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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 黃偉邦(Wei-Pang Huang) | |
| dc.contributor.author | Shue-Ying Lau | en |
| dc.contributor.author | 劉澍瑩 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:19:07Z | - |
| dc.date.available | 2019-08-21 | |
| dc.date.copyright | 2018-08-21 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-08-20 | |
| dc.identifier.citation | References
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/72013 | - |
| dc.description.abstract | 自噬作用為一個對維持細胞內物質恆定非常重要的自分解過程，其可協助細胞應對細胞內部養分的缺乏和細胞外部的環境壓力。自噬作用進行時，一部份的胞質由雙層膜構造的自噬體包裹，並與濾泡結合進而分解。很多蛋白質及其複合體參與其過程。前人研究發現內膜運輸機制亦參與調控自噬作用。Ypt/Rab GTP酶蛋白家族 (Ypt/Rab GTPase protein family) 為其中一個調控內膜運輸機制的重要蛋白質複合物。其中Ypt1和Ypt7均參與自噬作用的調控，Ypt1可調控自噬作用起始過程中的Atg1/Atg13複合物之生成，而Ypt7則為自噬體與濾泡結合的必需因子。Ypt/Rab GTP酶蛋白家族的活性由鳥苷酸轉換因子 (guanine-nucleotide exchange factors) 及GTP酶活化蛋白 (GTPase activation proteins) 調節。其中GTP酶活化蛋白Gyp1被認為是細胞中調控Ypt1及Ypt7的重要蛋白。本研究中，我們發現缺乏Gyp1的細胞會出現自噬作用缺陷，且當細胞缺乏Gyp1時，內膜運輸機制會失衡。 | zh_TW |
| dc.description.abstract | Autophagy is a self-degradation process that is important to sustain cell homeostasis responding to nutrient stresses and extracellular signals. During autophagy, a part of cytoplasm is engulfed by double-membrane autophagosomes, which fuse with the vacuole for degradation. Many protein complexes are involved. Several researches have proved that membrane trafficking pathway is involved in autophagy regulation. The Ypt/Rab GTPase protein family is one of the important protein complex regulating membrane trafficking. Previous studies have shown that Ypt1 and Ypt7 could both regulate autophagy process. Ypt1 regulates the formation of Atg1/Atg13 complex, a requirement of autophagy induction. On the other hand, Ypt7 is the essential factor for fusion between autophagosomes and the vacuole. Activity of Ypt/Rab GTPase proteins are modulated by guanine-nucleotide exchange factors (GEFs) and GTPase activation proteins (GAPs). Gyp1 is the GAP protein of Ypt1 and Ypt7 in yeast. In my study, gyp1D cells showed bulk autophagy and Cvt pathway defect. gyp1D cells also had conventional membrane trafficking defect phenotype. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:19:07Z (GMT). No. of bitstreams: 1 ntu-107-R05B21033-1.pdf: 4005205 bytes, checksum: eeba9e8f31f77e05c54c629cdf988f2e (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 中文摘要 iv Abstract v Contents vi 1 Introduction 1 1.1 Overview of autophagy 1 1.2 Overview of Ypt/Rab GTPase and GAP family proteins 4 2 Materials and Methods 6 2.1 Strains and media 6 2.2 Preparation of whole yeast cell extracts for immunoblotting analysis 6 2.3 CPY secretory analysis 7 2.4 Fluorescence microscopy 7 2.5 Pho8D60 assay 8 3 Results 10 3.1 GYP1 gene deletion cells show non-selective autophagy defect 10 3.2 Cells of a gyp1D strain showed Cvt pathway defect 12 3.3 gyp1D cells show conventional membrane trafficking defect phenotype 13 3.4 Ypt1 affects GFP-Atg8 distribution during autophagy progression 15 3.5 Ypt7 affects GFP-Atg8 distribution during autophagy progression 16 3.6 GFP-Gyp1 puncta observed in wild type cells after nitrogen starvation-induced 17 3.7 Atg1, Atg5 and Atg7 showed GFP signal mislocalization during nitrogen starvation in gyp1D cells 18 4 Disscusion 20 4.1 gyp1D cells display moderate autophagy defect and minor Cvt pathway defect 20 4.2 Over-expression of Ypt1 and Ypt7 differently affect gyp1D phenotype 20 4.3 gyp1D cells may affect the steps of autophagy induction 23 5. Conclusion 25 6 References 26 7. Schemes 34 8. Figures and Tables 38 | |
| dc.language.iso | en | |
| dc.title | Gyp1透過改變內膜運輸機制調控自噬作用之進程 | zh_TW |
| dc.title | Gyp1 regulates the autophagy pathway via modulating intracellular membrane trafficking | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 羅凱尹(Kai-Yin Lo),董桂書(Kuei-Shu Tung),朱家瑩(Chia-Ying Chu) | |
| dc.subject.keyword | 細胞自噬,內膜運輸機制,Ypt/Rab GTP?蛋白家族,GTP?活化蛋白,Ypt1,Ypt7,Gyp1, | zh_TW |
| dc.subject.keyword | Autophagy,membrane trafficking pathway,Ypt/Rab GTPase protein family,GTPase activation proteins (GAPs),Ypt1,Ypt7,Gyp1, | en |
| dc.relation.page | 56 | |
| dc.identifier.doi | 10.6342/NTU201804004 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-08-20 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| dc.date.embargo-terms | 2300-01-01 | |
| dc.date.embargo-lift | 2300-01-01 | - |
| Appears in Collections: | 生命科學系 | |
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| ntu-107-1.pdf Restricted Access | 3.91 MB | Adobe PDF |
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