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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝文陽 | |
dc.contributor.author | Yen-ting Chen | en |
dc.contributor.author | 陳彥婷 | zh_TW |
dc.date.accessioned | 2021-06-17T01:29:45Z | - |
dc.date.available | 2019-08-10 | |
dc.date.copyright | 2017-08-10 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-08-04 | |
dc.identifier.citation | Antunes, A., França, L., Rainey, F. A., Huber, R., Nobre, M. F., Edwards, K. J., and da Costa, M. S. (2007). Marinobacter salsuginis sp. nov., isolated from the brine–seawater interface of the Shaban Deep, Red Sea. International Journal of Systematic and Evolutionary Microbiology, 57, 1035-1040.
Arahal, D. R., Castillo, A. M., Ludwig, W., Schleifer, K. H., and Ventosa, A. (2002). Proposal of Cobetia marina gen. nov., comb. nov., within the Family Halomonadaceae, to include the species Halomonas marina. Systematic and Applied Microbiology, 25, 207-211. Arahal, D. R., García, M. T., Vargas, C., Cánovas, D., Nieto, J. J., and Ventosa, A. (2001). Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174. International Journal of Systematic and Evolutionary Microbiology, 51, 1457-1462. Bagwell, C. E., La Rocque, J. R., Smith, G. W., Polson, S. W., Friez, M. J., Longshore, J. W., and Lovell, C. R. (2002). Molecular diversity of diazotrophs in oligotrophic tropical seagrass bed communities. FEMS Microbiology Ecology, 39, 113-119. Bombar, D., Paerl, R. W., and Riemann, L. (2016). Marine Non-Cyanobacterial Diazotrophs: Moving beyond Molecular Detection. Trends in Microbiology, 24, 916-927. Bowman, J. P. (1998). Pseudoalteromonas prydzensis sp. nov., a psychrotrophic, halotolerant bacterium from Antarctic sea ice. International Journal of Systematic and Evolutionary Microbiology, 48, 1037-1041. Chang, H.-W., Roh, S. W., Kim, K.-H., Nam, Y.-D., Jeon, C. O., Oh, H.-M., and Bae, J.-W. (2008). Shewanella basaltis sp. nov., a marine bacterium isolated from black sand. International Journal of Systematic and Evolutionary Microbiology, 58, 1907-1910. Cherkasov, N., Ibhadon, A. O., and Fitzpatrick, P. (2015). A review of the existing and alternative methods for greener nitrogen fixation. Chemical Engineering and Processing: Process Intensification, 90, 24-33. Chimetto, L. A., Cleenwerck, I., Brocchi, M., Willems, A., De Vos, P., and Thompson, F. L. (2011). Marinomonas brasilensis sp. nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis. International Journal of Systematic and Evolutionary Microbiology, 61, 1170-1175. Choi, D. H., and Cho, B. C. (2005). Idiomarina seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea. International Journal of Systematic and Evolutionary Microbiology, 55, 379-383. Colwell, R. R., and Ceccarelli, D. (2014). Vibrio ecology, pathogenesis and evolution: Frontiers E-books. Crapart, S., Fardeau, M.-L., Cayol, J.-L., Thomas, P., Sery, C., Ollivier, B., and Combet-Blanc, Y. (2007). Exiguobacterium profundum sp. nov., a moderately thermophilic, lactic acid-producing bacterium isolated from a deep-sea hydrothermal vent. International Journal of Systematic and Evolutionary Microbiology, 57, 287-292. Dai, X., Shi, X., Gao, X., Liang, J., and Zhang, X.-H. (2015). Salipiger nanhaiensis sp. nov., a bacterium isolated from deep sea water. International Journal of Systematic and Evolutionary Microbiology, 65, 1122-1126. Dashti, N., Ali, N., Eliyas, M., Khanafer, M., Sorkhoh, N. A., and Radwan, S. S. (2015). Most Hydrocarbonoclastic Bacteria in the Total Environment are Diazotrophic, which Highlights Their Value in the Bioremediation of Hydrocarbon Contaminants. Microbes and Environments, 30, 70-75. de Boer, W. F. (2007). Seagrass–sediment interactions, positive feedbacks and critical thresholds for occurrence: a review. Hydrobiologia, 591, 5-24. de Bruijn, F. J. (2015). Biological nitrogen gixation. In B. Lugtenberg (Ed.), Principles of Plant-Microbe Interactions: Microbes for Sustainable Agriculture (pp. 215-224). Cham: Springer International Publishing. Donachie, S. P., Bowman, J. P., and Alam, M. (2006). Nesiotobacter exalbescens gen. nov., sp. nov., a moderately thermophilic alphaproteobacterium from an Hawaiian hypersaline lake. International Journal of Systematic and Evolutionary Microbiology, 56, 563-567. Foesel, B. U., Drake, H. L., and Schramm, A. (2011). Defluviimonas denitrificans gen. nov., sp. nov., and Pararhodobacter aggregans gen. nov., sp. nov., non-phototrophic Rhodobacteraceae from the biofilter of a marine aquaculture. Systematic and Applied Microbiology, 34, 498-502. Foght, J. (2010). Nitrogen fixation and hydrocarbon-oxidizing bacteria. in K. N. Timmis (Ed.), Handbook of Hydrocarbon and Lipid Microbiology (pp. 1661-1668). Berlin, Heidelberg: Springer Berlin Heidelberg. Gaby, J. C., and Buckley, D. H. (2011). A global census of nitrogenase diversity. Environmental Microbiology, 13, 1790-1799. Gaby, J. C., and Buckley, D. H. (2012). A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase. PLOS ONE, 7, e42149. Gaby, J. C., and Buckley, D. H. (2014). A comprehensive aligned nifH gene database: a multipurpose tool for studies of nitrogen-fixing bacteria. Database: The Journal of Biological Databases and Curation, 2014, bau001. Galloway, J. N., Townsend, A. R., Erisman, J. W., Bekunda, M., Cai, Z., Freney, J. R., Martinelli, L. A., Seitzinger, S. P., and Sutton, M. A. (2008). Transformation of the Nitrogen Cycle: Recent Trends, Questions, and Potential Solutions. Science, 320, 889-892. Gao, Z.-M., Xiao, J., Wang, X.-N., Ruan, L.-W., Chen, X.-L., andZhang, Y.-Z. (2012). Vibrio xiamenensis sp. nov., a cellulase-producing bacterium isolated from mangrove soil. International Journal of Systematic and Evolutionary Microbiology, 62, 1958-1962. Gauthier, M. J., Lafay, B., Christen, R., Fernandez, L., Acquaviva, M., Bonin, P., Bertr, and J.-C. (1992). Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. International Journal of Systematic and Evolutionary Microbiology, 42, 568-576. Gradova, N. B., Gornova, I. B., Eddaudi, R., and Salina, R. N. (2003). Use of bacteria of the genus Azotobacter for bioremediation of oil-contaminated soils. Applied Biochemistry and Microbiology, 39, 279-281. Hill, S., and Postgate, J. R. (1969). Failure of putative nitrogen-fixing bacteria to Fix Nitrogen. Microbiology, 58, 277-285. Houlton, B. Z., Wang, Y.-P., Vitousek, P. M., and Field, C. B. (2008). A unifying framework for dinitrogen fixation in the terrestrial biosphere. Nature, 454, 327-330. Hwang, C. Y., and Cho, B. C. (2008). Cohaesibacter gelatinilyticus gen. nov., sp. nov., a marine bacterium that forms a distinct branch in the order Rhizobiales, and proposal of Cohaesibacteraceae fam. nov. International Journal of Systematic and Evolutionary Microbiology, 58, 267-277. Ivanova, E. P., Romanenko, L. A., Chun, J., Matte, M. H., Matte, G. R., Mikhailov, V. V., Svetashev, V. I., Huq, A., Maugel, T., and Colwell, R. R. (2000). Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov. International Journal of Systematic and Evolutionary Microbiology, 50, 901-907. Ivanova, E. P., Romanenko, L. A., Chun, J., Matte, M. H., Matte, G. R., Mikhailov, V. V., Svetashev, V. I., Huq, A., Maugel, T., and Colwell, R. R. (2000). Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov. International Journal of Systematic and Evolutionary Microbiology, 50, 901-907. Ivanova, E. P., Sawabe, T., Lysenko, A. M., Gorshkova, N. M., Hayashi, K., Zhukova, N. V., Nicolau, D. V., Christen, R., andMikhailov, V. V. (2002). Pseudoalteromonas translucida sp. nov. and Pseudoalteromonas paragorgicola sp. nov., and emended description of the genus. International Journal of Systematic and Evolutionary Microbiology, 52, 1759-1766. Jang, G. I., Hwang, C. Y., and Cho, B. C. (2011). Nitratireductor aquimarinus sp. nov., isolated from a culture of the diatom Skeletonema costatum, and emended description of the genus Nitratireductor. International Journal of Systematic and Evolutionary Microbiology, 61, 2676-2681. Kang, S. R., Srinivasan, S., and Lee, S. S. (2015). Vibrio oceanisediminis sp. nov., a nitrogen-fixing bacterium isolated from an artificial oil-spill marine sediment. Int J Syst Evol Microbiol, 65, 3552-3557. Kaye, J. Z., Márquez, M. C., Ventosa, A., and Baross, J. A. (2004). Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. International Journal of Systematic and Evolutionary Microbiology, 54, 499-511. Kim, I.-G., Lee, M.-H., Jung, S.-Y., Song, J. J., Oh, T.-K., and Yoon, J.-H. (2005). Exiguobacterium aestuarii sp. nov. and Exiguobacterium marinum sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. International Journal of Systematic and Evolutionary Microbiology, 55, 885-889. Kim, K. K., Jin, L., Yang, H. C., and Lee, S.-T. (2007). Halomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water. International Journal of Systematic and Evolutionary Microbiology, 57, 675-681. Kushmaro, A., Banin, E., Loya, Y., Stackebrandt, E., and Rosenberg, E. (2001). Vibrio shiloi sp. nov., the causative agent of bleaching of the coral Oculina patagonica. International Journal of Systematic and Evolutionary Microbiology, 51, 1383-1388. Labbé, N., Parent, S., and Villemur, R. (2004). Nitratireductor aquibiodomus gen. nov., sp. nov., a novel α-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada). International Journal of Systematic and Evolutionary Microbiology, 54, 269-273. LaRoche, J., and Breitbarth, E. (2005). Importance of the diazotrophs as a source of new nitrogen in the ocean. Journal of Sea Research, 53, 67-91. Lee, J. –C., Jeon, C. O., Lim, J. –M., Lee, S. –M., Lee, J. –M., Song, S. –M., Park, D.-J., Li, W.-J., and Kim, C. –J. (2005). Halomonas taeanensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 55, 2027–2032. Lee, J. V., Donovan, T. J., and Furniss, A. L. (1978). Characterization, Taxonomy, and Emended Description of Vibrio metschnikovii. International Journal of Systematic and Evolutionary Microbiology, 28, 99-111. Liu, Y., Shang, X., and Zeng, R. (2015). Permanent draft genome of acetaldehyde degradation bacterium, Shewanella sp. YQH10. Marine Genomics, 19, 9-11. Liu, Y., Shang, X.-X., Yi, Z.-W., Gu, L., and Zeng, R.-Y. (2015). Shewanella mangrovi sp. nov., an acetaldehyde-degrading bacterium isolated from mangrove sediment. International Journal of Systematic and Evolutionary Microbiology, 65, 2630-2634. Martin, J. P., and Haider, K. (1971). Microbial Activity In Relation To Soil Humus Formation. Soil Science, 111, 54-63. Matsuyama, H., Minami, H., Kasahara, H., Kato, Y., Murayama, M., and Yumoto, I. (2013). Pseudoalteromonas arabiensis sp. nov., a marine polysaccharide-producing bacterium. International Journal of Systematic and Evolutionary Microbiology, 63, 1805-1809. Matsuyama, H., Sawazaki, K., Minami, H., Kasahara, H., Horikawa, K., andYumoto, I. (2014). Pseudoalteromonas shioyasakiensis sp. nov., a marine polysaccharide-producing bacterium. International Journal of Systematic and Evolutionary Microbiology, 64, 101-106. Merlo, C., Reyna, L., Abril, A., Amé, M. V., and Genti-Raimondi, S. (2014). Environmental factors associated with heterotrophic nitrogen-fixing bacteria in water, sediment, and riparian soil of Suquía River. Limnologica - Ecology and Management of Inland Waters, 48, 71-79. Orth, R. J., Carruthers, T. J. B., Dennison, W. C., Duarte, C. M., Fourqurean, J. W., Heck, J. K. L., Hughes, A. R., Kendrick, G. A., Kenworthy, W. J., Olyarnik, S., Short, F. T., Waycott, M., and Williams, S. L. (2006). A global crisis for seagrass ecosystems. BioScience, 56, 987-996. Pajares, S., and Bohannan, B. J. M. (2016). Ecology of nitrogen fixing, nitrifying, and denitrifying microorganisms in tropical forest soils. Frontiers in Microbiology, 7, 1045. Park, S., Park, J.-M., Jung, Y.-T., Seong, C. N., andYoon, J.-H. (2014). Mesoflavibacter sabulilitoris sp. nov., isolated from seashore sand. International Journal of Systematic and Evolutionary Microbiology, 64, 3743-3748. Patriquin, D., and Knowles, R. (1972). Nitrogen fixation in the rhizosphere of marine angiosperms. Marine Biology, 16, 49-58. Pollard, P.C., and D. J. W. Moriarty. (1991). Organic-carbon decomposition, primary and bacterial productivity, and sul-phate reduction in tropical seagrass beds of the Gulf of Car-pentaria, Australia. Marine Ecology Progress Series, 69:149–159. Qu, L., Lai, Q., Zhu, F., Hong, X., Sun, X., and Shao, Z. (2011). Cohaesibacter marisflavi sp. nov., isolated from sediment of a seawater pond used for sea cucumber culture, and emended description of the genus Cohaesibacter. International Journal of Systematic and Evolutionary Microbiology, 61, 762-766. Quillaguaman, J., Hashim, S., Bento, F., Mattiasson, B., and Hatti‐Kaul, R. (2005). Poly (β‐hydroxybutyrate) production by a moderate halophile, Halomonas boliviensis LC1 using starch hydrolysate as substrate. J Appl Microbiol 99, 151–157. Rameshkumar, N., Fukui, Y., Sawabe, T., and Nair, S. (2008). Vibrio porteresiae sp. nov., a diazotrophic bacterium isolated from a mangrove-associated wild rice (Porteresia coarctata Tateoka). International Journal of Systematic and Evolutionary Microbiology, 58, 1608-1615. Rameshkumar, N., Sproer, C., Lang, E., and Nair, S. (2010). Vibrio mangrovi sp. nov., a diazotrophic bacterium isolated from mangrove-associated wild rice (Poteresia coarctata Tateoka). FEMS Microbiology Letters, 307, 35-40. Reller, L. B., Weinstein, M. P., and Petti, C. A. (2007). Detection and identification of microorganisms by gene amplification and sequencing. Clinical Infectious Diseases 44, 1108–1114. Reimer, J. D., Santos, M. E. A., Kise, H., Neo, M. L., Chen, C. A., and Soong, K. (2017). Diversity of Zoantharia (Anthozoa: Hexacorallia) at Dongsha Atoll in the South China Sea. Regional Studies in Marine Science, 12, 49-57. Ribes, M., Dziallas, C., Coma, R., and Riemann, L. (2015). Microbial Diversity and Putative Diazotrophy in High- and Low-Microbial-Abundance Mediterranean Sponges. Appl Environ Microbiol, 81, 5683-5693. Romanenko, L. A., Tanaka, N., Svetashev, V. I., and Falsen, E. (2013). Description of Cobetia amphilecti sp. nov., Cobetia litoralis sp. nov. and Cobetia pacifica sp. nov., classification of Halomonas halodurans as a later heterotypic synonym of Cobetia marina and emended descriptions of the genus Cobetia and Cobetia marina. International Journal of Systematic and Evolutionary Microbiology, 63, 288-297. Romanenko, L. A., Zhukova, N. V., Rohde, M., Lysenko, A. M., Mikhailov, V. V., and Stackebrandt, E. (2003). Pseudoalteromonas agarivorans sp. nov., a novel marine agarolytic bacterium. International Journal of Systematic and Evolutionary Microbiology, 53, 125-131. Romano, I., Lama, L., Nicolaus, B., Poli, A., Gambacorta, A., and Giordano, A. (2006). Halomonas alkaliphila sp. nov., a novel halotolerant alkaliphilic bacterium isolated from a salt pool in Campania (Italy). The Journal of General and Applied Microbiology, 52, 339-348. Rossi-Tamisier, M., Benamar, S., Raoult, D., and Fournier, P.-E. (2015). Cautionary tale of using 16S rRNA gene sequence similarity values in identification of human-associated bacterial species. International Journal of Systematic and Evolutionary Microbiology, 65, 1929-1934. Scavotto, R. E., Dziallas, C., Bentzon-Tilia, M., Riemann, L., and Moisander, P. H. (2015). Nitrogen-fixing bacteria associated with copepods in coastal waters of the North Atlantic Ocean. Environmental Microbiology, 17, 3754-3765. Schiewe, M. H., Trust, T. J., andCrosa, J. H. (1981). Vibrio ordalii sp. nov.: A causative agent of vibriosis in fish. Current Microbiology, 6, 343-348. Shieh, W. Y., Chen, Y. –W., Chaw, S. –M., and Chiu, H. –H. (2003). Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53, 479–484. Shieh, W. Y., Simidu, U. and Maruyama, Y. (1989). Enumeration and characterization of nitrogen-fixing bacteria in an eelgrass (Zostera marina) bed. Microbial Ecology, 18, 249-259. Shiozaki, T., Ijichi, M., Kodama, T., Takeda, S., and Furuya, K. (2014). Heterotrophic bacteria as major nitrogen fixers in the euphotic zone of the Indian Ocean. Global Biogeochemical Cycles, 28, 1096-1110. Short, F., Carruthers, T., Dennison, W., and Waycott, M. (2007). Global seagrass distribution and diversity: A bioregional model. Journal of Experimental Marine Biology and Ecology, 350, 3-20. Skovhus, T. L., Holmström, C., Kjelleberg, S., and Dahllöf, I. (2007). Molecular investigation of the distribution, abundance and diversity of the genus Pseudoalteromonas in marine samples. FEMS Microbiology Ecology, 61, 348-361. Sugahara, I., Kimura, T., Aizawa, M., and Hayakawa, Y. (1989). Generic composition and physiological properties of nitrogen-scavenging bacteria isolated from marine environments. Nippon Suisan Gakkaishi, 55, 1441-1447. Sun, F., Zhang, X., Zhang, Q., Liu, F., Zhang, J., and Gong, J. (2015). Seagrass (Zostera marina) Colonization Promotes the Accumulation of Diazotrophic Bacteria and Alters the Relative Abundances of Specific Bacterial Lineages Involved in Benthic Carbon and Sulfur Cycling. Appl Environ Microbiol, 81, 6901-6914. Sundstr, K. R., xf, and Huss, K. (1975). Effect of nitrogen-fixing bacteria on mineralization in raw humus. Oikos, 26, 147-151. Teske, A., Cypionka, H., Holt, J. G., & Krieg, N. R. (2007). Enrichment and isolation methods for general and molecular microbiology, third edition (pp. 215–269): American Society of Microbiology. Thompson, F. L., Li, Y., Gomez-Gil, B., Thompson, C. C., Hoste, B., Vandemeulebroecke, K., Rupp, G. S., Pereira, A., De Bem, M. M., Sorgeloos, P., and Swings, J. (2003). Vibrio neptunius sp. nov., Vibrio brasiliensis sp. nov. and Vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps). International Journal of Systematic and Evolutionary Microbiology, 53, 245-252. Thompson, F. L., Thompson, C. C., Hoste, B., Vandemeulebroecke, K., Gullian, M., andSwings, J. (2003). Vibrio fortis sp. nov. and Vibrio hepatarius sp. nov., isolated from aquatic animals and the marine environment. International Journal of Systematic and Evolutionary Microbiology, 53, 1495-1501. Turk-Kubo, K. A., Karamchandani, M., Capone, D. G., and Zehr, J. P. (2014). The paradox of marine heterotrophic nitrogen fixation: abundances of heterotrophic diazotrophs do not account for nitrogen fixation rates in the Eastern Tropical South Pacific. Environ Microbiol, 16, 3095-3114. Ueda, T., Suga, Y., Yahiro, N., and Matsuguchi, T. (1995). Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences. Journal of Bacteriology, 177, 1414-1417. Urbanczyk, H., Ast, J. C., Higgins, M. J., Carson, J., and Dunlap, P. V. (2007). Reclassification of Vibrio fischeri, Vibrio logei, Vibrio salmonicida and Vibrio wodanis as Aliivibrio fischeri gen. nov., comb. nov., Aliivibrio logei comb. nov., Aliivibrio salmonicida comb. nov. and Aliivibrio wodanis comb. nov. International Journal of Systematic and Evolutionary Microbiology, 57, 2823-2829. Voss, M., Bange, H. W., Dippner, J. W., Middelburg, J. J., Montoya, J. P., and Ward, B. (2013). The marine nitrogen cycle: recent discoveries, uncertainties and the potential relevance of climate change. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, 368, 20130121. Wang, Y., Zhang, X.-H., Yu, M., Wang, H., and Austin, B. (2010). Vibrio atypicus sp. nov., isolated from the digestive tract of the Chinese prawn. International Journal of Systematic and Evolutionary Microbiology, 60, 2517-2523. Welsh, D. T., Bourgués, S., de Wit, R., and Herbert, R. A. (1996). Seasonal variations in nitrogen-fixation (acetylene reduction) and sulphate-reduction rates in the rhizosphere of Zostera noltii: nitrogen fixation by sulphate-reducing bacteria. Marine Biology, 125, 619-628. Yoon, J.-H., Lee, M.-H., Oh, T.-K., andPark, Y.-H. (2005). Muricauda flavescens sp. nov. and Muricauda aquimarina sp. nov., isolated from a salt lake near Hwajinpo Beach of the East Sea in Korea, and emended description of the genus Muricauda. International Journal of Systematic and Evolutionary Microbiology, 55, 1015-1019. Zehr, J. P., Jenkins, B. D., Short, S. M., and Steward, G. F. (2003). Nitrogenase gene diversity and microbial community structure: a cross-system comparison. Environmental Microbiology, 5, 539-554. Zehr, J. P., Mellon, M. T., and Zani, S. (1998). New Nitrogen-Fixing Microorganisms Detected in Oligotrophic Oceans by Amplification of Nitrogenase (nifH) Genes. Appl Environ Microbiol, 64, 3444-3450. Zhang, Y., Dong, J., Yang, Z., Zhang, S., and Wang, Y. (2008). Phylogenetic diversity of nitrogen-fixing bacteria in mangrove sediments assessed by PCR–denaturing gradient gel electrophoresis. Archives of Microbiology, 190, 19. Zolg, W., and Ottow, J. C. G. (1975). Pseudomonas glathei sp. nov., a new nitrogen-scavenging rod isolated from acid lateritic relicts in Germany. Zeitschrift für allgemeine Mikrobiologie, 15, 287-299. 林幸助、謝莉顒、劉弼仁 (2004)。東沙島海草種類與豐度,兼記四種台灣新紀錄種。國立中興大學生命科學系 孫筱雲、柯佳吟、李培芬 (2012)。東沙島周圍海草分布特徵。航測及遙測學刊,第十六卷,第3期,第167-180頁。 冼宜樂、鐘金水、林金榮 (2014)。談漂流海草族群擴散機制。水產試驗所澎湖海洋生物研究中心。水試專訊。第45期,第24-28頁。 林勇民(1993)。瘤砂葵(Palyhoa tuberculosa Esper)及一種軟海綿(Halichondria sp.)上異營性細菌固氮之研究。國立臺灣大學海洋研究所碩士論文。台北。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/67375 | - |
dc.description.abstract | 固氮作用是氮循環中一個重要環節,自然界可利用氮源大多經由具有固氮能力細菌行生化作用,固氮細菌的種類多元,並普遍存在海洋各種環境中。
本研究針對生長在台灣西南海域中東沙島小潟湖的海草床以及非海草床沉積土中固氮細菌以及氮源清除細菌進行了分離和分類探討。 使用NFG (nitrogen-free glucose) 液體培養基在厭氧條件下增菌培養,配合並利用最大可能計數法 (most-probable-number counts) ,估得潟湖沉積物中固氮細菌/氮源清除菌在不同海草床沉積物的菌數介於 2.8×〖10〗^3至 4.6×〖10〗^5 cells/g wet wt.,在非海草床沉積物的菌數介於 1.0×〖10〗^1至 7.0×〖10〗^3 cells/g wet wt.。 從培養液明顯變混濁試管共分離出64株固氮/氮源清除細菌,進行其各種生理生化特性檢測後發現,分離株大部分為革蘭氏陰性菌,只有一株為革蘭氏陽性。利用16S rRNA 基因列比對進行親源分析,可將64株分離株分為17個屬層級菌群,其中Vibrio為最大組成菌屬。利用固氮酶(nitrogenase)基因(nifH) 中保守區域引子對,透過聚合酶反應 (polymerase chain reaction) 分析固氮酶基因序列多樣性,從歸類為 Vibrio、Shewanella、Nesiotobacter以及Nitratireductor等屬之 15 株細菌檢測出nifH基因,其中以Vibrio占最多 (12株)。依照16S rRNA 基因序列比對結果,共有5株含有固氮基因分離株代表株與最相近標準株序列相似度低於區分種間閾值(98.7%),為潛在新種固氮菌,其中代表株A3以及 B3-1 與最相近標準株序列相似度低於可區分細菌屬間的閾值(95.0 %),再比對相關生理、生化特性後,初步研判具有發表為新屬潛力。 | zh_TW |
dc.description.abstract | Nitrogen fixation is an essential step in nitrogen cycle since atmospheric N2 is inaccessible to most organisms, therefore it is the main process that N2 gas is transformed into ammonia. N2 fixation is exclusively carried out by specialized bacteria which are called diazotrophs. Diazotrophs exist in a diverse marine environment. The aim of the study was to isolate and characterize the nitrogen-fixing/nitrogen-scavenging bacteria from a lagoon in Dongsha Island. The abundance of the nitrogen-fixing/nitrogen-scavenging bacteria in seagrass-bed and non-seagrass-bed sediments were enumerated by the most-probable-number (MPN) method and incubated in NFG (nitrogen-free glucose) media under anaerobic condition. The values of MPN ranged between 2.8×〖10〗^3and 4.6×〖10〗^5 cells/g wet wt. in seagrass-bed sediments and between 1.0×〖10〗^1 and 7.0×〖10〗^3 in non-seagrass-bed sediments.
Sixty-four nitrogen-fixing/nitrogen-scavenging bacteria isolates were isolated. Based on their physiological and biochemical characteristics, most isolates were Gram negative, only one isolate was Gram positive. According to phylogenetic analysis of the 16S rRNA gene, all isolates were divided into 17 groups based on genus level. Majority of them were the genus Vibrio consisted the majority. The primer pairs in conserved regions of nitrogenase gene (nifH) were used to determine the nitrogen-fixing isolates with PCR. Fifteen isolates belonging to Vibrio, Shewanella, Nitratireductor and Nesiotobacter genus had the nifH gene, and most of them were from the genus of Vibrio. Five strains containing amplified nifH gene shared low similarity of 16S rRNA sequences with the known species, which might be classified as novel nitrogen-fixing species. Among them, strain A3 and B3-1 might be proposed two species in a novel genus. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T01:29:45Z (GMT). No. of bitstreams: 1 ntu-106-R04241206-1.pdf: 3007272 bytes, checksum: dddd064e582ade86b61e714a2f95bd77 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 中文摘要 ………………………………………………………………IV
Abstract ………………………………………………………………..V 第一章 前言 1 第一節 氮與環境 1 第二節 生物性固氮作用 1 第三節 海草床與生物性固氮 2 第四節 固氮酶與固氮基因 3 第五節 固氮細菌以及氮清除細菌 4 第六節 研究動機與目的 5 第二章 材料與方法 7 第一節 樣本地點 7 第二節 培養基 9 第三節 計數、培養與分離 9 第四節 分離株之形態及生理、生化特性檢測 10 第五節 16S rRNA基因序列之分析 12 第六節 固氮基因(nifH)之分析 14 第七節 新種細菌生化特性檢測 15 第三章 結果 17 第一節 MPN計數法 17 第二節 短片段16S rRNA 序列分析及分群 17 第三節 生理、生化特性測試並挑選代表株 17 第四節 分群代表株16S rRNA 基因序列分析 18 第五節 固氮基因分析 22 第六節 新種細菌代表株A3以及代表株B3-1 23 第四章 討論 27 第一節 東沙島潟湖之固氮菌/氮清除菌菌數量 27 第二節 分群代表株之生理生化特性以及分群 27 第三節 固氮/氮清除分離株親緣關係分析之探討 38 第四節 含有固氮基因分離株分析之探討 39 第五節 新種細菌A3與B3-1 之探討 40 第五章 結論 42 參考文獻 ………………………………………………………………43 表………………………………………………………………..54 圖………………………………………………………………..66 附表……………………………………………………………..74 | |
dc.language.iso | zh-TW | |
dc.title | 東沙島潟湖沉積物中固氮細菌及氮清除細菌的分離和特性研究 | zh_TW |
dc.title | Isolation and characterization of nitrogen-fixing/nitrogen-scavenging bacteria from a lagoon in Dongsha Island | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李重義,陳卓昇,林育德,簡文達 | |
dc.subject.keyword | 沉積物,平板計數法,東沙,固氮菌,16S rRNA基因, | zh_TW |
dc.subject.keyword | Dongsha Island,lagoon,seagrass-bed sediment,nitrogen-fixing bacteria,nitrogen-scavenging bacteria,16S rRNA gene, | en |
dc.relation.page | 79 | |
dc.identifier.doi | 10.6342/NTU201702499 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-08-04 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 海洋研究所 | zh_TW |
顯示於系所單位: | 海洋研究所 |
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