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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 葉開溫(Kai-Wun Yeh) | |
| dc.contributor.author | Hsu-Tung Tsai | en |
| dc.contributor.author | 蔡旭東 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:15:47Z | - |
| dc.date.available | 2024-07-10 | |
| dc.date.copyright | 2019-07-10 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-08-13 | |
| dc.identifier.citation | 林于倫。2008。電照週期對文心蘭'Gower Ramsey'花序發育及品質之影響。國立中興大學園藝學系所學位論文。台中。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71939 | - |
| dc.description.abstract | 文心蘭(Oncidesa)為台灣外銷切花出口最大宗之花卉作物，但其開花與從營養 生長轉為生殖生長之機制尚未明瞭。前人研究發現，當文心蘭長時間處於中高環境 溫度(30°C)之下將會被誘導開花，因植物體內會大量累積活性氧化物質(reactive oxygen species; ROS)，並活化了維生素C-榖胱甘肽循環(AsA-GSH cycle)，誘導文 心蘭SQUAMOSA PROMOTER BINDING PROTEIN-LIKEs(OgSPLs)表現，進而誘導 下游開花基因Flowering locus T(FT)、LEAFY(LFY)與APETALA 1 (AP1)表現，最終使文心蘭開花。近期研究指出，海藻糖-6-磷酸鹽生成酶(trehalose-6-phosphate synthase;TPS)會生合成海藻糖-6-磷酸鹽(T6P)，而T6P會作為一種信號分子，去協助植物抵 抗逆境、對養分脅迫進行反應與影響開花時間，並且T6P的含量與蔗糖含量呈現正 相關性。為了探究OgTPS1與前人發現之文心蘭中高環境溫度誘導開花路徑之關係， 並且探討OgTPS1與蔗糖之關係，我們將文心蘭處理了中高環境溫度、buthionine sulfoximine(BSO)、glutathione disulfide(GSSG)、glutathione(GSH)以及蔗糖(sucrose)， 隨後利用qPCR檢測相關基因之表現情況。從結果顯示，OgTPS1會受中高環境溫度、 BSO、GSSG、GSH以及蔗糖之誘導表現。同時檢測了OgSPLs與FT、LFY以及AP1 後，發現OgSPLs與FT、LFY以及AP1會受到中高環境溫度、BSO、GSSG與蔗糖處 理提高表現情況，反之受到GSH所抑制表現。關於此實驗結果，說明OgTPS1會協 助植物抵抗氧化逆境，並且其參與了前人發現之文心蘭中高環境溫度誘導開花途 徑，同時也可作為糖類誘導開花途徑的關鍵因子。 | zh_TW |
| dc.description.abstract | The mechanism of flowering and phase transition from vegetative to reproductive stage is a mysterious event in Oncidesa. In previous study, we found that high ambient temperature-induced flowering pathway in Oncidesa. This floral induction in response to thermal stress is associated with a significantly increased reactive oxygen species (ROS) level and active Ascorbate acid(ASA)-Glutathione(GSH) pathway. The redox signal will induce Oncidesa SQUAMOSAPROMOTER BINDING PROTEIN-LIKEs(OgSPLs), and then it will elevate the expression level of Flowering locus T(FT), LEAFY(LFY) and APETALA 1(AP1). Recent studies indicate that trehalose-6-phosphate(T6P), which is synthesized by trehalose-6-phosphate synthase(TPS), will be a signal molecule to influence stress tolerance and regulate flowering time. Researches point out that increasing sucrose levels will positively affect the abundance of T6P in plants. As mentioned above, we want to know whether OgTPS1 will be involved in high ambient temperature-induced flowering pathway in Oncidesa or not. We also want to explore the relationship between OgTPS1 and sucrose. Afterwards, we use high temperature, glutathione disulfide(GSSG), glutathione(GSH), buthionine sulfoximine(BSO) and sucrose to treat Oncidesa, and then we use qPCR to detect the expression level of several genes. Studies reveal that OgTPS1 is induced by high-ambient temperature, GSSG, GSH, BSO and sucrose. On the other hand, OgSPLs, FT, LFY and AP1 were induced by high- ambient temperature, GSSG, BSO and sucrose, and repressed by GSH. Taken together, OgTPS1 may confer plants with stress resistance, and is involved in high ambient temperature-induced flowering pathway. Moreover, OgTPS1 acts as a key factor in sugar induced flowering pathway. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:15:47Z (GMT). No. of bitstreams: 1 ntu-107-R05b42013-1.pdf: 9643536 bytes, checksum: 96312b8b699a39bee9a54468609ea34d (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 致謝......................................................................................................... ii
中文摘要...................................................................................................iii Abstract ..................................................................................................iv 圖表目錄...................................................................................................viii 附錄圖表目錄............................................................................................ix 第一章 前言...............................................................................................1 第一節 文心蘭概述.....................................................................................1 第二節 植物開花生理..................................................................................4 第三節 TPS1(trehalose-6-phosphatesynthase1)對植物生理之影響.............10 第四節 研究目的.........................................................................................14 第二章 材料與方法......................................................................................16 (A) 實驗材料 ..............................................................................................16 (B) 實驗方法 ..............................................................................................16 第一節 基因表現量測定...............................................................................16 第二節 BSO、GSSG以及GSH處理文心蘭.....................................................20 第三節 中高環境溫度處理文心蘭..................................................................20 第四節 蔗糖處理文心蘭................................................................................20 第五節 基因釣取..........................................................................................21 第三章 結果.................................................................................................23 第一節 OgTPS1基因之選殖、序列分析與親緣性分析.....................................23 第二節 OgTPS1於文心蘭中之表現情況.........................................................24 第三節 OgTPS1基因表現與氧化還原逆境(redoxhomeostasis)的關係.............25 第四節 OgTPS1 的基因表現與中高環境溫度及 BSO 之關係............................27 第五節 施用 50mM 蔗糖溶液對文心蘭開花與 OgTPS1 表現之影響 ................27 第四章 討論..................................................................................................29 第一節 OgTPS1於文心蘭中之表現情況..........................................................29 第二節 OgTPS1與中高環境溫度誘導開花路徑之關係......................................32 第三節 OgTPS1與蔗糖之關係........................................................................34 第四節 未來展望...........................................................................................36 參考文獻......................................................................................................37 | |
| dc.language.iso | zh-TW | |
| dc.subject | OgTPS1基因 | zh_TW |
| dc.subject | 中高環境溫度 | zh_TW |
| dc.subject | 蔗糖 | zh_TW |
| dc.subject | 開花 | zh_TW |
| dc.subject | 文心蘭 | zh_TW |
| dc.subject | flowering | en |
| dc.subject | high ambient temperature | en |
| dc.subject | sucrose | en |
| dc.subject | OgTPS1 | en |
| dc.subject | Oncidesa | en |
| dc.title | 探討OgTPS1基因對於調控文心蘭生長與 開花所扮演的功能 | zh_TW |
| dc.title | Functional analysis of OgTPS1(trehalose-6-phosphate synthesis 1) regulating growth and flowering
in Oncidesa Gower Ramsey ‘Honey Angel’ | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳賢明,邱崇益,詹明才,謝旭亮 | |
| dc.subject.keyword | OgTPS1基因,中高環境溫度,蔗糖,開花,文心蘭, | zh_TW |
| dc.subject.keyword | OgTPS1,high ambient temperature,sucrose,flowering,Oncidesa, | en |
| dc.relation.page | 70 | |
| dc.identifier.doi | 10.6342/NTU201803122 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-08-14 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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