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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72065完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳力騏 | |
| dc.contributor.author | Yen Lin | en |
| dc.contributor.author | 林延 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:21:35Z | - |
| dc.date.available | 2019-08-21 | |
| dc.date.copyright | 2018-08-21 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-08-18 | |
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Second order advantage in the determination of amaranth, sunset yellow FCF and tartrazine by UV–vis and multivariate curve resolution-alternating least squares.Analytica chimica acta,655(1-2), 38-42. Martinou, A., Kafetzopoulos, D., & Bouriotis, V. 1995. Chitin deacetylation by enzymatic means: monitoring of deacetylation processes.Carbohydrate Research,273(2), 235-242. Methacanon, P., Prasitsilp, M., Pothsree, T., & Pattaraarchachai, J. 2003. Heterogeneous N-deacetylation of squid chitin in alkaline solution. Carbohydrate Polymers, 52(2), 119-123. Momenzadeh, H., Tehrani-Bagha, A. R., Khosravi, A., Gharanjig, K., & Holmberg, K. 2011. Reactive dye removal from wastewater using a chitosan nanodispersion.Desalination,271(1-3), 225-230. Moutinho, I. L. D., Bertges, L. C., & Assis, R. V. C. 2007. Prolonged use of the food dye tartrazine (FD&C yellow n° 5) and its effects on the gastric mucosa of Wistar rats.Brazilian journal of biology,67(1), 141-145. 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Capacity of adsorption of Pb2+ and Ni2+ from aqueous solutions by chitosan produced from silkworm chrysalides in different degrees of deacetylation.Journal of hazardous materials,147(1-2), 139-147. Pillai, C. K. S., Paul, W., & Sharma, C. P. 2009. Chitin and chitosan polymers: Chemistry, solubility and fiber formation. Progress in polymer science,34(7), 641-678. Potivas, T., & Laokuldilok, T. 2014. Deacetylation of chitin and the properties of chitosan films with various deacetylation degrees.CMUJ NS Special Issue on Food and Applied Bioscience,13(1), 559-567. Raymond, L., Morin, F. G., & Marchessault, R. H. 1993. Degree of deacetylation of chitosan using conductometric titration and solid-state NMR. Carbohydrate Research,246(1), 331-336. Rinaudo, M. 2006. Chitin and chitosan: properties and applications. Progress in polymer science,31(7), 603-632. Rovina, K., Prabakaran, P. P., Siddiquee, S., & Shaarani, S. M. 2016. 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A review on chitin and chitosan polymers:structure,chemistry,solubility,derivatives,and,applications.ChemBioEng Reviews, 2(3), 204-226 Zhang, H., Zhao, X., Liu, S., Yang, J., Duan, R., Yuan, Y., & Hu, X. 2017. Determination of Sunset Yellow Based on Its Quenching Effect on the Fluorescence of Acridine Orange. Analytical Sciences,33(2), 243-247. Zheng, L. Y., & Zhu, J. F. 2003. Study on antimicrobial activity of chitosan with different molecular weights. Carbohydrate polymers, 54(4), 527-530. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72065 | - |
| dc.description.abstract | 幾丁聚醣（Chitosan）是近年來重要的生物性材料，其中去乙醯率（Degree of deacetylation, DD%）的程度高低對於幾丁聚醣的生物性質有顯著的影響。在過去檢測去乙醯率的公認方法，都是仰賴於核磁共振（Nuclear Magnetic Resonance, NMR）與傅立葉轉換紅外光譜（Fourier Transform Infrared Spectroscopy, FTIR）的檢測，但這兩者的費用昂貴且耗時，並需要專業人員操作才可以完成。
本研究發現日落黃（Sunset yellow FCF）、胭脂紅（Ponceau 4R）、檸檬黃（Tartrazine）三種螯合性食用色素與幾丁聚醣反應後吸光值會有下降的表現，以吸光值變化量最大的日落黃作為實驗指試劑，日落黃吸光值變化量（△A500）與幾丁聚醣的去乙醯率有高度正相關（R2=0.9718）的表現，不受幾丁聚醣分子量影響，經過最佳化後以pH 4.0、20 mM 醋酸緩衝溶液，幾丁聚醣濃度10 mg/L為本研究的實驗條件，能有穩定實驗結果表現。 最後以不同去乙醯率與分子量（10-1000 kDa）的幾丁聚醣共25個樣品製成檢量線（R2=0.9417），並以未知去乙醯率的幾丁聚醣樣品進行實測，並與13C-NMR檢測結果進行比對，去除一個幾丁聚醣的顏色因素造成誤判，其餘樣品的檢測誤差均小於2%。整體檢測流程僅需1分鐘，是有潛力作為一種新的去乙醯率的快速檢測方法。 | zh_TW |
| dc.description.abstract | Chitosan is an important biomaterial used widely in tissue engineering and environmental application. Degree of deacetylation (DD%) is the most important quality parameter of chitosan. Several methods have been developed to determine DD% for chitosan. NMR (Nuclear Magnetic Resonance) and FTIR (Fourier Transform Infrared Spectroscopy) are typical method for detecting DD%. However, these methods are both expensive and time-consuming.
In this study, we developed a rapid and low-cost method to detect DD% by UV-VIS spectroscopy. We found that Sunset yellow FCF (SY), Ponceau 4R (P4R) and Tartrazine can chelate with chitosan, and have absorbance change. Because SY absorbance change is higher than P4R and Tartrazine, we chose SY as our indicator. The result showed that SY absorbance change had positive correlation (R2=0.9718) with DD%. In addition, it is not influenced by Chitosan molecular weight. After method optimization, pH 4.0 20 mM Acetic buffer and 10 mg/L chitosan are the best for DD% determination. Finally, We use 25 chitosan samples(MW=10-1000 kDa) to produce a calibration curve (R2=0.9417). Compared our mehod with 13C-NMR results to detect unknown DD% chitosan samples and their results had an error in less than 2 %, except for one sample which its yellow color had the effect on our detection. The process takes only 1 min and it has the potential for DD% rapid screening in the future. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:21:35Z (GMT). No. of bitstreams: 1 ntu-107-R05631017-1.pdf: 1574206 bytes, checksum: e33ab2b88570addf512df89261d88c34 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 中文摘要 i
Abstract ii 目錄 iii 圖目錄 vi 表目錄 viii 第一章 前言 1 第二章 文獻探討 2 2.1 色素的介紹與分類 2 2.1.1 天然色素 2 2.1.2 人工色素 3 2.1.2.1 Azo dye（雙氮偶合色素） 3 2.1.2.2 Sulfur dye（硫化色素） 4 2.1.2.3 Nitro and Nitroso Dye（硝基色素與亞硝基色素） 4 2.2 螯合性食用色素 5 2.2.1 常見螯合性食用色物與金屬反應後的光譜表現 6 2.2.1.1 Ponceau 4R（胭脂紅） 6 2.2.1.2 Sunset yellow FCF（日落黃） 7 2.2.1.3 Tartrazine（檸檬黃） 8 2.3 幾丁聚醣 9 2.3.1 去乙醯率 10 2.3.1.1 去乙醯方法 10 2.3.1.2 去乙醯率對幾丁聚醣的影響 12 2.3.2 環境pH 14 2.3.3 分子量 15 2.4 去乙醯率檢測方法 16 2.4.1 光譜法（Spectroscopy method） 16 2.4.2 傳統方法（Conventional method） 18 2.4.3 裂解法（Destructive method） 19 2.5 幾丁聚醣與螯合性色素之間的關係 20 第三章 材料與方法 22 3.1 化學藥品 22 3.2 試劑與緩衝溶液配置 23 3.2.1 試劑配製 23 3.2.2 緩衝溶液配置 24 3.3 實驗儀器設備 25 3.4 螯合性食用色素與幾丁聚醣間的吸光變化 26 3.5 探討日落黃與幾丁聚醣間關係 27 3.5.1 幾丁聚醣濃度對日落黃吸光影響 27 3.5.2 去乙醯率對日落黃吸光影響 28 3.5.3 分子量的影響 28 3.6 日落黃與幾丁聚醣實驗最佳化 29 3.6.1 幾丁聚醣實驗濃度 29 3.6.2 pH的影響 29 3.6.3 緩衝溶液濃度 30 3.7 樣品實測 31 第四章 結果與討論 32 4.1 螯合性食用色素與幾丁聚醣的吸光變化 32 4.2 探討日落黃與幾丁聚醣間的關係 35 4.2.1 幾丁聚醣濃度對日落黃吸光影響 35 4.2.2 去乙醯率對日落黃吸光影響及檢測波長選擇 36 4.2.3 幾丁聚醣分子量影響 39 4.3 日落黃與幾丁聚醣實驗條件最佳化 40 4.3.1 幾丁聚醣操作濃度 40 4.3.2 環境pH 41 4.3.3 緩衝溶液的濃度 43 4.4 檢量線及樣品實測 45 第五章 結論 48 參考文獻 49 | |
| dc.language.iso | zh-TW | |
| dc.subject | 雙氮偶合色素 | zh_TW |
| dc.subject | 快速檢測 | zh_TW |
| dc.subject | 日落黃 | zh_TW |
| dc.subject | 去乙醯率 | zh_TW |
| dc.subject | 幾丁聚醣 | zh_TW |
| dc.subject | degree of deacetylation | en |
| dc.subject | chitosan | en |
| dc.subject | sunset yellow FCF | en |
| dc.subject | azo dye | en |
| dc.subject | rapid screening. | en |
| dc.title | 螯合性食用色素於生物分子檢測之應用 | zh_TW |
| dc.title | Bio-molecular Analysis Using Food Dyes with Chelating
Capabilities | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 謝博全,賴盈璋,鄭宗記,林煥彰 | |
| dc.subject.keyword | 幾丁聚醣,去乙醯率,日落黃,雙氮偶合色素,快速檢測, | zh_TW |
| dc.subject.keyword | degree of deacetylation,chitosan,sunset yellow FCF,azo dye,rapid screening., | en |
| dc.relation.page | 55 | |
| dc.identifier.doi | 10.6342/NTU201803969 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-08-18 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
| 顯示於系所單位： | 生物機電工程學系 | |
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