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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 洪挺軒(Ting-Hsuan Hung) | |
| dc.contributor.author | Ying-Hua Chen | en |
| dc.contributor.author | 陳映樺 | zh_TW |
| dc.date.accessioned | 2021-06-16T23:36:29Z | - |
| dc.date.available | 2013-08-01 | |
| dc.date.copyright | 2012-08-01 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-07-26 | |
| dc.identifier.citation | 1. 楊秀修。1997。柑橘萎縮病毒之感染生態。國立臺灣大學碩士論文。
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Vela, C., Cambra, M., Cortes, E., Moreno, P., Miguet, S.G., Perez de San Roman, C. and Sanz, A. 1986. Production and characterisation of monoclonal antibodies specific for citrus tristeza virus and their use for diagnosis. J. Gen. Virol. 67, 91–96. 110. Webber, H. J. 1925. A comparative study of the citrus industry of South Africa. So. Africa Dept. Agr. Bul. 6. 106pp. 111. Yokomi, R.K. and Garnsey, S.M. 1987. Transmision of citrus tristeza virus by A. gossypii and A. citricola in Florida. Phytophylactica, 19, 169–172. 112. Yokomi, R.K., Lastra, R., Stoetzel, M.B., Damsteegt, V.D., Lee, R.F., Garnsey, S.M., Gottwald, T.R., Rocha-Pena, M.A. and Niblett, C.L. 1994. Establishment of the brown citrus aphid (Homoptera, Aphididae) in Central-America and the Caribbean Basin and transmission of citrus tristeza virus. J. Econ. Entomol. 87, 1078–1085. 113. Yoshida, T. 1996. Graft compatibility of Citrus with plants in the Aurantoidea and their susceptibility to citrus tristeza virus. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65322 | - |
| dc.description.abstract | 柑橘萎縮病毒 (Citrus tristeza virus = CTV) 屬於Closterovirus屬之長絲狀病毒，影響世界柑橘生產甚鉅，也是目前危害台灣柑橘果園最重要的病毒病害。CTV可藉由嫁接、高壓等無性繁殖而傳播，田間還可經由媒介蚜蟲來傳播蔓延，因此台灣柑橘植株受到感染的比例高達90%以上。受到CTV感染的柑橘植株會使得果實變小、果肉硬化汁少、及品質降低等，對於柑橘產業而言，是一項很嚴重的問題。由於CTV存在已久，病毒演化的時間相當長，故其病毒系統具有相當的多樣性，各個系統對不同柑橘品種，其致病力亦有差異，例如：CTV苗黃系統 (seedling yellows strain，CTV/SY) 普遍感染寬皮柑及橙類影響品質與產量頗多，但無法成功感染柚類，柚類莖陷系統 (pummelo stem-pitting strain，CTV-Pum/SP) 卻可以在柚類上產生嚴重的萎縮病徵。本研究從台灣柑橘重要產區選定了六個分離株進行生物性鑑別，分別為柚類莖陷強毒系統 (CTV-Pum/SP/T1) 與弱毒系統 (CTV-Pum/M/T5)、甜橙莖陷強毒系統 (CTV-SwO/SP/T7) 與弱毒系統(CTV-SwO/M/T51)、以及寬皮柑莖陷強毒系統 (CTV-Man/SP/T46) 與弱毒系統(CTV-Man/M/T2)，以文旦柚、甜橙(柳丁)、椪柑、尤利加檸檬、墨西哥萊姆、Yuzu、粗皮檸檬以及廣東檸檬作為鑑別植物，觀察不同的CTV分離株在各個指示植物中之表現情形；接種後六個月，CTV-Pum/SP/T1大幅地影響了文旦柚的生長，但是甜橙、椪柑及尤利加檸檬則不受影響，CTV-Pum/M/T5則幾乎對各種指示植物都不表現病徵；而CTV-SwO/SP/T7除了對甜橙有影響之外，也會使文旦柚、尤利加檸檬及墨西哥萊姆產生病徵，CTV-SwO/M/T51則會使文旦柚皮層大幅增厚，並有輕微的莖陷病徵；CTV-Man/SP/T46除了對寬皮柑有影響之外，也會對文旦柚、柳橙、尤利加檸檬等造成危害，而CTV-Man/M/T2則會使文旦產生甚嚴重之木質部凹陷病徵。另一方面，基於近年來柚類莖陷系統似乎轉趨嚴重，文旦柚受害情況增加許多，故選擇CTV-Pum/SP/T1及CTV-Pum/M/T5進行病毒基因體全長解序，發現CTV-Pum/SP/T1與NCBI GenBanK中來自美國夏威夷發表的HA18-9 (GenBank accession No.GQ454869) 有高達96%的相似度，而CTV-Pum/M/T5則與同樣來自美國夏威夷的HA16-5 (GenBank accession No.GQ454870) 有93.5%的相似度，但CTV-Pum/SP/T1與CTV-Pum/M/T5經過分析並無序列重組的情形。另外，經由比對，發現CTV-Pum/SP/T1之ORF p1a第2308個核苷酸處與美國所發表的甜橙苗黃強毒系統T36 (GenBank accession No. EU937521) 同樣有一段大約20個核苷酸的TCT重複序列，而CTV-Pum/M/T5與甜橙苗黃弱系統T30 (GenBank accession No. EU937520) 則無此情形。 | zh_TW |
| dc.description.abstract | Citrus tristeza virus (CTV) (genus closterovirus, family closteroviridae) causes important viral diseases of citrus worldwide. In Taiwan, above 90% of the citrus trees are infected with CTV. CTV can be transmitted by a few aphid species, and also by graft-transmission. Citrus trees infected with CTV become weak and stunting, and the fruit become small and hard. In this article, we choose six strains of CTV in Taiwan and inoculating to differential citrus cultivars including Wentan pummelo, Liucheng sweet orange, Pongkan mandarin, Lankat mandarin, Eureka lemon, Mexican lime, Yuzu, Rough lemon and Rangpur lime. After six months, CTV-Pum/SP/T1 caused severe symptoms on Wentan pummelo, but not on any other citrus cultivars; CTV-Pum/M/T5 did not cause any symptoms. CTV-SwO/SP/T7 caused severe symptoms on Liucheng sweet orange, even on Wentan pummelo and Pongkan mandarin it caused leaf atrophy; CTV-SwO/M/T51 did not cause any symptoms on Liucheng sweet orange, but cause symptom on Wentan pummelo. CTV-Man/SP/T46 caused severe symptoms not only in Pongkan mandarin, but also on Wentan pummelo and Eureka lemon; CTV-Man/M/T2 did not cause any symptoms on Pongkan mandarin, but it caused severe stem pitting on Wentan pummelo. Besides, we sequenced the complete grnome of CTV-Pum/SP/T1 and CTV-Pum/M/T5, and got whole genome sequences of them. Sequence analysis revealed CTV-Pum/SP/T1 was most close to HA18-9 (GenBank accession No.GQ454869) reported from Hawaii, US, and CTV-Pum/M/T5 was most close to HA16-5 (GenBank accession No.GQ454870) reported from Hawaii. Meanwhile, we found that there were TCT repeat sequences at ORF P1a in CTV-Pum/SP/T1 and CTV-SwO/SY/T36 (GenBank accession No. EU937521), but not in genome of CTV-Pum/M/T5 and CTV-SwO/M/T30 (GenBank accession No. EU937520) . | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T23:36:29Z (GMT). No. of bitstreams: 1 ntu-101-R99633001-1.pdf: 24270872 bytes, checksum: 9b536e4c713c6c3839e73d57d5d1f3e6 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 中文摘要 ii ABSTRACT iv 目錄 vi 表目錄 viii 圖目錄 ix 壹、前言 1 貳、前人研究 3 一、 柑橘萎縮病毒之發現與歷史 3 二、 柑橘萎縮病毒引起之病徵與系統分類 5 三、 柑橘萎縮病毒之寄主範圍 6 四、 柑橘萎縮病毒之發生與傳播 7 五、 柑橘萎縮病毒之分類地位與基因體研究 8 六、 柑橘萎縮病毒之診斷與偵測 9 七、 柑橘萎縮病毒之防治 9 參、 材料與方法 12 一、 柑橘萎縮病毒之病理性鑑別 12 A. 試驗植物準備及柑橘萎縮病毒之來源 12 B. 柑橘萎縮病毒之接種 12 C. 夾層免疫酵素聯結法 (DAS-ELISA) 檢測柑橘萎縮病毒 12 D. 接種柑橘萎縮病毒病株減少生長之計算 13 二、 柑橘萎縮病毒之分子性鑑別 13 A. 柑橘萎縮病毒核酸抽取 13 B. 柑橘萎縮病毒柚類莖陷強毒與弱毒系統之全長解序 14 C. 設計病毒系統專一性引子對 20 D. 柑橘萎縮病毒Real-Time RT-PCR偵測技術之建立 20 肆、結果 23 一、 柑橘萎縮病毒之病理性鑑別 23 A. 不同柑橘萎縮病毒系統之病徵表現 23 B. 六個柑橘萎縮病毒系統在不同柑橘品種中之增殖情形 24 C. 六個柑橘萎縮病毒系統在不同柑橘品種中使植株減少生長之情形 24 二、 柑橘萎縮病毒之分子性鑑別 25 A. 柑橘萎縮病毒柚類莖陷強毒系統及弱毒系統全長解序與序列比對 25 B. 柑橘萎縮病毒柚類莖陷系統之重組分析與親緣分析 25 C. 柑橘萎縮病毒之通用引子對 25 D. 柑橘萎縮病毒之系統專一性引子對 26 E. 即時定量反轉錄聚合酶反應Real-Time RT-PCR 26 伍、討論 27 一、 柑橘萎縮病毒之病理性鑑別 27 A. 試驗植物之準備 27 B. 柑橘萎縮病毒系統病理性鑑別 27 二、 柑橘萎縮病毒分子性鑑別 29 A. 柑橘萎縮病毒柚類莖陷強毒系統與弱毒系統之全長解序與序列分析 29 B. 柑橘萎縮病毒之偵測 30 C. 即時定量反轉錄聚合酶連鎖反應Real-Time RT-PCR 31 陸、結語 32 柒、參考文獻 33 捌、表 44 玖、圖 56 拾、附錄 67 | |
| dc.language.iso | zh-TW | |
| dc.title | 不同的臺灣柑橘萎縮病毒系統之病理性鑑別與基因體分析 | zh_TW |
| dc.title | Pathological categorization and genomic analysis for different strains of Citrus tristeza virus in Taiwan | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 蘇鴻基(Hong-Ji Su) | |
| dc.contributor.oralexamcommittee | 葉信宏(Hsin-Hung Yeh) | |
| dc.subject.keyword | 柑橘萎縮病毒,系統,病徵, | zh_TW |
| dc.subject.keyword | Citrus tristeza virus,strains,symptom, | en |
| dc.relation.page | 115 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-07-26 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
| 顯示於系所單位： | 植物病理與微生物學系 | |
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