可編程高分子散射液晶陣列應用於共軛焦顯微鏡

Abstract

共軛焦顯微鏡是能提升顯微鏡影像解析度與對比度的重要關鍵，特別是應用在螢光顯微鏡上。可程式續陣列顯微鏡是一種利用可程式續的空間光調製器的共軛焦顯微鏡。先前利用數位微鏡裝置或單晶矽反射式液晶在共軛焦顯微鏡上已被廣泛的演示。單晶矽反射式液晶雖然沒有機械部件，但需要極化光雷射會讓光的使用率下降。在本篇論文中，我們提出利用高分子散射液晶當作空間光調製器的可程式續陣列顯微鏡。相較於數位微鏡裝置或單晶矽反射式液晶的共軛焦顯微鏡，高分子散射液晶空間光調製器容易製造、花費較少，且系統容易架設。此外，在本篇系統中，高分子散射液晶空間光調製器垂直於光軸，是在其他系統中無法做到或需要更多的元件。因此在本篇系統終能讓各元件更緊密排列，並相較於其他以液晶為主的系統能增加動態範圍。

The confocal microscopy is the based concept to improve the resolution of the microscopy image, and widely utilized in fluorescence microscopy. Programmable Array Microscopy (PAM) is one of confocal microscopy utilizing programmable spatial light modulator (SLM). Previously, utilizing Digital Micromirror Device (DMD) or Liquid Crystal On Silicon (LCOS) as SLM for confocal microscopy has been widely demonstrated. LCOS has no mechanical part in the component, but polarizing laser is needed causing poor light utilizing rate. In this thesis, we present a PAM which uses the polymer-dispersed liquid crystal (PDLC) chip as the SLM. PDLC SLM is easier to fabricate, cost more affordable, and easier to setup the system than other LCOS or DMD based PAMs. Furthermore, in our system, the incident light is perpendicular to the PDLC chip, while others can’t or need more components. Therefore, our system will make the whole system more compact. Also, it can increase the dynamic range compare to other liquid crystal based PAMs.