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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 洪傳揚 | |
| dc.contributor.author | Ying-Hua Wu | en |
| dc.contributor.author | 吳映樺 | zh_TW |
| dc.date.accessioned | 2021-06-17T07:02:20Z | - |
| dc.date.available | 2019-08-06 | |
| dc.date.copyright | 2019-08-06 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019-07-30 | |
| dc.identifier.citation | 戶刈義次 (1963) 作物學試驗法 東京農業技術學會印行 第159-176頁
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72632 | - |
| dc.description.abstract | 植物體內的穀胱甘肽 (GSH) 在平衡氧化還原狀態、抵抗非生物逆境及傳遞分子訊息等方面皆扮演關鍵的角色。實驗室過去的研究發現弱化GR1 表達會抑制水稻側根生成及伸長，本研究進一步釐清 GSH 含量以及氧化還原狀態對水稻幼苗根系發育與 Auxin 傳輸之影響。試驗中對水稻幼苗外加 GSH 或 GSSG 處理都會顯著縮短水稻種子根主根長度，並同時減少側根數量和長度，分析 OsGR1弱化表現與大量表現的水稻植株根部，也發現兩轉殖株的主根均較野生型短，其中大量表現型主根最短且側根數量和密度也最低，這些結果顯示GSH / GSSG 比例的改變，會抑制種子根主根和側根生長；以受 Auxin 誘導之 DR5::GUS 轉殖水稻進行組織化學染色分析，結果顯示對照組的 GUS 主要分佈在靠近主根和側根根冠、根尖及延長部，而無論外加 GSH 或 GSSG 都會擴大 GUS 在種子根內之分佈位置；以 GSH 處理 GR1-Ri x DR5::GUS 雜交水稻，能增加主根和側根的 Auxin 分佈且原來生長受抑制的側根能恢復至野生型的水準，顯示 GSH 氧化還原狀態在根部內生長素的傳輸及分佈中扮演關鍵的角色。另一方面，外加 GSH 生合成抑制劑 BSO 會抑制內生 GSH 總生成量及側根生長，而不會影響主根伸長。但隨著 BSO濃度增加，GUS 分佈位置逐漸縮小，最後侷限於近延長部與其附近側根中。同時 GUS 的活性也隨著 BSO 處理濃度增加而逐漸降低，中柱兩側的向基運輸 (Basipatel transport) 明顯減弱並造成主根蜷曲、側根發育嚴重缺陷，顯示 GSH 生合成總量對根部內生長素的傳輸及分佈佔有相當重要的地位，而大量表現GR1 (GR1-OE x DR5::GUS) 能減緩 BSO 對 GUS 分佈及側根長度的抑制。藉由RT-PCR分析 Auxin 相關基因的表現，結果發現水稻內不同 GSH 總量或 GSH / GSSG 比例對運輸載體基因 (OsAUX1 和 OsPINs) 和生合成酵素基因 (OsYUCCA5) 都有迥異的表現差異。以上結果說明水稻內 GSH 含量多寡與氧化還原比例都會影響根系內 Auxin 的生合成、運輸與分佈，進而調控主根和側根的生長與發育。 | zh_TW |
| dc.description.abstract | Glutathione plays a critical role in plant, for example, balancing redox status, resisting abiotic stress, and signaling molecular message. Based on our past research, we found that knockdown of OsGR1 inhibited lateral root formation and elongation. This study clarified the effect of GSH level and redox ratio on root development and auxin transport in rice seedling. In seminal root, the primary root length shortened, lateral root number and length decreased significantly after treating with GSH and GSSG. Phenotyping analysis showed that OsGR1 transformation lines [GR1-Ri (RNA interference) and GR1-OE (Overexpression)] had poorer root development than wild type, especially in overexpression line. These outcomes implied that the changes of GSH / GSSG ratio inhibited primary and lateral root growth. The auxin-inducible promoter DR5 was applied to trace the presence of auxin. In transgenic rice line (DR5::GUS), the distribution of GUS was just located near root caps and root tips of primary and lateral roots. However, regardless of treating with GSH or GSSG, the GUS distributed in whole seminal root evenly. The poor auxin distribution and lateral root length of GR1-knockdown line (GR1-Ri x DR5::GUS) could be recovered by treating with 2 mM GSH, which means that GSH redox status plays an important role in transportation and distribution of auxin. On the other hand, the primary root length wasn’t affected by buthionine sulfoximine (BSO), a GSH biosynthesis inhibitor, but the GSH-lacking event caused a severe inhibition on lateral root number and length. The GUS distribution was confined near root tips and GUS activity was decreased as BSO concentration increased. Meanwhile, the basipetal transport around the stele in root weakened significantly, and caused curly primary root and defective development of lateral root. It showed that GSH content is essential to auxin transport and distribution as well. Overexpression of GR1 alleviated the inhibition on auxin distribution and lateral root growth caused by BSO. The expression of auxin-related genes under different GSH contents and GR expression levels was analyzed through RT-PCR, the results showed that auxin influx and efflux transport carrier genes, OsAUX1 and OsPINs etc., and auxin biosynthesis gene, OsYUCCA5, expressed in various levels compared with control or wild type. Overall, these results concluded that both GSH level and GSH / GSSG ratio influenced auxin biosynthesis, transport and distribution, thereby regulate the development of primary and lateral roots of seminal root in rice seedling. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T07:02:20Z (GMT). No. of bitstreams: 1 ntu-108-R05623018-1.pdf: 4237340 bytes, checksum: ea4c145d14ff365e849a744b18f12b9f (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 口試委員會審定書
致謝 中文摘要…………………………………………………………………………….....i Abstract……………………………………………………………………..………...iii 縮寫字對照表…………………………………………………………………………v 目錄…………………………………………………………………………………..vii 圖目錄…………………………………………………………………………………x 壹、前人文獻…………………………………………………………………………1 一、植物的抗氧化機制…………………………………………………………1 二、植物穀胱甘肽 (Glutathione, GSH) 的生理角色…………………………2 2.1 穀胱甘肽之簡介……………………………………………………….2 2.2 穀胱甘肽之生合成途徑……………………………………………….3 2.3 穀胱甘肽參與細胞週期……………………………………………….3 2.4 穀胱甘肽影響生長發育……………………………………………….3 三、植物穀胱甘肽還原酶 (Glutathione reductase, GR) 的特性與生理功能..5 3.1 GR 的生理構造………………………………………………………..5 3.2 GR 的生理功能………………………………………………………..5 3.3 水稻 GR 基因家族與生理功能……………………………………...6 四、植物生長素 (Auxin) 的生理角色………………………………………...6 4.1 生長素之簡介………………………………………………………….6 4.2 生長素之生合成途徑………………………………………………….7 4.3 生長素之極性運輸…………………………………………………….8 五、水稻根部之發育與調控……………………………………………………9 5.1 植物荷爾蒙交互作用調控根部發育………………………………….9 5.2 水稻主根發育之研究………………………………………………10 5.3 水稻側根發育之研究………………………………………………...11 貳、本論文研究目的及實驗架構…………………………………………………..12 參、材料與方法……………………………………………………………………..13 一、植物材料準備及生長條件………………………………………………..13 二、基因表現分析材料準備及處理…………………………………………..14 三、基因表現分析……………………………………………………………..16 四、水稻試驗處理及生理分析………………………………………………..17 五、根部生長素分佈與累積分析……………………………………………..20 六、統計分析…………………………………………………………………..20 肆、結果……………………………………………………………………………..21 一、GSH 相關物質處理對水稻幼苗種子根生長及生長素分佈之影響……21 二、OsGR1 轉殖株對水稻幼苗種子根生長及生長素分佈之影響…………23 三、穀胱甘肽含量調控水稻種子根內生長素分佈模式圖…………………..24 四、穀胱甘肽氧化還原狀態調節水稻種子根內生長素分佈模式圖………..24 伍、討論……………………………………………………………………………..25 一、穀胱甘肽含量影響水稻種子根生長與發育……………………………..25 二、穀胱甘肽含量改變根部內生長素相關基因表現………………………..26 三、穀胱甘肽含量改變根部內生長素分佈及含量…………………………..28 四、穀胱甘肽氧化還原比例 GSH / GSSG 恆定對水稻根部正常發展的重要性…………………………………………………………………………..29 五、穀胱甘肽氧化還原比例 GSH / GSSG 恆定對水稻根部內生長素相關基因表現的重要性…………………………………………………………..29 六、外加還原型穀胱甘肽 GSH 可促進弱化表現 OsGR1 的側根伸長與生長素分佈…………………………………………………………………..30 七、大量表現 OsGR1 能減緩逆境對生長素分佈與根部生長的抑制……..30 陸、參考文獻………………………………………………………………………..32 柒、附錄……………………………………………………………………………..57 一、水稻生長試驗培養基……………………………………………………..57 二、木村氏水耕液配方………………………………………………………..57 三、變性膠體電泳分析………………………………………………………..58 四、GUS 染色配方……………………………………………………………61 五、本論文基因表現分析使用的引子列表…………………………………..61 | |
| dc.language.iso | zh-TW | |
| dc.subject | 生長素 | zh_TW |
| dc.subject | 穀胱甘? | zh_TW |
| dc.subject | 穀胱甘?還原? | zh_TW |
| dc.subject | 水稻 | zh_TW |
| dc.subject | 根部發育 | zh_TW |
| dc.subject | Glutathione | en |
| dc.subject | glutathione reductase | en |
| dc.subject | root development | en |
| dc.subject | auxin | en |
| dc.subject | rice (Oryza sativa L.) | en |
| dc.title | 水稻穀胱甘肽影響根部發育與生長素極性分布之研究 | zh_TW |
| dc.title | Study the Effect of Glutathione on Rice Root Development and Auxin Polar Distribution | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃文理,張孟基,陸重安,林雅芬 | |
| dc.subject.keyword | 穀胱甘?,穀胱甘?還原?,根部發育,生長素,水稻, | zh_TW |
| dc.subject.keyword | Glutathione,glutathione reductase,root development,auxin,rice (Oryza sativa L.), | en |
| dc.relation.page | 62 | |
| dc.identifier.doi | 10.6342/NTU201902234 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2019-07-31 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農業化學研究所 | zh_TW |
| 顯示於系所單位： | 農業化學系 | |
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