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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 周涵怡 | |
dc.contributor.author | Bo-Ru Chiou | en |
dc.contributor.author | 邱柏儒 | zh_TW |
dc.date.accessioned | 2021-06-17T03:29:23Z | - |
dc.date.available | 2018-03-07 | |
dc.date.copyright | 2018-03-07 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-02-23 | |
dc.identifier.citation | 1. Daneman, D. 'Type 1 diabetes.' The Lancet 367.9513 (2006): 847-858.
2. Banting FG et al “Pancreatic extracts in the treatment of diabetes mellitus: preliminary report.” Canadian Medical Association Journal (1922); 12:141. 3. Carter, Jeffrey D. et al. 'A practical guide to rodent islet isolation and assessment.' Biological procedures online 11.1 (2009): 3. 4. Chiang, Jane L. et al. 'Type 1 diabetes through the life span: a position statement of the American Diabetes Association.' Diabetes care 37.7 (2014): 2034-2054. 5. Clark, S. A. et al. 'Staining and in vitro toxicity of dithizone with canine, porcine, and bovine islets.' Cell transplantation 3.4 (1994): 299-306. 6. Gee, K. R., et al. 'Detection and imaging of zinc secretion from pancreatic β-cells using a new fluorescent zinc indicator.' Journal of the American Chemical Society 124.5 (2002): 776-778. 7. Jiang, Jianjie, et al. 'Generation of insulin‐producing islet‐like clusters from human embryonic stem cells.' Stem cells 25.8 (2007): 1940-1953. journal83.978 (2007): 224-229. 8. Kelly WD et al” Allotransplantation of pancreas and duodenum along with the kidey in diabetic nephropathy.” Surgery (1967);61:827-37. 9. Li, Yang V. 'Zinc and insulin in pancreatic beta-cells.' Endocrine 45.2 (2014): 178-189. 10. Najarian JS et al: Human islet transplantation: a 11. Neuman J. C. et.al “A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination.” JOVE 2014; (88): 50374. 12. Patience, C et al.'Infection of human cells by an endogenous retrovirus of pigs.' Nature medicine 3.3 (1997): 282-286. preliminary report. Transplantation Proceedings (1977);9:233-6. 13. Ronald K. C. et al.. Joslin's Diabetes Mellitus (14th ed.). Lippincott Williams & Wilkins. (2005)ISBN 978-8493531836. 14. Seino, S. et al. 'Dynamics of insulin secretion and the clinical implications for obesity and diabetes.' The Journal f clinical investigation 121.6 (2011): 2118. 15. Shapiro et al. 'International trial of the Edmonton protocol for islet transplantation.' New England Journal of Medicine 355.13 (2006):1318-1330. 16. Shapiro et al. 'Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen.' New England Journal of Medicine 343.4 (2000): 230-238. 17. Srinivasan P. et al. 'Islet cell transplantation.' Postgraduate medical 18. Vijan, S. 'Type 2 diabetes.' Annals of internal medicine 152.5 (2010): ITC3-1. 19. Williams P “Notes on diabetes treated with extract and by grafts of sheep’s pancreas.” British Medical Journal 1894;2:1303-4 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/69819 | - |
dc.description.abstract | 全世界約有四億多人罹患糖尿病,其中10%為甲型糖尿病患,是因自體免疫疾病導致胰島細胞缺損而無法分泌足夠的胰島素;90%則是乙型糖尿病是因細胞對胰島素不敏感的胰島素抗性所導致。目前糖尿病的治療方式之一是大體胰島移植,主要應用在甲型糖尿病患者幫助其重新恢復調控血糖恆定的能力。但目前尚未出現有效的方法控制移植的胰島品質,再加上如血管栓塞等其餘的手術後併發因素,導致有約30%的病人會需要在3個月內重新進行移植手術。因此本研究欲建立一個能夠篩選胰島品質的系統,可望在臨床醫療上對於胰島品質的篩選有所貢獻。本實驗預期利用FluoZin™-3鋅離子螢光染劑,配合鋅離子螯合螢光指示劑以偵測與胰島素結合在一起的鋅離子。藉由以上系統挑選對於葡萄糖刺激有正常分泌反應的健康胰島細胞再予以移植入病患體內以提高手術成功率及預後情形。除了挑選一般胰島品質外,此系統也可應用在挑選自體幹細胞生成之胰島相似細胞簇,可去除胰島移植的排斥作用。但此種治療方式仍在發展階段,利用本系統可篩選出具有正常胰島功能的細胞簇,將有助於此種新興療法發展。 | zh_TW |
dc.description.abstract | Diabetes is a common metabolic disease all over the world. According to statistics from International Diabetes Federation, there is 415 million people with diabetes until 2015. Approximately 90% of diabetes patients are type2 diabetes, while 10% are type 1, which is due to autoimmune problem and result in pancreatic islet cell loss. One of eye-catching therapy to Type 1 diabetes is pancreatic islet transplantation. Although pancreatic islet transplantation become hopeful since it is reported that it can help patients reach insulin independent, it remain quite lots of problems such as shortage of source of islet, low survival rate of transplanted islets and immune risk of allograft. Also, present procedure of islet transplantation does not screen islet function individually, so there might be some functionless tissue transplanted to the recipient unintentional, and that’ll cause more risk to the recipient. So in this study, we tried to establish a rapid functional screen system for islet through detecting insulin cofactor zinc ion instead of detecting insulin itself. In this way we can avoid time consuming step in order to detect protein and high cost of using antibodies. As a result, screening every islets that transplanted to patients become possible. This system also possess the potential to apply in autologous stem cell therapy which is also considered as a promising option to cure type1 diabetes in the future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T03:29:23Z (GMT). No. of bitstreams: 1 ntu-107-R01450014-1.pdf: 2057445 bytes, checksum: 8b6834ee58b88f3b106b8b96f6444e1b (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 致 謝 i
摘 要 ii Abstract iii 目 錄 iv 第一章 導論 1 1.1 糖尿病 1 1.2 成因 2 1.3 治療方式 2 1.4 胰島移植 3 1.5 Edmonton protocol的問世 4 1.6 胰島移植的限制 4 1.7 胰島移植的未來 5 1.8 胰島素的製造 6 1.9 鋅離子與胰島素 7 1.10 研究目的 10 第二章 材料與方法 11 2.1 篩網過濾法小鼠胰島分離 11 2.2 傳統密度梯度離心法小鼠胰島分離 11 2.3 鋅離子濃度偵測 12 2.4 酵素免疫分析法(ELISA) 12 2.5 葡萄糖刺激胰島素分泌實驗 12 2.6 螢光顯微鏡 13 2.7 細胞凋亡檢測 13 第三章 結果 15 3.1 胰島分離方法 15 3.2 不同胰島分離方式的結果 15 3.3 鋅離子與胰島素之對應關聯 16 3.4 葡萄糖刺激下胰島之螢光反應 17 3.5 胰島良率 17 第四章 總結 18 第五章 討論 19 第六章 未來方向 20 第七章 圖表 22 第八章 參考文獻 32 | |
dc.language.iso | zh-TW | |
dc.title | 探討胰島移植中優化分離與篩選之方法 | zh_TW |
dc.title | Study on pancreatic islet isolation and screening methods for improving transplantation therapy | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張百恩,陳敏慧 | |
dc.subject.keyword | 胰島,鋅離子,胰島素,胰島分離,胰島移植,糖尿病,胰島功能, | zh_TW |
dc.subject.keyword | pancreatic islet,zinc ion,insulin,islet isolation,islet transplantation,diabetes mellitus,islet function, | en |
dc.relation.page | 33 | |
dc.identifier.doi | 10.6342/NTU201800645 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-02-23 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
顯示於系所單位: | 口腔生物科學研究所 |
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