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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李芳仁(Fang-Jen Lee) | |
| dc.contributor.author | Jen-Hao Hu | en |
| dc.contributor.author | 胡振豪 | zh_TW |
| dc.date.accessioned | 2021-06-17T02:37:28Z | - |
| dc.date.available | 2022-09-08 | |
| dc.date.copyright | 2017-09-08 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-08-17 | |
| dc.identifier.citation | Antonny, Bruno, et al. 'N-terminal hydrophobic residues of the G-protein ADP-ribosylation factor-1 insert into membrane phospholipids upon GDP to GTP exchange.' Biochemistry 36.15 (1997): 4675-4684.
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'Affinity purification of Ypt6 effectors and identification of TMF/ARA160 as a Rab6 interactor.' Methods in enzymology 403 (2005): 599-607. Travers, Kevin J., et al. 'Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation.' Cell 101.3 (2000): 249-258. Vetter, Ingrid R., and Alfred Wittinghofer. 'The guanine nucleotide-binding switch in three dimensions.' Science 294.5545 (2001): 1299-1304. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68829 | - |
| dc.description.abstract | ARLs家族蛋白已經被發現在囊泡運輸扮演著重要的腳色，在酵母菌中，Arl1p及下游的Imh1p被認為與Rab家族的Ypt6p是兩條冗餘路徑，許多ypt6Δ的表型都可被高表現量的Arl1p及Imh1p所抑制。在這篇研究中，我們發現Arl1p路徑與Ypt6p路徑藉由GARP complex連結，另外，Imh1p胺基端的結構似乎對與GARP complex的交互作用扮演著相當重要的角色，我們更進一步發現，在野生酵母菌中雖然一般條件下著樣子的交互作用非常微弱，但在內質網壓力下，這樣子的交互作用卻對GARP complex執行核內體到高基氏體的逆向運輸相當重要。我們也發現了一個目前未知功能的高基氏體蛋白：Sft2p同樣參與在Arl1p路徑之中，在研究的最後我們探索了GARP complex作為Arl1p的GEF的可能性。 | zh_TW |
| dc.description.abstract | ARF-like (ARLs) protein have already known to play a role in vesicle trafficking. In yeast, Arl1p and its effector Imh1p is thought to be a redundant pathway with Rab family protein Ypt6p. Various phenotypes in ypt6Δ could be suppress through overexpression of Arl1p and Imh1p. In this study, we find that Arl1p-Imh1p pathway cross talk with Ypt6 pathway through GARP complex. The N-terminal of Imh1 seems plays a important role to interact through GARP complex. Furthermore, we find that although in normal condition such cross talk is very miner, this cross talk through GARP complex is important to against endosome-to-Golgi transport defect under ER stress. We also identify Sft2p, a unknown function protein localize to TGN, also participate in Arl1p-Imh1p pathway. In the end we give a hint that GARP complex subiut Vps53p might be a GEF of Arl1p. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T02:37:28Z (GMT). No. of bitstreams: 1 ntu-106-R04448018-1.pdf: 18918929 bytes, checksum: 87f2fb413dc6dc7950b46e95a95118b2 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
摘要 ii Abstract iii Contents iv Introduction 1 Small GTPase protein 1 ADP-ribosylation factor (Arf) family 2 ADP-ribosylation factor-like (Arl) family 3 Arl1p and Imh1p in yeast 3 Genetic interaction between Arl1-Imh1 pathway and ypt6 pathway. 5 Unfolded protein response(UPR) 6 Materials and Methods 8 Result 17 Overexpression of Arl1 and Imh1 could rescue Snc1 and Sft2 localization in ypt6Δ 17 Overexpression of Arl1 and Imh1 also restores mislocalization of GARP complex in ypt6Δ. 18 Arl1 and Imh1 rescuing Snc1 and Sft2 localization require GARP complex subunits. 19 Sft2p also participates in Arl1 pathway to rescue Snc1p localization in ypt6Δ. 20 Sft2 don’t affect the restoring of Imh1 or GARP complex localization in ypt6Δ by overexpressing Arl1. 21 Arl1p and Imh1p are required for Vps53p localization while tunicamycin treatment. 22 Imh1 N-terminal is important for Vps53p localization while Tunicamycin treatment. 23 Vps53p interact with inactive form of Arl1p both in vivo and in vitro but not active form of Arl1p. 24 Overexpression of Vps53 partially rescue ypt6Δ through Arl1p 26 Disruption of Vps53p affect localization of Arl1 and Imh1 27 Discussion 29 The genetic interaction between Arl1-Imh1 pathway and Ypt6 pathway is through GARP complex 29 The function of Sft2p in Arl1-Imh1 pathway. 30 The function of Arl1p and Imh1p in ER stresss 31 Whether subunit of GARP complex, a tethering factor, Vps53p could be a GEF of Arl1? 31 Tables 33 Table 1. Yeast strains used in this study 33 Table 2. 36 Figures 38 Figure 1. Overexpression of Arl1 and Imh1 could rescue Snc1p and Sft2p localization in ypt6Δ. 38 Figure 2. Overexpression of Arl1 and Imh1 also restores mislocalization of GARP complex in ypt6Δ. 40 Figure 3. Arl1 and Imh1 rescuing Snc1p and Sft2p localization require GARP complex subunits. 42 Figure 4. Sft2p also participates in Arl1 pathway to rescue Snc1p localization in ypt6Δ. 44 Figure 5. Sft2 don’t affect the restoring of Imh1 or GARP complex localization in ypt6Δ by overexpressing Arl1. 45 Figure 6. Model of endosome-to-Golgi retrograde trafficking rescuing ability of Arl1 and Imh1 in ypt6Δ. 46 Figure 7. Arl1p and Imh1p are required for Vps53p localization while tunicamycin treatment. 47 Figure 8. Imh1 N-terminal is important for Vps53p localization while Tunicamycin treatment. 48 Figure 9. Model of Arl1-Imh1 pathway in UPR response. 49 Figure 10. Vps53p interact with inactive form of Arl1p both in vivo and in vitro but not active form of Arl1p. 51 Figure 11. Overexpression of Vps53 partially rescue ypt6Δ through Arl1p 53 Figure 12. Disruption of Vps53p affect localization of Arl1 and Imh1. 55 Reference 56 | |
| dc.language.iso | en | |
| dc.subject | Arl1p | zh_TW |
| dc.subject | Imh1p | zh_TW |
| dc.subject | 高基氏體 | zh_TW |
| dc.subject | 逆向運輸 | zh_TW |
| dc.subject | 內質網壓力 | zh_TW |
| dc.subject | Arl1p | zh_TW |
| dc.subject | Imh1p | zh_TW |
| dc.subject | 高基氏體 | zh_TW |
| dc.subject | 逆向運輸 | zh_TW |
| dc.subject | 內質網壓力 | zh_TW |
| dc.subject | ER stress | en |
| dc.subject | Arl1p | en |
| dc.subject | Imh1p | en |
| dc.subject | Arl1p | en |
| dc.subject | Imh1p | en |
| dc.subject | GARP complex | en |
| dc.subject | Sft2p | en |
| dc.subject | ER stress | en |
| dc.subject | Sft2p | en |
| dc.subject | GARP complex | en |
| dc.title | 酵母菌第一腺嘌呤核苷二磷酸核醣化因子相似蛋白與Ypt6p相關路徑之功能性探討 | zh_TW |
| dc.title | Characterization of downstream effector of Ypt6p-dependent Arl1p pathway in yeast | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鄧述諄(Shu-Chun Teng),林敬哲(Jing-Jer Lin),王昭雯(Chao-Wen Wang) | |
| dc.subject.keyword | Arl1p,Imh1p,高基氏體,逆向運輸,內質網壓力, | zh_TW |
| dc.subject.keyword | Arl1p,Imh1p,GARP complex,Sft2p,ER stress, | en |
| dc.relation.page | 59 | |
| dc.identifier.doi | 10.6342/NTU201703859 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-08-17 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 分子醫學研究所 | zh_TW |
| 顯示於系所單位： | 分子醫學研究所 | |
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