熱帶家蚊和地下家蚊幼蟲生長環境對其生長發育及雌蟲生殖之影響

Kai-Kuo Huang; 黃開國

請用此 Handle URI 來引用此文件： http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/66273

完整後設資料紀錄

DC 欄位	值	語言
dc.contributor.advisor	黃榮南(Rong-Nan Huang)
dc.contributor.author	Kai-Kuo Huang	en
dc.contributor.author	黃開國	zh_TW
dc.date.accessioned	2021-06-17T00:28:14Z	-
dc.date.available	2013-03-19
dc.date.copyright	2012-03-19
dc.date.issued	2012
dc.date.submitted	2012-02-14
dc.identifier.citation	蔡坤憲。1998。地下家蚊(culex pipiens molestus)在台灣之分布及生態特性之探討。私立東海大學生命科學系碩士論文。蔡坤憲、陳錦生。地下家蚊之生物學研究。1997，第九屆病媒防治技術研討會論文集，行政院環保署 pp.215-226。 Attardo, G. M., Hansen, I. A. and Raikhel, A. S. (2005) Nutritional regulation of vitellogenesis in mosquitoes: implications for anautogeny. Insect Biochem. Mol. Biol. 35, 661-675. Brown, M. R., Graf, R., Swiderek, K. M., Fendley, D., Stracker, T. H., Champagne, D. E., et al. (1998) Identification of a steroidogenic neurohormone in female mosquitoes. J. Biol. Chem. 273, 3967-3971. Eberle, M. W. and Reisen, W. K. (1986) Studies on autogeny in　Culex tarsalis: 1. Selection and genetic experiments. J. Am. Mosq. Control Assoc. 2, 38–43. Feinsod, F. M., Spielman, A. (1980) Nutrient-mediated juvenile hormone secretion in mosquitoes. J. Insect Physiol. 26, 113-117. Gillies, M. T. (1955) The pre-gravid phase of ovarian development in Anopheles funestus. Ann. Trop. Med. Parasitol. 49, 320-325. Gordadze, A. V., Korochkina, S. E., Zakharkin, S. O., Norton, A. L. and Benes, H. (1999) Molecular cloning and expression of two hexamerin cDNAs from the mosquito, Aedes aegypti. Insect Mol. Biol. 8, 55-66. Hagedorn, H. H. (1989) Physiological role of hemolymph ecdysteroids in the adult insect. Thieme Medical Publishers Inc. pp. 279–289. Hagedorn, H. H., Kerkut, G. A. and Gilbert, K. ( 1985) The role of ecdysteroids in reproduction. Pergamon Press. pp. 205–261. Hagedorn, H. H., O’Connor, J. D., Fuchs, M. S., Sage, B., Schlaeger, D. A. and Bohm, M. K. (1975) The ovary as a source of alpha-ecdysone in an adult mosquito. Proc. Natl. Acad. Sci. USA 72, 3255–3259. Harwood, R. F. (1966) The relationship between photoperiod and autogeny in Culex tarsalis (Diptera, Culicidae). Entomol. Exp. Appl. 9, 327–331. Kasai, S., Komagata, O., Tomita, T., Sawabe, K., Tsuda, Y., Kurahashi, H., et al. (2008) PCR-based identification of Culex pipiens complex collected in Japan. Jpn. J. Infect Dis. 61, 184-191. Klowden, M. J. (1997) Endocrine aspects of mosquito reproduction. Insect Biochem. Physiol. 35, 491-512. Lea, A. O. (1967) The medial neurosecretory cells and egg maturation in mosquitoes. J. Insect Physiol. 13, 419-429. Lien, J. C., Wu, T. N., Lin, C. H., Lin, C. C. and Weng, M. H. (1996) Occurrence of Culex pipiens issp. molestus Forskal, 1775 in northern Taiwan. Chin. J. Parasitol. 9, 19-26. O’Meara, G. F. (1979) Variable expressions of autogeny in three mosquito species. Int. J. Invert. Repro. 1, 253–261. Olejnicek, J. and Gelbic, I. (2000) Autogeny in Culex pipiens quinquefasciatus say. J. Vector Ecol. 25, 118-122. Provost-Javier, K. N., Chen, S. and Rasgon, J. L. (2010) Vitellogenin gene expression in autogenously Culex tarsalis. Insect Mol. Biol. 19, 423–429. Reisen, W., Milby, M., Reeves, W., Meyer, R. and Bock, M. (1983) Population ecology of Culex tarsalis (Diptera: Culicidae) in a　foothill environment of Kern County, California: temporal　changes in female relative abundance, reproductive status,　and survivorship. Ann. Entomol. Soc. Am. 76, 800–808. Reisen, W. K., Milby, M. M. and Bock, M. E. (1986) The effects of immature stress on selected events in the life history of Culex tarsalis. Mosq. News 44, 385–395. Roy, S. G., Hansen, I. A. and Raikhel, A. S. (2007) Effect of insulin and 20-hydroxyecdysone in the fat body of the yellow fever mosquito, Aedes aegypti. Insect Biochem. Mol. Biol. 37, 1317-1326. Su, T. and Mulla, M. S. (1997a) Nutritional reserves, body weight, and starvation tolerance of autogenous and anautogenous strains of Culex tarsalis (Diptera:Culicidae). J. Med. Entomol. 34, 68-73. Su, T. and Mulla, M. S. (1997b) Quantitative determination of free amino acids in the hemolymph of autogenous and anautogenous strains of Culex tarsalis (Diptera: Culicidae). J. Med. Entomol. 34, 729-734. Takken, W., Klowden, M. J. and Chambers, G. M. (1998) Effect of body size on host seeking and blood meal utilization in Anopheles gambiae sensu stricto (Diptera: Culicidae): the disadvantage of being small. J. Med. Entomol. 35, 639-645. Telang, A., Li, Y., Noriega, F. G. and Brown, M. R. (2006) Effects of larval nutrition on the endocrinology of mosquito egg development. J. Exp. Biol. 209, 645-655. Telang, A. and Wells, M. A. (2004) The effect of larval and adult nutrition on successful autogenous egg production by a mosquito. J. Insect Physiol. 50, 677-685. Telfer, W. H. and Kunkel, J. G. (1991) The function and evolution of insect storage hexamers. Annu. Rev. Entomol. 36, 205-228. Trpis, M. (1977) Autogeny in diverse populations of Aedes aegypti from East Africa. Tropenmed. Parasitol. 28, 77-82. Turell, M. J., Mores, C. N., Dohm, D. J., Komilov, N., Paragas, J., Lee, J. S., et al. (2006) Laboratory transmission of Japanese encephalitis and West Nile viruses by molestus form of Culex pipiens (Diptera: Culicidae) collected in Uzbekistan in 2004. J. Med. Entomol. 43, 296-300. Vinogradova, E. B. (1965) Autogenous development of ovaries in blood-sucking mosquitoes. Zoologische. Zhurnal. 44, 210-219. Weng, M. H., Lien, J. C., Lin, C. C. and Yao, C. W. (2000) Vector competence of Culex pipiens molestus (Diptera: Culicidae) from Taiwan for a sympatric strain of Japanese encephalitis virus. J. Med. Entomol. 37, 780-783. Zakharkin, S. O., Headley, V. V., Kumar, N. K., Buck, N. A., Wheeler, D. E. and Benes, H. (2001) Female-specific expression of a hexamerin gene in larvae of an autogenous mosquito. Eur. J. Biochem. 268, 5713-5722.
dc.identifier.uri	http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/66273	-
dc.description.abstract	蚊子可因生殖營養週期需要血液攝食與否而分為自生型與非自生型兩種，在雌蚊的生殖營養週期，最重要的階段為卵黃蛋白合成時期，當中又以卵黃蛋白原 (vitellogenin, Vg )基因為該時期最主要且有最高表現量的關鍵基因，為了要瞭解自生型與非自生型的Vg基因表現之異同，嘗試以熱帶家蚊（Culex quinquefasciatus）與其相似卻具自生型特性之地下家蚊 (Culex pipiens molestus)作為研究材料，分析兩品種之間的Vg基因表現的模式。同時也針對蚊幼蟲生長條件的控制及生長調節藥劑（tebufenozide）處理，探討是否能影響其成蟲生殖策略的改變，使其從非自生型轉變成自生型蚊種。結果顯示，提高幼蟲飼養的營養、降低幼蟲飼養密度，以及調整飼養光照條件，確實能增加蛹體體積，以及增大成蟲體型，但仍無法改變其生殖型式。而以 RT-PCR技術偵測蚊子的兩種Vg基因表現，結果顯示無論是地下家蚊或熱帶家蚊，其VgC1和VgC2在幼蟲及蛹期都有少量表現，但在血液攝取後及產卵前，VgC1和VgC2會有明顯的大量表現，產卵後表現量即大副降低，回復到吸血前的少量表現。然而在VgC1大量表現時期，其cDNA會有部分序列被刪除 (delete)，這個現象是否與其生殖機制調節有關，有待進一步深入研究。	zh_TW
dc.description.abstract	Autogenous Culex pipiens molestus is a good model for better understanding the relationship between oviposition and non-blood feeding signal. Vitellogenesis is the central step which involves the synthesis and secretion of yolk protein precursors (YPP) on a massive scale by the fat body in mosquito reproduction. In this thesis, the influence of larval rearing environment and Tebufenozide treatment on the growth of mosquito were compared by using two different mosquito species, autogenous (Cx. pipiens molestus) and anautogenous (Culex quinquefasciatus). Subsequently, RT-PCR analysis of Vg mRNA extracted from Cx. quinquefasciatus and Cx. pipiens molestus mosquito during a reproductive cycle will be presented. The results showed that tebufenozide treatment, abounding larval nourishment and low larval intensity have significantly impacted on the growth character and fecundity in both mosquitoes. However, rearing environment and Tebufenozide treatment have no effect on the anautogenous of mosquito. Moreover, vitellogenin genes (VgC1, VgC2) were only highly expressed after a bloodmeal. However, VgC1 sequence showed a 61 bp deletion during the period of high expression The effect of VgC1 sequence deletion on the reproduction of these two mosquitoes is currently unknow and deserves further investigation.	en
dc.description.provenance	Made available in DSpace on 2021-06-17T00:28:14Z (GMT). No. of bitstreams: 1 ntu-101-R97632014-1.pdf: 1563317 bytes, checksum: 9a5a6110db9c3c74a7d813d88972f49f (MD5) Previous issue date: 2012	en
dc.description.tableofcontents	致謝.......................................................................................................i 中文摘要...............................................................................................ii 英文摘要..............................................................................................iii 目錄.......................................................................................................iv 圖目錄..................................................................................................viii 表目錄....................................................................................................ix 壹、緒言..............................................................................................1 貳、往昔研究.......................................................................................4 一、自生型蚊種和非自生型的蚊種..................................................4 二、雌蚊的卵黃蛋白合成 (vitellogenesis) .......................................5 三、胺基酸在雌蚊生殖週期中所扮演的腳色....................................6 四、地下家蚊生殖產卵特性..............................................................7 參、材料與方法.....................................................................................9 一、幼蟲生長條件與成蟲生殖性能之關係........................................9 （一）蚊蟲來源與飼養.....................................................................9 （二）不同飼料種類餵食對於幼蟲生長的影響..............................9 （三）不同飼料量餵食對於幼蟲生長的影響.................................10 （四）地下家蚊幼蟲養份攝取情形與成蟲吸血行為之關連..........10 （五）生長空間密度對於幼蟲生長之影響....................................11 （六） Tebufenozide處理幼蟲對於成蟲之影響..................................11 （七）光照強度對成蟲的壽命影響...................... ...................................12 二、熱帶家蚊與地下家蚊卵黃蛋白基因的表現...............................12 (一) 引子的測試..........................................................................13 (二) 卵黃蛋白基因於地下家蚊及熱帶家蚊各生長期之表現.......13 (三) Tebufenozide對於Vg基因表現的影響................................13 1. RNA的萃取.......................................................................14 2. cDNA的合成.....................................................................14 2.1 primer的設計.................................................................14 2.2反轉錄酶反應 (Reverse Transcriptase, RT) ....................15 2.3聚合酶鏈鎖反應 (Polymerase chain reaction, PCR).........16 3. PCR產物的分析.................................................................16 4. PCR產物定序.....................................................................16 4.1 DNA膠體純化萃取..........................................................16 4.2 DNA接合反應(Ligation) .................................................17 4.3 質體轉型(Transformation) ..............................................17 三、以二維電泳技術分析地下家蚊行自生型生殖週期時，產卵前後之腹部蛋白變化..................................................................................17 (一) 雌蚊腹部取樣與蛋白質製備.................................................18 (二) 二維電泳...............................................................................18 肆、結果.................................................................................................19 一、幼蟲生長條件與成蟲生殖性能之關連.........................................19 (一) 不同飼料種類餵飼對於幼蟲生長的影響.....................................19 (二) 不同飼料量餵食對於幼蟲生長的影響....................................19 (三) 地下家蚊幼蟲養份攝取情形與成蟲吸血行為之關連....................20 　　 (四) 生長空間密度對於幼蟲生長的影響.......................................21 （五）Tebufenozide處理幼蟲對於成蟲之影響................................22 (六) 光照強度對成蟲的影響............................................................23 二、熱帶家蚊與地下家蚊卵黃蛋白基因的表現.................................23 (一) 引子對的測試..........................................................................23 (二) 熱帶家蚊的Vg基因表現模式.................................................25 (三) 地下家蚊的Vg基因表現模式.................................................25 (四) Tebufenozide對於熱帶家蚊Vg基因表現的影響....................26 三、以二維電泳技術分析地下家蚊行自生型生殖週期時，產卵前後之腹部蛋白變化............................................................................27 伍、討論................................................................................................29 陸、參考文獻........................................................................................34 柒、附錄...............................................................................................37 附錄一、血液攝食驅動卵黃生成示意圖..............................................37 附錄二、以Ace-orthologous acetylcholinesterase (Ace) 基因進行熱帶家蚊及地下家蚊之鑑定............................................................38
dc.language.iso	zh-TW
dc.subject	地下家蚊	zh_TW
dc.subject	熱帶家蚊	zh_TW
dc.subject	非自生型	zh_TW
dc.subject	卵黃蛋白原	zh_TW
dc.subject	卵黃蛋白原	zh_TW
dc.subject	熱帶家蚊	zh_TW
dc.subject	非自生型	zh_TW
dc.subject	地下家蚊	zh_TW
dc.subject	自生型	zh_TW
dc.subject	自生型	zh_TW
dc.subject	vitellogenin	en
dc.subject	Culex quinquefasciatus	en
dc.subject	Culex pipiens molestus	en
dc.subject	Autogenous	en
dc.subject	Anautogenous	en
dc.subject	vitellogenin	en
dc.subject	Culex quinquefasciatus	en
dc.subject	Culex pipiens molestus	en
dc.subject	Autogenous	en
dc.subject	Anautogenous	en
dc.title	熱帶家蚊和地下家蚊幼蟲生長環境對其生長發育及雌蟲生殖之影響	zh_TW
dc.title	The effect of larval environments on the development and female reproduction in Culex quinquefasciatus & Culex pipiens molestus	en
dc.type	Thesis
dc.date.schoolyear	100-1
dc.description.degree	碩士
dc.contributor.oralexamcommittee	杜武俊(Wu-Chun Tu),許如君(Ju-Chun Hsu)
dc.subject.keyword	熱帶家蚊,地下家蚊,自生型,非自生型,卵黃蛋白原,	zh_TW
dc.subject.keyword	Culex quinquefasciatus,Culex pipiens molestus,Autogenous,Anautogenous,vitellogenin,	en
dc.relation.page	39
dc.rights.note	有償授權
dc.date.accepted	2012-02-14
dc.contributor.author-college	生物資源暨農學院	zh_TW
dc.contributor.author-dept	昆蟲學研究所	zh_TW
顯示於系所單位：	昆蟲學系

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