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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 沈弘俊(Horn-Jiunn Sheen) | |
dc.contributor.author | Jen-Chieh Lu | en |
dc.contributor.author | 呂仁傑 | zh_TW |
dc.date.accessioned | 2021-06-16T17:45:47Z | - |
dc.date.available | 2019-12-19 | |
dc.date.copyright | 2012-08-22 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-14 | |
dc.identifier.citation | Angele, K. P., Suzuki , Miwa, Y., J., Kasagi, N., Yamaguchi Y., “Development of a high-speed Scanning micro-PIV system”6th International Symposium on Particle Image Velocimetry Pasadena (2005)
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Lee, “Immunoassays in Nanoliter Volume Reactors Using Fluorescent Particle Diffusometry” Langmuir, vol. 24, pp. 2947-2952 (2008) Yahng, J.S., Jeoung, S.C., Choi, D.S., Kim, J.H., Choi, H.M., Paik, J. S., “Fabrication of microfluidic devices by using a femtosecond laser micromachining technique and μ-PIV studies on its fluid dynamics” Journal of the Korean Physical Society, 47 N.O. 6 pp.977-981 (2005) Yasuhiko, Sugii, Okuda, Remi, Okamoto, Koji, Madarame, Haruki, ”Velocity measurement of both red blood cells and plasma of in vitro blood flow using high-speed micro PIV technique” Measurement Science and Technology, vol. 16, pp.1126-1130(2005) 范育睿,“利用微粒子追蹤測速儀量測C反應蛋白之布朗運動及其反應檢測”,國立台灣大學應用力學研究所碩士論文 (2007) 鄧喬廸,“量測奈米粒子之布朗運動用於檢測齒舌蘭輪斑病毒”,國立台灣大學應用力學研究所碩士論文 (2011) 顏毅廣,“全反射螢光顯微技術應用於蛋白質分子之即時偵測與操控”,國立台灣大學應用力學研究所碩士論文 (2004) 李育嘉,“漫談布朗運動”,數學傳播第廿八卷第一期 (2006) 黃文璋,“布朗運動簡介”,數學傳播第十六卷第四期 (1992) 龐寧寧,“漫談布朗運動”,物理雙月刊第廿八卷第一期 (2006) 王子瑜、曹恒光,“布朗運動、郎之萬方程式、與布朗動力學”,物理雙月刊第廿七卷第三期,pp. 456-460 (2005) | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64414 | - |
dc.description.abstract | 布朗運動檢測技術為一種新式仍在開發中的微型化生物感測元件,具有操作簡易不需利用微機電製程以及易整合於微流道元件中的優點。本研究針對微流道中的布朗運動現象作完整探討,包括濃度變化對布朗運動的影響、布朗運動受壁面的影響、粒徑大小關係以及表面改質後對布朗運動的影響,希望對開發中的感測技術進行最佳化設計。研究方法是使用與抗體抗原相同大小的奈米粒子來做為模擬抗體,實驗方法主要使用微粒子追蹤測速儀 (PTV)針對這些奈米粒子進行速度分析,從速度分佈圖中可看出,布朗運動本身會呈現高斯分佈,最終統計出布朗運動速度,其次部分實驗則使用到螢光相關光譜 (FCS)來進行研究分析,利用針孔(pinhole)來進行空間濾波提升觀測解析度並記錄光強度的變化,最終利用自相關函數來分析出量測樣本的擴散係數。
從實驗中可以看出粒子靠近邊界時受到邊界的影響,布朗運動速度會下降,粒徑增加會使布朗運動速度減慢,同時將邊界進行表面改質之後,由於滑移長度過小,與未經表面改質的對布朗運動速度並無顯著的差異,而當樣本中的粒子濃度提高後,微觀來看使得能量消散更多,巨觀來看就是等效黏滯力提升,造成布朗運動速度下降。未來在智慧型手機發達後應用手機本身的相機當作CCD,分析程式寫成應用程式(APP)希望將此技術應用於日常生活中,落實智慧生活科技。 | zh_TW |
dc.description.abstract | A quantitative immunosensing technique based on the measurement of nanoparticles’ Brownian motion is one of newly innovative bio-sensor chip on miniaturized devices and still in developing. There are some advantages of the technique such as highly compatible to any lab-on-chip device and easily fabricated without micro-electro-mechanical-system (MEMS) process. However, there are still some physical phenomena needed to research in order to optimize this technique. Boundary effect can no more be neglected in a micro system, therefore; this study aims to investigate Brownian motion behaviors in the micro channel, which combines effects of high concentrated nanoparticles, surface modification, different size of nanoparticles and very closed to boundary.
The sizes of particles are chosen by simulating real virus and anti-virus. The measurements and analysis of Brownian motion are primarily set up by using micro Particle-Tracking-Velocimetry (μ-PTV) and secondly set up by Fluorescent Correlation Spectroscopy (FCS). By the velocity profiles, it could be easily found out that Brownian motion present as Gaussian distribution; still, Brownian velocity can be obtained by calculating the standard deviation of particles’ velocity. Another secondly method of measurement Brownian motion is FCS which records the intensity signal of particles and analyzes by autocorrelation function which measures the self-similarity of a time signal and obtains the diffusion coefficient. Whatever analyzed methods be used, the results present that Brownian motion gets slow when the particles close to the boundary due to the boundary effect and no slip condition. The radius of particles is proportional to inversely square Brownian velocity. As concentration of solution gets larger, the effective viscosity of solution gets larger, which makes the Brownian motion becomes slower. Surface modification makes the surface become hydrophobic, and Brownian motion is measured comparing with hydrophilic but the there is no apparently different Brownian velocity between two surfaces. Finally, as smart phone with high pixels camera become popular, analysis program could be written as application, therefore; this innovative technique can be bring into the daily life. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T17:45:47Z (GMT). No. of bitstreams: 1 ntu-101-R99543029-1.pdf: 2815475 bytes, checksum: 52b4a781154a5813ddbb5d70c2fcd6f4 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 中文摘要 ii ABSTRACT iii 目錄 v 表目錄 viii 圖目錄 ix 第一章 緒論 1 1-1 前言 1 1-2 研究動機及目的 2 1-3 文獻回顧 3 1-3.1 布朗運動發展及文獻回顧 3 1-3.2 布朗運動以及邊界效應 5 1-4 研究方法 6 1-5 論文架構 7 第二章 布朗運動 8 2-1 布朗運動數學模型 8 2-2 布朗運動與邊界效應影響 12 2-3 膠體粒子的布朗運動以及沉降平衡 14 第三章 實驗相關原理 17 3-1 微粒子影像與微粒子追蹤測速儀(μPIV/μPTV) 17 3-2 共焦顯微鏡(confocal microscopy) 18 3-3 螢光相關光譜(Fluorescence correlation spectroscopy) 20 3-3.1 螢光相關光譜原理 20 第四章 實驗設備架構與實驗步驟 24 4-1 PTV實驗設備 24 4-1.1 光源裝置 24 4-1.2 同步器(Synchronizer) 24 4-1.3 影像擷取裝置 25 4-1.4 實驗光路配置 25 4-2 FCS 實驗架設 25 4-2.1 實驗光路配置 25 4-2.2 針孔(pinhole)原理以及製作 26 4-3 微流道製作 26 4-3.1 SU-8 母模製作 27 4-4 奈米粒子 28 4-5 視野校正 29 4-5.1 PTV系統視野校正 29 4-5.2 FCS系統視野校正 30 4-6 表面改質 30 第五章 實驗結果與討論 32 5-1 布朗運動研究之分析 32 5-1.1 利用μ-PTV 做布朗運動研究之分析 32 5-1.2 利用FCS 輔助做布朗運動研究之分析 34 5-2 布朗運動與濃度變化之研究 35 5-3 布朗運動與粒徑改變之研究 37 5-4 布朗運動與邊界效應之研究 37 5-5 布朗運動與表面改質之研究 39 第六章 結論與未來展望 41 6-1 結論 41 6-2 未來展望 42 REFERENCES 43 表目錄 表1- 1 各式感測器比較 49 表2- 1 膠體粒子的分類 49 表3- 1 螢光粒子資訊 50 表4- 1 視野校正計算結果 50 圖目錄 圖 1- 1 C-反應蛋白與高密度脂蛋白之相對危險比例 51 圖 1- 2 各式感測器,檢測反應之示意圖 52 圖 2- 1 靠近邊界時,擴散係數修正式 (Brenner et al., 1961) 53 圖 2- 2 膠體粒子沉降平衡以及水深示意圖。 53 圖 3- 1 PTV系統分析流程圖 54 圖 3- 2 PTV系統擷取到的圖 55 圖 3- 3 PTV系統辨認出粒子 55 圖 3- 4 PTV系統分析示意圖 56 圖 3- 5 PTV在比對完兩張影像後,分析判斷對速度及流場 56 圖 3- 6 經由Insight 3G軟體分析出來後的粒子速度分佈圖 57 圖 3- 7 共焦顯微鏡原理 57 圖 3- 8 FCS有效觀測體積示意圖 58 圖 3- 9 自相關函數圖形分佈意義 58 圖 4- 1 micro PTV/PIV 示意圖 59 圖 4- 2 汞燈 60 圖 4- 3 同步器 60 圖 4- 4 倒立式顯微鏡 60 圖 4- 5 PTV拍攝CCD 60 圖 4- 6 FCS光路平面配置圖 61 圖 4- 7 FCS光路配置圖 61 圖 4- 8 波長450nm 雷射 62 圖 4- 9 拍攝用CCD 62 圖 4- 10 使用鎢針刺出的針孔(pinhole)大小為100 um 62 圖 4- 11 SU8母模製作過程 63 圖 4- 12 PDMS 翻模過程 63 圖 4- 13 (a)~(f)分別為六種鏡頭倍率下的視野校正影像(Fan, 2008) 64 圖 4- 14 固、液、氣三相力平衡圖其中 即為接觸角 65 圖 4- 15 接觸角與滑移長度關係圖 (Li et al., 2005) 65 圖 4- 16 正辛基三乙氧基矽烷(n-Octyltriethoxysilane)及其化學式 66 圖 4- 17 矽烷與玻璃材質發生反應示意圖 66 圖 4- 18 蓋玻片未經表面改質接觸角為 67 圖 4- 19 蓋波片經由矽烷表面改質後,接觸角為 67 圖 5- 1 x軸方向布朗運動速度(U m/s)分佈,會呈現高斯分佈 68 圖 5- 2 布朗運動x軸方向速度(U m/s)與位置分佈圖 68 圖 5- 3 y軸方向布朗運動速度(V m/s)分佈,會呈現高斯分佈 69 圖 5- 4 布朗運動y軸方向速度(V m/s)與位置分佈圖 69 圖 5- 5 粒徑大小與x方向速度分佈圖(呈現高斯分佈) 70 圖 5- 6 粒子平均速度與濃度關係 (error bar為標準差即布朗運動速度) 70 圖 5- 7 布朗運動速度與濃度關係 71 圖 5- 8 濃度與布朗運動擴散關係 71 圖 5- 9 粒徑大小與布朗運動速度比較 72 圖 5- 10 粒徑大小與布朗運動擴散係數比較 72 圖 5- 11 中心速度與邊界布朗運動速度比較 73 圖 5- 12 表面改質與未改質比較布朗運動速度 73 | |
dc.language.iso | zh-TW | |
dc.title | 量測邊界層附近高濃度奈米粒子之布朗運動 | zh_TW |
dc.title | Measurements of Nano Particles’Brownian Motion near the Boundary in a High Concentration Solution | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳光鐘(Kuang-Chong Wu),張正憲(Jeng-Shian Chang) | |
dc.subject.keyword | 布朗運動,生醫感測技術,微粒子追蹤測速儀,螢光相關光譜,高斯分佈, | zh_TW |
dc.subject.keyword | Brownian motion,diffusion,micro particle tracking velocimetry (μPTV),fluorescent,Gaussian distribution, | en |
dc.relation.page | 73 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-08-14 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 應用力學研究所 | zh_TW |
顯示於系所單位: | 應用力學研究所 |
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