請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70604
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝旭亮 | |
dc.contributor.author | Guan-Han Li | en |
dc.contributor.author | 李觀漢 | zh_TW |
dc.date.accessioned | 2021-06-17T04:32:19Z | - |
dc.date.available | 2020-08-14 | |
dc.date.copyright | 2018-08-14 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-10 | |
dc.identifier.citation | Alba, R., Payton, P., Fei, Z., McQuinn, R., Debbie, P., Martin, G.B., Tanksley, S.D., andGiovannoni, J.J. (2005). Transcriptome and selected metabolite analyses reveal multiple points of ethylene control during tomato fruit development. Plant Cell 17: 2954–2965.
Bemer, M., Karlova, R., Ballester, A.R., Tikunov, Y.M., Bovy, A.G., Wolters-Arts, M., Rossetto, P. d. B., Angenent, G.C., anddeMaagd, R.A. (2012). The Tomato FRUITFULL Homologs TDR4/FUL1 and MBP7/FUL2 regulate ethylene-independent aspects of fruit ripening. Plant Cell 24: 4437–4451. Bianchetti, R.E., Silvestre Lira, B., Santos Monteiro, S., Demarco, D., Purgatto, E., Rothan, C., Rossi, M., andFreschi, L. (2018). Fruit-localized phytochromes regulate plastid biogenesis, starch synthesis, and carotenoid metabolism in tomato. J. Exp. Bot. Busi, M.V., Bustamante, C., D’Angelo, C., Hidalgo-Cuevas, M., Boggio, S.B., Valle, E.M., andZabaleta, E. (2003). MADS-box genes expressed during tomato seed and fruit development. Plant Mol. Biol. 52: 801–15. Cheng, M.-Y. (2015). Functional Studies of Tomato TDR4 in the Integration of Blue Light and Jasmonate Signaling Pathway. 國立臺灣大學生命科學院植物科學研究所 碩士論文: 1–66. Ditta, G., Pinyopich, A., Robles, P., Pelaz, S., andYanofsky, M.F. (2004). The SEP4 gene of Arabidopsis thaliana functions in floral organ and meristem identity. Curr. Biol. 14: 1935–1940. Dong, T., Hu, Z., Deng, L., Wang, Y., Zhu, M., Zhang, J., andChen, G. (2013). A Tomato MADS-Box Transcription Factor, SlMADS1, acts as a negative regulator of fruit ripening. Plant Physiol. 163: 1026–1036. Dumas, Y., Dadomo, M., DiLucca, G., andGrolier, P. (2003). Effects of environmental factors and agricultural techniques on antioxidant content of tomatoes. J. Sci. Food Agric. 83: 369–382. Fernandez-Pozo, N., Menda, N., Edwards, J.D., Saha, S., Tecle, I.Y., Strickler, S.R., Bombarely, A., Fisher-York, T., Pujar, A., Foerster, H., Yan, A., andMueller, L.A. (2015). The Sol Genomics Network (SGN)-from genotype to phenotype to breeding. Nucleic Acids Res. 43: D1036–D1041. Fujisawa, M., Nakano, T., andIto, Y. (2011). Identification of potential target genes for the tomato fruit-ripening regulator RIN by chromatin immunoprecipitation. BMC Plant Biol. 11: 26. Fujisawa, M., Shima, Y., Nakagawa, H., Kitagawa, M., Kimbara, J., Nakano, T., Kasumi, T., andIto, Y. (2014). Transcriptional regulation of fruit ripening by tomato FRUITFULL homologs and associated MADS Box proteins. Plant Cell 26: 89–101. Giliberto, L., Perrotta, G., Pallara, P., Weller, J.L., Fraser, P.D., Bramley, P.M., Fiore, A., Tavazza, M., andGiuliano, G. (2005). Manipulation of the blue light photoreceptor Cryptochrome 2 in tomato affects vegetative development, flowering time, and fruit antioxidant content. Plant Physiol. 137: 199–208. Gramzow, L. andTheissen, G. (2010). A hitchhiker’s guide to the MADS world of plants. Genome Biol. 11:214 Gupta, S.K., Sharma, S., Santisree, P., Kilambi, H.V., Appenroth, K., Sreelakshmi, Y., andSharma, R. (2014). Complex and shifting interactions of phytochromes regulate fruit development in tomato. Plant, Cell Environ. 37: 1688–1702. Hirschberg, J. (2001). Carotenoid biosynthesis in flowering plants. Curr. Opin. Plant Biol. 4: 210–218. Ito, Y. (2016). Regulation of tomato fruit ripening by MADS-box transcription factors. Japan Agric. Res. Q. 50: 33–38. Jiao, Y., Lau, O.S., andDeng, X.W. (2007). Light-regulated transcriptional networks in higher plants. Nat. Rev. Genet. 8: 217–230. Kircher, S., Nobis, T., Nitschke, R., Kunkel, T., Bauer, D., Schäfer, E., Viczián, A., Nagy, F., Ádám, É., andPanigrahi, K.C.S. (2004). Constitutive photomorphogenesis 1 and multiple photoreceptors control degradation of phytochrome interacting factor 3, a transcription factor required for light signaling in Arabidopsis. 16: 1433–1445. Klee, H.J. andGiovannoni, J.J. (2011). Genetics and control of tomato fruit ripening and quality attributes. Annu. Rev. Genet. 45: 41–59. Lee, T.-C. (2006). Identification of blue light-induced genes involved in the lycopene accumulation of Lycopersicon esculentum fruit. 國立台灣大學生命科學院植物科學研究所 碩士論文. Leivar, P. andMonte, E. (2014). PIFs: Systems Integrators in Plant Development. Plant Cell 26: 56–78. Leseberg, C.H., Eissler, C.L., Wang, X., Johns, M.A., Duvall, M.R., andMao, L. (2008). Interaction study of MADS-domain proteins in tomato. J. Exp. Bot. 59: 2253–2265. Li, J., Li, G., Wang, H., andWang Deng, X. (2011). Phytochrome Signaling Mechanisms. Arab. B. 9: e0148. Lieberman, M., Segev, O., Gilboa, N., Lalazar, A., andLevin, I. (2004). The tomato homolog of the gene encoding UV-damaged DNA binding protein 1 (DDB1) underlined as the gene that causes the high pigment-1 mutant phenotype. Theor. Appl. Genet. 108: 1574–1581. Liu, L., Shao, Z., Zhang, M., andWang, Q. (2015). Regulation of carotenoid metabolism in tomato. Mol. Plant 8: 28–39. Liu, Y., Roof, S., Ye, Z., Barry, C., vanTuinen, A., Vrebalov, J., Bowler, C., andGiovannoni, J. (2004). Manipulation of light signal transduction as a means of modifying fruit nutritional quality in tomato. Proc. Natl. Acad. Sci. USA 101: 9897–9902. Llorente, B., D’Andrea, L., Ruiz-Sola, M.A., Botterweg, E., Pulido, P., Andilla, J., Loza-Alvarez, P., andRodriguez-Concepcion, M. (2016). Tomato fruit carotenoid biosynthesis is adjusted to actual ripening progression by a light-dependent mechanism. Plant J. 85: 107–119. Lozano, R., Giménez, E., Cara, B., Capel, J., andAngosto, T. (2009). Genetic analysis of reproductive development in tomato. Int. J. Dev. Biol. 53: 1635–1648. Lu, S. andLi, L. (2008). Carotenoid metabolism: Biosynthesis, regulation, and beyond. J. Integr. Plant Biol. 50: 778–785. Pedmale, U.V, Huang, S.C., Zander, M., Nery, J.R., Ecker, J.R., Huang, S.C., Zander, M., Cole, B.J., Hetzel, J., andLjung, K. (2016). Cryptochromes interact directly with PIFs to control plant growth in limiting blue light article cryptochromes interact directly with PIFs to control plant growth in limiting blue light.: 1–13. Ronen, G., Cohen, M., Zamir, D., andHirschberg, J. (1999). Regulation of carotenoid biosynthesis during tomato fruit development: expression of the gene for lycopene epsilon cyclase is down- regulated during ripening and is elevated in the mutant Delta. Plant J. 17: 341–351. Rosado, D., Gramegna, G., Cruz, A., Lira, B.S., Freschi, L., DeSetta, N., andRossi, M. (2016a). Phytochrome Interacting Factors (PIFs) in Solanum lycopersicum: Diversity, evolutionary history and expression profiling during different developmental processes. PLoS One 11: 1–21. Rosado, D., Gramegna, G., Cruz, A., Lira, B.S., Freschi, L., DeSetta, N., andRossi, M. (2016b). Phytochrome Interacting Factors (PIFs) in Solanum lycopersicum: diversity, evolutionary history and expression profiling during different developmental processes.: 1–21. Schofield, A. andPaliyath, G. (2005). Modulation of carotenoid biosynthesis during tomato fruit ripening through phytochrome regulation of phytoene synthase activity. Plant Physiol. Biochem. 43: 1052–1060. Schroeder, D.F., Gahrtz, M., Maxwell, B.B., Cook, R.K., Kan, J.M., Ecker, J.R., andChory, J. (2002). De-Etiolated 1 and damaged DNA binding protein 1 interact to regulate. Current 12: 1462–1472. Shima, Y., Fujisawa, M., Kitagawa, M., Nakano, T., Kimbara, J., Nakamura, N., Shiina, T., Sugiyama, J., Nakamura, T., Kasumi, T., andIto, Y. (2014). Tomato FRUITFULL homologs regulate fruit ripening via ethylene biosynthesis. Biosci. Biotechnol. Biochem. 78: 231–237. Song, Y., Yang, C., Gao, S., Zhang, W., Li, L., andKuai, B. (2014). Age-Triggered and Dark-Induced leaf senescence require the bHLH transcription factors PIF3, 4, and 5. Mol. Plant 7: 1776–1787. Toledo-Ortiz, G., Huq, E., andRodriguez-Concepcion, M. (2010). Direct regulation of phytoene synthase gene expression and carotenoid biosynthesis by phytochrome-interacting factors. Proc. Natl. Acad. Sci. USA 107: 11626–11631. Tseng, Y.-Y. (2010). Functional study of a light-induced factor TDR4 affecting lycopene levels in tomato fruit. 國立台灣大學生命科學院植物科學研究所 碩士論文: 1–75. Wang, S., Lu, G., Hou, Z., Luo, Z., Wang, T., Li, H., Zhang, J., andYe, Z. (2014). Members of the tomato FRUITFULL MADS-box family regulate style abscission and fruit ripening. J. Exp. Bot. 65: 3005–3014. Wu, J.-L. (2012). Functional study of the molecular mechanism underlying transcription factor TDR4-regulated lycopene accumulation in tomato fruit. 國立台灣大學植物科學研究所 碩士論文: 1–62. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70604 | - |
dc.description.abstract | 番茄紅熟過程受環境與植物賀爾蒙所影響,紅熟過程中由綠轉紅是透過胡蘿蔔素生合成路徑所產生的茄紅素所致,而開啟此路徑之重要酵素為PSY (Phytoene synthase),它受環境與賀爾蒙所調控之轉錄因子所影響,透由PSY的啟動而產生茄紅素累積;在先前研究中,TDR4為調控PSY1的轉錄因子之一且受到乙烯調節;但我們研究中也發現藍光可誘導TDR4的表現,進而影響茄紅素的大量累積,另一轉錄因子PIF1a也可與PSY1的啟動子結合而抑制其表現,來降低茄紅素產生,且PIF1a為參與在光訊息傳遞之中的轉錄因子;為了瞭解這二個基因如何參與在茄紅素生合成路徑,我們透過基因表現與蛋白質交互作用來瞭解。基因表現結果中顯示TDR4可以促進PIF1a的表現,並於PIF1a的啟動子區域發現可供TDR4結合的CArG序列。由Electrophoretic mobility shift assay (EMSA)結果顯示TDR4對該序列有結合專一性,利用重組蛋白質進行pull-down assay與Bimolecular Fluorescence Complementation (BiFC)分析中發現TDR4可與PIF1a結合且位於細胞核中。因此,兩者的交互作用可能會影響茄紅素累積,詳細機制仍有待進一步研究。 | zh_TW |
dc.description.abstract | The ripening process in tomato fruit is regulated by environmental factors and plant hormones. Tomato fruit turns from green into red color that is due to lycopene accumulation. The lycopene is synthesized from the carotenoid pathway and this pathway is mainly controlled by an enzyme called phytoene synthase (PSY). Environmental factors and plant hormones trigger transcription factors that can induce or reduce PSY gene expression to regulate tomato ripening. In previous studies, the transcription factor TDR4 participated in the regulation of carotenoid biosynthetic pathway. TDR4 triggered by ethylene is well known. But we found that TDR4 could be induced by blue light. However, the mechanisms that integrate light and ethylene signaling are unknown. Another transcription factor regulated by light is PIF1a. PIF1a can reduce PSY1 gene expression and lycopene content. To further understand how these two genes regulate PSY1 expression, resulting in lycopene accumulation and fruit ripening, we perform gene expression and protein-protein interaction studies. The results derived from qPCR data indicated that TDR4 might up-regulate PIF1a gene expression. We found that the CArG-motif bound by TDR4 is also present in the promoter region of PIF1a. The Electrophoretic mobility shift assay (EMSA) confirmed that TDR4 could specifically bind to the PIF1a promoter. Pull-down assays and Bimolecular Fluorescence Complementation (BiFC) studies indicated that TDR4 interacted with PIF1a in the nucleus. Thus, it may be interesting to understand the molecular mechanisms underlying TDR4 and PIF1a interaction in regulating lycopene accumulation in tomato fruit in the near future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:32:19Z (GMT). No. of bitstreams: 1 ntu-107-R04b42026-1.pdf: 2081100 bytes, checksum: 48bf1eee38180243e57f5cf354f8ff16 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 誌謝 I
摘要 II Abstract III 目錄 V 前言 1 一、番茄簡介 1 二、類胡蘿蔔素(carotenoid)之生合成路徑及其調控機制 1 三、光調控植物生長發育 3 四、TDR4轉錄因子 4 五、PIFs轉錄因子 4 六、研究動機 5 實驗材料與方法 6 一、植物材料及生長條件 6 二、RNA萃取與基因表現測定 6 三、質體構築 7 四、Electrophoretic mobility shift assay (EMSA) 7 五、蛋白質交互作用測試(Pull-down assay) 8 六、次細胞層次座落位置實驗 (Subcellular localization) 8 七、雙分子螢光互補實驗(Bimolecular fluorescence complementation,BiFC) 8 結果 9 一、Micro-Tom番茄的果實有TDR4與PIF1a的基因表現 9 二、在Micro-Tom番茄中不同時期的果實基因表現中TDR4與PIF1a均隨果實紅熟而下降 9 三、Micro-Tom番茄與p35S::TDR4、pTDR4::TDR4與TDR4 co-suppression轉殖株中發現PIF1a與TDR4的表現趨勢接近 10 四、於Micro-Tom番茄與轉殖株中不同時期果實的基因表現裡發現PIF1a與TDR4的表現趨勢相近 11 五、對Micro-Tom番茄的果實分別處理藍光與黑暗並收取處理五天後及十四天後的果實進行基因表現量的檢測中發現PIF1a與TDR4的表現趨勢接近 11 六、以Micro-Tom番茄與p35S::TDR4轉殖株的幼苗與果實分別在黑暗及藍光中處理五天後進行基因檢測,發現PIF1a與TDR4的表現趨勢接近 12 七、TDR4重組蛋白質會專一性的結合在PIF1a啟動子的CArG motif區域上 13 八、TDR4在in vitro下與PIF1a有蛋白質之間的交互作用 14 討論 15 一、PIF1a在番茄野生型與轉殖株中之基因表現探討 15 二、藍光處理下TDR4與PIF1a之間的表現探討 16 三、TDR4與PIF1a之間的調控探討 18 總結 19 未來工作建議 20 結果圖片 21 參考文獻 32 附錄 實驗操作流程 38 附圖與附表 42 | |
dc.language.iso | zh-TW | |
dc.title | TDR4與PIF1a在果實紅熟過程中調節茄紅素累積之功能性研究 | zh_TW |
dc.title | Functional studies of TDR4 and PIF1a involved in the regulation of lycopene accumulation in the ripening of tomato fruit | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張英?,王雅筠,楊淑怡,蔡皇龍 | |
dc.subject.keyword | 茄紅素,藍光,TDR4,PIF1a,PSY1, | zh_TW |
dc.subject.keyword | Lycopene,Blue light,TDR4,PIF1a,PSY1, | en |
dc.relation.page | 46 | |
dc.identifier.doi | 10.6342/NTU201802960 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-12 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-107-1.pdf 目前未授權公開取用 | 2.03 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。