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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻(Tai-Horng Young) | |
dc.contributor.author | Yu-Hsin Wang | en |
dc.contributor.author | 汪郁信 | zh_TW |
dc.date.accessioned | 2021-06-17T07:00:35Z | - |
dc.date.available | 2024-08-07 | |
dc.date.copyright | 2019-08-07 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-01 | |
dc.identifier.citation | [1] R.S. Cargill, 3rd, K.C. Dee, S. Malcolm, An assessment of the strength of NG108-15 cell adhesion to chemically modified surfaces, Biomaterials 20(23-24) (1999) 2417-25.
[2] S. Varon, The culture of chick embryo dorsal root ganglionic cells on polylysine-coated plastic, Neurochemical research 4(2) (1979) 155-73. [3] J.M. Schakenraad, H.J. Busscher, C.R. Wildevuur, J. Arends, The influence of substratum surface free energy on growth and spreading of human fibroblasts in the presence and absence of serum proteins, Journal of biomedical materials research 20(6) (1986) 773-84. [4] K. Smetana, Jr., J. Vacik, D. Souckova, Z. Krcova, J. Sulc, The influence of hydrogel functional groups on cell behavior, Journal of biomedical materials research 24(4) (1990) 463-70. [5] A. Kishida, H. Iwata, Y. Tamada, Y. Ikada, Cell behaviour on polymer surfaces grafted with non-ionic and ionic monomers, Biomaterials 12(8) (1991) 786-92. [6] S.P. Massia, J.A. Hubbell, Immobilized Amines and Basic-Amino-Acids as Mimetic Heparin-Binding Domains for Cell-Surface Proteoglycan-Mediated Adhesion, J Biol Chem 267(14) (1992) 10133-10141. [7] J.H. Lee, H.W. Jung, I.K. Kang, H.B. Lee, Cell behaviour on polymer surfaces with different functional groups, Biomaterials 15(9) (1994) 705-11. [8] E. Yavin, Z. Yavin, Attachment and Culture of Dissociated Cells from Rat Embryo Cerebral Hemispheres on Polylysine-Coated Surface, J Cell Biol 62(2) (1974) 540-546. [9] W.L. Mckeehan, R.G. Ham, Stimulation of Clonal Growth of Normal Fibroblasts with Substrata Coated with Basic Polymers, J Cell Biol 71(3) (1976) 727-734. [10] J.M. Thompson, D.J. Pelto, Attachment, Survival and Neurite Extension of Chick-Embryo Retinal Neurons on Various Culture Substrates, Dev Neurosci-Basel 5(5-6) (1982) 447-457. [11] R. Adler, J. Jerdan, A.T. Hewitt, Responses of Cultured Neural Retinal Cells to Substratum-Bound Laminin and Other Extracellular-Matrix Molecules, Dev Biol 112(1) (1985) 100-114. [12] C.L. Smith, Cytoskeletal Movements and Substrate Interactions during Initiation of Neurite Outgrowth by Sympathetic Neurons in-Vitro, Journal of Neuroscience 14(1) (1994) 384-398. [13] J.H. Ke, M.F. Wei, M.J. Shieh, T.H. Young, Design, Synthesis and Evaluation of Cationic Poly(N-substituent acrylamide)s for In Vitro Gene Delivery, J Biomat Sci-Polym E 22(9) (2011) 1215-1236. [14] G. Levi, F. Aloisi, M.T. Ciotti, V. Gallo, Autoradiographic Localization and Depolarization-Induced Release of Acidic Amino-Acids in Differentiating Cerebellar Granule Cell-Cultures, Brain Res 290(1) (1984) 77-86. [15] T. Mosmann, Rapid Colorimetric Assay for Cellular Growth and Survival - Application to Proliferation and Cyto-Toxicity Assays, J Immunol Methods 65(1-2) (1983) 55-63. [16] L. Esser, C.R. Wang, M. Hosaka, C.S. Smagula, T.C. Sudhof, J. Deisenhofer, Synapsin I is structurally similar to ATP-utilizing enzymes, Embo J 17(4) (1998) 977-984. [17] N.C.f.B. Information, L-lysine | C6H14N2O2 - PubChem, 2004-09-16. https://pubchem.ncbi.nlm.nih.gov/compound/5962. (Accessed July 7 2016). [18] N.C.f.B. Information, n-(4-aminobutyl)acrylamide | C7H14N2O - PubChem, 2007-12-04. https://pubchem.ncbi.nlm.nih.gov/compound/19429107. (Accessed July 10 2016). [19] A. Acheson, J.L. Sunshine, U. Rutishauser, Ncam Polysialic Acid Can Regulate Both Cell Cell and Cell Substrate Interactions, J Cell Biol 114(1) (1991) 143-153. [20] T.W. Rosahl, D. Spillane, M. Missler, J. Herz, D.K. Selig, J.R. Wolff, R.E. Hammer, R.C. Malenka, T.C. Sudhof, Essential functions of synapsins I and II in synaptic vesicle regulation, Nature 375(6531) (1995) 488-93. [21] S. Hilfiker, V.A. Pieribone, A.J. Czernik, H.T. Kao, G.J. Augustine, P. Greengard, Synapsins as regulators of neurotransmitter release, Philosophical transactions of the Royal Society of London. Series B, Biological sciences 354(1381) (1999) 269-79. [22] D. Gitler, Y. Xu, H.T. Kao, D. Lin, S. Lim, J. Feng, P. Greengard, G.J. Augustine, Molecular determinants of synapsin targeting to presynaptic terminals, The Journal of neuroscience : the official journal of the Society for Neuroscience 24(14) (2004) 3711-20. [23] S.H. Song, G.J. Augustine, Synapsin Isoforms and Synaptic Vesicle Trafficking, Molecules and cells 38(11) (2015) 936-40. [24] L.I. Benowitz, A. Routtenberg, GAP-43: an intrinsic determinant of neuronal development and plasticity, Trends in neurosciences 20(2) (1997) 84-91. [25] L.H. Aarts, P. Schotman, J. Verhaagen, L.H. Schrama, W.H. Gispen, The role of the neural growth associated protein B-50/GAP-43 in morphogenesis, Advances in experimental medicine and biology 446 (1998) 85-106. [26] L.F. Eng, Glial fibrillary acidic protein (GFAP): the major protein of glial intermediate filaments in differentiated astrocytes, Journal of neuroimmunology 8(4-6) (1985) 203-14. [27] K.M. Dhandapani, M. Hadman, L. De Sevilla, M.F. Wade, V.B. Mahesh, D.W. Brann, Astrocyte protection of neurons: role of transforming growth factor-beta signaling via a c-Jun-AP-1 protective pathway, J Biol Chem 278(44) (2003) 43329-39. [28] J. Zou, Y.X. Wang, F.F. Dou, H.Z. Lu, Z.W. Ma, P.H. Lu, X.M. Xu, Glutamine synthetase down-regulation reduces astrocyte protection against glutamate excitotoxicity to neurons, Neurochemistry international 56(4) (2010) 577-84. [29] G. Giordano, T.J. Kavanagh, L.G. Costa, Mouse cerebellar astrocytes protect cerebellar granule neurons against toxicity of the polybrominated diphenyl ether (PBDE) mixture DE-71, Neurotoxicology 30(2) (2009) 326-9. [30] G. Barreto, R.E. White, Y. Ouyang, L. Xu, R.G. Giffard, Astrocytes: targets for neuroprotection in stroke, Central nervous system agents in medicinal chemistry 11(2) (2011) 164-73. [31] D.M. Pizzurro, K. Dao, L.G. Costa, Astrocytes protect against diazinon- and diazoxon-induced inhibition of neurite outgrowth by regulating neuronal glutathione, Toxicology 318 (2014) 59-68. [32] Y.B. Ouyang, L. Xu, S. Yue, S. Liu, R.G. Giffard, Neuroprotection by astrocytes in brain ischemia: importance of microRNAs, Neuroscience letters 565 (2014) 53-8. [33] Z. Liu, M. Chopp, Astrocytes, therapeutic targets for neuroprotection and neurorestoration in ischemic stroke, Progress in neurobiology (2015). [34] L.B. Jensen, J. Hjortdal, N. Ehlers, Longterm follow-up of penetrating keratoplasty for keratoconus, Acta ophthalmologica 88(3) (2010) 347-51. [35] N. Al-Yousuf, I. Mavrikakis, E. Mavrikakis, S.M. Daya, Penetrating keratoplasty: indications over a 10 year period, The British Journal of Ophthalmology 88(8) (2004) 998-1001. [36] L. Ham, I. Dapena, C. van Luijk, J. van der Wees, G.R. Melles, Descemet membrane endothelial keratoplasty (DMEK) for Fuchs endothelial dystrophy: review of the first 50 consecutive cases, Eye (London, England) 23(10) (2009) 1990-8. [37] M. Ang, A.M. Dubis, M.R. Wilkins, Descemet membrane endothelial keratoplasty: intraoperative and postoperative imaging spectral-domain optical coherence tomography, Case reports in ophthalmological medicine 2015 (2015) 506251. [38] M.A. Nanavaty, X. Wang, A.J. Shortt, Endothelial keratoplasty versus penetrating keratoplasty for Fuchs endothelial dystrophy, The Cochrane database of systematic reviews (2) (2014) Cd008420. [39] M.A. Terry, Endothelial keratoplasty: a comparison of complication rates and endothelial survival between precut tissue and surgeon-cut tissue by a single DSAEK surgeon, Transactions of the American Ophthalmological Society 107 (2009) 184-91. [40] T.J. Wang, I.J. Wang, F.R. Hu, T.H. Young, Applications of Biomaterials in Corneal Endothelial Tissue Engineering, Cornea 35 Suppl 1 (2016) S25-s30. [41] J.-Y. Lai, G.-H. Hsiue, Functional biomedical polymers for corneal regenerative medicine, Reactive and Functional Polymers 67(11) (2007) 1284-1291. [42] N. Vazquez, C.A. Rodriguez-Barrientos, S.D. Aznar-Cervantes, M. Chacon, J.L. Cenis, A.C. Riestra, R.M. Sanchez-Avila, M. Persinal, A. Brea-Pastor, L. Fernandez-Vega Cueto, A. Meana, J. Merayo-Lloves, Silk Fibroin Films for Corneal Endothelial Regeneration: Transplant in a Rabbit Descemet Membrane Endothelial Keratoplasty, Investigative ophthalmology & visual science 58(9) (2017) 3357-3365. [43] Y.H. Chen, Y.C. Chung, I.J. Wang, T.H. Young, Control of cell attachment on pH-responsive chitosan surface by precise adjustment of medium pH, Biomaterials 33(5) (2012) 1336-42. [44] S.H. Chang, I.N. Chiang, Y.H. Chen, T.H. Young, Serum-free culture of rat proximal tubule cells with enhanced function on chitosan, Acta Biomater 9(11) (2013) 8942-51. [45] A. Sarasam, S.V. Madihally, Characterization of chitosan-polycaprolactone blends for tissue engineering applications, Biomaterials 26(27) (2005) 5500-8. [46] W. Li, A.L. Sabater, Y.T. Chen, Y. Hayashida, S.Y. Chen, H. He, S.C. Tseng, A novel method of isolation, preservation, and expansion of human corneal endothelial cells, Investigative ophthalmology & visual science 48(2) (2007) 614-20. [47] K.M. Crawford, S.A. Ernst, R.F. Meyer, D.K. MacCallum, NaK-ATPase pump sites in cultured bovine corneal endothelium of varying cell density at confluence, Investigative ophthalmology & visual science 36(7) (1995) 1317-26. [48] S.P. Sugrue, J.D. Zieske, ZO1 in corneal epithelium: association to the zonula occludens and adherens junctions, Exp Eye Res 64(1) (1997) 11-20. [49] R.N. Palchesko, K.L. Lathrop, J.L. Funderburgh, A.W. Feinberg, In vitro expansion of corneal endothelial cells on biomimetic substrates, Scientific reports 5 (2015) 7955. [50] Y.H. Wang, S.H. Chang, D. Mau-Hsu, J.H. Wang, T.H. Young, Poly(N-(4-aminobutyl)-acrylamide) as mimetic polylysine for improving survival and differentiation of cerebellar granule neurons, Journal of biomedical materials research. Part B, Applied biomaterials 106(3) (2018) 1194-1201. [51] G.S. Peh, R.W. Beuerman, A. Colman, D.T. Tan, J.S. Mehta, Human corneal endothelial cell expansion for corneal endothelium transplantation: an overview, Transplantation 91(8) (2011) 811-9. [52] M.M. Jumblatt, D.M. Maurice, B.D. Schwartz, A gelatin membrane substrate for the transplantation of tissue cultured cells, Transplantation 29(6) (1980) 498-9. [53] J.P. McCulley, D.M. Maurice, B.D. Schwartz, Corneal endothelial transplantation, Ophthalmology 87(3) (1980) 194-201. [54] T. Mimura, S. Yamagami, S. Yokoo, T. Usui, K. Tanaka, S. Hattori, S. Irie, K. Miyata, M. Araie, S. Amano, Cultured human corneal endothelial cell transplantation with a collagen sheet in a rabbit model, Investigative ophthalmology & visual science 45(9) (2004) 2992-7. [55] T. Sumide, K. Nishida, M. Yamato, T. Ide, Y. Hayashida, K. Watanabe, J. Yang, C. Kohno, A. Kikuchi, N. Maeda, H. Watanabe, T. Okano, Y. Tano, Functional human corneal endothelial cell sheets harvested from temperature-responsive culture surfaces, FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20(2) (2006) 392-4. [56] Y. Liu, H. Sun, M. Hu, M. Zhu, S. Tighe, S. Chen, Y. Zhang, C. Su, S. Cai, P. Guo, Human Corneal Endothelial Cells Expanded In Vitro Are a Powerful Resource for Tissue Engineering, International journal of medical sciences 14(2) (2017) 128-135. [57] S. Ponce Marquez, V.S. Martinez, W. McIntosh Ambrose, J. Wang, N.G. Gantxegui, O. Schein, J. Elisseeff, Decellularization of bovine corneas for tissue engineering applications, Acta Biomater 5(6) (2009) 1839-47. [58] R. Langer, J.P. Vacanti, Tissue engineering, Science (New York, N.Y.) 260(5110) (1993) 920-6. [59] W.M. Bourne, J.W. McLaren, Clinical responses of the corneal endothelium, Exp Eye Res 78(3) (2004) 561-72. [60] J.G. Lee, E.P. Kay, FGF-2-mediated signal transduction during endothelial mesenchymal transformation in corneal endothelial cells, Exp Eye Res 83(6) (2006) 1309-16. [61] S. Piera-Velazquez, Z. Li, S.A. Jimenez, Role of endothelial-mesenchymal transition (EndoMT) in the pathogenesis of fibrotic disorders, The American journal of pathology 179(3) (2011) 1074-80. [62] S. Lamouille, J. Xu, R. Derynck, Molecular mechanisms of epithelial-mesenchymal transition, Nature reviews. Molecular cell biology 15(3) (2014) 178-96. [63] O. Roy, V.B. Leclerc, J.M. Bourget, M. Theriault, S. Proulx, Understanding the process of corneal endothelial morphological change in vitro, Investigative ophthalmology & visual science 56(2) (2015) 1228-37. [64] N. Okumura, R. Minamiyama, L.T. Ho, E.P. Kay, S. Kawasaki, T. Tourtas, U. Schlotzer-Schrehardt, F.E. Kruse, R.D. Young, A.J. Quantock, S. Kinoshita, N. Koizumi, Involvement of ZEB1 and Snail1 in excessive production of extracellular matrix in Fuchs endothelial corneal dystrophy, Laboratory investigation; a journal of technical methods and pathology 95(11) (2015) 1291-304. [65] T. Usui, M. Takase, Y. Kaji, K. Suzuki, K. Ishida, T. Tsuru, K. Miyata, M. Kawabata, H. Yamashita, Extracellular matrix production regulation by TGF-beta in corneal endothelial cells, Investigative ophthalmology & visual science 39(11) (1998) 1981-9. [66] N. Okumura, E.P. Kay, M. Nakahara, J. Hamuro, S. Kinoshita, N. Koizumi, Inhibition of TGF-beta signaling enables human corneal endothelial cell expansion in vitro for use in regenerative medicine, Plos One 8(2) (2013) e58000. [67] T.Y. Kim, W.I. Kim, R.E. Smith, E.D. Kay, Role of p27(Kip1) in cAMP- and TGF-beta2-mediated antiproliferation in rabbit corneal endothelial cells, Investigative ophthalmology & visual science 42(13) (2001) 3142-9. [68] T. Joko, A. Shiraishi, Y. Akune, S. Tokumaru, T. Kobayashi, K. Miyata, Y. Ohashi, Involvement of P38MAPK in human corneal endothelial cell migration induced by TGF-beta(2), Exp Eye Res 108 (2013) 23-32. [69] M. Kimoto, N. Shima, M. Yamaguchi, Y. Hiraoka, S. Amano, S. Yamagami, Development of a bioengineered corneal endothelial cell sheet to fit the corneal curvature, Investigative ophthalmology & visual science 55(4) (2014) 2337-43. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72541 | - |
dc.description.abstract | 人體組織損傷的修補,不可避免的需要靠細胞本身的複製,或是細胞外基質的分泌來達成。因為人體有些細胞in vivo複製緩慢,或是細胞外基質分泌不足,以致光靠自體修復無法完全復原,因此本研究想發展一些材料來改善這些組織的再生能力。
在本研究中,我們以神經細胞及角膜內皮細胞當成研究的重點,嘗試改善現有的生醫材料。在神經的方面,(N-(4-aminobutyl)-acrylamide) (P4Am)是一個正電高分子材料,和目前常用於神經培養的ploy-D-lysine(PDL)有很高的相似度。兩者主要差別是在胜肽鍵的結構,前者放在側鏈上,後者則是在主鏈上。我們假設神經細胞有足夠的敏感性可以分辨兩種材料的不同,所以我們用7天大的wistar大鼠小腦顆粒神經細胞,培養在將材料序列稀釋並且塗佈的培養基材上。在低濃度的塗佈條件下(< 0.16 µg/ml),從細胞活性和型態分析可以發現神經細胞能夠區分兩種材料的結構差異。P4Am相比起PDL即使在低濃度下也能讓每個神經球結點保留平均超過3條神經束,維持神經網路的結構。這表示低濃度的P4Am胜肽鍵結構在和神經的交互作用有助於神經存活分化。 雖然成熟的神經細胞無法分裂複製,但新材料有助於神經存活和網路結構連結。對於嚴重的神經損傷,如何引導神經纖維的生長和維持結構是必要的。P4Am在神經組織工程上可當成新的生物基材。 在角膜內皮方面,我們目標放在分析chitosan/PCL混摻材料上的角膜內皮分化的機制和分析細胞外基質(ECM)成分,發現其用於組織工程的潛力。牛角膜細胞培養於此混摻材料上的貼附、複製、分化以及蛋白表現會和TCPS以及純chitosan作比較。此外我們還會特別分析幾種ECM的結果。從AFM發現PCL25比起純chitosan要粗糙,FTIR也證實了C=O官能基確實有進入膜材中。角膜內皮細胞在PCL25上面的型態和正常TCPS上都呈現一致的多角形以及相近的生長速度。此外在PCL25上的ECM分泌並沒有比TCPS要差,尤其collagen-IV表現更高,而TGFβ2表現則較低。 雖然神經系統和角膜內皮的損傷在人體很難完全復原,我們的研究找到具有發展潛力的材料,以及在組織工程應用上新的分析標的。 | zh_TW |
dc.description.abstract | Cell proliferation or extracellular matrix secretion is necessary for human tissues. Due to slow proliferating properties of cells in vivo, or insufficient extracellular secreting, some tissues cannot automatically repair after damage. Thus, we want to develop some biomaterials to improve the repairability of those tissues.
In this study, we focused on neurons and corneal endothelial cells and tried to improve existing biomaterials for tissue engineering. For neurons, (N-(4-aminobutyl)-acrylamide) (P4Am) is a positively charged material similar to well-known ploy-D-lysine(PDL), which is regularly used for neuron culture. The main difference is the peptide structure, which is in the backbone of PDL but locating at the side chain of P4Am. We assumed that neurons are sensitive enough to distinguish such structure difference, so these two cationic polymers were compared at serial coating concentrations for culturing cerebellar granule neurons from 7-day-old Wistar rats in this study. Cellular viability and morphology assay in the peptide structure between P4Am and PDL could be distinguished by neurons at low coating concentrations (< 0.16 µg/ml). P4Am at low coating concentration could keep aggregates with three or four thick processes to support more complete neural network with higher cellular viability than PDL. This suggests that the interaction between neurons and the specific peptide structure of P4Am at low coating concentration was able to improve survival and differentiation of cultured cerebellar granule neurons. Although mature neurons cannot proliferate, new material increased cell viability and network connection. For severe neural damage, guiding neurite outgrowth and maintaining networks are essential. Therefore P4Am is a potential candidate for neural tissue engineering. For cornea, we aimed to investigate the underlying mechanisms of the differentiation corneal endothelial cells (CECs) and to identify the compositions of extracellular matrix (ECM) using a chitosan/ polycaprolactone (PCL) blended membrane to explore the potential use of chitosan/PCL blends in tissue engineering of CECs. Bovine CECs were cultured on the blends and compared with TCPS and pure chitosan membrane. Cell behaviors in terms of cell attachment, proliferation, differentiation phenotype and expression of differentiation proteins were examined. Furthermore, the production of ECM proteins was also analyzed. Through experiments, we found that the topography (roughness) of PCL25 membrane examined by AFM was greater than that of pure chitosan membrane. FTIR results confirmed the C=O groups of PCL. The CECs displayed hexagonal morphology and a similar proliferation rate on both PCL25 membrane and TCPS. The production of ECM protein productions of CECs on PCL was not inferior to TCPS. Moreover, Western blot results verified the higher amount of collagen IV, and lower TGF-β2 expression on PCL25 membrane than that on TCPS substrate. Although the nervous system and corneal endothelial cells hardly completely recover in the human body, our studies found not only a potential material but also new indicators to qualify our materials for tissue engineering. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T07:00:35Z (GMT). No. of bitstreams: 1 ntu-108-D01548012-1.pdf: 3367422 bytes, checksum: 1bc91d5917441051c8260495cdbd7c2d (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 iii ABSTRACT v Contents vii Figures x Chapter 1. BACKGROUND 1 Chapter 2. Poly (N-(4-aminobutyl)-acrylamide) as mimetic polylysine for improving survival and differentiation of cerebellar granule neurons 4 2.1 Introduction 4 2.2 Materials and methods 6 2.2.1 Preparation of culture substrates 6 2.2.2 Cell culture 6 2.2.3 Cell viability MTT assay 7 2.2.4 Immunocytochemical staining and characterization 7 2.2.5 Cell viability alamar blue assay 8 2.2.6 Statistical analysis 8 2.3 Results 9 2.3.1 Cellular viability 9 2.3.2 Cell morphology 11 2.3.3 Cytotoxicity 17 2.3.4 Neurotropic protein expression 19 2.3.5 Glia cells viability on serial PDL-coating materials 24 2.4 Discussion 24 Chapter 3. Investigating the Effect of Chitosan/ Polycaprolactone Blends in Differentiation of Corneal Endothelial Cells and Extracellular Matrix Compositions. 30 3.1 Introduction 30 3.2 Materials and Methods 31 3.2.1 Preparation of culture substrates 31 3.2.2 Preparation and characterization of PCL25 blending material 31 3.2.3 Isolation and culture of bovine CECs 32 3.2.4 Cell proliferation assay (MTT, WST-1) 33 3.2.5 Cell attachment ratio test 34 3.2.6 Immunofluorescence assay 34 3.2.7 Preparation of total protein lysate 34 3.2.8 Western blot analysis 35 3.2.9 Statistical analysis 35 3.3 Results 36 3.3.1 PDL-coating for corneal endothelial cells viability 36 3.3.2 Fundamental structural characteristics of chitosan and PCL25 blended membranes 38 3.3.3 The morphology, attachment ratio and proliferation ability of CECs on chitosan, PCL25 blended membrane and TCPS 40 3.3.4 Immunofluorescence staining of CECs on PCL25 membrane and TCPS 44 3.3.5 Western blot analysis 46 3.4 Discussion 48 Chapter 4. Conclusions 51 Chapter 5. 附錄 53 Chapter 6. References 54 | |
dc.language.iso | en | |
dc.title | 用於人體不易生長組織的生醫材料之研發 | zh_TW |
dc.title | Development of innovative biomaterials for slow proliferating human tissues | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 王至弘(Jyh-Horng Wang),楊銘乾(Ming-Chien Yang),林宏殷(Hung-Yin Lin),李玫樺(Mei-Hua Lee) | |
dc.subject.keyword | 神經,角膜內皮,複製,組織修補,細胞外基質, | zh_TW |
dc.subject.keyword | neuron,corneal endothelial cells,proliferation,tissue recovery,extracellular matrix, | en |
dc.relation.page | 58 | |
dc.identifier.doi | 10.6342/NTU201902344 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-02 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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