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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71804完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳振中(Chun Chung Chan) | |
| dc.contributor.author | Cheng-I Ho | en |
| dc.contributor.author | 何政逸 | zh_TW |
| dc.date.accessioned | 2021-06-17T06:10:24Z | - |
| dc.date.available | 2020-12-17 | |
| dc.date.copyright | 2018-12-17 | |
| dc.date.issued | 2018 | |
| dc.date.submitted | 2018-11-20 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/71804 | - |
| dc.description.abstract | 近年來蛋白質動態學越來越受人們重視，因為許多研究指出蛋白質的運動與其生物功能性相關，而核磁共振儀為眾多研究蛋白質動態學的方法中最常被使用的儀器之一。在本論文中我們認為雙量子同調性訊號與分子動態有直接關係，因此我們嘗試設計不同的脈衝序列以偵測該訊號於磁場中的演化。我們嘗試了三種方法，其中以脈衝序列 SPC5 激發出雙量子同調性訊號、並再以脈衝序列 R-TOBSY 對訊號進行鎖場的方法有最佳結果。我們運用 R-TOBSY 的對稱性抑制除了 J-耦合之外的作用力，讓雙量子訊號隨鎖場時間的衰減只與系統性自旋-自旋弛緩現象以及分子動態相關。實驗中，樣品 [U-13C]-L-丙胺酸之實驗結果與軟體模擬出的結果吻合，且鎖場時間可長達 6 毫秒，顯示此方法的成功。最後我們將此方法運用於蛋白質 GB1 以及 Bcl-xL，並使用三角與指數之合成函數對訊號擬合，得到各標示峰的雙量子同調性訊號衰減速率。雖然因為光譜解析度不足而無法再進一步與胺基酸位置對應，一旦能克服此問題，此方法將有機會得到蛋白質各部位的動態資訊。 | zh_TW |
| dc.description.abstract | Motional dynamics in polypeptides and proteins are believed to correlate with their biological functions. Such dynamics may be indirectly characterized by probing the double quantum coherence (DQC) signals in NMR. We attempt to generate DQC with pulse sequence SPC5 in solid-state NMR. Three methods have been applied to investigate the evolution of DQC. Among them, the method using R-TOBSY to do spin-locking gave the most favorable results. Effects from the dipolar interactions and chemical shifts are suppressed by the symmetry of R-TOBSY, so that the decay of DQC signals only depend on the transverse spin-spin relaxation properties of the systems. Experimental data from [U-13C]-L-alanine agree with the simulated curves at a spin-locking time up to 6 milliseconds. We then apply this method on protein GB1 and Bcl-xL. The resulted signals are fitted with a mathmatic function including oscillation part and decay part. Though the low resolution of spectra limits the implication of fitting, as long as we conquer this problem the R-TOBSY spin-locking method may be applicable to study the motional dynamics of biomacromolecules. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T06:10:24Z (GMT). No. of bitstreams: 1 ntu-107-R05223107-1.pdf: 5274649 bytes, checksum: 5b47c159d1bc821e33827475a44fed51 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 第一章：蛋白質動力學 1
1.1. 蛋白質之動態與功能性 1 1.2. 核磁共振儀於蛋白質動態學之應用 3 第二章：固態核磁共振儀 6 2.1. 磁矩與弛緩現象 6 2.1.1. 磁矩 6 2.1.2. 弛緩現象 7 2.2. 固態核磁共振儀中的作用力 9 2.2.1. 塞曼效應 9 2.2.2. 化學位移 10 2.2.3. 偶極-偶極作用力 12 2.2.4. J-耦合 13 2.3. 魔術角旋轉 14 2.4. 作用力之選擇 15 2.4.1. 平均哈密頓理論 15 2.4.2. 作用力框架 15 2.4.3. 脈衝序列的對稱性 17 2.5. 固態核磁共振儀中的技巧 20 2.5.1. 交叉極化 20 2.5.2. 同核偶極耦合 21 2.5.3. γ-encoded 脈衝序列 22 2.5.4. 脈衝序列 SPC5 之介紹 23 2.5.5. 脈衝序列 R-TOBSY 之介紹 25 第三章：實驗方法 27 3.1. 蛋白質的表達與純化 27 3.1.1. 蛋白表達原理 27 3.1.2. 蛋白質 GB1 27 3.1.3. 蛋白質 Bcl-xL 30 3.2. 稀釋同位素標定之固態樣品 34 3.3. 自旋-晶格之弛緩時間 34 3.3.1. 測量自旋-晶格之弛緩時間 34 3.3.2. 減少自旋-晶格之弛緩時間 35 3.4. 固態 NMR 條件 36 3.5. 利用 SPC5 激發雙量子同調性訊號 36 3.5.1. SPC5 光譜之相位調整 36 3.5.2. 利用 SPC5 激發雙量子同調性訊號 37 3.5.3. 設計固定時間之 SPC5 40 3.6. 利用 R-TOBSY 進行雙量子訊號之鎖場 41 3.7. 模擬軟體 SIMPSON 42 3.7.1. 原理 43 3.7.2. 軟體操作 44 3.7.3. 模擬參數 45 3.8. 以 NMRPIPE 處理光譜 47 第四章：結果與討論 48 4.1. 蛋白質表達之純化與鑑定 48 4.1.1. 蛋白質 GB1 48 4.1.2. 蛋白質 Bcl-xL 53 4.2. 利用 SPC5 激發之雙量子訊號演化 57 4.2.1. SIMPSON 模擬 58 4.2.2. 實驗與模擬之對應 59 4.3. 固定時間 SPC5 激發之雙量子訊號演化 61 4.3.1. SIMPSON 模擬值 61 4.3.2. 實驗與模擬之對應 63 4.4. 利用R-TOBSY對雙量子訊號鎖場 66 4.4.1. SIMPSON 模擬值 66 4.4.2. 實驗與模擬之對應 68 4.4.3. 樣品中加入銅以及溶液的影響 70 4.5. 將 R-TOBSY 鎖場方法運用至蛋白質樣品 74 4.5.1. GB1 固態樣品 74 4.5.2. 含銅之 GB1 樣品 80 4.5.3. Bcl-xL 沉澱樣品 82 第五章：結論 88 參考文獻 90 附錄 A 使用 SIMPSON 模擬軟體之脈衝序列 99 A.1 SPC5 激發雙量子同調性訊號 99 A.2 設計固定時間之 SPC5 100 A.3 利用 R-TOBSY 進行雙量子訊號之鎖場 102 附錄 B 以 NMRPIPE 與 SPARKY 處理光譜之比較 104 附錄 C 蛋白質 GB1 的峰值定標 106 | |
| dc.language.iso | zh-TW | |
| dc.subject | 核磁共振 | zh_TW |
| dc.subject | 雙量子 | zh_TW |
| dc.subject | 鎖場 | zh_TW |
| dc.subject | 固態核磁共振 | zh_TW |
| dc.subject | solid state NMR | en |
| dc.subject | NMR | en |
| dc.subject | nuclear magnatic resonance | en |
| dc.subject | double quantum | en |
| dc.subject | spin locking | en |
| dc.title | 雙量子同調性於固態核磁共振儀中的演化與鎖場 | zh_TW |
| dc.title | Evolution and Spin-locking of Double Quantum Coherence in Solid-state NMR | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 107-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 徐尚德(Shang-Te Hsu),牟昀(Mou Yun) | |
| dc.subject.keyword | 固態核磁共振,核磁共振,雙量子,鎖場, | zh_TW |
| dc.subject.keyword | solid state NMR,NMR,nuclear magnatic resonance,double quantum,spin locking, | en |
| dc.relation.page | 107 | |
| dc.identifier.doi | 10.6342/NTU201804274 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-11-21 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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