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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 嚴震東(Chen-Tung Yen) | |
| dc.contributor.author | Ya-Wen Wu | en |
| dc.contributor.author | 吳雅雯 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:40:41Z | - |
| dc.date.available | 2028-08-15 | |
| dc.date.copyright | 2018-08-18 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-08-15 | |
| dc.identifier.citation | References
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72408 | - |
| dc.description.abstract | 神經性疼痛是由感覺神經系統損傷引起的。在本研究中，我們使用顯微鏡方法來檢查坐骨神經的慢性壓迫損傷（CCI）後的皮膚下的傷害感受器的變化。我們使用標記螢光纖維Nav1.8-tdTomato的基因轉殖小鼠，並直接利用顯微觀察神經末梢的疼痛感受器並觀察神經性疼痛的發展變化過程。行為測試包括Von-Frey刺激，輻射熱板測試，分別測試其對機械和熱刺激的敏感性。在CCI手術前及3〜21天後，利用共聚焦顯微鏡和雙光子螢光顯微鏡觀察後腳掌神經叢和游離神經末梢的動態變化。我們初步的數據顯示神經纖維的數量與痛覺敏感程度並不一致。利用ATF3檢驗老鼠背根神經細胞受損的程度發現在不同腳掌神經損害組別間沒有顯著差異。由此推論證明受傷後早期的腳掌神經末梢的密度可能是CCI神經病變嚴重程度的有用生物標記。 | zh_TW |
| dc.description.abstract | Neuropathic pain is caused by injury of the somatosensory nervous system. In this study we aim to use in vivo and ex vivo microscopic methods to examine the change in nociceptors under the paw skin after chronic constriction injury (CCI) of the sciatic nerve, a commonly used animal model for neuropathic pain research. Our basic hypothesis is that direct microscopic observation of free nerve endings of the nociceptors in situ and en bloc will reveal the life history of the development and maintenance of the neuropathic pain. We used genetically modified mice that intrinsically express the fluorophore tdTomato tagged to a small fiber marker, the Nav1.8 molecule. A battery of behavior tests were applied to the hind paws of the mice to test their sensitivity to mechanical and heat stimuli, respectively. Confocal microscopic and longitudinal two-photon fluorescent microscopic observation of dynamic changes of nerve plexus and free nerve endings in the hind paw were performed first before and then 2 to 21 days after CCI of the sciatic nerve. Using behavioral tests we validated mechanical and thermal hyperalgesia of the CCI mice. Our preliminary data revealed a mismatch of the severity of mechanical hypersensitivity versus the extent of fibers degeneration. Use ATF3-IHC we found that there was no significant different of nerve injured between mild and severe groups. Thus the density of the Nav1.8-tdTomato nerve ending in the hind paw at early injury stage can be a useful biomarker for the severity of the CCI induced neuropathy. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:40:41Z (GMT). No. of bitstreams: 1 ntu-107-R05b21005-1.pdf: 7504145 bytes, checksum: 2e112b3f906731d73f1785df751bd16f (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 中文摘要 ii
ABSTRACT iii CONTENTS iv LIST OF FIGURES vi Introduction 1 1.1 Skin innervation 1 1.2 Nociceptor 2 1.3 Nav1.8 3 1.4 Neuropathic pain 4 1.5 Neuropathic pain induced by chronic construction injury (CCI) 5 1.6 Confocal and Two-photon fluorescent microscopy 8 1.7 Aim of this study 10 Materials and Methods 12 2.1 Animals and Surgery 12 2.2 Behavioral tests 13 2.2.1 Von-Frey Sensory Testing 13 2.2.2 Measurement of Thermal Sensitivity 14 2.3 In vivo Two-Photon fluorescence light microscopy 14 2.4 Immunohistochemistry 17 2.5 Statistical analysis 18 Results 20 3.1 Mice developed mechanical hypersensitivity and thermal hyperalgesia after CCI 20 3.2 In vivo longitudinal changes of small fibers in the hind paw 21 3.3 CCI-induced neuropathic pain and the progressive loss of skin innervation 22 3.4 Longitudinal in vivo image of degeneration and sprouting of nerve fibers in the 5th toe 23 3.5 AFT3 immunochemistry 24 Discussion. 25 4.1 Neuropathic pain behavior 25 4.2 Using microscopic observations the role of nerve injury in generation of neuropathic pain 26 4.3 In vivo two-photon imaging 28 4.4 Conclusion 29 4.5 Significance and future works 30 References 32 | |
| dc.language.iso | en | |
| dc.subject | 雙光子螢光顯微鏡 | zh_TW |
| dc.subject | 神經病變性疼痛 | zh_TW |
| dc.subject | 痛感受器 | zh_TW |
| dc.subject | 慢性神經壓迫 | zh_TW |
| dc.subject | chronic constriction injury | en |
| dc.subject | two-photon fluorescent microscopy | en |
| dc.subject | Neuropathic pain | en |
| dc.subject | Nociceptor | en |
| dc.title | 以光學方式觀察慢性壓迫神經小鼠痛覺神經的變化 | zh_TW |
| dc.title | Visualize nociceptor changes in the hind paw of neuropathic mice with chronic constriction injury | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 謝松蒼(Sung-Tsang Hsieh),閔明源(Ming-Yuan Min),陳志成(Chih-Cheng Chen) | |
| dc.subject.keyword | 神經病變性疼痛,痛感受器,慢性神經壓迫,雙光子螢光顯微鏡, | zh_TW |
| dc.subject.keyword | Neuropathic pain,Nociceptor,chronic constriction injury,two-photon fluorescent microscopy, | en |
| dc.relation.page | 60 | |
| dc.identifier.doi | 10.6342/NTU201802114 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-08-16 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| 顯示於系所單位： | 生命科學系 | |
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