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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 盧虎生(Hu-Sheng Lu) | |
| dc.contributor.author | Yi-Ting Cheng | en |
| dc.contributor.author | 鄭伊婷 | zh_TW |
| dc.date.accessioned | 2021-06-16T17:54:11Z | - |
| dc.date.available | 2015-08-17 | |
| dc.date.copyright | 2012-08-17 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-12 | |
| dc.identifier.citation | 于鳳池、姚堅、姚海根。2009。水稻花藥培養的影響因素及其發展探析。現代農業科技 15: 59-62。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64553 | - |
| dc.description.abstract | 花藥培養產生之單倍體植株經染色體加倍後獲得的純合同質雙單倍體，可廣泛應用於作物品種改良、基因圖譜、基因轉殖、突變遺傳和細胞遺傳等方面的研究。本論文使用臺灣蝴蝶蘭、姬蝴蝶蘭等兩種臺灣原生種蘭花和滿山紅蝴蝶蘭、迷文文心蘭等兩種啇業品種蘭花的花粉塊作為材料進行培養，探討不同因子對花粉塊誘導之影響，並觀察小孢子的發育形態，以嘗試建立起初步的蘭花花藥培養系統。
姬蝴蝶蘭6 mm以上花苞內的花粉塊經50 ppm GA溶液處理一天後，置於蔗糖濃度為6%的培養基內，並於黑暗環境下進行培養時，會具有較高的誘導率。迷你文心蘭的花苞長度為6-7 mm，此即小孢子處於單核期至雙核期階段時最適合用來進行培養，且培養基含糖量為6%時誘導表現會最佳，20%濃度下則無法形成針狀構造。從臺灣蝴蝶蘭的花苞及所有蘭花的盛開花朵中所取出的花粉塊，無論在何種處理下皆無法被誘導。 從外觀上來看，成功被誘導的花粉塊表面會產生許多透明的針狀結構，而顯微鏡下觀察到的小孢子細胞也有向外延伸出長管狀構造的情況出現。部分小孢子的外層物質會遭到瓦解，因此造成小孢子間會相互交疊或彼此游離分散，也有些小孢子的外層物質雖然仍存在，但內含的小孢子數量會多於4個而形成一個形狀不規則的小孢子團。 將產生透明針狀結構的花粉塊移至含有3%蔗糖、1 mg/l NAA、4 mg/l kinetin和0.2%(w/v)活性碳的MS固態培養基中誘導其進行器官分化，培養基內的花粉塊會逐漸白化，且形成的針狀構造會變的粗短、稀疏或完全消失，但沒有任何芽體或不定根的形成。 | zh_TW |
| dc.description.abstract | Homozygous double haploids (DHs) generated from haploid plants through chromosome doubling in anther culture, are widely used in crop breeding programs. It has been useful in such research areas as gene mapping, genetic transformation, mutation studies, and cytogenetic studies. In order to investigate effects of different factors on induction of pollinia and development of microspores, and to establish primary protocols of anther culture of orchids, pollinia from two Taiwan native species orchids Phalaenopsis aphrodite sub. formosana Shimadzu, Phalaenopsis equestris (Schauer) Reichb. f. and two commercial cultivars Tolumnia hybrid, Phalaenopsis spp. hybrid ‘Manshanhung’ were used as materials in this thesis.
The percentages of induction were higher when pollinia from the ca. 6 mm length of buds of P. equestris (Schauer) Reichb. f. were treated with 50 ppm GA solution for one day and cultured on the medium with 6% sucrose in the dark. The results indicated that buds of Tolumnia hybrid about 6-7 mm in length was appropriate for culture, and the microspores at that bud size were at uninucleate to binucleate stage. Besides, the best response was obtained when medium containing 6% sucrose but pollinia can’t differentiate in the 20% sucrose medium. Pollinia from premature buds of Phalaenopsis aphrodite sub. formosana and from open flowers of four orchids did not have any reaction in all treatments. There are a lot of crystalloid needle-like structures on the surface of differentiated pollinia and long tube-like structures could be observed on microspores. Substances that hold microspores together disappeared in some case, so microspores might be on top of another or away from others. Some microspores divided repeatedly and resulted in syncytia of more than 4 nuclei. The color of pollinia turned white and the needle-like structure became shortened or disappeared when the pollinia were subcultured in the differentiation medium on MS salts, 3% sucrose, 1 mg/l NAA, 4 mg/l kinetin, 0.2% (w/v) charcoal, and 0.8% (w/v) agar. Shoots and roots could not be generated in the tested differentiation medium. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T17:54:11Z (GMT). No. of bitstreams: 1 ntu-101-R98621101-1.pdf: 2472962 bytes, checksum: d034300b3ea87b26468010284bf719d1 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 口試委員會審定書…………………………………………Ⅰ
誌謝…………………………………………………………Ⅱ 中文摘要……………………………………………………Ⅲ 英文摘要……………………………………………………Ⅳ 目錄…………………………………………………………Ⅵ 圖目錄………………………………………………………Ⅷ 表目錄………………………………………………………Ⅸ 前言…………………………………………………………1 前人研究……………………………………………………3 一、單倍體植物發展簡史…………………………………3 二、單倍體植物的離體誘導方法…………………………3 三、小孢子發育模式………………………………………5 四、離體培養下單倍體植株的形成方式…………………7 五、影響花藥培養效率的因子……………………………10 材料與方法…………………………………………………24 一、實驗材料………………………………………………24 二、實驗方法………………………………………………24 (一)第一次誘導試驗………………………………………25 (二)植物激素處理…………………………………………27 (三)第二次誘導試驗………………………………………27 (四)植物激素處理…………………………………………28 (五)第三次誘導試驗………………………………………28 (六)迷你文心蘭小孢子發育時期鑑定……………………29 (七)第四次誘導試驗………………………………………29 (八)分化誘導………………………………………………30 結果…………………………………………………………33 一、第一次誘導試驗………………………………………33 二、植物激素處理…………………………………………33 三、第二次誘導試驗………………………………………34 四、植物激素處理…………………………………………34 五、第三次誘導試驗………………………………………34 六、迷你文心蘭小孢子發育時期鑑定……………………36 七、第四次誘導試驗………………………………………36 八、分化誘導………………………………………………37 討論…………………………………………………………38 一、小孢子發育時期………………………………………38 二、植物生長調節劑………………………………………39 三、蔗糖濃度………………………………………………41 四、基本培養基、預冷處理與照明環境…………………42 五、花粉與小孢子的發育型態……………………………43 六、分化誘導………………………………………………44 七、未來研究方向…………………………………………45 參考文獻……………………………………………………47 圖目錄 圖1. 一般被子植物的小孢子發育途徑………………………5 圖2. 菸草小孢子於離體培養下直接發育之途徑……………8 圖3. 小孢子於胚發生早期所進行的各種細胞核分裂途徑…10 圖4. 小孢子兩種發育途徑之圖示……………………………14 圖5. 實驗進行流程圖…………………………………………24 圖6. 姬蝴蝶蘭花粉塊於1/2 MS培養基中黑暗誘導下之發育情形…63 圖7. 臺灣蝴蝶蘭花粉塊的小孢子發育時期…………………64 圖8. 姬蝴蝶蘭花粉塊的小孢子發育時期……………………65 圖9. 姬蝴蝶蘭花粉塊於培養下之形態變化…………………66 圖10. 姬蝴蝶蘭具針狀構造之花粉塊其小孢子發育情形……67 圖11. 迷你文心蘭花粉塊於培養下之形態變化.……………68 圖12. 迷你文心蘭具針狀構造之花粉塊其小孢子發育情形…69 圖13. 於分化培養基內進行誘導之花粉塊……………………70 表目錄 表1. 蘭科植物成熟花粉之型態………………………………6 表2. 臺灣蝴蝶蘭花苞長度與其小孢子發育時期之關係……30 表3. MS培養基與B5培養基之組成份…………………………31 表4. 姬蝴蝶蘭花苞長度與其小孢子發育時期之關係………32 表5-1. 姬蝴蝶蘭花粉塊培養結果(第三次誘導試驗)………56 表5-2. 姬蝴蝶蘭花粉塊培養結果(第三次誘導試驗)………57 表5-3. 姬蝴蝶蘭花粉塊培養結果(第三次誘導試驗)………58 表6. 迷你文心蘭花苞長度與其小孢子發育時期之關係……59 表7-1. 迷你文心蘭花粉塊培養結果(第四次誘導試驗)……60 表7-2. 迷你文心蘭花粉塊培養結果(第四次誘導試驗)………61 表7-3. 迷你文心蘭花粉塊培養結果(第四次誘導試驗)………62 | |
| dc.language.iso | zh-TW | |
| dc.subject | 蘭花 | zh_TW |
| dc.subject | 花藥培養 | zh_TW |
| dc.subject | 小孢子發育 | zh_TW |
| dc.subject | 植物激素 | zh_TW |
| dc.subject | 蔗糖濃度 | zh_TW |
| dc.subject | Sucrose concentration | en |
| dc.subject | Orchids | en |
| dc.subject | Microspore development | en |
| dc.subject | Anther culture | en |
| dc.subject | Plant hormones | en |
| dc.title | 蘭花花粉塊組織培養下小孢子發育的研究 | zh_TW |
| dc.title | Studies on the development of orchid microspores in vitro culture of pollinium | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 張松彬(Song-Bin Chang) | |
| dc.contributor.oralexamcommittee | 陳文輝(Wen-Huei Chen) | |
| dc.subject.keyword | 蘭花,花藥培養,小孢子發育,植物激素,蔗糖濃度, | zh_TW |
| dc.subject.keyword | Orchids,Anther culture,Microspore development,Plant hormones,Sucrose concentration, | en |
| dc.relation.page | 70 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-08-13 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| 顯示於系所單位： | 農藝學系 | |
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