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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 曲芳華(Fang-Hua Chu) | |
| dc.contributor.author | Chi-Hsiang Wen | en |
| dc.contributor.author | 文起祥 | zh_TW |
| dc.date.accessioned | 2021-06-16T23:07:14Z | - |
| dc.date.available | 2014-08-09 | |
| dc.date.copyright | 2012-08-09 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-05 | |
| dc.identifier.citation | 劉棠瑞、廖日京(1980)樹木學。臺灣商務印書館。408頁。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64911 | - |
| dc.description.abstract | 本研究以臺灣原生樹種楓香（Liquidambar formosana Hance）為材料，使用微矩陣（microarray）偵測楓香綠葉、紅葉及黃葉微核醣核酸的表現。由分析結果得知miR164、miR828、miR157及miR172等微核醣核酸（microRNA）家族有明顯之差異表現，經由可能的調控途徑推衍，判知miR164、miR828、miR157及miR172可能參與楓香葉老化、葉變色及葉片生長發育之調控。此外，配合葉變色相關基因pheophorbide a oxygenase（PaO）、dihydroflavanoid reductase（DFR）及anthocyanidin synthase（ANS）於不同時期之表現情形、啓動子序列分析資料及葉片色素含量之測定，確認微核醣核酸於落葉性木本植物葉老化及葉變色調控之作用。本研究為首篇提出微核醣核酸參與調控秋季葉老化及葉變色之研究報告。 | zh_TW |
| dc.description.abstract | The leaves of different developmental stage of Formosan sweetgum (Liquidambar formosana Hance), a native species of Taiwan, were analyzed by using microarray to detect the expression of microRNA genes, including green, red and yellow leaves. The identified microRNA genes miR164, miR828, miR157 and miR172 were involved in Formosan sweetgum leaf senescence, coloration and development. Couple with chlorophyll quantification, anthocyanin absorbance measurement, promoter analysis and genes expression of coloration associated genes pheophorbide a oxygenase (PaO), dihydroflavanoid reductase (DFR) and anthocyanidin synthase (ANS), confirm that the current model of microRNA genes for leaf senescence and coloration did occur in Formosan sweetgum. This is the first study illustrate microRNAs as regulators in autumn leaf senescence and coloration. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T23:07:14Z (GMT). No. of bitstreams: 1 ntu-101-R99625018-1.pdf: 1961149 bytes, checksum: 6be7e8adce25ea1c2638f3c7256954e3 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 口試委員會審定書……………………………………………………………………i
誌謝…………………………...………………………………………………………ii 中文摘要……………………………………………………………………………..iii 英文摘要……………………………………………………………………………..iv 縮寫表…………………………………………………………………….…………..v 目錄..............................................................................................................................vi 圖目錄..........................................................................................................................xi 表目錄........................................................................................................................xiii 第一章 緒論 .............................................................................................................1 第一節 前言........................................................................................................1 第二節 研究背景................................................................................................3 2.1 秋季葉變色.................................................................................................4 2.1.1葉老化...................................................................................................4 2.1.2 葉變色..................................................................................................8 2.2 微核醣核酸...............................................................................................14 2.3 楓香...........................................................................................................20 第三節 實驗概念及架構..................................................................................22 第二章 實驗材料與方法.........................................................................................24 第一節 實驗材料..............................................................................................24 1.1 實驗物種...................................................................................................24 1.2 實驗樣本...................................................................................................24 第二節 實驗方法..............................................................................................25 2.1 生長發育時期之劃分...............................................................................25 2.1.1 楓香葉片之影像檔建立....................................................................25 2.1.2 葉片色素之定量及測量....................................................................25 2.1.2.1 葉綠素、類胡蘿蔔素之定量.....................................................26 2.1.2.2 花青素、紫外光吸收色素之測量.............................................26 2.1.3 葉變色相關基因表現量觀察............................................................27 2.1.3.1 葉變色相關基因之互補股去氧核醣核酸全長序列釣取...........27 2.1.3.2 葉變色相關基因於葉片表現量觀察...........................................30 2.1.4 葉片生長發育相關基因表現量觀察................................................31 2.2 微核醣核酸基因......................................................................................33 2.2.1 微矩陣實驗設計................................................................................33 2.2.2 微核醣核酸基因表現量觀察............................................................34 2.3 葉變色相關基因之基因體去氧核醣核酸序列分析..............................35 2.3.1 啟動子序列及基因體去氧核醣核酸序列釣取................................35 2.3.2 啟動子序列分析................................................................................37 第三章 實驗結果.....................................................................................................38 第一節 生長發育時期之劃分.........................................................................38 1.1 葉片影像檔..............................................................................................38 1.2 葉片色素量測..........................................................................................42 1.3葉片生長發育相關基因及葉變色相關基因表現之即時定量...............45 第二節 微核醣核酸.........................................................................................47 2.1 微矩陣資料分析......................................................................................47 2.2 微核醣核酸參與楓香葉片生長發育調控..............................................49 第三節 葉變色相關基因.................................................................................51 3.1 基因體序列..............................................................................................51 3.1.1 啟動子序列分析.................................................................................51 3.1.2 基因體去氧核醣核酸序列分析.........................................................53 3.2 互補股去氧核醣核酸基因全長序列......................................................54 第四章 討論.............................................................................................................75 第一節 生長發育時期之劃分.........................................................................75 1.1 楓香葉片生長發育色素變化..................................................................75 1.1.1 葉綠素.................................................................................................75 1.1.2 花青素.................................................................................................76 1.2 楓香葉片生長發育基因表現情形..........................................................77 1.3 楓香葉片生長發育之事件時間序列......................................................80 第二節 微核醣核酸.........................................................................................82 2.1 楓香葉片不同生長發育時期之微核醣核酸基因表現情形..................82 2.1.1在綠葉樣本表現量較高者..................................................................82 2.1.2老化葉片中表現量較高者..................................................................83 2.1.3紅葉中表現相對較少者......................................................................84 2.1.4黃葉中表現相對較少者......................................................................85 2.1.5紅葉中表現相對較多者......................................................................86 2.1.6黃葉中表現相對較多者......................................................................86 2.2目標微核醣核酸基因家族參與調控楓香葉片發育................................87 第三節 葉變色相關基因.................................................................................89 3.1 基因體序列..............................................................................................89 3.1.1 啟動子序列分析.................................................................................89 3.1.2 基因體去氧核醣核酸序列分析.........................................................91 3.2 互補股去氧核醣核酸基因全長序列......................................................92 第四節 微核醣核酸參與楓香秋季葉變色之調控.........................................92 第五章 結論.............................................................................................................95 參考文獻.....................................................................................................................96 已發表相關著作.......................................................................................................107 附錄 附件一 中央氣象局氣候報告（節錄）............................................................108 附件二 樣本樹2011年修枝說明......................................................................109 | |
| dc.language.iso | zh-TW | |
| dc.title | 基因與微核醣核酸於楓香秋季葉變色表現之探討 | zh_TW |
| dc.title | Gene and MicroRNA Expression in Autumn Leaf Coloration of
Formosan Sweetgum (Liquidambar formosana Hance) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 簡慶德(Ching-Te Chien),王升陽(Sheng-Yang Wang),林詩舜(Shih-Shan Lin),鹿兒陽(Erh-Yang Lu) | |
| dc.subject.keyword | 微核醣核酸,楓香,秋季葉變色,秋季葉老化,微矩陣, | zh_TW |
| dc.subject.keyword | MicroRNAs,Liquidambar formosana,autumn leaf coloration,autumn leaf senescence,Microarray, | en |
| dc.relation.page | 109 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-08-06 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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