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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳克強(Keqiang Wu) | |
| dc.contributor.author | Hua-Chung Sun | en |
| dc.contributor.author | 孫華駿 | zh_TW |
| dc.date.accessioned | 2021-06-17T02:12:03Z | - |
| dc.date.available | 2023-01-29 | |
| dc.date.copyright | 2018-01-29 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2018-01-03 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68065 | - |
| dc.description.abstract | 可逆的組蛋白修飾在調控染色質結構與基因的表達扮演很重要的角色。阿拉伯芥組蛋白去乙醯基酶HDA6參與控制轉座子 (transposable elements) 的靜默。HDA6會與組蛋白H3賴氨酸9 (H3K9)甲基轉移酶KYP交互作用，共同抑制轉座子的表達。此外，HDA6也可以與組蛋白H3賴氨酸4 (H3K4)去甲基轉移酶FLD交互作用，抑制開花抑制子FLC、MAF4和MAF5的表達。我們發現阿拉伯芥JmjC去甲基酶JMJ28有去除組蛋白H3K9Me的活性。在hda6突變體中大量表達的轉座子在jmj28 hda6雙突變體中明顯的受到抑制，說明JMJ28參與調控轉座子的表達調控。進一步研究發現這幾個轉座子的E-box區域上的組蛋白H3K9me2有增加。另外，我們還發現JMJ28可以與HDA6交互作用。此外，jmj28 hda6雙突變體的開花時間明顯晚於jmj28和hda6單突變體，並且在jmj28 hda6雙突變體中FLC、MAF4和MAF5的表達也明顯高於單突變體。這些結果說明雖然JMJ28與HDA6在轉座子的調控中扮演相反的角色，卻可能共同調控阿拉伯芥的開花時間。 | zh_TW |
| dc.description.abstract | Reversible histone modifications play a fundamental role in regulating chromatin structure and gene expression. Previous studies indicated that the histone deacetylase HDA6 is required for the silencing of transposable elements in Arabiopsis thaliana. HDA6 interacts with the H3K9 methyltransferases KYP and co-represses a subset of transposons through H3 deacetylation and H3K9 methylation. Moreover, HDA6 regulates flowering time by interacting with the H3K4 demethylase FLD through repressing the expression of FLC, MAF4 and MAF5. In this study, we showed that the jmjC domain-containing histone demethylase JMJ28 had demethylase activity specific to H3K9me2. A subset of transposons that were highly expressed in hda6 were silenced in the jmj28 hda6 double mutant, indicating that JMJ28 is required for transposon activation. H3K9me2 was enriched at the genomic regions containing the E-boxes in transposons in jmj28. Furthermore, JMJ28 can interact with HDA6. The jmj28 hda6 double mutant flowered later than both the jmj28 and hda6 single mutants. The expression of FLC, MAF4 and MAF5 in the jmj28 hda6 double mutant was also increased compared to the single mutants. Taken together, these results indicate that although JMJ28 and HDA6 play opposite roles in regulation of transposon silencing, they may act synergistically in the regulation of flowering time. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T02:12:03Z (GMT). No. of bitstreams: 1 ntu-106-R04b42024-1.pdf: 1542252 bytes, checksum: b89c0bc0d8bfb15499714fe3a6578f0b (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 誌謝 ii
中文摘要 iii ABSTRACT iv CONTENTS v LIST OF FIGURES viii LIST OF SUPPLEMENTARY FIGURES ix LIST OF TABLES x LIST OF ABBRIVIATIONS xi Chapter 1 Introduction 1 1.1 Histone methylation 1 1.2 Functions of histone demethylases 2 1.3 Transposon repression 4 1.4 Transposon activation 5 Chapter 2 Materials and Methods 7 2.1 Plant materials 7 2.2 Quick DNA extraction (Richard et al., 1994) 7 2.3 RNA isolation 8 2.4 DNase treatment 9 2.5 Quantitative RT-PCR analysis 9 2.6 Chromatin immunoprecipitation assays 10 2.6.1 Tissue Harvesting and formaldehyde cross-linking 10 2.6.2 Immunoprecipitation of chromatin 13 2.6.3 DNA precipitation 16 2.6.4 Quantitative ChIP PCR 17 2.7 Bimolecular Fluorescence Complementation (BiFC) assays 18 2.7.1 Plasmid construction 18 2.7.2 Protoplast isolation 18 2.7.3 Protoplast PEG transfection 20 2.8 Transfection of tobacco leaves by Agrobacterium for BiFC assays 21 2.9 Histone demethylase assays 22 Chapter 3 Results 26 3.1 JMJ28 is localized in the nucleus 26 3.2 JMJ28 interacts with FBH1/2, HDA6, KYP and FLD 26 3.3 JMJ28 has histone H3K9me2 demethylase activity 27 3.4 JMJ28 is required for transposon activation in the hda6 mutant. 28 3.5 JMJ28 decreases the level of H3K9me2 in transposons. 29 3.6 JMJ28 mutants display delayed flowering phenotypes under long day and short day conditions. 29 3.7 The expression of FLC, MAF4 and MAF5 is increased in JMJ28 mutants 30 3.8 The jmj28-1 hda6-6 double mutant flowers later than both jmj28-1 and hda6 single mutants 30 Chapter 4 Discussion 31 4.1 JMJ28 is a H3K9me2 demethylase and interacts with FBH transcription factors 31 4.2 JMJ28 regulates transposon activation 32 4.3 JMJ28 interacts with HDA6 and regulates flowering in Arabidopsis 33 FIGURES 36 SUPPLEMENTARY FIGURES 50 TABLES 53 REFERENCES 57 | |
| dc.language.iso | en | |
| dc.subject | 組蛋白去乙醯?HDA6 | zh_TW |
| dc.subject | 開花時間 | zh_TW |
| dc.subject | 轉座子 | zh_TW |
| dc.subject | 組蛋白去甲基化?JMJ28 | zh_TW |
| dc.subject | 組蛋白修飾 | zh_TW |
| dc.subject | FLC | en |
| dc.subject | flowering time | en |
| dc.subject | Histone modifications | en |
| dc.subject | HDA6 | en |
| dc.subject | JMJ28 | en |
| dc.subject | transposable elements | en |
| dc.title | 阿拉伯芥組蛋白去甲基化酶JMJ28參與開花調控與轉座子的靜默 | zh_TW |
| dc.title | The histone demethylase JMJ28 controls flowering time and transposon silencing in Arabidopsis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 羅?升(Wan-Sheng Lo),鄭秋萍(Chiu-Ping Cheng),鄭貽生(Yi-Sheng Cheng),謝旭亮(Hsu-Liang Hsieh) | |
| dc.subject.keyword | 組蛋白修飾,組蛋白去乙醯?HDA6,組蛋白去甲基化?JMJ28,轉座子,開花時間, | zh_TW |
| dc.subject.keyword | Histone modifications,HDA6,JMJ28,FLC,transposable elements,flowering time, | en |
| dc.relation.page | 64 | |
| dc.identifier.doi | 10.6342/NTU201800004 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-01-03 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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