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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 吳嘉文 | |
dc.contributor.author | Yung-He Liang | en |
dc.contributor.author | 梁永和 | zh_TW |
dc.date.accessioned | 2021-06-16T23:32:20Z | - |
dc.date.available | 2014-08-01 | |
dc.date.copyright | 2012-08-01 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-07-27 | |
dc.identifier.citation | (1) Slowing, I. I.; Vivero-Escoto, J. L.; Wu, C.-W.; Lin, V. S. Y. Advanced Drug Delivery Reviews 2008, 60, 1278.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65243 | - |
dc.description.abstract | A new drug nanovehicle consisting of inorganic calcium phosphate (CaP) nanoparticle as core and organic alginate as shell (denoted as CaP@alginate) has been successfully synthesized via a pre-gel method and applied for pH-sensitive drug delivery systems (DDS). The CaP@alginate nanocomposite contains both advantages of CaP and alginate, where CaP provides pH-responsive degradability and alginate provides excellent biocompatibility and COOH functionality. Through the sequent addition of CaCl2 and phosphate solutions into alginate solution, CaP@alginate nanocomposites with controllable particle sizes (ranging from 160 to 650 nm) were obtained, and the core-shell structure of the CaP@alginate was observed under transmission electron microscopy (TEM). Rhodamine 6G (R6G), a positively charged and water-soluble dye, was selected as a model drug for pH-sensitive DDS. R6G was encapsulated in the nanocomposites during synthesis, and its loading efficiency can reach up to ca. 63.0%. The in vitro release behavior of the loaded R6G at different pH values was systematically studied, and the results indicated that more R6G molecules were released at lower pH conditions. For example, after release for 8 hours, the release amount of R6G at pH 2.0 was 2.53-fold of that at pH 7.4. We attributed this pH-sensitive release behavior to the dissolution of the CaP core at acidic conditions, and this pH-responsive behavior was expected to enhance the selective delivery of internal cargos. The results of MTT assay and confocal laser scanning microscopes indicated CaP@alg were successfully uptaken by HepG2 cells without apparent cytotoxicity. The CaP@alginate nanocomposites synthesized in this report show great potential as drug nanovehicles with high biocompatibility, specific targeting, and pH-responsible features for intracellular DDS in the future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T23:32:20Z (GMT). No. of bitstreams: 1 ntu-101-R99524046-1.pdf: 5567750 bytes, checksum: d05a104523583eacb0e05355011f189b (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 誌謝 1
Abstract 2 摘要 3 Table of Content 4 List of Figures 6 List of Tables 9 1. Introduction 10 1.1. Drug delivery system 10 1.2. Nanoparticles as drug carrier 10 1.3. Polymers in drug delivery 13 1.3.1. Polymer therapeutics (polymer-based drugs) 13 1.3.2. Natural polysaccharides as drug carriers 14 1.4. Alginate – a natural polysaccharide 15 1.4.1. Properties of alginate 15 1.4.2. Ionic crosslinking 16 1.4.3. Application of alginate 16 1.4.4. Pre-gel state of alginate 17 1.4.5. Alginate nanoparticles synthesized from pre-gel method 18 1.5. Calcium phosphate 21 2. Experimental 24 2.1. Materials 24 2.2. Synthesis of the nanoparticles 24 2.3. Characterization 25 2.4. Determination of the drug loading efficiency 26 2.5. In vitro release 27 2.6. Cell culture 27 2.7. MTT assay 27 2.8. Confocal laser scanning microscopy (CLSM) 28 3. Result and discussion 29 3.1. Preparation and characterization of the nanoparticles 29 3.1.1. Synthesis of the nanoparticles 29 3.1.2. Formation mechanism 47 3.2. Cytotoxicity 49 3.3. Drug loading 50 3.4. In vitro release 56 3.5. Cellular Uptake 60 4. Conclusion 64 5. Future work 65 6. Reference 67 7. Appendix 72 Appendix A. Determination of the gelation point of alginate 72 Appendix B. Particle size distributions from DLS. 75 Appendix C. Comparison between the pellet and supernatant 81 Appendix D. CLSM images of cells treated with R6G or (R6G-CaP)@alginate 83 Appendix E. Removal of the CaP core for hollow alginate nanoparticles 85 | |
dc.language.iso | en | |
dc.title | 以預凝膠法製備磷酸鈣/海藻膠之核/殼奈米粒子並應用於酸鹼應答型的藥物釋放 | zh_TW |
dc.title | Synthesis of calcium phosphate/alginate core/shell nanoparticles (CaP@alginate) through pre-gel method as pH-responsive drug carriers | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張雍,游佳欣,蔡偉博,林?輝 | |
dc.subject.keyword | 海藻膠,磷酸鈣,奈米粒子,藥物釋放,酸鹼應答,預凝膠, | zh_TW |
dc.subject.keyword | Alginate,Calcium Phosphate,Nanoparticle,Drug Delivery,pH-responsive,Pre-gel, | en |
dc.relation.page | 88 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-07-30 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
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