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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄧麗珍(Lee-Jene Teng) | |
dc.contributor.author | Yu-Jung Liu | en |
dc.contributor.author | 劉又榕 | zh_TW |
dc.date.accessioned | 2021-06-17T01:37:40Z | - |
dc.date.available | 2017-09-12 | |
dc.date.copyright | 2017-09-12 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-07-31 | |
dc.identifier.citation | 參考文獻
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Int J Med Microbiol, 2015. 305(1): p. 110-3. 38. Ferreira, J.P., et al., Transmission of MRSA between companion animals and infected human patients presenting to outpatient medical care facilities. PLoS One, 2011. 6(11): p. e26978. 39. Vandendriessche, S., et al., Methicillin-susceptible Staphylococcus aureus ST398-t571 harbouring the macrolide-lincosamide-streptogramin B resistance gene erm(T) in Belgian hospitals. J Antimicrob Chemother, 2011. 66(11): p. 2455-9. 40. McCarthy, A.J., et al., Staphylococcus aureus CC398 clade associated with human-to-human transmission. Appl Environ Microbiol, 2012. 78(24): p. 8845-8. 41. McCarthy, A.J., et al., Extensive horizontal gene transfer during Staphylococcus aureus co-colonization in vivo. Genome Biol Evol, 2014. 6(10): p. 2697-708. 42. Moon, D.C., et al., Identification of livestock-associated methicillin-resistant Staphylococcus aureus isolates in Korea and molecular comparison between isolates from animal carcasses and slaughterhouse workers. Foodborne Pathog Dis, 2015. 12(4): p. 327-34. 43. Petinaki, E. and I. Spiliopoulou, Methicillin-resistant Staphylococcus aureus among companion and food-chain animals: impact of human contacts. Clin Microbiol Infect, 2012. 18(7): p. 626-34. 44. Wang, X.L., et al., Phenotypic and molecular characteristics of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in slaughterhouse pig-related workers and control workers in Guangdong Province, China. Epidemiol Infect, 2017. 145(9): p. 1843-1851. 45. Wendlandt, S., et al., Multidrug resistance genes in staphylococci from animals that confer resistance to critically and highly important antimicrobial agents in human medicine. Trends Microbiol, 2015. 23(1): p. 44-54. 46. Cuny, C., et al., Methicillin susceptible Staphylococcus aureus (MSSA) of clonal complex CC398, t571 from infections in humans are still rare in Germany. PLoS One, 2013. 8(12): p. e83165. 47. Fetsch, A., et al., Turkey Meat as Source of CC9/CC398 Methicillin-Resistant Staphylococcus aureus in Humans? Clin Infect Dis, 2017. 64(1): p. 102-103. 48. Moodley, A., F. Latronico, and L. Guardabassi, Experimental colonization of pigs with methicillin-resistant Staphylococcus aureus (MRSA): insights into the colonization and transmission of livestock-associated MRSA. Epidemiol Infect, 2011. 139(10): p. 1594-600. 49. Smith, T.C., Livestock-associated Staphylococcus aureus: the United States experience. PLoS Pathog, 2015. 11(2): p. e1004564. 50. Gomez-Sanz, E., et al., Novel erm(T)-carrying multiresistance plasmids from porcine and human isolates of methicillin-resistant Staphylococcus aureus ST398 that also harbor cadmium and copper resistance determinants. Antimicrob Agents Chemother, 2013. 57(7): p. 3275-82. 51. Li, M., et al., Inducible Expression of both ermB and ermT Conferred High Macrolide Resistance in Streptococcus gallolyticus subsp. pasteurianus Isolates in China. Int J Mol Sci, 2016. 17(10). 52. de Vries, L.E., et al., The gut as reservoir of antibiotic resistance: microbial diversity of tetracycline resistance in mother and infant. PLoS One, 2011. 6(6): p. e21644. 53. Tsai, J.C., et al., The erm(T) gene is flanked by IS1216V in inducible erythromycin-resistant Streptococcus gallolyticus subsp. pasteurianus. Antimicrob Agents Chemother, 2005. 49(10): p. 4347-50. 54. Gomez-Sanz, E., et al., Chromosomal integration of the novel plasmid pUR3912 from methicillin-susceptible Staphylococcus aureus ST398 of human origin. Clin Microbiol Infect, 2013. 19(11): p. E519-22. 55. Kadlec, K. and S. Schwarz, Identification of a plasmid-borne resistance gene cluster comprising the resistance genes erm(T), dfrK, and tet(L) in a porcine methicillin-resistant Staphylococcus aureus ST398 strain. Antimicrob Agents Chemother, 2010. 54(2): p. 915-8. 56. Li, G., et al., Staphylococcus aureus ST6-t701 isolates from food-poisoning outbreaks (2006-2013) in Xi'an, China. Foodborne Pathog Dis, 2015. 12(3): p. 203-6. 57. Uhlemann, A.C., et al., Identification of a highly transmissible animal-independent Staphylococcus aureus ST398 clone with distinct genomic and cell adhesion properties. MBio, 2012. 3(2). 58. Li, G., et al., Prevalence and characterization of methicillin susceptible Staphylococcus aureus ST398 isolates from retail foods. Int J Food Microbiol, 2015. 196: p. 94-7. 59. Brennan, G.I., et al., The Emergence and Spread of Multiple Livestock-Associated Clonal Complex 398 Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Strains among Animals and Humans in the Republic of Ireland, 2010-2014. PLoS One, 2016. 11(2): p. e0149396. 60. Sousa, M., et al., Genetic Diversity and Antibiotic Resistance Among Coagulase-Negative Staphylococci Recovered from Birds of Prey in Portugal. Microb Drug Resist, 2016. 22(8): p. 727-730. 61. Messina, J.A., et al., Impact of Bacterial and Human Genetic Variation on Staphylococcus aureus Infections. PLoS Pathog, 2016. 12(1): p. e1005330. 62. McCarthy, A.J., A.A. Witney, and J.A. Lindsay, Staphylococcus aureus temperate bacteriophage: carriage and horizontal gene transfer is lineage associated. Front Cell Infect Microbiol, 2012. 2: p. 6. 63. Dipersio, L.P. and J.R. Dipersio, Identification of an erm(T) gene in strains of inducibly clindamycin-resistant group B Streptococcus. Diagn Microbiol Infect Dis, 2007. 57(2): p. 189-93. 64. Palmieri, C., P.E. Varaldo, and B. Facinelli, Streptococcus suis, an Emerging Drug-Resistant Animal and Human Pathogen. Front Microbiol, 2011. 2: p. 235. 65. Li, Q., et al., Co-addition of manure increases the dissipation rates of tylosin A and the numbers of resistance genes in laboratory incubation experiments. Sci Total Environ, 2015. 527-528: p. 126-34. 66. Yang, S.S., et al., Co-location of the erm(T) gene and blaROB-1 gene on a small plasmid in Haemophilus parasuis of pig origin. J Antimicrob Chemother, 2013. 68(8): p. 1930-2. 67. Luthje, P. and S. Schwarz, Antimicrobial resistance of coagulase-negative staphylococci from bovine subclinical mastitis with particular reference to macrolide-lincosamide resistance phenotypes and genotypes. J Antimicrob Chemother, 2006. 57(5): p. 966-9. 68. Szczuka, E., L. Jablonska, and A. Kaznowski, Coagulase-negative staphylococci: pathogenesis, occurrence of antibiotic resistance genes and in vitro effects of antimicrobial agents on biofilm-growing bacteria. J Med Microbiol, 2016. 65(12): p. 1405-1413. 69. Gatermann, S.G., T. Koschinski, and S. Friedrich, Distribution and expression of macrolide resistance genes in coagulase-negative staphylococci. Clin Microbiol Infect, 2007. 13(8): p. 777-81. 70. Argudin, M.A., et al., Bacteria from Animals as a Pool of Antimicrobial Resistance Genes. Antibiotics (Basel), 2017. 6(2). 71. 萬采玟,甲氧西林敏感金黃色葡萄球菌之抗紅黴素基因結構分析. 國立台灣大學,2014年 72. 鄭耀瑜,凝固酶陰性葡萄球菌之抗紅黴素基因分析. 國立台灣大學,2016年 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/67560 | - |
dc.description.abstract | 金黃色葡萄球菌為臨床上常見的病原菌,其抗藥一直是嚴重的問題。紅黴素為臨床上治療methicillin susceptible Staphylococcus aureus (MSSA)的藥物,過去研究發現在台灣21間醫院抗紅黴素MSSA 比例(161株)佔35%,所以仍有一定的重要性。此外,MSSA可藉由mobile genetic elements (MGE) 傳遞 erm 基因給methicillin resistant S. aureus (MRSA)。因此本研究探討MRSA及MSSA抗紅黴素基因之流行病學分布及其基因結構。菌株為來自台大醫院2013至2015年臨床檢體分離具紅黴素抗性之金黃色葡萄球菌MSSA及MRSA,血液培養分離菌株包括MRSA 286株及MSSA 59株。紅黴素抗藥基因分析顯示286株來自血液MRSA以攜帶ermA基因為最大宗共有111株(39%),而 MSSA 以攜帶msrA/B基因最多,在59株中共測得25株。較特別的是2015年MSSA血液檢體發現有一株帶有ermT基因,ermT基因在其他研究曾指出跟 livestock-associated MRSA (LA-MRSA)及LA-MSSA有關,換句話說,此基因可能在動物與人之間傳遞。分析其spa type及MLST之sequence type,結果顯示此菌株屬於t1379及ST834。其中ST834屬於 (clonal complex) CC9,在過去文獻提到CC9跟亞洲家畜相關金黃葡萄球菌有關,而本篇為第一個發現MSSA ST834-CC9菌株攜帶ermT基因。以PCR mapping與southern blot確認此ermT附近基因結構與已知攜帶ermT基因的MSSA NTUH-8300結構不同。此外,也分析CoNS (NTUH-4483, NTUH-2381-1) 與S. aureus (NTUH-154, pUR3912) 攜帶ermT基因結構,在此篇研究發現不同物種其ermT基因周圍結構有些不一樣。為了更探討台大醫院其他檢體中是否有攜帶ermT的菌株,擴大檢體收集範圍並篩選自2016年4月到6月具紅黴素抗性的非血液檢體,結果並無發現攜帶ermT基因,原因推測為收集時間太短的關係。 | zh_TW |
dc.description.abstract | Staphylococcus aureus is a major human pathogen that causes a board range of clinical infections. The use of various antibiotics over the years has led to evolution of multidrug-resistant strains. Erythromycin have important therapeutic roles in methicillin susceptible Staphylococcus aureus (MSSA). Erythromycin-resistant MSSA was collected in 2000 from 21 hospitals in Taiwan. Of the 161 MSSA isolates, 56 (35%) were resistant to erythromycin. As far as we know, erm gene can be transferred to methicillin resistant S. aureus (MRSA) by mobile genetic elements, which is usually carried by MSSA. Thus, the present study demonstrated information on the prevalence of erythromycin-resistance and molecular epidemiology of MSSA and MRSA in National Taiwan University Hosipital. A total of 345 blood isolates of erythromycin-resistant MRSA (286) and MSSA (59) strains were obtained from 2013 to 2015. The prevalence of ermA gene was 39% among MRSA and msrA/B gene was detected in MSSA predominantly. In this study, we found a strain, NTUH-154, which was an MSSA isolate of blood culture that carried ermT gene. The ermT gene has been detected in LA-MRSA and LA-MSSA many times. Besides, ermT gene has also been concerned with cross-species transmission, that is, animal to human. NTUH-154 was subjected to spa typing and multilocus sequence type and the results showed that NTUH-154 belonged to t1379 and ST834 which was classified as CC9. CC9 has been referred to as the most pandemic LA-MRSA and LA-MSSA in most Asian countries. This is the first report of the ermT gene in a ST834-CC9 MSSA. The structure of chromosomal ermT gene in ST834-CC9 NTUH-154 was demonstracted by PCR mapping followed by sequencing and was different from the previous isolate of ST398-CC398 NTUH-8300. On the other hand, we also analyzed the isolates from CoNS that carried the ermT gene. This study also compared the genetic environment of ermT gene between S. aureus (NTUH-154, pUR3912) and CoNS (NTUH-4483, NTUH-2381-1). We found out that different species harbore different combination of genetic environment of ermT gene. Erythromycin-resistant S. aureus non-blood culture isolates were collected during Apr-Jun in 2016. None of these isolates carried ermT gene and this may be due to short period of specimen collection. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T01:37:40Z (GMT). No. of bitstreams: 1 ntu-106-R04424023-1.pdf: 2806220 bytes, checksum: 20bd048966bef3dd6d9ca13ca22068b5 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 總 目 次
致謝 I 中文摘要 II 英文摘要 III 第一章 前言 1 1.1金黃色葡萄球菌簡介 1 1.2金黃色葡萄球菌在台灣抗藥的概況 2 1.3凝固酶陰性葡萄球菌簡介 2 1.4凝固酶陰性葡萄球菌的抗藥情形概述 3 1.5抗生素藥物簡介以及菌株產生抗藥機制之概述 3 1.6 Macrolide-Lincosamide-Streptogramin的抗藥機制 3 1.7 erm基因概述及攜帶方式 4 1.8 ermT基因攜帶攜帶方式 5 第二章 材料與方法 6 2.1臨床分離菌株與文獻實驗用的參考菌株 6 2.2萃取細菌染色體核酸 (bacteria DNA extraction) 6 2.3萃取質體核酸 (plasmid DNA) 8 2.4以PCR鑑定金黃色葡萄球菌 9 2.5以PCR檢驗金黃色葡萄球菌所攜帶的抗紅黴素基因 10 2.6南方墨點法 (southern blot) 11 2.7抗生素最小抑菌濃度 (minimum inhibitory concentration,MIC) 15 2.8抗生素紙錠擴散試驗- (double disk diffusion) 鑑定iMLSB、cMLSB 與M表現型 15 2.9菌株spa type分析 16 2.10菌株MLST 分析 17 2.11 PFGE (Pulsed-field gel electrophoresis) 分析菌株親緣關係 18 2.12 S1 nuclease-PFGE (S1 nuclease-pulsed field gel electrophoresis) 20 2.13 Inverse PCR 21 2.14 dnaJ gene PCR-RFLP (陰性凝固沒葡萄球菌之菌種鑑定) 23 2.15 primer列表 24 第三章 結果 29 3.1 篩選實驗菌株 29 3.2凝固酶陰性菌株鑑定 29 3.3抗紅黴素基因在金黃色葡萄球菌分佈的情形 29 3.4 double disk diffusion分析菌株抗藥表現型 30 3.5以agar dilution 確認紅黴素MIC值 31 3.6 PCR分析金黃色葡萄球菌是否攜帶ermT基因 32 3.7攜帶ermT菌株之spa type分析 32 3.8攜帶ermT菌株之MLST (Multilocus sequence typing) 分析 33 3.9 Southern blot確認菌株攜帶ermT基因 33 3.10 PCR mapping 分析金黃色葡萄萄球菌之ermT基因的結構 33 3.11 Inverse PCR分析金黃色葡萄萄球菌之ermT基因的結構 34 3.12 PCR mapping 分析凝固酶陰性葡萄萄球菌之ermT基因的結構 34 3.13 S1-PFGE分析陰性凝固酶金黃色葡萄球菌質體大小 36 3.14 Southern blot確認陰性凝固酶金黃色葡萄球菌之ermT基因結構 36 3.15 S1-PFGE 轉漬Southern blot 分析 36 3.16 重新設計CoNS PCR mapping primer 36 第四章 討論 38 4.1金黃色葡萄球菌之抗紅黴素抗藥基因與抗藥表現型分析 38 4.2 MRSA與MSSA基因型與抗藥表現型之間的關聯 38 4.3 S1 PFGE轉漬southern blot 39 4.4 CoNS PCR mapping 之primer設計 39 4.5 MRSA 非血液檢體NTUH-NBR 12 之t899-ST9-CC9 40 4.6不同菌株之ermT基因結構比較 41 4.7 ermT基因在金黃色葡萄球菌的分布情形 43 4.8 ermT基因的重要性 44 4.9 ermT 基因與ermC基因 45 第五章 附圖 47 第六章 附表 59 第七章 參考文獻 65 | |
dc.language.iso | zh-TW | |
dc.title | 金黃色葡萄球菌之抗紅黴素基因結構分析 | zh_TW |
dc.title | Erythromycin resistance genes in Staphylococcus aureus | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 廖淑珍(Shwu-Jen Liaw),洪薇鈞(Hung Wei-Chun) | |
dc.subject.keyword | MSSA,MRSA,ermT,LA-MSSA,CC9,ST834,CoNS, | zh_TW |
dc.relation.page | 70 | |
dc.identifier.doi | 10.6342/NTU201701732 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-07-31 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 醫學檢驗暨生物技術學研究所 | zh_TW |
顯示於系所單位: | 醫學檢驗暨生物技術學系 |
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