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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 徐善慧(Shan-hui Hsu) | |
dc.contributor.author | Ting-Yu Lin | en |
dc.contributor.author | 林庭宇 | zh_TW |
dc.date.accessioned | 2021-06-17T00:30:09Z | - |
dc.date.available | 2015-03-19 | |
dc.date.copyright | 2012-03-19 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-02-13 | |
dc.identifier.citation | [1] Michael HR, Edward JR, Lynn JR. 林志聰等編譯,組織學,藝軒出版社, 民82 ;
123-138. [2] Getgood A, Brooks R, Fortier L, Rushton N. Articular cartilage tissue engineering: today’s research, tomorrow’s practice? J Bone Joint Surg Br 2009,91:565-76. [3] Wang L, Detamore MS. Tissue engineering the mandibular condyle. Tissue Eng 2007,13:1955-71. [4]Mehlhorn AT, Zwingmann J, Finkenzeller G, Niemeyer P, Dauner M, Stark B, et al. Chondrogenesis of adipose-derived adult stem cells in a poly-lactide-co-glycolide scaffold. Tissue Eng Part A 2009,15:1159-1167. [5] Hsu SH, Yen HJ, Tseng CS, Cheng CS, Tsai CL. Evaluation of the growth of chondrocytes and osteoblasts seeded into precision scaffolds fabricated by fused deposition manufacturing. J Biomed Mater Re Part B-Appl Biomate.2007,80B:519-527. [6] Park SN, Park JC, Kim HO, Song MJ, Suh H. Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking. Biomaterials 2002,23:1205-1212. [7] Pfeiffer E, Vickers SM, Frank E, Grodzinsky AJ, Spector M. The effects of glycosaminoglycan content on the compressive modulus of cartilage engineered in type II collagen scaffolds. Osteoarthr Cartil 2008,16:1237-1244. [8] Harley BAC, Gibson LJ. In vivo and in vitro applications of collagen-GAG scaffolds. Chemical Engineering Journal 2008,137:102-121. [9] Choi KH, Choi BH, Park SR, Kim BJ, Min BH. The chondrogenic differentiation of mesenchymal stem cells on an extracellular matrix scaffold derived from porcine chondrocytes. Biomaterials 2010,31:5355-5365. [10] Pfeiffer E, Vickers SM, Frank E, Grodzinsky AJ, Spector M. The effects of glycosaminoglycan content on the compressive modulus of cartilage engineered in type II collagen scaffolds. Osteoarthr. Cartil. 2008,16:1237-1244. [11] Hsu SH, Tsai CL, Tang CM. Evaluation of cellular affinity and compatibility to biodegradable polyesters and type-II collagen-modified scaffolds using immortalized rat chondrocytes. Artificial Organs 2002,26:647-658. [12] Lin CH, Su JM, Hsu SH. Evaluation of type II collagen scaffolds reinforced by poly(epsilon-caprolactone) as tissue-engineered trachea. Tissue Engineering Part C-Methods 2008,14:69-77. [13] Yen HJ, Hsu SH, Tseng CS, Huang JP, Tsai CL. Fabrication of precision scaffolds using liquid-frozen deposition manufacturing for cartilage tissue engineering. Tissue Eng Part A 2009,15:965-975. [14] Tan HP, Wu JD, Lao LH, Gao CY. Gelatin/chitosan/hyaluronan scaffold integrated with PLGA microspheres for cartilage tissue engineering. Acta Biomaterialia 2009,5:328-337. [15] Kim BS, Park IK, Hoshiba T, Jiang HL, Choi YJ, Akaike T, et al. Design of artificial extracellular matrices for tissue engineering. Prog Polym Sci 2011,36:238-268. [16] Lu Z, Doulabi BZ, Huang C, Bank RA, Helder MN. Collagen type ii enhances chondrogenesis in adipose tissue-derived stem cells by affecting cell shape. Tissue Eng Part A 2010,16:81-90. [17] Pulkkinen HJ, Tiitu V, Valonen P, Jurvelin JS, Lammi MJ, Kiviranta I. Engineering of cartilage in recombinant human type II collagen gel in nude mouse model in vivo. Osteoarthr Cartil 2010,18:1077-1087. [18] Ragetly G, Griffon DJ, Chung YS. The effect of type II collagen coating of chitosan fibrous scaffolds on mesenchymal stem cell adhesion and chondrogenesis. Acta Biomaterialia 2010,6:3988-3997. [19] Ko CS, Huang JP, Huang CW, Chu IM. Type II collagen-chondroitin sulfate-hyaluronan scaffold cross-linked by genipin for cartilage tissue engineering. J Biosci Bioeng 2009,107:177-182. [20] Wu SC, Chang JK, Wang CK, Wang GJ, Ho ML. Enhancement of chondrogenesis of human adipose derived stem cells in a hyaluronan-enriched microenvironment. Biomaterials 2010,31:631-640. [21] Kayakabe M, Tsutsumi S, Watanabe H, Kato Y, Takagishi K. Transplantation of autologous rabbit BM-derived mesenchymal stromal cells embedded in hyaluronic acid gel sponge into osteochondral defects of the knee. Cytotherapy 2006;8:343–53. [22] Toh WS, Lee EH, Guo XM, Chan JKY, Yeow CH, Choo AB, et al. Cartilage repair using hyaluronan hydrogel-encapsulated human embryonic stem cell-derived chondrogenic cells. Biomaterials 2010,31:6968-6980. [23] Barbucci R, Lamponi S, Borzacchiello A, Ambrosio L, Fini M, Torricelli P, et al. Hyaluronic acid hydrogel in the treatment of osteoarthritis. Biomaterials 2002,23:4503-4513. [24] Na K, Kim S, Woo DG, Sun BK, Yang HN, Chung HM, et al. Synergistic effect of TGFbeta-3 on chondrogenic differentiation of rabbit chondrocytes in thermo-reversible hydrogel constructs blended with hyaluronic acid by in vivo test. J Biotechnol 2007,128:412-422. [25] Brown BN, Barnes CA, Kasick RT, Michel R, Gilbert TW, Beer-Stolz D, et al. Surface characterization of extracellular matrix scaffolds. Biomaterials 2010,31:428-437. [26] Bissell MJ, Hall HG, Parry G. How does the extracellular matrix direct gene expression? J Theor Biol 1982,99:31-68. [27] Boudreau N, Myers C, Bissell MJ. From laminin to lamin: regulation of tissue specific gene expression by the ECM. Trends Cell Biol 1995,5:1-4. [28] Zhang P, Luo XS, Wang HJ. Clinical transplantation of a tissue-engineered airway. Lancet 2009,373:718-718. [29] RemLinger NT, Czajka CA, Juhas ME, Vorp DA, Stolz DB, Badylak SF, et al. Hydrated xenogeneic decellularized tracheal matrix as a scaffold for tracheal reconstruction. Biomaterials 2010,31:3520-3526. [30] Gubbels SP, Richardson M, Trune D, Bascom DA, Wax MK. Tracheal reconstruction with porcine small intestine submucosa in a rabbit model. Otolaryngo Head-Neck Surg 2006,134:1028-1035. [31] Pribitkin EA, Ambro BT, Bloeden E, O'Hara BJ. Rabbit ear cartilage regeneration with a small intestinal submucosa graft. Laryngoscope 2004,114:1-19. [32] Lee M, Chang PCY, Dunn JCY. Evaluation of small intestinal submucosa as scaffolds for intestinal tissue engineering. J. Surg Res 2008,147:168-171. [33] Crapo PM, Wang YD. Small intestinal submucosa gel as a potential scaffolding material for cardiac tissue engineering. Acta Biomaterialia 2010,6:2091-2096. [34] Kim KS, Lee JY, Kang YM, Kim ES, Kim GH, Dal Rhee S, et al. Small intestine submucosa sponge for in vivo support of tissue-engineered bone formation in the presence of rat bone marrow stem cells. Biomaterials 2010,31:1104-1113. [35] Zhang YY, McNeill E, Tian H, Soker S, Andersson KE, Yoo JJ, et al. Urine derived cells are a potential source for urological tissue reconstruction. Journal of Urology 2008,180:2226-2233. [36] Horwitz E, Le BK, Dominici M, Mueller I, Slaper-Cortenbach I, Marini FC et al . Clarification of the nomenclature for MSC: the international society for cellular therapy position statement. Cytotherapy 2005,7:393- 5. [37] Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini FC, Krause DS, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006,8:315-317. [38] Mitrano TI, Grob MS, Carrion F, Nova-Lamperti E, Luz PA, Fierro FS, et al. Culture and characterization of mesenchymal stem cells from human gingival tissue. J Periodontol 2010,81:917-925. [39] Fournier BPJ, Ferre FC, Couty L, Lataillade JJ, Gourven M, Naveau A, et al. Multipotent progenitor cells in gingival connective tissue. Tissue Eng Part A 2010,16:2891-2899. [40] Matikainen T, Laine J. Placenta - an alternative source of stem cells. Toxicol Appl Pharmacol 2005,207:544-549. [41] Fukuchi Y, Nakajima H, Sugiyama D, Hirose I, Kitamura T, Tsuji K. Human Placenta-derived cells have mesenchymal stem/progenitor cell potential. Stem Cells 2004,22:649-658. [42] Igura K, Zhang X, Takahashi K, Mitsuru A, Yamaguchi S, Takashi TA. Isolation and characterization of mesenchymal progenitor cells from chorionic villi of human placenta. Cytotherapy 2004,6:543-553. [43] Chiou M, Xu Y, Longaker MT. Mitogenic and chondrogenic effects of fibroblast growth factor-2 in adipose-derived mesenchymal cells. Biochem Biophys Res Commun 2006,343:644-652. [44] Estes BT, Wu AW, Guilak F. Potent induction of chondrocytic differentiation of human adipose-derived adult stem cells by bone morphogenetic protein 6. Arthritis Rheum 2006,54:1222-1232. [45] Boumediene K, Vivien D, Macro M, Bogdanowicz P, Lebrun E, Pujol JP. Modulation of rabbit articular chondrocyte (RAC) proliferation by TGF-beta isoforms. Cell Prolif 1995,28:221-234. [46] de Haart M, Marijnissen WJ, van Osch GJ, Verhaar JA. Optimization of chondrocyte expansion in culture. Effect of TGF beta-2, bFGF and L-ascorbic acid on bovine articular chondrocytes. Acta Orthop Scand 1999,70:55-61. [47] Hunziker EB, Rosenberg LC. Repair of partial-thickness defects in articular cartilage: Cell recruitment from the synovial membrane. J Bone Jt Surg Am Vol 1996,78A:721-733. [48] Barry F, Boynton RE, Liu BS, Murphy JM. Chondrogenic differentiation of mesenchymal stem cells from bone marrow: Differentiation-dependent gene expression of matrix components. Exp Cell Res 2001,268:189-200. [49] Iwasaki M, Nakata K, Nakahara H, Nakase T, Kimura T, Kimata K, et al. Transforming growth factor-beta 1 stimulates chondrogenesis and inhibits osteogenesis in high density culture of periosteum-derived cells. Endocrinology 1993, 132:1603-1608. [50] Lin CH, Hsu SH, Huang CE, Cheng WT, Su JM. A scaffold-bioreactor system or a tissue-engineered trachea. Biomaterials 2009,30:4117-4126. [51] 林振寰,氣管及兩相軟硬骨組織工程之開發與應用研究,中興大學博士論文,民 98 [52] Bernardo ME, Emons JA, Karperien M, Nauta AJ, Willemze R, Roelofs H, et al. Human mesenchymal stem cells derived from bone marrow display a better chondrogenic differentiation compared with other sources. Connect Tissue Res 2007,48:132-140. [53] Hassan Afizah, Zheng Yang, James H.P. Hui, Hong-Wei Ouyang and Eng-Hin Lee. A comparison between the chondrogenic potential of human bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs) taken from the same donors. Tissue Eng 2007,13(4): 659-666. [54] Vidal MA, Robinson SO, Lopez MJ, Paulsen DB, Borkhsenious O, Johnson JR, et al. Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow. Vet Surg 2008,37:713-724. [55] 鄭舜榮,利用人類胎盤間葉幹細胞植入褐藻膠/奈米陶瓷複合材料結合可降解精密支架於軟骨組織工程之研究 ,中興大學碩士論文,民98 [56] 翁愫霙,利用脂肪幹細胞植入褐藻膠/奈米陶瓷複合材料結合可降解精密支架於軟骨組織工程之研究,中興大學碩士論文,民97 [57] Hsu SH, Huang GS, Lin SY, Feng F, Ho TT, Liao YC. Enhanced chondrogenic differentiation potential of human gingival fibroblasts by spheroid formation on chitosan membranes. Tissue Eng Part A 2011, [58] Tomar GB, Srivastava RK, Gupta N, Barhanpurkar AP, Pote ST, Jhaveri HM, et al. Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine. Biochem Biophys Res Com mun 2010,393:377-383. [59] Coelho CN, Kosher RA. A gradient of gap junctional communication along the anterior-posterior axis of the developing chick limb bud. Dev Biol 1991,148:529-535. [60] Miyanishi K, Trindade MC, Lindsey DP, Beaupre GS, Carter DR, Goodman SB, et al. Dose- and time-dependent effects of cyclic hydrostatic pressure on transforming growth factor-beta3-induced chondrogenesis by adult human mesenchymal stem cells in vitro. Tissue Eng 2006,12:2253-2262. [61] Yen BL, Huang HI, Chien CC, Jui HY, Ko BS, Yao M, et al. Isolation of multipotent cells from human term placenta. Stem Cells 2005,23:3-9. [62] Bernardo ME, Emons JA, Karperien M, Nauta AJ, Willemze R, Roelofs H, et al. Human mesenchymal stem cells derived from bone marrow display a better chondrogenic differentiation compared with other sources. Connect Tissue Res 2007,48:132-140. [63] Chang JC, Hsu SH, Chen DC. The promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate culture. Biomaterials 2009,30:6265-6275. [64] Zheng L, Fan HS, Sun J, Chen XN, Wang G, Zhang L, et al. Chondrogenic differentiation of mesenchymal stem cells induced by collagen-based hydrogel: an in vivo study. J Biomed Mater Res A 2010,93:783-792. [65] Aung T, Miyoshi H, Tun T, Ohshima N. Chondroinduction of mouse mesenchymal stem cells in three-dimensional highly porous matrix scaffolds. J Biomed Mater Res. 2002,61(1):75-82 [66] Jung Y, Chung YI, Kim SH, Tae G, Kim YH, Rhie JW, et al. In situ chondrogenic differentiation of human adipose tissue-derived stem cells in a TGF-beta1 loaded fibrin-poly(lactide-caprolactone) nanoparticulate complex. Biomaterials 2009,30(27):4657-64 [67] Matikainen T, Laine J. Placenta-an alternative source of stem cells. Toxicol Appl Pharmacol 2005,207:544-549. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/66319 | - |
dc.description.abstract | 本研究使用第二型膠原蛋白-透明質酸複合物 (type II collagen-hyaluronan, CII-HA)及豬小腸黏膜下層 (small intestinal submucosa, SIS) 作為誘導幹細胞軟骨化的基材,比較來自人類四種不同組織的間葉幹細胞軟骨分化的潛力,分別為骨髓間葉幹細胞 (bone marrow derived mesenchymal stem cells, BMCS),脂肪幹細胞 (adipose derived adult stem cells, ADAS),胎盤間葉幹細胞 (placenta-derived mesenchymal stem cells, PDMC) 及牙齦纖維母細胞 (gingival fibroblasts, GF)。在二維系統進行軟骨誘導分化14天後的結果顯示,胎盤間葉幹細胞最具有軟骨化潛力,其次為牙齦纖維母細胞,而後將此兩種細胞植入以冷凍乾燥法 (freeze-drying) 製成的第二型膠原蛋白-透明質酸複合支架及八層豬小腸黏膜下層進行三維的誘導軟骨分化培養,培養28天後結果顯示此兩種幹細胞植入第二型膠原蛋白-透明質酸複合支架具有較好的誘導軟骨化效果。最後在免疫缺乏小鼠皮下進行體內培養證實胎盤間葉幹細胞植入第二型膠原蛋白-透明質酸複合支架為最佳的組織工程軟骨。 | zh_TW |
dc.description.abstract | The chondrogenesis differentiation potential of mesenchymal stem cells (MSC) isolated from four different human tissues were compared on two biomaterials, type II collagen-hyaluronan composite (CII-HA) films and small intestinal submucosa (SIS) sheets. The four human MSCs were bone marrow-derived mesenchymal stem cells (BMSC), adipose-derived adult stem cells (ADAS), gingival fibroblasts (GF) and placenta-derived mesenchymal stem cells (PDMC). The CII-HA composite films or three-dimensional (3D) scaffold were fabricated in this study. Upon TGF-β3 induction, PDMC demonstrated the best chondrogenesis differentiation potential on both materials, followed by GF. PDMC and GF were further seeded in CII-HA composite scaffolds and 8-layer SIS scaffolds for evaluation of neocartilage formation in vitro. After 28 days, CII-HA composite scaffolds seeded with either MSCs were surfaced with a cartilaginous-like layer. Histology also showed better neocartilage formation when MSCs were grown in CII-HA composite scaffolds. NOD SCID mice subcutaneous implantation further confirmed that the combination of PDMC and CII-HA composite scaffolds promoted the formation of tissue-engineered cartilage. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T00:30:09Z (GMT). No. of bitstreams: 1 ntu-101-R98549018-1.pdf: 32012899 bytes, checksum: ed910c910ed9ab4f285390d1abdf7ac5 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 誌謝 I
摘要 II Abstract III 目錄 IV 表目錄 XI 圖目錄 XII 第一章 文獻回顧 1 1.1 軟骨組織[1] 1 1. 2 軟骨組織工程 2 1.3 成人間葉幹細胞 8 1.4 生長因子對軟骨分化的影響 9 1.5 研究構想 10 第二章 實驗方法與材料 11 2.1 人類牙齦纖維母細胞的分離與培養 11 2.2 人類胎盤間葉幹細胞的分離與培養 11 2.3 人類脂肪脂肪幹細胞的分離與培養 11 2.4 流式細胞儀分析 13 2.5 第二型膠原蛋白的萃取 14 2.6 第二型膠原蛋白-透明質酸複合膜的製作 14 2.7 第二型膠原蛋白-透明質酸複合支架的製作 15 2.8 SEM觀察 15 2.9 含水率測試 15 2.10 平面上的誘導軟骨分化 16 2.11 三維空間的誘導軟骨分化 16 2.12 Alcian blue染色 17 2.13 第二型膠原蛋白免疫化學染色 18 2.14 反轉錄聚合酶連鎖反應分析 (Reverse transcription polymerase chain reaction, RT-PCR) 18 2.14-1 RNA抽取 18 2.14-2 反轉錄反應 18 2.15 即時定量PCR 19 2.16 細胞數分析 20 2.17 葡萄糖胺聚醣 (GAG) 含量分析 20 2.18 第二型膠原蛋白含量分析 21 2.19 組織切片 21 2.20 蘇木紫-伊紅染色 (H&E stain) 21 2.21 三維空間的體內軟骨分化 22 第三章 實驗結果 23 3.1 第二型膠原蛋白-透明質酸複合膜及單層豬小腸黏膜下層之表面分析 23 3.2 第二型膠原蛋白-透明質酸複合支架及八層豬小腸黏膜下層之孔洞結構分析 23 3.3 流式細胞儀分析細胞表面抗原 24 3.4 二維軟骨化培養的染色分析 24 3.5 二維軟骨化培養的基因表現分析 24 3.6 三維誘導軟骨分化支架外觀觀察 25 3.7 三維誘導軟骨分化支架組織切片分析 25 3.7.1 H&E染色 25 3.7.2 Alcian blue染色 26 3.7.3 第二型膠原蛋白免疫化學染色 26 3.8 細胞於三維支架中誘導軟骨分化的DNA含量分析 27 3.9 細胞於三維支架中誘導軟骨分化的基質分泌之分析 27 3.10 細胞於三維支架中誘導軟骨分化的第二型膠原蛋白之分泌 28 3.11幹細胞於三維支架中進行體內培養之外觀觀察 28 3.12 幹細胞於三維支架中進行體內培養之組織切片分析 28 3.12.1 H&E染色 28 3.12.2 Alcian blue染色 29 3.12.3 第二型膠原蛋白免疫化學染色 29 3.13 幹細胞於三維支架中進行體內培養之DNA含量分析 29 3.14 幹細胞於三維支架中進行體內培養之GAG含量分析 30 13.15幹細胞於三維支架中進行體內培養之第二型膠原蛋白含量分析 30 第四章 討論 31 4.1 二維的誘導軟骨分化 31 4.2 三維的誘導軟骨分化 33 4.2.1 牙齦纖維母細胞與胎盤間葉幹細胞在三維支架中的體外誘導軟骨分化 33 4.2.2 牙齦纖維母細胞與胎盤間葉幹細胞在三維支架中的體內誘導軟骨分化之評估 36 第五章 結論 38 參考文獻 40 誌謝 I 摘要 II Abstract III 目錄 IV 表目錄 XI 圖目錄 XII 第一章 文獻回顧 1 1.1 軟骨組織[1] 1 1. 2 軟骨組織工程 2 1.3 成人間葉幹細胞 8 1.4 生長因子對軟骨分化的影響 9 1.5 研究構想 10 第二章 實驗方法與材料 11 2.1 人類牙齦纖維母細胞的分離與培養 11 2.2 人類胎盤間葉幹細胞的分離與培養 11 2.3 人類脂肪脂肪幹細胞的分離與培養 11 2.4 流式細胞儀分析 13 2.5 第二型膠原蛋白的萃取 14 2.6 第二型膠原蛋白-透明質酸複合膜的製作 14 2.7 第二型膠原蛋白-透明質酸複合支架的製作 15 2.8 SEM觀察 15 2.9 含水率測試 15 2.10 平面上的誘導軟骨分化 16 2.11 三維空間的誘導軟骨分化 16 2.12 Alcian blue染色 17 2.13 第二型膠原蛋白免疫化學染色 18 2.14 反轉錄聚合酶連鎖反應分析 (Reverse transcription polymerase chain reaction, RT-PCR) 18 2.14-1 RNA抽取 18 2.14-2 反轉錄反應 18 2.15 即時定量PCR 19 2.16 細胞數分析 20 2.17 葡萄糖胺聚醣 (GAG) 含量分析 20 2.18 第二型膠原蛋白含量分析 21 2.19 組織切片 21 2.20 蘇木紫-伊紅染色 (H&E stain) 21 2.21 三維空間的體內軟骨分化 22 第三章 實驗結果 23 3.1 第二型膠原蛋白-透明質酸複合膜及單層豬小腸黏膜下層之表面分析 23 3.2 第二型膠原蛋白-透明質酸複合支架及八層豬小腸黏膜下層之孔洞結構分析 23 3.3 流式細胞儀分析細胞表面抗原 24 3.5 二維軟骨化培養的染色分析 24 3.6 二維軟骨化培養的基因表現分析 24 3.7 三維誘導軟骨分化支架外觀觀察 25 3.8 三維誘導軟骨分化支架組織切片分析 25 3.8.1 H&E染色 25 3.8.2 Alcian blue染色 26 3.8.3 第二型膠原蛋白免疫化學染色 26 3.9 細胞於三維支架中誘導軟骨分化的DNA含量分析 27 3.10 細胞於三維支架中誘導軟骨分化的基質分泌之分析 27 3.11 細胞於三維支架中誘導軟骨分化的第二型膠原蛋白之分泌 28 3.12幹細胞於三維支架中進行體內培養之外觀觀察 28 3.13 幹細胞於三維支架中進行體內培養之組織切片分析 28 3.13.1 H&E染色 28 3.13.2 Alcian blue染色 29 3.13.3 第二型膠原蛋白免疫化學染色 29 3.14 幹細胞於三維支架中進行體內培養之DNA含量分析 29 3.15 幹細胞於三維支架中進行體內培養之GAG含量分析 30 3.16幹細胞於三維支架中進行體內培養之第二型膠原蛋白含量分析 30 第四章 討論 31 4.1 二維的誘導軟骨分化 31 4.2 三維的誘導軟骨分化 33 4.2.1 牙齦纖維母細胞與胎盤間葉幹細胞在三維支架中的體外誘導軟骨分化 33 4.2.2 牙齦纖維母細胞與胎盤間葉幹細胞在三維支架中的體內誘導軟骨分化之評估 36 第五章 結論 38 參考文獻 40 | |
dc.language.iso | zh-TW | |
dc.title | 人類間葉幹細胞於組織工程支架上軟骨分化能力之評估 | zh_TW |
dc.title | Evaluation of Chondrogenic Differentiation Potential of Human Mesenchymal Stem Cells Grown in Tissue Engineering Scaffolds | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 顏伶汝(Lin-Ju Yen),王佩華(Pei-Hwa Wang),張瑞芝,戴念梓 | |
dc.subject.keyword | 第二型膠原蛋白,透明質酸,豬小腸黏膜下層,胎盤間葉幹細胞,牙齦纖維母細胞,免疫缺乏小鼠, | zh_TW |
dc.subject.keyword | Type II collagen,Hyaluronan,Small intestinal submucosa,Placenta-derived mesenchymal stem cells,Gingival fibroblasts,NOD SCID mice, | en |
dc.relation.page | 70 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-02-13 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 高分子科學與工程學研究所 | zh_TW |
顯示於系所單位: | 高分子科學與工程學研究所 |
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