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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 詹長權(Chang-Chuan Chan) | |
dc.contributor.author | Yi-Ting Hsieh | en |
dc.contributor.author | 謝億廷 | zh_TW |
dc.date.accessioned | 2021-06-17T07:02:38Z | - |
dc.date.available | 2024-08-26 | |
dc.date.copyright | 2019-08-26 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-07-31 | |
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Vineis P, van Veldhoven K, Chadeau-Hyam M, Athersuch TJ. Advancing the application of omics-based biomarkers in environmental epidemiology. Environ Mol Mutagen 2013; 54: 461-7. Wang CW, Liao KW, Chan CC, Yu ML, Chuang HY, Chiang HC, et al. Association between urinary thiodiglycolic acid level and hepatic function or fibrosis index in school-aged children living near a petrochemical complex. Environ Pollut 2019; 244: 648-656. Wei B, Yu J, Kong C, Li H, Yang L, Guo Z, et al. An investigation of the health effects caused by exposure to arsenic from drinking water and coal combustion: arsenic exposure and metabolism. Environmental Science and Pollution Research 2017; 24: 25947-25954. Weng HH, Tsai SS, Chiu HF, Wu TN, Yang CY. Association of childhood leukemia with residential exposure to petrochemical air pollution in taiwan. Inhal Toxicol 2008; 20: 31-6. Wilhelm M, Pesch B, Wittsiepe J, Jakubis P, Miskovic P, Keegan T, et al. 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Int J Environ Res Public Health 2014b; 11: 7319-32. 邊緯緒, 詹長權: 六輕離島工業區周界之懸浮微粒及附近居民尿中As、Hg濃度之評估研究. 國立台灣大學職業醫學與工業衛生研究所碩士論文 2011. 林右翊, 詹長權: 火力發電廠周界空氣微粒與兒童尿中As、Hg濃度之評估研究. 國立台灣大學職業醫學與工業衛生研究所碩士論文 2010. 沈育正, 詹長權: 六輕石化工業區附近成人癌症發生之探討. 國立台灣大學職業醫學與工業衛生研究所碩士論文 2014. 莊明潔, 詹長權: 彰化縣大城鄉居民慢性腎臟病與六輕工業區聚力相關性之研究. 國立台灣大學職業醫學與工業衛生研究所碩士論文 2018. 詹長權、李永凌、洪壽宏 (民101) 100年度沿海地區空氣汙染物及環境健康世代研究計畫。雲林縣環保局委託之計畫 (編號:YLEPB-100-029) 詹長權、王崇禮、陳永銘、陳崇裕、蔡政憲 (民103) 102年度沿海地區環境流行病學計畫。雲林縣環保局委託之計畫 (編號: 10216) | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72644 | - |
dc.description.abstract | 台灣中部雲林地區之六輕石化工業區為台灣最大的工業區,該工業區被認為是台灣中部空氣污染物的主要排放源,其內部包含石油裂解廠、燃煤發電廠等,然而在過去研究中證實,石化工業與燃煤發電廠皆是重要的砷暴露來源,亦證實居住在六輕石化工業區周圍之居民尿中總砷含量較遠離工業區的居民高,且更在相關的流行病學研究報告指出該地區兒童哮喘與成人癌症等不良健康效應,但這些研究並沒有討論居民尿液中無機砷的暴露、代謝產物以及其可能導致的相關早期不良健康影響。因此,我們的研究目的是透過分析居民尿液中各砷物種之含量和代謝體學,來了解居住在六輕石化工業區周圍之老人與小孩的無機砷暴露、代謝情況以及早期不良健康指標。
我們的研究個案為2009 -2012年居住鄰近六輕石化工業區的 79 名老人 (>55歲) 與 81 名小孩 (9-15歲),收案內容包含尿液及生活習慣問卷調查,並限制至少居住在當地五年以上者,共 160 位,使用變異數分析以及 Chi-square test 進行研究目標之人口學統計分析。尿液樣本中砷物種分析使用 HPLC (High performance liquid chromatography, HPLC) 串聯 ICP-MS (Inductively coupled plasma mass spectrometry, ICPMS),共分析四種砷物種,分別為無機砷的As3+、As5+ 和有機砷的DMA (Dimethylarsinic acid, DMA)、MMA (Monomethylarsonic acid, MMA),並再利用肌酐酸(creatinine) 校正尿液濃度。我們使用三種砷物種之甲基化代謝指數,分別為主要甲基化指數 (Primary methylation index, PMI)、次要甲基化指數 (Secondary methylation index, SMI) 以及 (DMA+MMA)/(As3++As5+),其中 PMI 為 MMA 與 (As3++As5+) 之比值,而 SMI 為 DMA與 MMA 之比值,利用此三個指數來估算砷物種甲基化的程度。代謝體學資料運用線上統計軟體 MetaboAnalyst 4.0,來研究尿中砷物種暴露與各砷物種激化相關的特定代謝物之間的關聯。 我們的研究發現,在小孩組別當中,高暴露區的居民尿中無機砷 (As3++As5+ 總和) 濃度顯著高於低暴露區。在無機砷的百分比上,高暴露區也顯著高於低暴露區,而在老人組別當中,高暴露區居民尿中 As5+ 顯著高於低暴露區。砷物種甲基化指標顯示,小孩高暴露區相較低暴露組之 PMI 、有機砷與無機砷之比值顯著較低。而在老人組別當中,我們僅發現高暴露組之 PMI 顯著低於低暴露組。我們的研究也發現兒童高暴露組中,數個與無機砷相關甲基化途徑代謝物的下調,包括Glyceraldehyde-3-phosphate, Glutamic acid, Methionine, Glutaric acid, Fumaric acid, Glycine,我們亦發現與無機砷所造成體內氧化壓力上升之相關代謝體的上調,包括 2-keytrobutyric acid, L-Valine, Succinic acid, Hippuric acid, Serine。 本研究之結論為居住在六輕石化工業區附近的居民,其有較高的無機砷暴露,且相較於居住遠離六輕的居民,其無機砷的甲基化代謝效率較差,更在代謝體學資料中發現,與砷物種甲基化能力相關之代謝體下調以及與無機砷所造成體內氧化壓力相關之代謝體上調的情況。因此,統整以上,我們的無機砷暴露評估研究,提供了過去許多砷暴露之不良健康影響相關流行病學研究之重要證據。 | zh_TW |
dc.description.abstract | The No. 6 Naphtha Cracker Complex in the Yunlin of central Taiwan is the largest industrial zone in Taiwan, which includes petrochemical complex and coal-fired power plants. Previous studies have confirmed that this industrial zone is the main arsenic exposure source in central Taiwan. Studies also found total urinary arsenic concentrations among residents living nears this industrial complex were comparatively higher that those living farther away from the complex. Adverse health effects reported in previous epidemiological studies in this area include children’s asthma and adult’s cancers. These studies, however, did not report exposures of inorganic arsenic, which is more toxic than organic arsenic, and metabolites of inorganic arsenic as well as early health effects. Our study objective is to know whether inorganic arsenics exposures can help explain adverse health effects among elderly and children near No. 6 Naphtha Cracker Complex by analyzing study subjects’ urinary arsenic species and metabolomics.
Our study includes 160 subjects of 79 elderly (>55 years old) and 81 children (9-15 years old) residents living near No. 6 Naphtha Cracker Complex from 2009 to 2012. All of them have completed questionnaire surveys about living habits and factors related to exposure, and both of them lived in the local area at least five years. We used the variance analysis and Chi-square test to conduct demographic statistical analysis. Arsenic species in urine were analyzed by HPLC-ICPMS (High performance liquid chromatography-inductively coupled plasma mass spectrometry, HPLC-ICPMS) to differentiate four arsenic species, As3+, As5+, DMA (Dimethylarsinic acid, DMA), MMA (Monomethylarsonic acid, MMA), and reported as creatinine-adjust urine concentrations. We used three index, namely primary methylation index (PMI), secondary methylation index (SMI), and ratio of (DMA+MMA) /(As3++As5+), where PMI is the ratio of (DMA+MMA) to (As3++As5+ +DMA+MMA), and SMI is the ratio of DMA to (DMA+MMA) to estimate methylation metabolism of arsenic species. MetaboAnalyst 4.0 was used to investigate the association between urinary arsenic species exposure and specific metabolites associated with methylation of various arsenic species. Our study found that children’s inorganic arsenic concentrations, sum of As3+ and As5+, in the high exposure group were significantly higher than the low exposure group. The percentage of inorganic arsenic in total arsenics in the high exposure group was also significantly higher than low exposure group. By contrast, among the elderly, only As5+ concentrations were significantly higher for the high exposure group compared to the low exposure group. Arsenic methylation index showed that the children in high exposure group had lower PMI and the ratio of organic arsenic to inorganic arsenic than those in the low exposure group. As for the elderly, we only found lower PMI in the high exposure group compared to the low exposure group. We identified down-regulation of several inorganic arsenic-related methylation pathway metabolites, including Glyceraldehyde-3-phosphate, Glutamic acid, Methionine, Glutaric acid, Fumaric acid, Glycine showed among children in the high exposure group. We also found up-regulation of some metabolites of oxidative stress caused related to inorganic arsenic, including 2-keytrobutyric acid, L-Valine, Succinic acid, Hippuric acid, Serine. We conclude that residents living near the petrochemical complex have higher inorganic arsenic exposure and poorer methylation metabolism of inorganic arsenics than those living farther away. According to the study, the high exposure group had higher inorganic arsenic exposure than the low exposure group. Metabolites of methylation were downregulated while those of oxidative stress were upregulated among children with high inorganic arsenic exposures. Our refined exposure assessment provided evidence to support that inorganic arsenics played an important role of causing adverse health effects reported in previous epidemiological studies. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T07:02:38Z (GMT). No. of bitstreams: 1 ntu-108-R06841009-1.pdf: 2298617 bytes, checksum: ec965f6103fb7139a7503415d8a71772 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 目錄
中文摘要 I 英文摘要 III 目錄 V 圖目錄 VII 表目錄 VIII 第一章 文獻探討 1 1.1 砷 (Arsenic, As) 1 1.2 砷物種之暴露 2 1.3 砷物種代謝途徑 3 1.4 砷物種代謝相關之代謝體學 6 1.5 砷物種之毒性 7 1.6 石化工業區與燃煤發電廠之無機砷汙染 8 1.7 研究目的 10 第二章 材料與方法 11 2.1 研究架構 11 2.2 研究地區和對象 12 2.3 研究問卷與生物檢體蒐集 14 2.4 溶劑與藥品配置 15 2.5 樣本配置 16 2.6 砷物種分析 17 2.7 高效能液相層析儀條件設定 18 2.8 感應耦合電漿質譜儀條件設定 18 2.9 分析品質保證與品質管制 20 2.10 代謝體資料庫 21 2.11 統計分析 22 第三章 結果 23 3.1研究對象之基本統計資料 23 3.2 研究對象尿中砷物種濃度 25 3.3 砷物種相關之代謝體 29 第四章 討論 32 4.1石化工業區砷物種之暴露 32 4.2 居民體內之砷物種分布 36 4.3 砷物種甲基化代謝途徑與氧化壓力相關之代謝體學 38 第五章 結論與建議 41 第六章 參考文獻 42 第七章 附錄 46 附錄一 As檢量線配置(As5+、As3+、DMA、MMA) 46 附錄二 尿液中重金屬分析之ICP-MS上機標準作業程序 48 附錄三 Agilent HPLC-ICPMS 簡易操作手冊 53 | |
dc.language.iso | zh-TW | |
dc.title | 六輕工業區周圍居民無機砷暴露之研究 | zh_TW |
dc.title | Inorganic arsenic exposure of residents living near the
No. 6 Naphtha Cracking Complex. | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃耀輝(Yaw-Huei Hwang),王淑麗(Shu-Li Wang) | |
dc.subject.keyword | 無機砷,砷甲基化,代謝體學,暴露評估, | zh_TW |
dc.subject.keyword | Inorganic arsenic,Arsenic methylation,Metabolomics,Petrochemical,Exposure assessment, | en |
dc.relation.page | 58 | |
dc.identifier.doi | 10.6342/NTU201902217 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-07-31 | |
dc.contributor.author-college | 公共衛生學院 | zh_TW |
dc.contributor.author-dept | 職業醫學與工業衛生研究所 | zh_TW |
顯示於系所單位: | 職業醫學與工業衛生研究所 |
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