). Taiwan Sugar Res. Inst, 33:63-82. Huang, Y.-T., Yang, J.-i., Chrobak, M. & Borneman, J. (2014) PRISE2: Software for designing sequence-selective PCR primers and probes. BMC Bioinformatics, 15:317. Hummer, K. E., Nathewet, P. & Yanagi, T. (2009) Decaploidy in Fragaria iturupensis (Rosaceae). American Journal of Botany, 96:713-716. Inomoto, M. M., Goulart, A., Machado, A. C. & Monteiro, A. R. (2001) Effect of population densities of Pratylenchus brachyurus on the growth of cotton plants. Fitopatologia Brasileira, 26:192-196. Inserra, R. & Vovlas, N. (1977) Effects of Pratylenchus vulnus on the growth of sour orange. Journal of Nematology, 9:154. Janssen, T., Karssen, G., Orlando, V., Subbotin, S. A. & Bert, W. (2017) Molecular characterization and species delimiting of plant-parasitic nematodes of the genus Pratylenchus from the penetrans group (Nematoda: Pratylenchidae). Molecular phylogenetics Evolution, 117:30-48. Jeong, M. & Kim, S. (1989) Effects of plant callus, temperature, pH, medium and growth regulating substances on the culture of Pratylenchus penetrans and P. vulnus in vitro. Korean Journal of Plant Pathology, 5:359-363. Johnson, D. A. & Santo, G. S. (2001) Development of wilt in mint in response to infection by two pathotypes of Verticillium dahliae and co-infection by Pratylenchus penetrans. Plant Disease, 85:1189-1192. Jones, J. T., Haegeman, A., Danchin, E. G., Gaur, H. S., Helder, J., Jones, M. G., Kikuchi, T., Manzanilla‐López, R., Palomares‐Rius, J. E. & Wesemael, W. M. (2013) Top 10 plant‐parasitic nematodes in molecular plant pathology. Molecular Plant Pathology, 14:946-961. Jorgenson, E., Hyer, A., Garber, R. & Smith, S. N. (1978) Influence of soil fumigation on the Fusarium-root-knot nematode disease complex of cotton in California. Journal of Nematology, 10:228. Kandel, S. L., Smiley, R. W., Garland-Campbell, K., Elling, A. A., Abatzoglou, J., Huggins, D., Rupp, R. & Paulitz, T. C. (2013) Relationship between climatic factors and distribution of Pratylenchus spp. in the dryland wheat-production areas of eastern Washington. Plant Disease, 97:1448-1456. Kawanobe, M., Miyamaru, N., Yoshida, K., Kawanaka, T. & Toyota, K. (2015) Quantification of lesion nematode (Pratylenchus zeae), stunt nematode (Tylenchorhynchus leviterminalis), spiral nematode (Helicotylenchus dihystera), and lance nematode (Hoplolaimus columbus), parasites of sugarcane in Kitadaito, Okinawa, Japan, using real-time PCR. Nematological Research (Japanese Journal of Nematology), 45:35-44. Kazuya, H. T., KAWAMURA, (1972) Incidence of complex disease caused by Pratylenchus penetrans or P. coffeae and Fusarium wilt fungus in tomato seedlings The Technical bulletin of Faculty of Horticulture, Chiba University., 20. Koike, S., Kirkpatrick, S. & Gordon, T. (2009) Fusarium wilt of strawberry caused by Fusarium oxysporum in California. Plant Disease, 93:1077-1077. Kuniyasu, K. & Seinhorst, J. (1971) Interaction of Pratylenchus Penetrans and Fusarium oxysporum forma pisi race 2 and of rotylenchus uniformis and F. Oxysporum f. pisi race 1 on peas. Nematologica, 17:444-452. Lamberti, F., D'Addabbo, T., Sasanelli, N. & Carella, A. (2001) Control of Pratylenchus vulnus in stone fruit nurseries. Mededelingen, 66:629-632. LaMondia, J. (1999) Effects of Pratylenchus penetrans and Rhizoctonia fragariae on vigor and yield of strawberry. Journal of Nematology, 31:418. Lax, P., Di Rienzo, J., Baujard, P., Doucet, M. & Pinochet, J. (2004) Inter-population variability in Pratylenchus vulnus Allen & Jensen, 1951 (Nematoda: Tylenchida). Nematology, 6:257-260. Liu, X., Wang, H., Lin, B., Tao, Y., Zhuo, K. & Liao, J. (2017) Loop-mediated isothermal amplification based on the mitochondrial COI region to detect Pratylenchus zeae. European Journal of Plant Pathology, 148:435-446. Lownsbery, B., Huang, C. & Johnson, R. N. (1967) Tissue culture and maintenance of the root-lesion nematode, Pratylenchus vulnus. Nematologica, 13:390-394. Machado, A., Ferraz, L. & de Oliveira, C. (2007) Development of a species-specific reverse primer for the molecular diagnostic of Pratylenchus brachyurus. Nematropica, 37:249-257. Madan, L. & Jauhari, R. (2007) Effect of soil pH on the population of migratory nematode Pratylenchus penetrans (Cobb, 1917)(Nematoda: Hoplolamidae) on tea plantations in Doon Valley. Journal of Experimental Zoology, India, 10:469-471. Mai, W. (1988) Plant-parasitic nematodes: a pictorial key to genera, Cornell University Press. Mai, W. F. & Lyon, H. H. (1962) Pictorial key to genera of plant parasitic nematodes. Pictorial key to genera of plant parasitic nematodes. Mauza, B. & Webster, J. (1982) Suppression of alfalfa growth by concommitant populations of Pratylenchus penetrans and two Fusarium species. Journal of Nematology, 14:364. Mauza, B. E. (1976) The nematode-fungal interaction of Pratlyenchus penetrans and Fusarium species on alfalfa. PhD Thesis. Simon Fraser University. Theses (Dept. of Biological Sciences. May, D. B., Johnson, W. A., Zuck, P. C., Chen, C. C. & Dyer, A. T. (2016) Assessment and management of root lesion nematodes in Montana wheat production. Plant Disease, 100:2069-2079. Mckinley, R. T. & Talboys, P. W. (1979) Effects of Pratylenchus penetrans on development of strawberry wilt caused by Verticillium dahliae. 92:347-357. Michell, R. E. P., W. M. (1971) Influence of Pratylenchus brachyurus on the Incidence of Fusarium Wilt in Cotton. Phytopathology, 62. Mirocha, C. J., Abbas, H., Kommedahl, T. & Jarvis, B. (1989) Mycotoxin production by Fusarium oxysporum and Fusarium sporotrichioides isolated from Baccharis spp. from Brazil. Appl. Environ. Microbiol., 55:254-255. Mitsui, Y., Yokozawa, R. & Ichinohe, M. (1975) Effect of temperature and pH on the propagation of Pratylenchus culturing with alfalfa callus, tissues. Japanese Journal of Nematology, 5:48-55. Mizukubo, T. & Adachi, H. (1997) Effect of temperature on Pratylenchus penetrans development. Journal of Nematology, 29:306. Mizukubo, T. & Sano, Z. i. (1997) Pratylenchus coffeae virulent races on sweetpotato. Sweetpotato Research Front, 4. MIzukubo, T. (1990) Pictogram analysis of spear length, lip region diameter and tail morphology in cohabiting Pratylenchus penetrans and P. vulnus (Tylenchida: Pratylenchidae). Japanese Journal of Nematology, 20:51-55. Mokrini, F., Waeyenberge, L., Viaene, N., Andaloussi, F. A. & Moens, M. (2013) Quantitative detection of the root-lesion nematode, Pratylenchus penetrans, using qPCR. European Journal of Plant Pathology, 137:403-413. Nash, S. M. & Snyder, W. C. (1962) Quantitative estimations by plate counts of propagules of the bean root rot Fusarium in field soils. Phytopathology, 52. Norton, D. C. (1989) Abiotic soil factors and plant-parasitic nematode communities. Journal of Nematology, 21:299. Norton, D. C. (1979) Relationship of physical and chemical factors to populations of plant-parasitic nematodes. Annual Review of Phytopathology 17.1: 279-299. Nunn, G. B. ( 1992) Nematode molecular evolution: an investigation of evolutionary patterns among nematodes based upon DNA sequences. PhD Thesis. University of Nottingham. Okamoto, H., Fujii, S., Kato, K., and Yoshioka, A (1970) A new strawberry disease ‘Fusarium wilt’. Plant Protection Science, 24:231-235. Oliveira, C. M., Blok, V., Neilson, R., Mróz, T. & Roberts, D. (2017) Hydrolysis probe-based PCR for detection of Pratylenchus crenatus, P. neglectus and P. penetrans. Nematology, 19:81-91. Pereira, T. J. & Baldwin, J. G. (2016) Contrasting evolutionary patterns of 28S and ITS rRNA genes reveal high intragenomic variation in Cephalenchus (Nematoda): Implications for species delimitation. Molecular Phylogenetics Evolution, 98:244-260. Pinochet, P., Cenis, J., Fernandez, C., Doucet, M. & Maruli (1994) Reproductive fitness and random amplified polymorphic DNA variation among isolates of Pratylenchus vulnus. Journal of Nematology, 26:271. Roman, J. & Hirschmann, H. (1969) Morphology and morphometrics of six species of Pratylenchus. Journal of Nematology, 1:363. Saeed, I., MacGuidwin, A. & Rouse, D. (1998) Effect of initial nematode population density on the interaction of Pratylenchus penetrans and Verticillium dahliae on'Russet burbank'potato. Journal of Nematology, 30:100. Schmitt, D. P. & Barker, K. R. (1981) Damage and reproductive potentials of Pratylenchus brachyurus and P. penetrans on soybean. Journal of Nematology, 13:327-332. Schmitt, D. P. & Sipes, B. S. (1998) Plant-parasitic nematodes and their management. Cooperative Extension Service, College of Tropical Agriculture & Human Resources, University of Hawaii at Manoa. Schneider, C. A., Rasband, W. S. & Eliceiri, K. W. (2012) NIH Image to ImageJ: 25 years of image analysis', Nature methods 9(7): 671-675, PMID 22930834. Schulz, A. E., Eisenhauer, N. & Cesarz, S. (2018) Testing soil nematode extraction efficiency using different variations of the Baermann funnel method. BioRxiv:318691. Shah, M. M. & Mahamood, M. Introductory Chapter: Nematodes-A Lesser Known Group of Organisms. In, Nematology-Concepts, Diagnosis and Control. IntechOpen, 2017. Subbotin, S. A., Vierstraete, A., De Ley, P., Rowe, J., Waeyenberge, L., Moens, M. & Vanfleteren, J. R. (2001) Phylogenetic relationships within the cyst-forming nematodes (Nematoda, Heteroderidae) based on analysis of sequences from the ITS regions of ribosomal DNA. Molecular Phylogenetics and Evolution, 21:1-16. Suga, H., Hirayama, Y., Morishima, M., Suzuki, T., Kageyama, K. & Hyakumachi, M. (2013) Development of PCR Primers to Identify Fusarium oxysporum f. sp. fragariae. Plant Disease, 97:619-625. Talwana, H., Butseya, M. & Tusime, G. (2008) Occurence of plant parasitic nematodes and factors that enhance population build-up in cereal-based cropping systems in Uganda. African Crop Science Journal, 16. Toung, M. C. (1963) Some parasitic nematodes associated with citrus in Taiwan. Plant Prot. Bull. 5:17- 22. (in Chinese with English abstract). Townshend, J. & Marks, C. (1977) Temperature and the expression of damage caused by Pratylenchus penetrans in flue-cured tobacco. Nematologica, 23:29-32. Townshend, J. & Wolynetz, M. (1991) Penetration of celery and alfalfa roots by Pratylenchus penetrans as affected by temperature. Journal of Nematology, 23:194. Townshend, J. L. (1984) Anhydrobiosis in Pratylenchus penetrans. Journal of Nematology, 16:282-289. Towson, A. & Lear, B. (1982) Effect of temperature on reproduction and motility of Pratylenchus vulnus. Journal of Nematology, 14:602. Tsang, W. Y. & Lemire, B. D. (2002) Mitochondrial genome content is regulated during nematode development. Biochemical and Biophysical Research Communications, 291:8-16. Upadhaya, A., Yan, G., Pasche, J. & Kalil, A. (2018) Occurrence and distribution of vermiform plant-parasitic nematodes and the relationship with soil factors in field pea (Pisum sativum) in North Dakota, USA. Nematology, 1:1-13. Van den Bergh, I., Tuyet, N. T., Nguyet, D. T. M., De Waele, D. & Nhi, H. H. (2006) Influence of Pratylenchus coffeae and Meloidogyne spp. on plant growth and yield of banana (Musa spp.) in Vietnam. Nematology, 8:265-271. Vaz, C. M., Galbieri, R., Crestana, S., Silva, J. F., Resende, J. M. & Andrade, A. L. (2016) Soil physical attributes affecting cotton productivity and nematode population in mato grosso state,Brazil. Willis, C. (1972) Effects of soil pH on reproduction of Pratylenchus penetrans and forage yield of alfalfa. Journal of Nematology, 4:291. Winks, B. L. & Williams, Y. N. (1965) A wilt of strawberry caused by a new form of Fusarium oxysporum. Queensland J Agric Anim Sci, 22:475-479. Yan, G., Smiley, R. W. & Okubara, P. A. (2012) Detection and quantification of Pratylenchus thornei in DNA extracted from soil using real-time PCR. Phytopathology, 102:14-22. Yavuzaslanoglu, E., Elekcioglu, H. I., Nicol, J. M., Yorgancilar, O., Hodson, D., Yildirim, A. F., Yorgancilar, A. & Bolat, N. (2012) Distribution, frequency and occurrence of cereal nematodes on the Central Anatolian Plateau in Turkey and their relationship with soil physicochemical properties. Nematology, 14:839-854. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72710 | - |
dc.description.abstract | 根腐線蟲 (Pratylenchus spp.) 寄生超過300種植物,在全球重要作物造成嚴重經濟損失。本實驗室2017年於苗栗大湖地區之草莓園GS及KDL發現大量根腐線蟲,對其進行型態量測及分子鑑定,確認物種為 P. vulnus (草莓根腐線蟲),為台灣新紀錄種。本研究以接種試驗確認其病原性後,為提供快速準確之鑑定及監測方法,開發專一性的分子即時定量檢測技術。此外,對此種根腐線蟲於草莓園中的族群消長進行超過一年的監測,透過分析生物性與非生物性因子,探討與田間病原性真菌之交互作用可能性,並建立適用田間之族群預測模型。本研究中發現,大湖草莓園所分離得之族群型態量測平均值符合該物種最早被報導的California族群數據範圍,但與最新被報導的Lorca Chiha族群,則有雄成蟲數據差異。四個分子標記區域進行基因親緣性分析的結果,均確認物種分類地位與型態測量結果,且暗示本族群已特化獨立於其他地區族群。接種試驗結果顯示P. vulnus確實能在草莓根部繁殖並造成明顯之壞疽病徵,但對植株的生長勢並無影響。運用粒線體COI基因作為為標的所開發的專一性PCR引子對PvC2978F / PvC2978R,經測試及優化後,無論以土壤或根部組織的全核酸樣本進行試驗,均能成功進行目標偵測。以其作為基礎,後搭配專一性探針PvC-pr的使用,亦開發即時定量的PCR偵測技術。此偵測技術的準確性,經130個樣本的檢測定量分析與改良式柏門氏漏斗分離法相比,顯示兩方法所得的數量雖有正相關性,但本方法偵測所得結果最多可多15540倍,凸顯本qPCR技術之準確性。在田間族群動態分析方面,非生物性因子中僅有每月土壤平均溫度與每月根腐線蟲密度有顯著負相關性。族群密度會隨著草莓植株種植天數 (Day after planting, DAP) 增加而明顯上升,且DAP與每月土壤平均溫度有負相關性,並呈現二項式分佈。以DAP:112作為分組切線,成功建立兩組在不同DAP情況下,能以每月土壤平均溫度預測P. vulnus田間族群密度之模型。此外,以本研究另外開發之專一性qPCR技術,對田區樣本中草莓尖鐮孢菌(Fusarium oxysporum f. sp. fragariae)進行定量,發現該真菌病原與之P. vulnus族群密度有正相關性,暗示交互作用的存在。然而,雙重病原的接種交互作用的溫室接種試驗中,於草莓生長勢的分析,數據結果僅顯示草莓尖鐮孢菌為主要效應。 | zh_TW |
dc.description.abstract | Pratylenchus spp. parasitize more than 300 plant species globally and cause damages and enormous economic loss on important crops. In 2017, large amount of Pratylenchus spp. nematodes were discovered in two strawberry fields in the Dahu area of Miaoli county. Both morphological measurement and molecular identification results indicated the nematode species as Pratylenchus vulnus; a new record species in Taiwan. Inoculation of the nematode on strawberry had confirmed its pathogenicity. To establish an accurate and quick method for diagnosis and monitoring, a specific quantitative real-time PCR method was developed. Next, the population dynamic of P. vulnus in the strawberry field was monitored for over a year and the correlations between biotic and abiotic factors and the nematode density was studied. Finally, a population density prediction model was built and the interaction between the nematode and pathogenic fungi was analyzed.This study found that the body measurement means of P. vulnus Taiwan isolate fall in the same range of the California isolate. On the other hand, the data from male body measurements are somehow different to the Lorca Chiha isolate. The California isolate and the Lorca Chiha isolate are the earliest and the latest P. vulnus group being reported. sequence analysis of four molecular markers not only confirmed the morphological identification, but also further implied the possibility of an isolated evolution of P. vulnus Taiwan isolate. The inoculation of P. vulnus on strawberry confirmed its capability of causing significant lesion symptoms on the root but does not influence the plant growth. For the detection assay development, a specific PCR primer set PvC2978F / PvC2978R was designed to target the COI gene region of mtDNA. Optimization results showed the successful detection of the target nematode in total DNA of root tissue and soil. With an addition of the specific PvC-pr probe, the quantitative real-time PCR assay was established. The qPCR quantification results of 130 soil samples indicated the positive correlation to the results obtained by Baermann funnel method. However, the data gap is up to 15540 fold-time. From the population dynamic analysis, only the average monthly soil temperature was significantly negatively correlated to the monthly nematode density. The population density significantly was found to increase along with DAP (day after planting). The DAP is negatively correlated to the average monthly soil temperature in binomial distribution. Therefore, when separate the data using DAP:112, two population density prediction models were established; Both models use the monthly soil temperature for P. vulnus density prediction, but are for applications in difference DAP ranges. In addition, with the specific qPCR assay developed in this study, the amount of the strawberry pathogenetic fungus Fusarium oxysporum f. sp. fragariae was quantified in the soil samples. The result showed a positive correlation of the fungus and the population density of P. vulnus. However, the result of interaction experiment only revealed the impact of the fungus on the strawberry growth. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T07:04:11Z (GMT). No. of bitstreams: 1 ntu-108-R05645007-1.pdf: 18025776 bytes, checksum: d580217f32a3be243891c2564eefe54e (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員會審定書
............................................................................................................................i 中文摘要 ............................................................................................................................ii 英文摘要 ............................................................................................................................iv 目錄 ............................................................................................................................vii 表次索引 ............................................................................................................................x 圖次索引 ............................................................................................................................xi 壹、引言 ............................................................................................................................1 ㄧ、根腐線蟲 Pratylenchus sp. ............................................................................................................................1 二、根腐線蟲鑑定 ............................................................................................................................2 三、根腐線蟲分子檢測技術 ............................................................................................................................3 四、臺灣之根腐線蟲 ............................................................................................................................3 五、根腐線蟲與田間非生物性因子 ............................................................................................................................3 六、根腐線蟲田間族群密度預測模型 ............................................................................................................................4 七、田間生物性因子對根腐線蟲族群動態的影響 ............................................................................................................................5 八、草莓萎凋病 ............................................................................................................................6 九、台灣草莓種植現況 ............................................................................................................................7 貳、材料與方法 ............................................................................................................................9 一、調查地區與採集方法 ............................................................................................................................9 二、線蟲田間族群分析 ............................................................................................................................9 A. 根部組織染色 ............................................................................................................................9 B. 線蟲分離 ............................................................................................................................10 三、田間非生物性環境因子資料收集 ............................................................................................................................10 四、根腐線蟲鑑定與培養 ............................................................................................................................12 A. 型態鑑定 ............................................................................................................................12 B. 單隻線蟲總體核酸萃取 ............................................................................................................................13 C. 分子鑑定 ............................................................................................................................14 i. PCR 反應條件與膠體電泳 ............................................................................................................................14 ii. 目標PCR 產物純化 ............................................................................................................................15 iii. 目標片段質體構築 ............................................................................................................................16 iv. 質體純化 ............................................................................................................................16 v. 序列及親緣樹分析 ............................................................................................................................17 D. 線蟲純培養 ............................................................................................................................18 五、草莓根腐線蟲分子偵測技術開發 ............................................................................................................................18 A.專一性PCR引子對及qPCR 探針之設計 ............................................................................................................................18 B. PCR 及qPCR 之反應條件、試劑及體積 ............................................................................................................................19 C.專一性測試 ............................................................................................................................20 D.敏感性、應用性及qPCR 迴歸曲線建立 ............................................................................................................................20 E.草莓園田間土壤草莓根腐線蟲族群密度偵測 ............................................................................................................................21 六、草莓鐮孢菌分子偵測技術開發 ............................................................................................................................21 A.真菌來源及培養 ............................................................................................................................21 B.田間樣本檢測 ............................................................................................................................22 C.專一性引子對及probe 設計 ............................................................................................................................22 D.qPCR 反應技術之優化 ............................................................................................................................23 E.草莓園田間土壤鐮孢菌族群密度偵測 ............................................................................................................................24 七、接種試驗及試驗數據收集之方法 ............................................................................................................................24 A.接種病原及材料 ............................................................................................................................24 B.接種試驗 ............................................................................................................................24 C.試驗數據收集 ............................................................................................................................25 八、統計分析 ............................................................................................................................26 A.田間因子相關性分析 ............................................................................................................................26 B.田間族群密度預測迴歸曲線建立 ............................................................................................................................26 C.草莓根腐線蟲與鐮孢菌之交互作用試驗分析 ............................................................................................................................27 參、結果 ............................................................................................................................28 一、樣本收集與病徵判斷 ............................................................................................................................28 二、根腐線蟲鑑定 ............................................................................................................................28 三、草莓根腐線蟲接種試驗 ............................................................................................................................30 四、草莓根腐線蟲之檢測技術優化及測試 ............................................................................................................................31 A.一般性PCR 優化及測試 ............................................................................................................................31 B. qPCR (quantitative PCR)優化及測試 ............................................................................................................................32 五、草莓根腐線蟲與非生物性因子相關性 ............................................................................................................................33 六、草莓根腐線蟲族群密度預測模型 ............................................................................................................................34 七、草莓根腐線蟲與生物性因子相關性 ............................................................................................................................35 八、草莓根腐線蟲與草莓鐮孢菌交互作用接種試驗 ............................................................................................................................36 肆、討論 ............................................................................................................................38 伍、參考文獻 ............................................................................................................................46 陸、圖表 ............................................................................................................................56 | |
dc.language.iso | zh-TW | |
dc.title | 草莓根腐線蟲之鑑定與偵測技術開發及其田間族群動態之研究 | zh_TW |
dc.title | The identification, detection assay development and population dynamic study of Pratylenchus vulnus | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鍾嘉綾(Chia-Lin Chung),林乃君(Nai-Chun Lin),劉力瑜,陳殿義 | |
dc.subject.keyword | 草莓根腐線蟲,分子檢測技術,族群動態,預測模型,草莓尖鐮孢菌, | zh_TW |
dc.subject.keyword | Pratylenchus vulnus,molecular diagnostic method,population dynamics,prediction model,Fusarium oxysporum f. sp. fragariae, | en |
dc.relation.page | 96 | |
dc.identifier.doi | 10.6342/NTU201901883 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-07-29 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物醫學碩士學位學程 | zh_TW |
Appears in Collections: | 植物醫學碩士學位學程 |
Files in This Item:
File | Size | Format | |
---|---|---|---|
ntu-108-1.pdf Restricted Access | 17.6 MB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.