百合防禦相關蛋白LsGRP1誘導植物抗灰黴病之應用

Abstract

百合為具有高經濟價值的球根花卉，除了土壤傳播性病蟲害外，影響其生產最鉅者為Botrytis elliptica (Berk.) Cooke所引起的百合灰黴病，如何有效地防治灰黴病，便成為農友最關心的問題。植物防禦相關蛋白LsGRP1 (Lilium ‘Star Gazer’ glycine-rich protein 1) 為水楊酸誘發葵百合Lilium cv. Star Gazer 對灰黴病抗性過程大量表現的蛋白。根據前人的研究，LsGRP1可能透過直接抑制微生物而抑制病原菌的侵染，本研究則探討LsGRP1是否能透過誘導抗病機制幫助植物抵抗灰黴病菌，並探討其應用價值與最適化之施用條件。首先將大腸桿菌生產的LsGRP1重組蛋白注射於百合中位葉，評估此處理是否能誘發系統葉組織對灰黴病的抗性，並同時測試施用時間、施用濃度及施用位置對LsGRP1重組蛋白誘導植物抗病之影響。接著為降低防治成本，將胞內表現LsGRP1重組蛋白的大腸桿菌以超音波破菌後直接處理植物，測試其誘發系統抗病性的作用，並評估其用以防治其他台灣重要作物病害，如草莓及番茄灰黴病的可行性。期本研究能協助發展新穎的植物病害防治資材，用以保障農作物生產，促進環境永續發展。

Lily is a bulbous flower crop with high economic importance. Beside of soil-borne diseases and pests, gray mold disease caused by Botrytis elliptica, affects lily production enormously. How to effectively control gray mold disease is a main concern of lily farmers. Defense-related protein LsGRP1 (Lilium ‘Star Gazer’ glycine-rich protein 1) is a protein with increased expression in lily which exhibits salicylic acid-induced resistance against gray mold disease. According to previous reports, LsGRP1 may enhance plant immunity via its antimicrobial activity. In this study, the potential of LsGRP1 to induce plant defense against gray mold pathogen and optimal application methods were investigated. At first, LsGRP1-derived recombinant protein produced by Escherichia coli was infiltrated into the middle leaves of lily plants to evaluate its effects on the induction of disease resistance in upper and lower systemic leaves. The time points of application, optimal concentrations, and application sites were determined by comparing the efficacy of disease suppression. In order to reduce the cost, the total lysate of the LsGRP1-derived recombinant protein from sonicated E. coli cells was applied directly to lily plants to examine the effects of systemic resistance induction. On the other hand, the protection effect of crude LsGRP1-derived recombinant protein on the gray mold disease of strawberry and tomato were evaluated. Expectedly, this study would facilitate the development of novel disease control agents suitable for crop production and environmental sustainability.