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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 張英?(Ing-Feng Chang) | |
dc.contributor.author | Cheng-Che Yeh | en |
dc.contributor.author | 葉承哲 | zh_TW |
dc.date.accessioned | 2021-06-17T06:36:52Z | - |
dc.date.available | 2020-08-21 | |
dc.date.copyright | 2018-08-21 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-15 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72348 | - |
dc.description.abstract | 氯化鈉是廣泛存在於整個地球的鹽類,大多數植物不像動物需要攝取鈉離子,鈉離子容易對植物產生毒性,造成鹽逆境。比起鈉鹽的廣泛存在和對植物影響的廣大研究,另一個鹼金族金屬鋰形成的鋰鹽,氯化鋰,對植物的影響相對較少被研究。已知氯化鋰影響植物乙烯生合成相關基因1-aminocyclopropane-1-carboxylase synthase (ACS) 的表現,而乙烯也參與植物對抗鹽逆境,其中也包括離層酸(ABA)的參與,然而鋰鹽對於ACSs的表現影響並不完全清楚。本研究發現氯化鋰處理下,氯化鈉抑制阿拉伯芥種子發芽率會明顯恢復被抑制的發芽率,且更加降低氯化鈉逆境下的幼苗存活率。另外,本研究試著從基因表現層面解釋氯化鋰影響植物抵抗非生物性逆境和向地性差異的影響。基因表現的結果顯示加了氯化鋰和重力處理下ACSs和ABA相關基因表現有差異。 | zh_TW |
dc.description.abstract | Sodium chloride (NaCl) causes salt stress to plant world wide.Unlike NaCl is widely studied in plant, lithium chloride (LiCl), the salt composed from another alkali metal lithium. LiCl has been known to influence the gene expression of plant ethylene biosynthesis 1-aminocyclopropane-1-carboxylase synthase (ACS) enzyme. However, the influence of lithium salt to gene expression of ACSs is still not been further linked to ABA and NaCl with ethylene. The seed germination rate inhibited by NaCl stress was recovered by LiCl treatment, but the toxicity from NaCl stress for seedling survival was enhanced by LiCl. The data from gene expression indicates differential expression of ACSs and ABA-related genes by LiCl and gravity treatment. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T06:36:52Z (GMT). No. of bitstreams: 1 ntu-107-R03b42028-1.pdf: 2061331 bytes, checksum: 6b766f3f0fde7b0eee3ee9baedd477b7 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 目錄
摘要 V Abstract VI 前言 1 鹽逆境反應 1 離層酸abscisic acid (ABA) 2 鋰鹽 2 種子發芽 3 根部向地性 3 乙烯生合成 4 研究目標 5 材料與方法 6 植物材料和生長條件 6 種子發芽率觀察 6 植物向地性觀察 6 鹽逆境及ABA處理 7 基因表現量分析 7 結果 9 植物向地性觀察 9 向地性處理與氯化鋰處理對於乙烯生合成相關基因ACSs和非生物性逆境基因表現的影響 9 氯化鋰和氯化鈉共同影響幼苗存活率 10 氯化鋰和氯化鈉共同處理影響種子發芽率的程度有別 10 氯化鋰、氯化鈉鹽逆境及離層酸處理下以即時定量PCR檢測鹽逆境相關基因之表現 10 討論 12 氯化鋰影響鹽逆境反應 12 氯化鋰、氯化鈉鹽逆境及離層酸處理下以即時定量PCR檢測逆境相關基因之表現 12 向地性處理與氯化鋰處理對於ACSs基因表現的影響 13 結語 13 參考文獻 14 附錄--- T-DNA插入突變株atacs7-1 之研究 34 T-DNA插入突變株之篩選 34 確認atacs7-1 突變株之T-DNA插入位點 34 向地性觀察 34 向地性處理與氯化鋰處理對於乙烯生合成相關基因ACSs和非生物性逆境基因表現的影響 35 氯化鋰和氯化鈉共同影響幼苗存活率和發芽率 35 氯化鋰、氯化鈉鹽逆境及離層酸處理下以即時定量PCR檢測鹽逆境相關基因之表現 36 T-DNA突變株acs7-1 表現之疑慮 37 附表 54 附件 58 圖目錄 圖一 20 圖二 27 圖三 28 圖四 29 圖五 33 附圖一 38 附圖二 40 附圖三 47 附圖四 48 附圖五 49 附圖六 53 | |
dc.language.iso | zh-TW | |
dc.title | 氯化鋰對阿拉伯芥向地性及種子萌芽之影響 | zh_TW |
dc.title | Effects of LiCl on root gravitropism and seed germination in Arabidopsis thaliana | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 謝旭亮(Hsu-Liang Hsieh),鄭秋萍(Chiu-Ping Cheng),張孟基(Men-Chi Chang) | |
dc.subject.keyword | 氯化鈉,氯化鋰,離層酸,鹽逆境,向地性, | zh_TW |
dc.subject.keyword | NaCl,LiCl,abscisic acid,salt stress,gravitropism, | en |
dc.relation.page | 63 | |
dc.identifier.doi | 10.6342/NTU201803712 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-16 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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