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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 盧彥文(Yen-Wen Lu) | |
| dc.contributor.author | Jia-Hong Liu | en |
| dc.contributor.author | 劉家宏 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:13:58Z | - |
| dc.date.available | 2019-10-02 | |
| dc.date.copyright | 2018-10-02 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-09-25 | |
| dc.identifier.citation | Asbury, C. L. (2005). Kinesin: world's tiniest biped. Curr Opin Cell Biol, 17(1), 89-97. doi:10.1016/j.ceb.2004.12.002
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(2015). 温度応答性高分子を用いたキネシン・微小管系の往復運動デバイスの作製. 精密工学会学術講演会講演論文集, 813-814. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71896 | - |
| dc.description.abstract | 微管（MTs, or microtubules）在溫度梯度場下往單一方向進行聚合反應，這種環境與微管在軸突中的形成相似。因此，為了模擬這樣的環境，我們發展了一種具有加熱器、可以產生線性溫度梯度的微流體平台，這平台可以促使微管在晶片上進行聚合反應，並且控制其聚合方向。在實驗中，我們發現了微管在溫度梯度場中可以成功的聚合並整齊排列。同時，為了量化MT的排列情形，我們利用了以結構張量(structure tensor)的圖像處理技術。結果說明，在溫度梯度場下，微管排列集中在31.2度內的區間。相對應的，在均勻溫度場下，微管則廣泛分佈，較無方向性。 此外，我們研究在兩種不同的微管蛋白濃度下，微管的聚合情形與排列情況發現了，在高濃度環境下的微管聚合比在低濃度環境下的微管聚合，由於有較為足夠的微管蛋白濃度，微管聚合有著範圍較長的排列的距離。 | zh_TW |
| dc.description.abstract | Microtubules (MTs) were unidirectionally polymerized under a temperature gradient field - an environment which was similar to their formation in axon. We proposed a microfluidic platform with a triangular heater to generate a linear temperature gradient for on-chip MT polymerization. It was found that the MTs were polymerized and neatly aligned in the temperature gradient field. To quantify the alignment of MTs, an image processing technique based on structure-tensor were utilized. The results showed the MTs under the temperature gradient field had a better alignment within 31.2 degrees, whereas the MT under the uniform temperature field widely dispersed. Further, the MT polymerization and alignments at two different tubulin concentrations were examined. The MT polymerized at the higher concentration was aligned for a longer distance than the one at the lower concentration due to the sufficient tubulin concentration. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:13:58Z (GMT). No. of bitstreams: 1 ntu-107-R04631014-1.pdf: 4425732 bytes, checksum: 906f1f2eaaf7e0e52962a87d3ae76381 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 致謝 i
中文摘要 iii Abstract iv Table of Contents v List of Figure ix List of Table xvi Chapter 1 Introduction 1 1.1 Microtubule 2 1.2 Motor Proteins-Kinesin 3 1.3 MEMs Technology 4 1.4 Overall Structure of Thesis 5 Chapter 2 Literature Review 6 2.1 Microtubule Assembly and Organization 6 2.2 Motor Protein Kinesin 9 2.3 Kinesin Coating on Substrate 10 2.4 Motor Protein Kinesin Motility 12 2.5 Method for Controlling Direction 12 2.6 Aligned Protein Self-Assemblies 14 2.7 Tubulin Polymerization in Temperature Gradient 15 2.8 Microfluidic Concentration Gradients 16 Chapter 3 Materials and Methods 18 3.1 Principle of Alignment Polymerization 18 3.2 Design and Fabrication of Heating Chip 21 3.2.1 Design of Temperature Gradient heater 21 3.2.2 Fabrication of the Triangular Heater by ITO Glass 23 3.3 Heating Chip and Temperature Control Method 26 3.4 Tubulin Reagent Preparation 28 3.4.1 Tubulin Protein 28 3.4.2 Fluorescence-Labeled Tubulin 28 3.4.3 Tubulin Reagent Allocation 28 3.5 Experiment Setup 29 3.6 Microscopy and Observation Method 31 3.7 MTs Alignment Angle Analysis 32 Chapter 4 Result 34 4.1 The Performance of Heating Chip 34 4.1.1 The Performance of Triangular Heater 34 4.1.2 Thermal Gradient Calibration and Control 35 4.2 The Visualization of Microtubules 38 4.2.1 Centrifugal before Polymerization 39 4.2.2 Fluorescence-Labeled Tubulin: Unlabeled-Tubulin Ratio 41 4.2.3 GMPCPP Concentration 43 4.3 Polymerization Results Comparison in Uniform Temperature and Temperature Gradient 45 4.4 The Alignment Situation of MTs in the Temperature Gradient 51 4.4.1 MTs Polymerization in Gradient Temperature 39°C-34°C at Low Concentration 51 4.4.2 The Reason of Low MTs Density in Low Temperature Region 54 4.4.3 MTs Polymerization in Gradient Temperature 39°C-34°C at High Concentration 56 Chapter 5 Discussion 59 5.1 The Degree of MTs Alignment in a Temperature Gradient 59 5.2 The Density of MT Degraded in Temperature Gradient Field 60 5.3 Application of this Polymerization Method 61 5.4 Limitation of Analytical Technology 62 Chapter 6 Conclusion and Prospect 64 6.1 Conclusion 64 6.2 Prospect 65 Appendix I 67 Appendix II 68 Appendix III 70 Appendix IV 71 Reference 72 | |
| dc.language.iso | en | |
| dc.subject | 溫度梯度 | zh_TW |
| dc.subject | 微管 | zh_TW |
| dc.subject | 對準 | zh_TW |
| dc.subject | 纖維排列 | zh_TW |
| dc.subject | microtubule | en |
| dc.subject | temperature gradient | en |
| dc.subject | alignment | en |
| dc.subject | triangular heater | en |
| dc.title | 利用溫度梯度晶片系統構築微管聚合 | zh_TW |
| dc.title | Microtubule Polymerization Controlled by Temperature Gradient on a Microchip. | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 侯詠德(Yung-Te Hou),夏國強(Kuo-Chiang Hsia) | |
| dc.subject.keyword | 微管,溫度梯度,對準,纖維排列, | zh_TW |
| dc.subject.keyword | microtubule,temperature gradient,alignment,triangular heater, | en |
| dc.relation.page | 74 | |
| dc.identifier.doi | 10.6342/NTU201804141 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-09-26 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
| 顯示於系所單位： | 生物機電工程學系 | |
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