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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68831
標題: | 發展週期性結構表面增強拉曼散射基板及其作為DNA探針之應用 Development of periodically structured SERS substrates and its application as DNA probe carrier. |
作者: | Hsiang-Fang Hsieh 謝祥方 |
指導教授: | 管傑雄 |
關鍵字: | 表面增強拉曼散射,拉曼光譜,二維週期性結構基板,電子束微影,DNA探針, Surface Enhanced Raman Scattering,Raman Spectroscopy,Periodically Structured Substrate,Electron-Beam Lithography,DNA probe, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 表面增強拉曼散射是改善拉曼散射強度不足的方法之一,而表面增強拉曼散射之所以能改善的原因有二:利用電磁場增強的方法、與利用電荷轉移增強效應。其中電磁場增強的效果比電荷轉移的效果強上許多,因此許多科學家致力於這方面的研究。若要使電磁場增強,則拉曼的基板製備就會是一個很重要的課題。隨著奈米材料製備技術的成熟,各種基板不斷發展,都以增強表面電漿子共振為主,形貌上多屬於球狀、針尖狀。本論文欲以上述電磁場增強為主,製作一種二維週期性結構基板。設計各式二維銀柱子陣列圖形,其具有調變光的振幅與相位的特性,能藉由調整兩個方向的週期以及銀柱子的尺寸,使不同波數區段的拉曼訊號放大。我們以電子束顯微影系統搭配電子槍蒸鍍金屬技術,製作相同高度但不同尺寸的銀柱子和不同週期排列的陣列圖形基板,並將DNA探針片段連結到此一基板上,去量測所能得到的最佳訊號增強,藉由研究其增強的趨勢,再觀察其圖案之陣列組合,探討此種二維柱子陣列的圖形在怎樣的條件下能對應我們想要增強的DNA探針片段之訊號。 Surface Enhanced Raman Scattering (SERS) is one of the methods to improve the signal intensity of Raman Spectrum. The mechanisms that make SERS improve the signal intensity are the electric field enhancement and the carrier transmission enhancement. According to the fact that electric field effect can provide a stronger enhancement than the chemical effect, numerous scientists commence doing a lot of research on the electric field effect of SERS. If the electric field effect is going to be implemented to enhance the Raman Intensity, solving the problem of the SERS-active substrate is a necessity. As the nano technique matures, various types of SERS-active substrates are fabricated. Nevertheless, most of the substrates, which is designed for the enhancement of surface plasmon effect, are sphere liked, tip liked and metal evaporated. Therefore, this thesis would like to use two-dimensional array as the pattern of our periodically structured SERS substrate. Arrays can modulate the amplitude and the phase of incident light, as the period of the array and the size of the rod has changed, the signal of different wavenumber could be enhanced. We utilized E-beam lithography system with E-beam evaporation system for the fabrication of precise array pattern. After that, a SERS substrate with different period and silver-rod size are obtained. Then we manage to coat DNA fragments on it as the analyte since we wanted to use it as the carrier of DNA probe. By studying and observing the trend of different signals we obtained in different designed pattern, we summed up a conclusion that by altering the periods of two dimensions and the rod size we could enhance the signal in the wanted region of the Raman spectrum. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68831 |
DOI: | 10.6342/NTU201703347 |
全文授權: | 有償授權 |
顯示於系所單位: | 生醫電子與資訊學研究所 |
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