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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 趙福杉(Fu-shan Jaw) | |
| dc.contributor.author | Wei-Zhi Li | en |
| dc.contributor.author | 李韋志 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:35:08Z | - |
| dc.date.available | 2018-08-21 | |
| dc.date.copyright | 2018-08-21 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-08-16 | |
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[2] J. Park, J.T. Decker, D.J. Margul, D.R. Smith, B.J. Cummings, A.J. Anderson, L.D. Shea, Local Immunomodulation with Anti-inflammatory Cytokine-Encoding Lentivirus Enhances Functional Recovery after Spinal Cord Injury, Mol Ther, 26 (2018) 1756-1770. [3] S. Almutiri, M. Berry, A. Logan, Z. Ahmed, Non-viral-mediated suppression of AMIGO3 promotes disinhibited NT3-mediated regeneration of spinal cord dorsal column axons, Sci Rep, 8 (2018) 10707. [4] M. Shahrezaie, R.N. Mansour, B. Nazari, H. Hassannia, F. Hosseini, H. Mahboudi, M. Eftekhary, M. Kehtari, A. Veshkini, A. Ahmadi Vasmehjani, S.E. Enderami, Improved stem cell therapy of spinal cord injury using GDNF-overexpressed bone marrow stem cells in a rat model, Biologicals, 50 (2017) 73-80. [5] A.M. Delaney, C.F. Adams, A.R. Fernandes, A.F. Al-Shakli, J. Sen, D.R. Carwardine, N. Granger, D.M. Chari, A fusion of minicircle DNA and nanoparticle delivery technologies facilitates therapeutic genetic engineering of autologous canine olfactory mucosal cells, Nanoscale, 9 (2017) 8560-8566. [6] Y.M. Park, S.H. Han, S.K. Seo, K.A. Park, W.T. Lee, J.E. Lee, Restorative benefits of transplanting human mesenchymal stromal cells overexpressing arginine decarboxylase genes after spinal cord injury, Cytotherapy, 17 (2015) 25-37. [7] B. Xu, D. Park, Y. Ohtake, H. Li, U. Hayat, J. Liu, M.E. Selzer, F.M. Longo, S. Li, Role of CSPG receptor LAR phosphatase in restricting axon regeneration after CNS injury, Neurobiol Dis, 73 (2015) 36-48. [8] Q.E. Ali, L. Manjunatha, S.H. Amir, S. Jamil, A. Quadir, Efficacy of clonidine as an adjuvant to ropivacaine in supraclavicular brachial plexus block: A prospective study, Indian J Anaesth, 58 (2014) 709-713. [9] C. Xu, M.C. Klaw, M.A. Lemay, P.W. Baas, V.J. Tom, Pharmacologically inhibiting kinesin-5 activity with monastrol promotes axonal regeneration following spinal cord injury, Exp Neurol, 263 (2015) 172-176. [10] M.A. Anderson, J.E. Burda, Y. Ren, Y. Ao, T.M. O'Shea, R. Kawaguchi, G. Coppola, B.S. Khakh, T.J. Deming, M.V. Sofroniew, Astrocyte scar formation aids central nervous system axon regeneration, Nature, 532 (2016) 195-200. [11] B.J. Kerr, P.H. Patterson, Leukemia inhibitory factor promotes oligodendrocyte survival after spinal cord injury, Glia, 51 (2005) 73-79. [12] N.B. Hamilton, L.E. Clarke, I.L. Arancibia-Carcamo, E. Kougioumtzidou, M. Matthey, R. Karadottir, L. Whiteley, L.H. Bergersen, W.D. Richardson, D. Attwell, Endogenous GABA controls oligodendrocyte lineage cell number, myelination, and CNS internode length, Glia, 65 (2017) 309-321. [13] X. Gu, F. Ding, D.F. Williams, Neural tissue engineering options for peripheral nerve regeneration, Biomaterials, 35 (2014) 6143-6156. [14] K. Shibuya, S. Misawa, S. Nasu, Y. Sekiguchi, M. Beppu, Y. Iwai, S. Mitsuma, S. Isose, K. Arimura, R. Kaji, S. Kuwabara, Safety and efficacy of intravenous ultra-high dose methylcobalamin treatment for peripheral neuropathy: a phase I/II open label clinical trial, Intern Med, 53 (2014) 1927-1931. [15] K. Okada, H. Tanaka, K. Temporin, M. Okamoto, Y. Kuroda, H. Moritomo, T. Murase, H. Yoshikawa, Methylcobalamin increases Erk1/2 and Akt activities through the methylation cycle and promotes nerve regeneration in a rat sciatic nerve injury model, Exp Neurol, 222 (2010) 191-203. [16] S. Nishimoto, H. Tanaka, M. Okamoto, K. Okada, T. Murase, H. Yoshikawa, Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerve, Front Cell Neurosci, 9 (2015) 298. [17] F.S. Jaw, Analysis of compound action potential and field potential of the nervous system, and development of a recording system., DOI (1992). [18] S.Z. Yang, Exploring thalamocortical connection by utilizing deep-brain stimulation., DOI (2017). [19] K. Makowiecki, A. Garrett, V. Clark, S.L. Graham, J. Rodger, Reliability of VEP Recordings Using Chronically Implanted Screw Electrodes in Mice, Transl Vis Sci Technol, 4 (2015) 15. [20] Y.K. Hirshler, U. Polat, A. Biegon, Intracranial electrode implantation produces regional neuroinflammation and memory deficits in rats, Exp Neurol, 222 (2010) 42-50. [21] G. Buzsaki, C.A. Anastassiou, C. Koch, The origin of extracellular fields and currents--EEG, ECoG, LFP and spikes, Nat Rev Neurosci, 13 (2012) 407-420. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72318 | - |
| dc.description.abstract | 脊髓損傷造成的癱瘓對於患者是一輩子的折磨，至今尚未有一個標準的方法可以有效地治療。許多人嘗試注射各種藥物，且使用各種組織染色方式以觀察變化，企圖達到更好的進展，但恢復效果仍十分有限。由於注射維他命B12運用在周邊神經具有恢復神經生長的療效，因此本論文以誘發之電生理反應為依據，輔以組織染色切片，藉以觀察維他命B12對於脊髓損傷的治療作用。
為了達到此目的，我們設計於T8至T9之脊椎高度傷害大鼠單側背索（dorsal funiculus）做為形成脊髓損傷之模型，並設計了一簡單之雙通道微電極，以長期量測視丘內神經元之反應。這些實驗大鼠在存活一至三個月後犧牲，並取背索之電顯厚片在甲基藍染色後觀察脊髓背索面積和軸突數量的變化。 實驗結果顯示，在電生理訊號方面，有注射維他命B12之大鼠在傷害後第一週至第二週內有回升的現象。另外，在組織切片部分，長達三個月的時間下，沒有注射維他命B12之大鼠的背索面積，比起有注射維他命B12之大鼠還要少很多。從上述兩個面向的觀察中，得知維他命B12對於受傷之脊髓具有延緩退化甚至修復之高度可能性。 | zh_TW |
| dc.description.abstract | Paralysis caused by spinal cord injury (SCI) is a torture for patients in a life span. There is no way to cure paralysis nowaday. Many people managed to make a progress upon this through injecting various medicine. However, the recovery is still limited. Therefore, this study attempted to observe the effect of vitamin B12 on SCI rats by electrophysiological recordings, and subsidiarily supported by histological examinations.
For chronic recordings, 2-channel micro-wire electrode was embedded to the scalp of the rat to record the evoked neuronal responses reaction of neurons in thalamus. Then, one side of the dorsal column of the rat spinal cord between the T8 and T9 segment was lesioned to induce the SCI model. The results showed that the signals of rats injected with vitamin B12 bounced back from week 1 to week 2 after SCI. Morphologically, after three months the areas of dorsal funiculus of non-treated rats were much less than those of rats which were treated with B12. Based on the preliminary results, vitamin B12 might have the high possibility of degeneration retarding or even recovery to SCI. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:35:08Z (GMT). No. of bitstreams: 1 ntu-107-R05548015-1.pdf: 9760490 bytes, checksum: 7884cbf2b6a6f4184fc7cc1614096951 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 口試委員會審定書............................................................................i
誌謝................................................................................................ii 中文摘要........................................................................................iii Abstract........................................................................................iv 目錄................................................................................................v 圖目錄...........................................................................................vii 第一章、緒論..................................................................................1 1.1 前言...........................................................................................1 1.2 研究動機與目的.........................................................................3 第二章、研究方法與架構.................................................................4 2.1 Micro-wire微電極製作...............................................................5 2.2 動物準備...................................................................................6 2.3 周邊刺激...................................................................................7 2.4 電極包埋...................................................................................7 2.5 中樞神經損傷............................................................................7 2.6 記錄系統...................................................................................8 2.7 訊號分析...................................................................................9 2.8 染色切片...................................................................................9 2.9 脊髓之染色切片分析.................................................................11 第三章、結果................................................................................24 3.1 大鼠視丘VPL之定位.................................................................24 3.2 固定之脊髓傷害位置................................................................24 3.3 長期神經訊號量測分析.............................................................24 3.4 脊髓之染色切片分析................................................................25 第四章、討論................................................................................33 4.1 微電極之持久性.......................................................................33 4.2 注射維他命B12之影響..............................................................33 參考資料.......................................................................................36 | |
| dc.language.iso | zh-TW | |
| dc.subject | 不完全脊髓損傷 | zh_TW |
| dc.subject | 維他命B12 | zh_TW |
| dc.subject | 微電極 | zh_TW |
| dc.subject | 長期訊號量測 | zh_TW |
| dc.subject | 甲基藍染色 | zh_TW |
| dc.subject | Vitamin B12 | en |
| dc.subject | Micro-wire electrode | en |
| dc.subject | Long-term recording | en |
| dc.subject | Spinal cord injury | en |
| dc.subject | Toluidine stain | en |
| dc.title | 大鼠脊髓損傷之中樞神經再生之早期評估----以維他命B12作為急性受術之填充生長因子 | zh_TW |
| dc.title | Early evaluation of central nervous regeneration in rat spinal cord injury model with vitamin B12 as a growth factor during the surgery of acute stage | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 陳淑華(Seu-Hwa Chen) | |
| dc.contributor.oralexamcommittee | 高瑀絜,陳芝萍 | |
| dc.subject.keyword | 維他命B12,微電極,長期訊號量測,不完全脊髓損傷,甲基藍染色, | zh_TW |
| dc.subject.keyword | Vitamin B12,Micro-wire electrode,Long-term recording,Spinal cord injury,Toluidine stain, | en |
| dc.relation.page | 38 | |
| dc.identifier.doi | 10.6342/NTU201803439 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-08-16 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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