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標題: | 水稻細胞分裂素訊息反應調節蛋白 OsRR6 與 OsRR11 於鹽害逆境下之生理功能探討 Functional Characterization of Rice Cytokinin Response regulators OsRR6 and OsRR11 in Salinity Stress |
作者: | Han-Yi Wu 吳函䭲 |
指導教授: | 蔡育彰(Yu-Chang Tsai) |
關鍵字: | 水稻,細胞分裂素,訊息傳導,OsRR6,OsRR11,鹽害,轉錄體分析, Oryza sativa,cytokinin,signaling,OsRR6,OsRR11,salt stress,RNA-seq, |
出版年 : | 2018 |
學位: | 碩士 |
摘要: | 隨著全球氣候變遷與全球暖化之影響,作物在非生物逆境下如乾旱、鹽害、冷害及熱逆境下的生長受到阻礙使產量下降,因此了解作物在非生物逆境下之反應機制及耐受性之相關研究亦趨重要。植物荷爾蒙調控著作物的生長發育,同時亦在非生物逆境下之反應機制扮演重要的角色,其中細胞分裂素之生合成及運輸於非生物逆境下皆會明顯地受到抑制,被認為是非生物逆境下之負向調控因子。細胞分裂素之反應是藉由雙分子訊息傳導系統 (two-component signaling system, TCS) 進行調控,其中在前人研究中顯示Type-A RRs能快速地受細胞分裂素誘導,被認為是細胞分裂素之原初反應因子,且在細胞分裂素訊息傳導中扮演負向調控之角色。水稻中Type-A OsRRs共有13個,其中OsRR6在前人研究中發現其基因表現量會明顯受到非生物逆境之誘導,考慮此基因家族間可能有基因功能之補償性,本試驗利用CRISPR/Cas9基因編輯之Osrr6/11突變株進行鹽害下OsRR6及OsRR11之生理功能探討。Osrr6/11突變株於鹽害逆境下有較野生型敏感之外表型,且相較於野生型有較多之鈉離子及過氧化氫累積之現象,其細胞膜穩定性及抗氧化酵素活性亦較野生型差,顯示OsRR6及OsRR11可能在鹽害逆境下參與地上部鈉離子之運輸及抗氧化逆境相關之機制調控中,並且在耐受性機制上扮演正向調控之角色。使用轉錄體定序 (RNA-seq) 進行全基因體表現分析發現OsRR6及OsRR11可能參與在細胞分裂素之活性調控、地上部鈉離子之運輸及與離層酸之生合成及訊息傳導之交互作用中。本試驗結果顯示OsRR6及OsRR11於鹽害逆境下,參與在離子平衡、抗氧化機制、細胞分裂素活性以及離層酸生合成與訊息反應,並扮演鹽害耐受性之正向調控因子。 As the global warming and climate change is getting worse, abiotic stress such as drought, salinity, cold and heat stress usually limit growth development of crops and even reduce yield production. Therefore, understanding the response and the mechanism of crops under abiotic stress becomes more important. Plant hormones are key regulators of plant growth and development. Besides, they also play important roles in the response of abiotic stress in plants. Among them, the production and transportation of cytokinin, which is considered as a negative regulator of abiotic stress, is repressed after the stress treatments in previous studies. The responses of cytokinin are mediate by two-component signaling pathway. In cytokinin signaling pathway, the expression of type-A RR genes are rapidly induced by exogenous cytokinin and hence considered as cytokinin primary response genes. Some of type-A RRs in rice act as negative regulators in cytokinin signaling pathway. It has been known that there are 13 type-A OsRRs in rice and the gene expression of OsRR6 is significantly induced under several abiotic stress. Considering there may have redundant functions in type-A OsRRs, we use CRISPR/Cas9 genome editing Osrr6/11 mutants to study the physiological functions of OsRR6 and OsRR11 in salinity stress. In this study, Osrr6/11 double mutants had salt-sensitive phenotype, poor membrane stability, antioxidant enzyme activities and accumulated more sodium in shoot under salt stress. In addition, whole-genome gene expression profiling analysis showed that OsRR6 and OsRR11 may involve in cytokinin conjugation, sodium transportation and abscisic acid biosynthesis and signaling crosstalk in salt stress. These results show that OsRR6 and OsRR11 may be positive regulators in the response of salt stress in rice. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72332 |
DOI: | 10.6342/NTU201802459 |
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顯示於系所單位: | 農藝學系 |
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