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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65764完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊台鴻(Tai-Horng Young) | |
| dc.contributor.author | Junichi Yoriki | en |
| dc.contributor.author | 余力純一 | zh_TW |
| dc.date.accessioned | 2021-06-17T00:11:15Z | - |
| dc.date.available | 2012-07-19 | |
| dc.date.copyright | 2012-07-19 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-07-13 | |
| dc.identifier.citation | Reference
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65764 | - |
| dc.description.abstract | 現今已證明關節滑膜裡存在有間葉幹細胞。細胞與細胞之間的聯繫對間葉幹細胞的軟骨分化非常重要。滑膜細胞養在 TCPS 上的形態是類似纖維母細胞。細胞養在幾丁聚醣基材上的時候，細胞會聚集成球。養在幾丁聚醣的細胞在低強度超音波(LIUS)刺激的環境下，細胞聚集的情形更明顯。當滑膜細胞養在 TCPS 上的情況下不會有軟骨分化，但在幾丁聚醣上成球的細胞，如 Safranin O 染色所示，有軟骨分化的傾向。再來用LIUS刺激的細胞比養在幾丁聚醣更加強糖胺聚醣的表現。在本研究裡，測軟骨分化有關的基因表現。在培養時間點前面的時候，養在幾丁聚醣上或養在幾丁聚醣加LIUS，sox9 基因表現各增加了1.8倍和2.7倍。這些結果告訴我們LIUS刺激促進滑膜細胞透過 sox9 分化成軟骨細胞。 | zh_TW |
| dc.description.abstract | Synovial membrane has been shown to contain mesenchymal stem cells. It is very important for stem cells to control cell-cell interaction for chondrogenic induction. Synovial cells cultured on TCPS exhibit normal fibroblast-like morphology. Cells formed spheroids when cultured on chitosan-coated substrate. Cells cultured on chitosan under low-intensity ultrasound (LIUS) stimulation formed spheroids larger than that on chitosan alone. Synovial cells cultured on TCPS could not undergo chondrogenic differentiation but cells cultured on chitosan differentiated into chondrocytes due to their spheroid formation as assessed by Safranin O staining. Furthermore, LIUS (0.1 W, 1MHz, duty cycle 10 %) applied every day enhanced more GAGs expression than cultured on chitosan only. In this study, the expression of chondrogenesis marker genes was observed. Gene expression of sox9 was increased by 1.8 and 2.7 folds by cells cultured on chitosan alone and chitosan with LIUS stimulation, respectively at early stage of cell culture. These results indicate that LIUS enhances sox9 mediated chondrogenic differentiation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T00:11:15Z (GMT). No. of bitstreams: 1 ntu-101-R99543082-1.pdf: 1517311 bytes, checksum: d71c0990492307de8ed0858559e4ea72 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | Contents
Abstract I Contents III Figures V Tables VIIII Chapter 1. Introduction 1 Chapter 2. Backgorund 3 2-1 Cell Source (synovial membrane) 3 2-2 Effect of low-intensity ultrasound (LIUS) stimulation 4 2-2-1 What is low intensity ultraosund (LIUS) 4 2-2-2 Effcets of LIPUS on chondrogenesis of MSCs 5 2-2-3 Effects of LIUS on the in vivo catilage regeneration and osteoarthritis 7 2-3 Biomaterials 10 2-3-1 Chitosan 10 Chapter 3. Materials and Methods 12 3-1 Materials 12 3-2 Experiment apparatus 15 3-3 Preparation of solution 17 3-4 Preparation of biomaterials 19 3-5 Ultraosound stimulation 19 3-6 Cell isolation and culture 22 3-7 Gene expression: Real Time RT - PCR analysis 22 3-8 GAGs staining - Safranin O stain 23 Chapter 4. Results and Discussions 26 4-1 Selection of LIUS condition 26 4-2 Cell morphology 27 4-3 GAGs expression of synovial cells 28 4-4 Chondrogenic specific gene expression 29 Chapter 5. Conclusion 31 Reference 32 Appindex 40 | |
| dc.language.iso | en | |
| dc.subject | 低強度超音波 | zh_TW |
| dc.subject | 軟骨分化 | zh_TW |
| dc.subject | TGF-beta1 | zh_TW |
| dc.subject | sox9 | zh_TW |
| dc.subject | 滑膜細胞 | zh_TW |
| dc.subject | 幾丁聚醣 | zh_TW |
| dc.subject | chondrogenic differentiation | en |
| dc.subject | chitosan | en |
| dc.subject | low-intensity ultrasound (LIUS) | en |
| dc.subject | TGF-beta 1 | en |
| dc.subject | sox9 | en |
| dc.subject | synovial cells | en |
| dc.title | 低強度超音波對於滑膜細胞培養在幾丁聚醣基材上
軟骨分化之研究 | zh_TW |
| dc.title | Effects of low-intensity ultrasound (LIUS) on chondrogenic differentiation of synovial cells cultured on chitosan substrate | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 王至弘(Jyh-Horng Wang) | |
| dc.contributor.oralexamcommittee | 李亦淇 | |
| dc.subject.keyword | 低強度超音波,幾丁聚醣,滑膜細胞,sox9,TGF-beta1,軟骨分化, | zh_TW |
| dc.subject.keyword | low-intensity ultrasound (LIUS),chitosan,synovial cells,sox9,TGF-beta 1,chondrogenic differentiation, | en |
| dc.relation.page | 54 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-07-13 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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