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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王愛玉 | |
dc.contributor.author | Yung-Yu Chang | en |
dc.contributor.author | 張永郁 | zh_TW |
dc.date.accessioned | 2021-06-17T03:31:37Z | - |
dc.date.available | 2028-02-29 | |
dc.date.copyright | 2018-03-02 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-02-19 | |
dc.identifier.citation | 張皓鈞 (2012) 綠竹中 BoMSP41基因之分子選殖與檢定,碩士論文,國立臺灣大學生命科學院生化科技學系。
楊芳潔 (2013) 綠竹 BoMSP41 之細胞內定為與生化性質探討,碩士論文,國立臺灣大學生命科學院生化科技學系。 李佩儒 (2014) 重組綠竹 Monocot-Specific Protein-41 之純化與性質檢定,碩士論文,國立臺灣大學生命科學院生化科技學系。 黃塏荔 (2015) 綠竹 BoMSP41 轉殖水稻之建立,碩士論文,國立臺灣大學生命科學院生化科技學系。 林延翰 (2016) 綠竹 BoMSP41 與其他分子交互作用之探討,碩士論文,國立臺灣大學生命科學院生化科技學系。 徐暐涵 (2017) 異源表現綠竹 BoMSP41 對於水稻生長及穀粒外觀及品質之影響,碩士論文,國立臺灣大學生命科學院生化科技學系。 An F, Zhang X, Zhu Z, et al., 2012. Coordinated regulation of apical hook development by gibberellins and ethylene in etiolated Arabidopsis seedlings. Cell Res 22, 915-27. Arenas-Huertero F, Arroyo A, Zhou L, Sheen J, Leo´N P, 2000. Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar. Gene Dev 14, 2085-96. Auge GA, Blair LK, Karediya A, Donohue K, 2017. The autonomous flowering-time pathway pleiotropically regulates seed germination in Arabidopsis thaliana. Ann Bot. Ausin I, Alonso-Blanco C, Martinez-Zapater JM, 2005. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69868 | - |
dc.description.abstract | BoMSP41 (Bambusa oldhamii monocot specific protein-41) 為綠竹中未知功能 基因,其基因表現量於綠竹快速生長時期的節間被大量提升,推測其可能參與綠竹生長。本研究欲探討 BoMSP41 之功能,故建立帶有 FLAG-BoMSP41 或 BoMSP41-FLAG 的轉殖阿拉伯芥 (Arabidopsis thaliana),觀察異源表現 BoMSP41 對於轉殖株生長之影響。觀察發現轉殖株相對於野生型的開花時間較晚、株高較高且於高濃度的葡萄糖下,其發芽率較低。推測 BoMSP41 可能參與阿拉伯芥的開花、細胞增生或延長以及種子發芽。 | zh_TW |
dc.description.abstract | BoMSP41 (Bambusa oldhamii monocot specific protein-41) is an unknown function gene which is greatly up-regulated in the internodes of rapid growing bamboo, suggesting that BoMSP41 may involve in bamboo growth. To gain insight into the function of BoMSP41, Arabidopsis transgenic plants overexpressing Flag-BoMSP41 or BoMSP41-FLAG is established. The effect of ectopic expression of BoMSP41 on the transgenic plants was investigated. Comparing to the wild type, the flowering time was delayed and the plant height was increased in the transgenic Arabidopsis. Additionally, the germination rate of the transgenic plant was decreased under high concentration of glucose. The results suggest that BoMSP41 may involve in the flowering, cell proliferation and/or elongation, and seed germination. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T03:31:37Z (GMT). No. of bitstreams: 1 ntu-107-R04b22033-1.pdf: 5351878 bytes, checksum: a9c84cf8ede4de0c7336377d1557e599 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 摘要 1
Abstract 2 縮寫表 3 第一章 緒論 7 第一節 前言 7 1.1 BoMSP41的發現 7 1.2 BoMSP41之生化特性 7 1.3 BoMSP41 之細胞內定位 8 1.4 BoMSP41 於轉殖水稻之功能 8 第二節 研究主題與目的 9 第二章 材料與方法 10 第一節 實驗材料 10 1.1 阿拉伯芥 (Arabidopsis thaliana, Col-0) 10 1.2 菌種 10 1.3 質體 10 1.4 藥品與試劑 10 第二節 實驗方法 11 2.1 轉殖株的建立 11 2.1.1 阿拉伯芥種子消毒與種植 11 2.1.2 阿拉伯芥種植 11 2.1.3 農桿菌轉形 12 2.1.4 花序沾染法 (Clough & Bent, 1998) 12 2.1.5 同型合子篩選 12 2.2 阿拉伯芥的核酸萃取與分析 13 2.2.1 阿拉伯芥染色體DNA之分析 13 2.2.2 阿拉伯芥mRNA之分析 14 2.3 阿拉伯芥的性狀分析 18 2.3.1 轉殖阿拉伯芥之生長指標觀察 18 2.3.2 轉殖阿拉伯芥於植物賀爾蒙處理下的性狀觀察 19 2.3.3 轉殖阿拉伯芥於糖與鹽處理下的性狀觀察 20 2.4 轉殖阿拉伯芥的次細胞定位 21 2.4.1 阿拉伯芥原生質的製備 21 2.4.2 原生質體的轉形 22 2.4.3 於共軛焦顯微鏡之觀察 22 第三章 結果 23 第一節 轉殖株建立與基因插入、mRNA表現分析 23 1.1 阿拉伯芥轉殖株均篩選到獨立品系之同型合子 (Homozygotes) 23 1.2 阿拉伯芥轉殖株確認有 BoMSP41 轉基因插入 23 1.3 MF 和FM 轉殖株具有 BoMSP41 mRNA 基因表現 23 第二節 轉殖株性狀觀察與分析 24 2.1 轉殖阿拉伯芥生長指標分析 24 2.1.1 轉殖阿拉伯芥之開花時間分析 24 2.1.2 轉殖阿拉伯芥植株高度分析 24 2.1.3 轉殖阿拉伯芥果莢型態分析 25 2.1.4 轉殖阿拉伯芥種子型態分析 25 2.2 轉殖阿拉伯芥以植物賀爾蒙處理之性狀觀察 25 2.2.1 GA3 對發芽率及下胚軸延長之影響 25 2.2.2 生長素處理對轉殖株根長之影響 26 2.2.3 細胞分裂素處對下胚軸延長之影響 26 2.3 轉殖阿拉伯芥以糖及鹽處理之性狀觀察 27 2.3.1 糖處理對之影響 27 2.3.2 鹽逆境對發芽率及根延長之影響 27 第三節 次細胞定位 28 第四章 討論與未來展望 29 第一節 阿拉伯芥轉殖株性狀與 BoMSP41 表現之關聯性 29 第二節 BoMSP41 轉殖阿拉伯芥與轉殖水稻之性狀比較 29 第三節 BoMSP41 轉殖阿拉伯芥之細胞內定位 30 第四節 BoMSP41 可能參與之途徑 31 參考文獻 34 圖與表 39 附錄 64 | |
dc.language.iso | zh-TW | |
dc.title | 異源表現綠竹 BoMSP41 對於阿拉伯芥生長之影響 | zh_TW |
dc.title | Effects of ectopic expression of BoMSP41 on the growth of Arabidopsis thaliana | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 楊健志,洪傳揚 | |
dc.subject.keyword | 異源表現,開花,株高,發芽, | zh_TW |
dc.subject.keyword | ectopic expression,flowering,plant height,germination, | en |
dc.relation.page | 65 | |
dc.identifier.doi | 10.6342/NTU201800504 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-02-19 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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