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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/66353完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李百祺 | |
| dc.contributor.author | Yi-Han Li | en |
| dc.contributor.author | 李浥涵 | zh_TW |
| dc.date.accessioned | 2021-06-17T00:31:37Z | - |
| dc.date.available | 2014-03-19 | |
| dc.date.copyright | 2012-03-19 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-02-10 | |
| dc.identifier.citation | [1] Michael LH, Entman ML, Hartley CJ, Youker KA, Zhu J, Hall SR, Hawkins HK, Berens K, Ballantyne CM, Myocardial ischemia and reperfusion: a murine model, Am J Physiol; 269(6 Pt 2):H2147–54 Dec 1995.
[2] Tarnavski O, McMullen JR, Schinke M, Nie Q, Kong S, Izumo S, Mouse cardiac surgery: comprehensive techniques for the generation of mouse models of human diseases and their application for genomic studies, Physiol Genomics; 13;16:(3)349–360 Feb 2004. [3] Scherrer-Crosbie M, Thibault HB, Echocardiography in Translational Research: of Mice and Men, J Am Soc Echocardiogr; 21(10):1083–92 Oct 2008. [4] 何祚明, 高頻超音波影像系統, 國立台灣大學電機工程學研究所碩士論文, June 2002. [5] Respress JL, Wehrens XH, Transthoracic Echocardiography in Mice, J Vis Exp. 28; (39), pii: 1738 May 2010. [6] Spurney CF, Andrew Hoey, Yves Fromes,Paul Herijgers, Non-invasive echocardiographic assessment of cardiac function in the mdx mouse model, Aug 2008. [7] Thomas G. Nyland, John S. Mattoon, Small Animal Diagnostic Ultrasound, W.B. Saunders Company, 1995. [8] Reddy AK, Amador-Noguez D, Darlington GJ, Scholz BA, Michael LH, Hartley CJ, Entman ML, and Taffet GE, Cardiac Function in Young and Old Little Mice, J Gerontol A Biol Sci; 62(12): 1319–1325 Dec 2007. [9] Steven J Lavine, Donald A Conetta, Comparison of the effect of pressure loading on left ventricular size, systolic and diastolic function in canines with left ventricular dysfunction with preserved and reduced ejection fraction, Cardiovasc Ultrasound; 6:57 2008. [10] Emmanoiil NK, Anna T. G, John EP, Athanassios VK, Nearchos S N, Myocardial Performance Index (Tei Index): Evaluating its Application to Myocardial Infarction, Hellenic J Cardiol; 50: 60-65 2009. [11] 廖朝崧等人, 心臟科超音波學, 藝軒出版社, October 2006. [12] Teicholz LE, Kreulen T, Herman M V, Gorlin R, Problems in echocardiographic volume determinations: echocardiographic-angiographic correlations in the presence or absence of asynergy, Am J Cardiol; 37:7 1976. [13] 李承翰, 高頻超音波血流成像, 國立台灣大學電機工程學研究所碩士論文, June 2006. [14] Reichek N, Devereux RB, Reliable estimation of peak left venticular systolic pressure by M-mode echocardiographic determined end-diastolic relative wall thickness: identification of severe valvular aortic stenosis in adult patients, Am Heart J; 103:202 1982. [15] Cittadini A, Mantzoros CS, Hampton TG, Travers KE, Katz SE, Morgan JP, Cardiovascular abnormalities in transgenic mice with reduced brown fat: an animal model of human obesity, Circulation; 100:2177–2183 1999. [16] Schultz JE, Witt SA, Nieman ML, Reiser PJ, Engle SJ, Zhou M, Fibroblast growth factor-2 mediates pressure-induced hypertrophic response, J Clin Invest; 104:709–719 1999. [17] Tanaka N, Dalton N, Mao L, Rockman HA, Peterson KL, Gottshall KR, Transthoracic echocardiography in models of cardiac disease in the mouse, Circulation; 94:1109–1117 1996. [18] Manning WJ, Wei JY, Katz SE, Litwin SE, Douglas PS, In vivo assessment of LV mass in mice using high-frequency cardiac ultrasound: necropsy validation, Am J Physiol; 266:H1672–H1675 1994. [19] Yang XP, Liu YH, Rhaleb NE, Kurihara N, Kim HE, Carretero OA, Echocardiographic assessment of cardiac function in conscious and anesthetized mice, Am J Physiol; 277:H1967–H1974 1999. [20] Gao E, Lei YH, Shang X, Huang ZM, Zuo L, Boucher M, Fan Q, Chuprun JK, Ma XL, and Koch WJ, A Novel and Efficient Model of Coronary Artery Ligation and Myocardial Infarction in the Mouse, Circ Res.; 107:00-00 2010. [21] 王聖維, 超音波對比劑應用於血流參數計算之研究, 國立台灣大學電機工程學研究所碩士論文, June 2001. [22] Liang HD, K Blomley MJ, The role of ultrasound in molecular imaging, British Journal of Radiology; 76:S140–S150 2003. [23] Mary E. Loveless, et al., A Method for Assessing the Microvasculature in a Murine Tumor Model Using Contrast-Enhanced Ultrasonography, J Ultrasound Med.; 27(12): 1699–1709 Dec 2008. [24] Scherrer-Crosbie M, Steudel W, Ullrich R, Hunziker PR, Liel-Cohen N, Newell J, et al., Echocardiographic determination of risk a size in a murine model of myocardial ischemia, Am J Physiol; 277:H986–H992 1999. [25] Lafitte S, Masugata H, Peters B, Togni M, Strachan M, Yao B, et al., Accuracy and reproducibility of coronary flow rate assessment by real-time contrast echocardiography: in vitro and in vivo studies, J Am Sociality Echocardiography;14:1010–1019 2001. [26] Raher MJ, Thibault H, Poh KK, Liu R, Halpern EF, Derumeaux G, et al., In Vivo Characterization of Murine Myocardial Perfusion Using Myocardial Contrast Echocardiography, Validation and Application in Nitric Oxide Synthase 3-Deficient Mice. Circulation; 116:1250–1257 2007. [27] Wei K, Jayaweera AR, Firoozan S, Linka A, Skyba DM, Kaul S, Quantification of myocardial blood flow with ultrasound-induced destruction of microbubbles administered as a constant venous infusion, Circulation; 97:473–483 1998. [28] Wei K, Ragosta M, Thorpe J, Coggins M, Moos S, Kaul S, Noninvasive quantification of coronary blood flow reserve in humans using myocardial contrast echocardiography, Circulation; 103:2560–2565 2001. [29] Phillips, PJ, Contrast pulse sequences (CPS): imaging nonlinear microbubbles. Paper presented at: Ultrasonics Symposium, IEEE. Atlanta, GA, USA: 2001. [30] French BA, Li Y, Klibanov AL, Yang Z, Hossack JA, 3D perfusion mapping in post-infarct mice using myocardial contrast echocardiography, Ultrasound Med Biol; 32:805–815 2006. [31] 陳業鵬, 謝禮全, 林楨智, 羅秉漢, 張坤正, 洪瑞松, 心電圖與心臟超音波診斷左心室肥厚的相關性, 內科學誌; 20：99-105 2009. [32] Wikstrom J, Gronros J, Bergstrom G, Gan LM, Functional and morphologic imaging of coronary atherosclerosis in living mice using high-resolution color Doppler echocardiography and ultrasound biomicroscopy, J Am Coll Cardiol;46:720–727 2005. [33] Gronros J, Wikstrom J, Hagg U, Wandt B, Gan LM, Proximal to middle left coronary artery flow velocity ratio, as assessed using color Doppler echocardiography, predicts coronary artery atherosclerosis in mice, Arterioscler Thromb Vasc Biol; 26:1126–1131 2006. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/66353 | - |
| dc.description.abstract | 二十世紀中葉開始，動物模型廣泛被用於了解人類疾病的生理及病理情形，每年有7500萬的囓齒動物被應用於微生物、藥理、毒物和免疫學等相關研究，其中小鼠占最多數。使用非侵入性的影像系統有助於長時間觀察小鼠疾病模型的變化，以獲得更完整訊息及減少小鼠犧牲數量。
超音波影像系統可適用於不同部位之檢測，例如腹部、心臟、神經血管及婦產科超音波等。因應小鼠模型所發展的高頻超音波亦具相同優勢外，可及時及測量血流訊息，更適用於評估心臟的功能表現。其中灰階超音波、運動模式、杜卜勒模式及對比劑模式可分別提供不同影像訊息，為了驗證本實驗室所研發的高頻超音波系統正確性與穩定性，綜合上述模式的臨床評估方法，用於已知的小鼠疾病模型，並且以區別性與再現性結果，證明其系統正確性、穩定性及方法的適用性。 從實驗結果發現，心肌肥厚小鼠模型與對照組小鼠在28週觀察過程中，Hypertrophiy Index皆有顯著差異性(p-value<0.04)。而心肌梗塞模型與對照組小鼠在術前與術後兩週及四週的Tei Index 相較下，皆有顯著差異性(p-value<0.005)，進一步與病理切片結果驗證，超音波影像術後的心肌厚度為0.43±0.13mm與病理切片的結果0.5mm是接近的。此外在對比劑實驗中，結紮手術後的小鼠心臟灌注實驗時其前壁與側壁分別增強約5.64-8.88dB及 6.52-10.46dB，其中前壁梗塞處略低於側壁正常的區域的平均顯影強度，再者平均穿流時間及達最大峰值時間相較於正常區域則較為延長。 綜合上述結果，本實驗室中所建立的40 MHz高頻超音波影像系統，可運用心臟相關的不同臨床方法有效區別正常與異常小鼠模型，未來將持續研發其他相關應用如三維超音波及組織杜卜勒等，以期未來對臨床前的生物醫學研究有更多之貢獻。 | zh_TW |
| dc.description.abstract | Mouse is the most common animal model used to understand human disease from the mid-twentieth century. High frequency ultrasound system (HFUS), with the advantages of real-time imaging and non-invasive properties, is suitable to trace the progression of disease without sacrificing animals.
In this thesis, we studied the accuracy and stability of the HFUS with cardiomyopathy hypertrophy and myocardial infarction models of mice. First, the cardiomyopathy hypertrophy mice have significant difference with control mice in Hypertrophy Index during 4 months. Second, the myocardial infarction model of post-surgery two weeks and four weeks also has significant difference with control mice in Tei Index. About the myocardial thickness, the biopsy result (0.5mm) is closed to the estimation of HFUS(0.43 ± 0.13mm). In contrast perfusion experiments, there is slightly lower intensity in anterior infarct region (5.64-8.88dB) than normal lateral region(6.52-10.46dB). Moreover, the Mean Transit Time and Peak Time are also longer than normal region. Our study demonstrated the use of HFU system in pre-clinical study and we plan to provide more valuable information with 3D ultrasound and tissue Doppler in the future. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T00:31:37Z (GMT). No. of bitstreams: 1 ntu-101-P98945001-1.pdf: 4142603 bytes, checksum: 8a534ccbcffe6414680b2d1a9418756c (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 目錄
致謝----------------------------------------------------------------------------------Ⅱ 中文摘要----------------------------------------------------------------------------Ⅲ 英文摘要----------------------------------------------------------------------------Ⅳ 目錄----------------------------------------------------------------------------------Ⅴ 圖目錄-------------------------------------------------------------------------------Ⅶ 表目錄-------------------------------------------------------------------------------Ⅹ 第一章 緒論 1.1 前言--------------------------------------------------------------------------------------1 1.2 高頻超音波的簡介--------------------------------------------------------------------2 1.3 高頻超音波的系統及原理-----------------------------------------------------------2 1.4 高頻超音波的應用--------------------------------------------------------------------6 1.5 研究動機與目標-----------------------------------------------------------------------9 第二章 高頻超音波中不同模式的心臟學應用 2.1 灰階模式---------------------------------------------------------------------------------13 2.2 運動模式---------------------------------------------------------------------------------14 2.3 彩色杜普勒模式------------------------------------------------------------------------15 2.4 脈衝波杜普勒模式---------------------------------------------------------------------16 2.5 對比劑模式------------------------------------------------------------------------------17 第三章 小鼠心臟實驗 3.1 心肌肥厚症鼠的心臟超音波實驗---------------------------------------------------21 3.1.1 心臟實驗的取像方式----------------------------------------------------------22 3.1.2 心肌肥厚症的致病機轉-------------------------------------------------------24 3.1.3 心臟超音波實驗----------------------------------------------------------------24 3.1.4 突變鼠心臟功能的追蹤-------------------------------------------------------25 3.1.5 實驗結果分析-------------------------------------------------------------------30 3.2 冠狀動脈結紮手術鼠的心臟超音波實驗 3.2.1 冠狀動脈致病機轉-------------------------------------------------------------35 3.2.2 結紮手術的流程----------------------------------------------------------------37 3.2.3 冠狀動脈結紮手術鼠心臟功能的追蹤-------------------------------------38 3.2.4 實驗結果分析-------------------------------------------------------------------42 3.2.5 冠狀動脈結紮手術鼠術前與術後之心臟功能的變化-------------------44 3.2.6 冠狀動脈結紮手術鼠之心臟病理切片結果-------------------------------49 第四章 對比劑在高頻超音波的應用 4.1 對比劑的簡介---------------------------------------------------------------------------53 4.2 對比劑的應用---------------------------------------------------------------------------53 4.3 仿體實驗---------------------------------------------------------------------------------54 4.3.1血液仿體實驗下高頻超音波最理想的掃描參數---------------------------54 4.3.2Targeson體外實驗下高頻超音波最理想的掃描參數----------------------56 4.4 小鼠心臟灌注之超音波實驗---------------------------------------------------------60 4.4.1 Sonovue MBS的粒徑分析------------------------------------------------------61 4.4.2正常鼠心臟灌注情形------------------------------------------------------------62 4.4.3冠狀動脈結紮手術鼠心臟灌注情形------------------------------------------66 4.5 腫瘤鼠皮下腫瘤之超音波實驗------------------------------------------------------70 4.5.1腫瘤鼠皮下腫瘤的血管新生情形---------------------------------------------70 4.5.2 Targeson MBS的粒徑分析-----------------------------------------------------73 第五章 結論與未來工作-----------------------------------------------------------------------77 第六章 參考文獻--------------------------------------------------------------------------------81 | |
| dc.language.iso | zh-TW | |
| dc.subject | 小動物模型 | zh_TW |
| dc.subject | 高頻超音波 | zh_TW |
| dc.subject | 心肌肥厚症 | zh_TW |
| dc.subject | 心肌梗塞症 | zh_TW |
| dc.subject | Cardiomyopathy Hypertrophy | en |
| dc.subject | High-Frequency Ultrasound | en |
| dc.subject | Myocardial Infarction Disease | en |
| dc.subject | Small Animal Models | en |
| dc.title | 臨床前超音波影像之心臟功能研究應用 | zh_TW |
| dc.title | Applications of Pre-Clinical Ultrasound in Cardiac Research | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林隆君,沈哲州,鄭耿璽,廖愛禾 | |
| dc.subject.keyword | 高頻超音波,小動物模型,心肌肥厚症,心肌梗塞症, | zh_TW |
| dc.subject.keyword | High-Frequency Ultrasound,Small Animal Models,Cardiomyopathy Hypertrophy,Myocardial Infarction Disease, | en |
| dc.relation.page | 84 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-02-10 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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