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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 李昆達(Kung-Ta Lee) | |
dc.contributor.author | Yu-Jie Lin | en |
dc.contributor.author | 林宇倢 | zh_TW |
dc.date.accessioned | 2021-06-17T04:28:41Z | - |
dc.date.available | 2025-03-05 | |
dc.date.copyright | 2020-03-05 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-02-27 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/70461 | - |
dc.description.abstract | 愈來愈多的植物胜肽分子被發現會參與植物防禦,藉由啟動茉莉酸訊息傳遞路徑,促進次級代謝物生產。然而這些胜肽在毛狀根累積次級代謝物中扮演怎樣的角色仍所知甚少。本研究中我們首先建立番茄毛狀根。接著利用菸草毛狀根作為研究材料,測試多種胜肽對尼古丁含量之影響,並參考菸草毛狀根之操作程序測並測試胜肽對番茄毛狀根累積番茄鹼能力之影響。研究結果顯示,在菸草毛狀根中,0.1 μM茉莉酸甲酯能刺激尼古丁累積,但添加菸草或番茄系統素後,原本0.1 μM茉莉酸甲酯所能累積之番茄鹼含量受到顯著抑制。而在番茄毛狀根中,同時添加0.1 μM茉莉酸甲酯及番茄系統素或flg22,番茄鹼含量相較只有0.1 μM茉莉酸甲酯的處理下降20%。本研究結果顯示,無論在煙草或是番茄毛狀根中,特定胜肽會抑制0.1 μM茉莉酸甲酯累積代謝物之能力,暗示著與0.1 μM 茉莉酸甲酯及特定胜肽間存在著拮抗關係。 | zh_TW |
dc.description.abstract | More and more signaling peptides have been discovered to participate in plant defense through stimulating jasmonate (JA) signaling pathway that activates defense genes and enhances the production of secondary metabolites. However, little is known pertaining to the role of peptides in hairy root cultures accumulating secondary metabolites. In this study, tomato hairy roots were first established. Afterwards, tobacco hairy roots were used as the study model to screen out peptides that could affect nicotine content. Based on the screening procedure established in tobacco hairy roots, a screening procedure for the tomato hairy root targeting its metabolite, tomatine, was subsequently developed. Through this process, we found nicotine content elevated by 0.1 µM MeJA but decreased significantly after the addition of tobacco or tomato systemin. Tomatine content decreased by 20% in comparison with that in 0.1 µM MeJA after the addition of tomato systemin or flg22. These results demonstrate that in both tobacco and tomato hairy root cultures, the addition of specific peptides gave rise to a negative effect of 0.1 µM MeJA on accumulating secondary metabolites, suggesting an antagonistic effect between 0.1 µM MeJA and peptides. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T04:28:41Z (GMT). No. of bitstreams: 1 U0001-2602202014221000.pdf: 3030300 bytes, checksum: b2a3535acc05e08cce6d645210f2ba5a (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | 誌謝 i Abstract ii 中文摘要 iii Chapter 1: Introduction 1 1.1 Hairy root cultures 1 1.1.1 Induction of hairy roots 1 1.1.2 Hairy roots in valuable plant secondary metabolites production 1 1.2 Plant secondary metabolites 2 1.2.1 Classification of plant secondary metabolites 2 1.2.2 Production of therapeutic secondary metabolites using hairy root cultures 3 1.2.3 Alkaloids in Nicotiana tabacum 5 1.2.4 Steroidal glycoalkaloids in Solanum lycopersicum 5 1.3 Role of secondary metabolites in plant defense response 6 1.4 Peptides involved in the defense responses and immunity 7 1.4.1 Perception of danger signals 7 1.4.2 Bacterial flagellin-derived flg22 peptide 7 1.4.3 Endogenous peptides 8 Chapter 2: Materials and Methods 11 2.1 Plant materials 11 2.2 Agrobacterium rhizogenes strain and culture condition 11 2.3 Infection and induction 11 2.4 Tomato hairy root molecular confirmation 12 2.5 Culture condition of hairy roots 13 2.5.1 Nicotiana tabacum hairy roots 13 2.5.2 Solanum lycopersicum hairy roots 14 2.6 Peptide preparation and application 14 2.7 Methyl jasmonate preparation and application 15 2.8 Alkaloids quantification and quantification by UPLC 15 2.8.1 Alkaloids extraction 15 2.8.2 UPLC condition 16 2.8.3 Standard curves for quantification 16 2.9 Glycoalkaloids quantification and quantification by UPLC-QTOF/MS 17 2.9.1 Glycoalkaloids extraction 17 2.9.2 UPLC-QTOF-MS conditions 17 2.9.3 Standard curves for quantification 18 2.10 Statistical analysis 19 Chapter 3: Results 20 3.1 Method development 20 3.2 Effect of flg22 on growth and nicotine content in tobacco hairy roots 21 3.3 Effect of TomSys and TobSys on nicotine content in tobacco hairy roots 21 3.4 Induction and establishment of tomato hairy roots 22 3.5 Confirmation of tomato hairy roots by PCR 23 3.6 Detection of tomatine occurrence in tomato plants and hairy roots 23 3.7 Comparative growth and tomatine production of each hairy root clone 24 3.8 Growth kinetics of LE3 and tomatine accumulation 25 3.9 Effect of Flg22 on tomatine content and growth in tomato hairy roots 25 3.10 Effect of tomato systemin on tomatine content and growth in tomato hairy roots 26 Chapter 4: Discussion 27 4.1 Developments and modifications of the screening procedure 27 4.2 Effect of MeJA on growth and metabolites content in hairy roots 28 4.3 Comparison of the screening results in tobacco and tomato hairy roots 29 4.4 The antagonistic effect between 0.1 µM MeJA and peptides 31 Chapter 5: Conclusion 33 List of Figures 34 Abbreviations 48 References 49 | |
dc.language.iso | en | |
dc.title | 添加胜肽對毛狀根生物鹼含量之影響 | zh_TW |
dc.title | Effect of Peptides on Alkaloids Occurrence in Hairy Root Cultures | en |
dc.type | Thesis | |
dc.date.schoolyear | 109-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 楊健志(Chien-Chih Yang),陳佩燁(Rita P.-Y. Chen),賴爾?(Erh-Min Lai),鄭秋萍(Chiu-Ping Cheng) | |
dc.subject.keyword | 毛狀根,次級代謝物,菸草,番茄,胜?, | zh_TW |
dc.subject.keyword | hairy root,secondary metabolite,Nicotiana tabacum,Solanum lycopersicum,peptide, | en |
dc.relation.page | 52 | |
dc.identifier.doi | 10.6342/NTU202000628 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-02-27 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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