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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 沈偉強(Wei-Chiang Shen) | |
dc.contributor.author | Yu-Lun Liu | en |
dc.contributor.author | 劉育倫 | zh_TW |
dc.date.accessioned | 2021-06-16T17:48:51Z | - |
dc.date.available | 2017-08-28 | |
dc.date.copyright | 2012-08-28 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-13 | |
dc.identifier.citation | Blee, E. 1998. Phytooxylipins and plant defense reactions. Prog. Lipid Res. 37:33-72.
Boter, M., Ruiz-Rivero, O., Abdeen, A., and Prat, S. 2004. Conserved MYC transcription factors play a key role in jasmonate signaling both in tomato and Arabidopsis. Genes Dev. 18:1577-1591. Browse, J. 2009. Jasmonate passes muster: a receptor and targets for the defense hormone. Annu. Rev. Plant Biol. 60:183-205. Chini, A., Boter, M., and Solano, R. 2009a. Plant oxylipins: COI1/JAZs/MYC2 as the core jasmonic acid-signalling module. FEBS J. 276:4682-4692. Chini, A., Fonseca, S., Chico, J.M., Fernandez-Calvo, P., and Solano, R. 2009b. The ZIM domain mediates homo- and heteromeric interactions between Arabidopsis JAZ proteins. Plant J. 59:77-87. Chini, A., Fonseca, S., Fernandez, G., Adie, B., Chico, J.M., Lorenzo, O., Garcia-Casado, G., Lopez-Vidriero, I., Lozano, F.M., Ponce, M.R., Micol, J.L., and Solano, R. 2007. The JAZ family of repressors is the missing link in jasmonate signalling. Nature 448:666-671. Chung, H.S., and Howe, G.A. 2009. A critical role for the TIFY motif in repression of jasmonate signaling by a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10 in Arabidopsis. Plant cell 21:131-145. Chung, H.S., Koo, A.J.K., Gao, X., Jayanty, S., Thines, B., Jones, A.D., and Howe, G.A. 2008. Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in response to wounding and herbivory. Plant Physiol. 146:952-964. Chung, H.S., Cooke, T.F., DePew, C.L., Patel, L.C., Ogawa, N., Kobayashi, Y., and Howe, G.A. 2010. Alternative splicing expands the repertoire of dominant JAZ repressors of jasmonate signaling. Plant J. 63:613-622. Conconi, A., Smerdon, M.J., Howe, G.A., and Ryan, C.A. 1996. The octadecanoid signalling pathway in plants mediates a response to ultraviolet radiation. Nature 383:826-829. Creelman, R.A., and Mullet, J.E. 1997. Biosynthesis and action of jasmonates in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:355-381. del Pozo, J.C., and Estelle, M. 2000. F-box proteins and protein degradation: an emerging theme in cellular regulation. Plant Mol. Biol. 44:123-128. Devoto, A., Nieto-Rostro, M., Xie, D., Ellis, C., Harmston, R., Patrick, E., Davis, J., Sherratt, L., Coleman, M., and Turner, J.G. 2002. COI1 links jasmonate signalling and fertility to the SCF ubiquitin–ligase complex in Arabidopsis. Plant J. 32:457-466. Dombrecht, B., Xue, G.P., Sprague, S.J., Kirkegaard, J.A., Ross, J.J., Reid, J.B., Fitt, G.P., Sewelam, N., Schenk, P.M., Manners, J.M., and Kazan, K. 2007. MYC2 differentially modulates diverse jasmonate-dependent functions in Arabidopsis. Plant cell 19:2225-2245. Farmer, E.E., and Ryan, C.A. 1990. Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proceedings of the National Academy of Sciences 87:7713-7716. Fernandez-Calvo, P., Chini, A., Fernandez-Barbero, G., Chico, J.-M., Gimenez-Ibanez, S., Geerinck, J., Eeckhout, D., Schweizer, F., Godoy, M., Franco-Zorrilla, J.M., Pauwels, L., Witters, E., Puga, M.I., Paz-Ares, J., Goossens, A., Reymond, P., De Jaeger, G., and Solano, R. 2011. The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses. Plant cell 23:701-715. Feys, B.J.F., Benedetti, C.E., Penfold, C.N., and Turner, J.G. 1994. Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogen. Plant cell 6:751-759. Fonseca, S., Chico, J.M., and Solano, R. 2009. The jasmonate pathway: the ligand, the receptor and the core signalling module. Curr. Opin. Plant Biol. 12:539-547. Glazebrook, J. 2005. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu. Rev. Phytopathol. 43:205-227. He, Y., Fukushige, H., Hildebrand, D.F., and Gan, S. 2002. Evidence supporting a role of jasmonic acid in Arabidopsis leaf senescence. Plant Physiol. 128:876-884. Howe, G.A., and Jander, G. 2008. Plant immunity to insect herbivores. Annu. Rev. Plant Biol. 59:41-66. Katsir, L., Chung, H.S., Koo, A.J.K., and Howe, G.A. 2008. Jasmonate signaling: a conserved mechanism of hormone sensing. Curr. Opin. Plant Biol. 11:428-435. Kazan, K., and Manners, J.M. 2008. Jasmonate signaling: toward an integrated view. Plant Physiol. 146:1459-1468. Kloek, A.P., Verbsky, M.L., Sharma, S.B., Schoelz, J.E., Vogel, J., Klessig, D.F., and Kunkel, B.N. 2001. Resistance to Pseudomonas syringae conferred by an Arabidopsis thaliana coronatine-insensitive (coi1) mutation occurs through two distinct mechanisms. Plant J. 26:509-522. Koo, A.J.K., and Howe, G.A. 2009. The wound hormone jasmonate. Phytochemistry 70:1571-1580. Koo, A.J.K., Gao, X., Daniel Jones, A., and Howe, G.A. 2009. A rapid wound signal activates the systemic synthesis of bioactive jasmonates in Arabidopsis. Plant J. 59:974-986. Li, J.F., Park, E., von Arnim, A., and Nebenfuhr, A. 2009. The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species. Plant Methods 5:6. Li, L., Zhao, Y., McCaig, B.C., Wingerd, B.A., Wang, J., Whalon, M.E., Pichersky, E., and Howe, G.A. 2004. The tomato homolog of CORONATINE-INSENSITIVE1 is required for the maternal control of seed maturation, jasmonate-signaled defense responses, and glandular trichome development. Plant cell 16:126-143. Liu, Y., Schiff, M., and Dinesh-Kumar, S.P. 2004. Involvement of MEK1 MAPKK, NTF6 MAPK, WRKY/MYB transcription factors, COI1 and CTR1 in N-mediated resistance to tobacco mosaic virus. Plant J. 38:800-809. Lorenzo, O., and Solano, R. 2005. Molecular players regulating the jasmonate signalling network. Curr. Opin. Plant Biol. 8:532-540. Lorenzo, O., Chico, J.M., Sanchez-Serrano, J.J., and Solano, R. 2004. JASMONATE-INSENSITIVE1 encodes a MYC transcription factor essential to discriminate between different jasmonate-regulated defense responses in Arabidopsis. Plant cell 16:1938-1950. Lu, H.C., Hsieh, M.H., Chen, C.E., Chen, H.H., Wang, H.I., and Yeh, H.H. 2012. A high-throughput virus-induced gene-silencing vector for screening transcription factors in virus-induced plant defense response in orchid. Mol. Plant-Microbe Interact. 25:738-746. Lu, H.C., Chen, H.H., Tsai, W.C., Chen, W.H., Su, H.J., Chang, D.C.N., and Yeh, H.H. 2007. Strategies for functional validation of genes involved in reproductive stages of orchids. Plant Physiol. 143:558-569. Mach, J. 2009. The jasmonate receptor: protein modeling and photoaffinity labeling reveal that the CORONATINE INSENSITIVE1 protein binds jasmonoyl-isoleucine and coronatine. Plant cell 21:2192. Memelink, J. 2009. Regulation of gene expression by jasmonate hormones. Phytochemistry 70:1560-1570. Moon, J., Parry, G., and Estelle, M. 2004. The ubiquitin-proteasome pathway and plant development. Plant cell 16:3181-3195. Niu, Y., Figueroa, P., and Browse, J. 2011. Characterization of JAZ-interacting bHLH transcription factors that regulate jasmonate responses in Arabidopsis. J. Exp. Bot. 62:2143-2154. Paschold, A., Halitschke, R., and Baldwin, I.T. 2007. Co(i)-ordinating defenses: NaCOI1 mediates herbivore- induced resistance in Nicotiana attenuata and reveals the role of herbivore movement in avoiding defenses. Plant J. 51:79-91. Peng, S.Q., Xu, J., Li, H.L., and Tian, W.M. 2009. Cloning and molecular characterization of HbCOI1 from Hevea brasiliensis. Biosci., Biotechnol., Biochem. 73:665-670. Pruss, G.J., Nester, E.W., and Vance, V. 2008. Infiltration with Agrobacterium tumefaciens induces host defense and development-dependent responses in the infiltrated zone. Mol. Plant-Microbe Interact. 21:1528-1538. Reinbothe, C., Springer, A., Samol, I., and Reinbothe, S. 2009. Plant oxylipins: role of jasmonic acid during programmed cell death, defence and leaf senescence. FEBS J. 276:4666-4681. Rico, A., Bennett, M.H., Forcat, S., Huang, W.E., and Preston, G.M. 2010. Agroinfiltration reduces ABA levels and suppresses Pseudomonas syringae-elicited salicylic acid production in Nicotiana tabacum. PLoS ONE 5:e8977. Schaller, A., and Stintzi, A. 2009. Enzymes in jasmonate biosynthesis – structure, function, regulation. Phytochemistry 70:1532-1538. Schaller, F., Biesgen, C., Mussig, C., Altmann, T., and Weiler, E.W. 2000. 12-Oxophytodienoate reductase 3 (OPR3) is the isoenzyme involved in jasmonate biosynthesis. Planta 210:979-984. Sheard, L.B., Tan, X., Mao, H., Withers, J., Ben-Nissan, G., Hinds, T.R., Kobayashi, Y., Hsu, F.-F., Sharon, M., Browse, J., He, S.Y., Rizo, J., Howe, G.A., and Zheng, N. 2010. Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Nature 468:400-405. Staswick, P.E. 2008. JAZing up jasmonate signaling. Trends Plant Sci. 13:66-71. Thatcher, L.F., Manners, J.M., and Kazan, K. 2009. Fusarium oxysporum hijacks COI1-mediated jasmonate signaling to promote disease development in Arabidopsis. Plant J. 58:927-939. Thines, B., Katsir, L., Melotto, M., Niu, Y., Mandaokar, A., Liu, G., Nomura, K., He, S.Y., Howe, G.A., and Browse, J. 2007. JAZ repressor proteins are targets of the SCFCOI1 complex during jasmonate signalling. Nature 448:661-665. Thomma, B.P.H.J., Eggermont, K., Penninckx, I.A.M.A., Mauch-Mani, B., Vogelsang, R., Cammue, B.P.A., and Broekaert, W.F. 1998. Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proceedings of the National Academy of Sciences 95:15107-15111. Tsai, W.C., Hsiao, Y.Y., Lee, S.H., Tung, C.W., Wang, D.P., Wang, H.C., Chen, W.H., and Chen, H.H. 2006. Expression analysis of the ESTs derived from the flower buds of Phalaenopsis equestris. Plant Sci. 170:426-432. Turner, J.G., Ellis, C., and Devoto, A. 2002. The jasmonate signal pathway. Plant cell 14:S153-S164. Van der Ent, S., Van Wees, S.C.M., and Pieterse, C.M.J. 2009. Jasmonate signaling in plant interactions with resistance-inducing beneficial microbes. Phytochemistry 70:1581-1588. Wang, Z., Dai, L., Jiang, Z., Peng, W., Zhang, L., Wang, G., and Xie, D. 2005. GmCOI1, a soybean F-Box protein gene, shows ability to mediate jasmonate-regulated plant defense and fertility in Arabidopsis. Mol. Plant-Microbe Interact. 18:1285-1295. Xie, D.X., Feys, B.F., James, S., Nieto-Rostro, M., and Turner, J.G. 1998. COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility. Science 280:1091-1094. Xu, L., Liu, F., Lechner, E., Genschik, P., Crosby, W.L., Ma, H., Peng, W., Huang, D., and Xie, D. 2002. The SCF(COI1) ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis. Plant cell 14:1919-1935. Yan, J., Zhang, C., Gu, M., Bai, Z., Zhang, W., Qi, T., Cheng, Z., Peng, W., Luo, H., Nan, F., Wang, Z., and Xie, D. 2009. The Arabidopsis CORONATINE INSENSITIVE1 protein is a jasmonate receptor. Plant cell 21:2220-2236. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64466 | - |
dc.description.abstract | 蝴蝶蘭屬(Phalaenopsis)蘭科植物為台灣外銷產值最高的花卉作物,但其於栽培過程中可能受到病原菌之感染危害,造成生產者的重大損失。Coronatine-insensitive 1 gene (COI1)為jasmonic acid (JA)路徑重要的基因,主要功能為感受細胞中JA濃度,並調控JA下游基因的表現,如抗病性基因、老化基因、根部發育、雄性花器成熟等。本實驗利用阿拉伯芥COI1基因核酸序列,進行蝴蝶蘭Expressed sequence tag (EST) library之比對,得到256 bp長度之序列片段,進一步利用Rapid Amplification of Complementary DNA Ends (RACE) 方法,得到蝴蝶蘭PaCOI1基因之cDNA全長,預測轉譯出585個胺基酸之蛋白質。親緣關係比對結果顯示,蝴蝶蘭PaCOI1 胺基酸序列與已發表的其他物種中,較親近於同為單子葉植物的小麥及水稻同源基因。在基因表現方面,蝴蝶蘭PaCOI1基因於根、莖、葉、花梗、花等部位,可偵測到其表現;在傷口處理與MeJA施用的情形下,PaCOI1所調控之PaJAZ1與PaMYC2基因可於2小時內,快速提高其表現量,而因傷口所引發PaJAZ1 和PaMYC2 大量mRNA累積之現象,與PaCOI1基因相關。而因應不同病原菌的感染,PaJAZ1與PaMYC2基因表現上,亦有所差異。為了解PaCOI1基因之功能,分別建構靜默(silencing)與過度表現(overexpression)載體,並應用Agrobacterium-mediated transient gene expression and silencing工具,進行蝴蝶蘭中PaCOI1基因表現調控影響之觀察。VIGS 靜默植株PaCOI1表現量約可降低至3到4成,接種黃葉病菌於PaCOI1基因靜默植株,呈現感病加劇之情形。黃葉病菌與軟腐病菌於PaCOI1過度表現植株中,病斑發展並無顯著差異。利用 Fast Agro-mediated Seedling Transformation (FAST) 方法,已成功於阿拉伯芥中表現PaCOI1基因。本研究結果證實,蝴蝶蘭PaCOI1基因為病原菌感染及傷口所引起JA反應路徑之重要基因。 | zh_TW |
dc.description.abstract | Phalaenopsis belongs to Orchidaceae, and is one of the most valuable flower crops for sale abroad in Taiwan. In orchid nursery, Phalaenopsis is subjected to infection by many pathogens during different growing periods, and severe economic losses to growers often occur. Coronatine-insensitive 1 gene (COI1) is responsible of sensing jasmonic acid (JA) inside cells, and regulates downstream JA-responsive genes involved in disease resistance, senescence, root development, and male fertility. In this study, we utilized Arabidopsis COI1 gene sequence to search against Phalaenopsis EST library, and identified a partial 256 bp fragment of Phalaenopsis COI1 homologue. By Rapid Amplification of Complementary DNA Ends (RACE) methods, the full length of Phalaenopsis aphrodite COI1 (PaCOI1) cDNA was obtained and found PaCOI1 encodes a 585 amino acid residues protein. Phylogenetic analysis revealed that PaCOI1 is more closely related to homologues of monocot plants such as Oryza sativa and Triticum aestivum. The basal PaCOI1 transcript levels can be detected in root, stem, leaf, stalk and flower. After wounding and exogenous MeJA treatments, PaJAZ1 and PaMYC2 genes, two downstream targets in JA signaling pathway, were quickly and dramatically induced within two hours. Wound-induced accumulation of e PaJAZ1 and PaMYC2 mRNAs was largely dependent on PaCOI1. In response to pathogen infection, PaJAZ1 and PaMYC2 were also differentially regulated. In order to determine the function of PaCOI1 gene, silencing and overexpression vectors were constructed and Agrobacterium-mediated transient gene expression and silencing systems were used. Gene expression studies confirmed that PaCOI1 in VIGS silencing plants was reduced to 30-40% of the wild-type level. When challenging silencing plants with different pathogens, more severe symptoms were found by F. solani infection. No significant difference in lesion development of PaCOI1 overexpression plants was found when infected with F. solani or E. chrysanthemi. Finally, Fast Agro-mediated Seedling Transformation (FAST) method was successfully employed to deliver and express PaCOI1 in Arabidopsis. Our studies demonstrate that PaCOI1 is an important P. aphrodite gene involved in pathogen and wounding induced JA signaling pathway. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T17:48:51Z (GMT). No. of bitstreams: 1 ntu-101-R98633002-1.pdf: 2019711 bytes, checksum: b1067642f31a23a0e1c104e0d2f3594d (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii CONTENTS v LIST OF FIGURES ix LIST OF TABLES x Chapter 1 Introduction 1 1.1 The important roles of jasmonate in plant physiology 1 1.2 The biosynthesis and regulation of jasmonic acid 2 1.3 The jasmonate signal sensing and its roles in defense responses 3 1.4 Molecular cloning of COI1 gene in Arabidopsis 5 1.5 The role of SCFCOI1 complex and its role in jasmonate pathway in Arabidopsis 6 1.6 The Arabidopsis coi1 mutant phenotype in plant defense 7 1.7 Molecular cloning of COI1 gene in other plant species 8 1.8 Other important proteins in JA pathway 11 1.8.1 JAZ repressor proteins are targets of the SCFCOI1 complex and participate in JA pathway 11 1.8.2 MYC2 transcription factor, a bHLH-leu zipper protein, is essential for different jasmonate regulated defense responses 12 Chapter 2 Materials and Methods 14 2.1 Plant materials 14 2.2 Cloning of Phalaenopsis COI1 gene 14 2.2.1 EST library search 14 2.2.2 RNA extraction method 15 2.2.3 DNase treatment 15 2.2.4 Full length of Phalaenopsis COI1 cDNA with 5' and 3' RACE methods 16 2.2.5 BAC library hybridization 17 2.2.6 Determine the Phalaenopsis COI1 gene intron positions 17 2.3 Alignment of the COI1 homologues and generation of the phylogentic tree 18 2.4 Southern blot hybridization 19 2.4.1 Genomic DNA extraction 19 2.4.2 Enzyme digestion of genomic DNA 19 2.4.3 Capillary transfer 20 2.4.4 Preparation of DIG and radioactive-labeled probes 20 2.4.5 Hybridization and detection 21 2.5 Gene expression analyses of PaCOI1 and JA related genes 22 2.5.1 RT-PCR 22 2.5.2 Real-time RT-PCR 23 2.6 Agrobacterium based transient gene expression construct 23 2.6.1 Construction of PaCOI1 VIGS silencing vector 23 2.6.2 Construction of COI1 gene overexpression vector 24 2.7 Culture conditions and Agrobacterium infiltration 24 2.7.1 Electroporation 24 2.7.2 Agroinfiltration 25 2.8 Wounding treatment and exogenous MeJA application 25 2.8.1 Wounding treatment 25 2.8.2 Exogenous MeJA application 25 2.9 Inoculation of E. chrysanthemi and F. solani 26 2.9.1 Preparation of E. chrysanthemi inoculate 26 2.9.2 Preparation of F. solani inoculate 26 2.9.3 Inoculation of E. chrysanthemi and F.solani 26 2.10 The FAST method for transient gene expression in Arabidopsis 27 2.10.1 Agrobacterium growth 27 2.10.2 Fast agro-mediated seeding transformation 27 2.11 Root growth inhibition assay 28 Chapter 3 Results 29 3.1 Cloning and characterization of Phalaenopsis aphrodite COI1 (PaCOI1) gene 29 3.1.1 Identification of Phalaenopsis aphrodite COI1 (PaCOI1) gene in Phalaenopsis EST library 29 3.1.2 One copy of COI1 gene is present in the Phalaenopsis genome 29 3.1.3 Identification of PaCOI1 clone in Phalaenopsis BAC library 30 3.1.4 Cloning of PaCOI1 full-length cDNA 30 3.1.5 Two introns exist in PaCOI1 gene 31 3.1.6 The promoter region of PaCOI1 gene 32 3.2 PaCOI1 contains a conserved F-box motif and shows highest similarity to the COI1 homologues of monocot plants 32 3.3 The expression patterns of PaCOI1 gene in different organs and under different conditions 33 3.3.1 PaCOI1 is differentially expressed in different organs of P. aphrodite 33 3.3.2 PaCOI1 and JA-related genes were induced in response to wounding and MeJA treatment. 34 3.3.3 PaCOI1 and JA-related genes expression level after inoculation of E. chrysanthemi and F. solani. 36 3.4 Functional analysis of PaCOI1 gene 37 3.4.1 The expressions of PaCOI1 and JA-related gene in PaCOI1 silenced plants were reduced or delayed in response to wounding treatment 37 3.4.2 The phenotypes of PaCOI1 silenced plants when challenging with E. chrysanthemi and F. solani 38 3.4.3 The phenotype of F. solani inoculated on VIGS_COI1 silencing plants and JA-related genes expression pattern. 39 3.4.4 The phenotype of F. solani and E. chrysanthemi inoculated on overexpression_COI1 plants 39 3.5 Arabidopsis coi1 mutant phenotype and transient expression of -PaCOI1 gene in Arabidopsis 40 3.5.1 Arabidopsis coi1 mutants show insensitive to MeJA by root growth inhibition assay 40 3.5.2 Transient overexpression of PaCOI1 gene in WT and coi1 mutant plants by FAST method 40 Chapter 4 Discussions 42 Figures and tables 47 REFERENCE 80 Appendix 87 | |
dc.language.iso | en | |
dc.title | 蝴蝶蘭PaCOI1基因之選殖及參與茉莉酸訊息傳導及抗病性研究 | zh_TW |
dc.title | Molecular cloning and characterization of Phalaenopsis aphrodite PaCOI1 gene in JA pathway and plant disease resistance | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳虹樺(Hong-Hwa Chen),葉信宏(Hsin-Hung Yeh) | |
dc.subject.keyword | 蝴蝶蘭,jasmonic acid (JA),Coronatine-insensitive 1 gene (COI1),Virus induced gene silencing (VIGS),Fast agro-mediated seedling transformation (FAST), | zh_TW |
dc.subject.keyword | Phalaenopsis aphrodite,jasmonic acid (JA),Coronatine-insensitive 1 gene (COI1),Virus induced gene silencing (VIGS),Fast agro-mediated seedling transformation (FAST), | en |
dc.relation.page | 88 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-08-14 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
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