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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 吳信志(Shinn-Chih Wu) | |
dc.contributor.author | Guan-Yu Xiao | en |
dc.contributor.author | 蕭冠宇 | zh_TW |
dc.date.accessioned | 2021-06-16T17:37:01Z | - |
dc.date.available | 2022-12-31 | |
dc.date.copyright | 2012-08-19 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-14 | |
dc.identifier.citation | Ashton, B. A., T. D. Allen, C. R. Howlett, C. C. Eaglesom, A. Hattori, and M. Owen. 1980. Formation of bone and cartilage by marrow stromal cells in diffusion chambers in vivo. Clin. Orthop. Relat. Res. 151: 294-307.
Bab, I., B. A. Ashton, D. Gazit, G. Marx, M. C. Williamson, and M. E. Owen. 1986. Kinetics and differentiation of marrow stromal cells in diffusion chambers in vivo. J. Cell Sci. 84: 139-151. Buehr, M., A. McLaren, A. Bartley and S. Darling. 1993. Proliferation and migration of primordial germ cells in We/We mouse embryos. Dev. Dyn. 198: 182-189. Caplan, A. I. 1991. Mesenchymal stem cells. J. Orthop. Res. 9: 641-650. Carpenter, M. K., E. Rosler and M. S. Rao. 2003. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells 5: 79-88. Chamberlain, G., J. Fox, B. Ashton, and J. Middleton. 2007. Concise review: Mesenchymal stem cells: Their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells 25: 2739-2749. Choi, YTaM., F. Atouf and N. Lumelsky. 2004. Adult pancreas generates multipotent stem cells and pancreatic and nonpancreatic progeny. Stem Cells 22(6): 1070-1084. Danner, S., J. Kajahn, C. Geismann, E. Klink, and C. Kruse. 2007. Derivation of oocyte-like cells from a clonal pancreatic stem cell line. Mol. Hum. Reprod. 13: 11-20. De Coppi, P., A. Callegari, A. Chiavegato, L. Gasparotto and M. Piccoli. 2007. Amniotic fluid and bone marrow derived mesenchymal stem cells can be converted to smooth muscle cells in the cryo-injured rat bladder and prevent compensatory hypertrophy of surviving smooth muscle cells. J. Urol. 177: 369-376. De Coppi, P., G. Bartsch, Jr., M. M. Siddiqui, T. Xu and C. C. Santos. 2007. Isolation of amniotic stem cell lines with potential for therapy. Nat. Biotechnol. 25: 100-106. De Gemmis, P., C. Lapucci, M. Bertelli, A. Tognetto and E. Fanin. 2006. A real-time PCR approach to evaluate adipogenic potential of amniotic fluid-derived human mesenchymal stem cells. Stem Cells and Dev. 15: 719–728. Di Nicola, M., C. Carlo-Stella, M. Magni, M. Milanesi, P. D. Longoni, P. Matteucci, S. Grisanti, and A. M. Gianni. 2002. Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli. Blood 99: 3838-3843. Dyce, P. W., L. Wen and J. Li. 2006. In vitro germline potential of stem cells derived from fetal porcine skin. Nat. Cell Biol. 8: 384–390. Dyce, P. W., W. Shen, E. Huynh, H. Shao, D. A. F. Villago’mez, G. M. Kidder, W. A. King and J. Li. 2011. Analysis of oocyte-like cells differentiated from porcine fetal skin-derived stem cells. Stem cells and development 20(5): 1-11. Eslaminejad, M. B., S. Jahangir and N. Aghdami. 2011. Mesenchymal stem cells from murine amniotic fluid as a model for preclinical investigation. Archives of Iranian Medicine 14(2): 96-103. Evans, M. and M. Kaufman. 1981. Establishment in culture of pluripotential cells from mouse embryos. Nature 292: 154-156. Fortune, J. E., G. M. Rivera and M. Y. Yang. 2004. Follicular development: the role of the follicular microenvironment in selection of the dominant follicle. Anim. Reprod. Sci. 82: 109-126. Friedenstein, A. J., U. F. Deriglasova, N. N. Kulagina, A. F. Panasuk, S. F. Rudakowa, E. A. Luria, and I. A. Ruadkow. 1974. Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp. Hematol. 2: 83-92. Gosden, C. M. 1983. Amniotic fluid cell types and culture. Br. Med. Bull. 39: 348-354. Hubner, K., G. Fuhrmann, L. K. Christenson, J. Kehler, R. Reinbold, R. D. L. Fuente, J. Wood, J. F. Strauss III, M. Boiani and H. R. Scholer. 2003. Derivation of oocytes from mouse embryonic stem cells. Science 300: 1251-1256. IntAnker, P. S., S. A. Scherjon, C. Kleijburg-van der Keur, W. A. Noort, F. H. J. Claas, R. Willemze, W. E. Fibbe, and H. H. H. Kanhai. 2003. Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation. Blood 102: 1548-1549. IntAnker, P. S., S. A. Scherjon, D. K. C. Kleijburg-Van,G. M. de Groot-Swings, and F. H. Claas. 2004. Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta. Stem Cells 22: 1338-1345. Johnson, J., J. Bagley, M. Skaznik-Wikiel, H. J. Lee, G. B. Adams, Y. Niikura, K. S. Tschudy, J. C. Tilly, M. L. Cortes, R. Forkert, T. Spitzer, J. Iacomini, D. T. Scadden, and J. L. Tilly. 2005. Oocyte generation in adult mammalian ovaries by putative germ cells in bone marrow and peripheral blood. Cell 122: 303-315. Johnson, J., J. Canning, T. Kaneko, J. K. Pru and J. L. Tilly. 2004. Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature 428: 145-150. Kapit, W and L. M. Elson. 2001. Anatomy coloring book. 2nd Edition, Benjamin Cummings. ISBN-10: 0805350861. Kogler, G, S. Sensken, J. A. Airey, T. Trapp, M. Muschen, N. Feldhahn, S. Liedtke, R. V. Sorg, J. Fischer, C. Rosenbaum. 2004. A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J. Exp. Med. 200(2): 123-135. Kolambkar, Y. M., A. Peister, S. Soker, A. Atala and R. S. Guldberg. 2007. Chondrogenic differentiation of amniotic fluid-derived stem cells. J. Mol. Histol. 38: 405-413. Lavrovsky, Y., C. S. Song, B. Chatterjee and A. K. Roy. 1998. A rapid and reliable PCR-based assay for gene transmission and sex determination in newborn transgenic mice. Transgenic Research 7: 319-320. Lee, H. J., K. Selesniemi, Y. Niikura, T. Niikura, R. Klein, D. M. Dombkowski, and J. L. Tilly. 2007. Bone marrow transplantation generates immature oocytes and rescues long-term fertility in a preclinical mouse model of chemotherapy-induced premature ovarian failure. J. Clin. Oncol. 25: 3198-3204. Lee, K. D., T. K. Kuo, J. Whang-Peng, Y. F. Chung, C. T. Lin, S. H. Chou, J. R. Chen, Y. P. Chen, and O. K. Lee. 2004. In vitro hepatic differentiation of human mesenchymal stem cells. Hepatology 40: 1275-1284. Martin-Rendon, E., D. Sweeney, F. Lu, J. Girdlestone and C. Navarrete. 2008. 5-Azacytidine-treated human mesenchymal stem/progenitor cells derived from umbilical cord, cord blood and bone marrow do not generate cardiomyocytes in vitro at high frequencies. Vox. Sang. 95: 137-148. Matsui, Y. and D. Okamura. 2005. Mechanisms of germ-cell specification in mouse embryos. Bioessays 27: 136-143. McLaren, A. 2003. Primordial germ cells in the mouse. Dev. Biol. 262: 1-15. Menke, D. B., J. Koubova and D. C. Page. 2003. Sexual differentiation of germ cells in XX mouse gonads occurs in an anterior-to-posterior wave. Dev. Biol. 262: 303–312. Meirow, D., H. Biedermann, R. A. Anderson and W. H. Wallace. 2010. Toxicity of chemotherapy and radiation on female reproduction. Clin. Obst. and Gynaecol. 53: 727-739. Milunsky, A. 1979. Amniotic fluid cell culture. Genetic Disorder of the Fetus. New York: Plenum Press, 75. Molyneaux, K. and C. Wylie. 2004. Primordial germ cell migration. Int. J. Dev. Biol. 48: 537-544. Morgan, S., R. A. Anderson, C. Gourley, W. H. Wallace and N. Spears. 2012. How do chemotherapeutic agents damage the ovary? Hum. Reprod. Update. 18(5): 525-535. Novak, I., D. A. Lightfoot, H. Wang, A. Eriksson, E. Mahdy and C. Hoog. 2006. Mouse embryonic stem cells form follicle-like ovarian structures but do not progress through meiosis. Stem Cells 24: 1931-1936. O’Brien, M. J., J. K. Pendola and J. J. Eppig. 2003. A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence. Biol. Reprod. 68:1682-1686. Ortiz-Gonzalez, X. R., C. D. Keene, C. M. Verfaillie and W. C. Low. 2004. Neural induction of adult bone marrow and umbilical cord stem cells. Curr. Neurovasc. Res. 1(3): 207-213. Pepling, M.E. 2006. From primordial germ cell to primordial follicle: mammalian female germ cell development. Genesis 44: 622-632. Prusa, A. R., E. Marton, M. Rosner, G. Bernaschek and M. Hengstschlager. 2003. Oct-4 expressing cells in human amniotic fluid: a new source for stem cell research? Hum. Reprod. 18: 1489-1493. Qing, T., Y. Shi, H. Qin, X. Ye, W. Wei, H. Liu, M. Ding and H. Deng. 2007. Induction of oocyte-like cells from mouse embryonic stem cells by co-culture with ovarian granulosa cells. Differentiation 75: 902-911. Ramiya, V. K., M. Maraist, K. E. Arfors, D. A. Schatz, A. B. Peck and J. G. Cornelius. 2000. Reversal of insulin-dependent diabetes using islets generated in vitro from pancreatic stem cells. Nat. Med. 6(3): 278-282. Rehni, A. K., N. Singh, A. S. Jaggi and M. Singh. 2007. Amniotic fluid derived stem cells ameliorate focal cerebral ischaemia-reperfusion injury induced behavioural deficits in mice. Behav. Brain Res. 183: 95-100. Sanchez-Ramos, J., S. Song, F. Cardozo-Pelaez, C. Hazzi, T. Stedeford, A. Willing, T. B. Freeman, S. Saporta, W. Janssen, N. Patel, D. R. Cooper, and P. R. Sanberg. 2000. Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp. Neurol. 164: 247-256. Senger, P. L. 2003. Pathways to PREGNANCY and PARTURITION. 2nd Edition, Washington State University, Pullman, Washington. ISBN 0-9657648-1-8. Song, S. H., B. M. Kumar, E. J. Kang, Y. M. Lee, T. H. Kim, S. A. Ock, S. L. Lee, B. G. Jeon and G. J. Rho. 2011. Characterization of porcine multipotent stem/stromal cells derived from skin, adipose, and ovarian tissues and their differentiation in vitro into putative oocyte-like cells. Stem Cells Dev. 20(8): 1359-1370. Thomson, J. A. and J. S. Odorico. 2000. Human embryonic stem cell and embryonic germ cell lines. Trends. Biotechnol. 18: 53-57. Underwood, M. A., W. M. Gilbert and M. P. Sherman. 2005. Amniotic fluid: not just fetal urine anymore. J. Perinatol. 25: 341. Watt, F. M. and B. L. Hogan. 2000. Out of Eden: stem cells and their niches. Science 287 (5457): 1427-1430. Zou, K., Z. Yuan, Z. Yang, H. Luo, K. Sun, L. Zhou, J. Xiang, L. Shi, Q. Yu, Y. Zhang, R. Hou and J. Wu. 2009. Production of offspring from a germline stem cell line derived from neonatal ovaries. Nat. Cell Biol. 11(5): 631-636. Zuckerman, S. 1951. The number of oocytes in the mature ovary. Recent. Prog. Horm. Res. 6: 63-108. Zuk, P. A., M. Zhu, P. Ashjian, D. A. De Ugarte, J. I. Huang, H. Mizuno, Z. C. Alfonso, J. K. Fraser, P. Benhaim, and M. H. Hedrick. 2002. Human adipose tissue is a source of multipotent stem cells. Mol. Biol. Cell 13: 4279-4295. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64252 | - |
dc.description.abstract | 就哺乳動物之繁殖生理學而言,一般認為多數雌性哺乳動物在出生前夕,雌性生殖細胞-卵母細胞 (oocytes) 之增生作用即告停止,動物出生後,其卵巢中卵母細胞之數量會隨著年齡增長而逐漸下降,一旦卵母細胞悉數耗盡,雌性動物將會進入更年期 (menopause),而不再具有生育能力。先前研究發現,骨髓來源細胞移植有助於修復彼等因化療藥物 busulfan 和 cyclophosphamide 處理而導致卵巢缺陷的小鼠之生育力。唯有關藉由骨髓移植治療卵巢缺陷之修復機制是透過植入之幹細胞經轉分化成為生殖細胞或卵巢細胞,抑或透過彼等旁泌因子之協助修復所使然,則迄今未明。近年來有研究指出羊水幹細胞具有低免疫原性的特性,可以避免免疫排斥問題,且分化潛能可能優於間葉幹細胞。因此,本研究的目的旨在探討羊水幹細胞是否具有修復雌性生育力的潛能及其修復之可能機制。
本研究使用綠色螢光蛋白質基因轉殖小鼠,取懷孕 11.5 天之胎體為材料,首先分離並建立小鼠羊水幹細胞。結果證明,爰此所建立之小鼠羊水幹細胞不僅具備類似小鼠骨髓間葉幹細胞之固有特性,且其細胞增殖能力較佳。此外,小鼠羊水幹細胞不僅會表現多能性幹細胞之特異性分子標誌-Oct-4,其能於體外誘導分化條件下,且可進一步分化為脂肪細胞、硬骨細胞及軟骨細胞。本研究室之研究證明,在體外誘導分化成為生殖細胞的條件下,其中部分之小鼠羊水幹細胞在形態上會逐漸形成類似於生殖細胞發育的團塊結構,且有表現生殖細胞的特定標誌- DAZL。進一步的分化條件下,這些細胞會形成類濾泡之結構且開始分泌動情素。分化至第25之際,會產生外觀型態類似於卵母細胞之大細胞,此類大細胞會表現生殖細胞之特異性蛋白質-VASA。為謀證實小鼠羊水幹細胞是否具有修復雌性生育力之潛能,本研究建立卵巢缺陷模式小鼠做為宿主,並將表現綠色螢光蛋白質之小鼠羊水幹細胞移植入宿主小鼠之卵巢內。試驗結果證明,受試小鼠於移植羊水幹細胞 4 週後,宿主卵巢內所擁有之發育中濾泡數量顯著高於未經移植細胞之卵巢缺陷小鼠,且閉鎖濾泡數量也顯著下降。此外,宿主小鼠經過細胞移植後,均恢復其部分之生育力,然而結果發現,所有的胚胎都不是源自於移植之小鼠羊水幹細胞。組織免疫螢光染色結果顯示,雖然於移植一個月後,小鼠羊水幹細胞仍然存在於宿主小鼠卵巢內,唯毫無觀察到彼等植入之羊水幹細胞成功被轉分化成為生殖細胞之情事。 綜上合述,本研究證明源自小鼠之羊水幹細胞具備在體外分化成為類生殖細胞之潛能;且於體內試驗中證實,彼等細胞可能是透過分泌旁泌因子之方式進而達到修復卵巢缺陷小鼠生育力之功效。此等試驗結果暗示在臨床上藉由細胞移植策略,達成有效治療女性不孕症之可行性。 | zh_TW |
dc.description.abstract | The dogma of reproductive biology field is that female mammalian loss of germ cell renewal ability before birth, then the reserve of germ cells decreased during postnatal life until exhaustion, resulting in menopause and irreversible ovarian failure. Previous studies demonstrated that bone marrow-derived cells can rescue the fertility of mouse treated with drugs, busulfan and cyclophosphamide, which damage the germ cells in cancer patients. However, there was no evidence shown the restoring pathway of bone marrow-derived cells whether via germ cell differentiation, ovarian cell differentiation or paracrine factors secretion. Recently, it was reported that amniotic fluid stem cells (AFSCs) have low immunogenicity to avoid immunorejection and may have better differentiation ability than mesenchymal stem cells (MSCs). Therefore, we intend to investigate if AFSCs can recover female fertility via germline differentiation.
In this study, we established mouse AFSCs (mAFSCs) which expressing foreign enhanced green fluorescence protein (EGFP) that derived from EGFP bearing mouse conceptus (11.5 days). These mAFSCs exhibited the characteristics similar to bone marrow MSCs, and have the higher proliferation ability. In addition, mAFSCs express the pluripotent specific marker-Oct 4, and could differentiate into adipocyte, osteocyte and chondrocyte under appropriate condition. When mAFSCs were induced to differentiate into female germ cell, a subpopulation of these cells detached to each other gradually and formed aggregates resembling female germ cell formation. These cells subsequently expressed the germ cell marker-DAZL under induction condition. On further differentiation, these cells which formed the follicle-like structure that secreted estradiol under gonadotropin stimulation. Twenty-five days after differentiation induction, a few cells showed oocyte-like morphology and expressed the germ cell specific marker-VASA as well. To evaluate whether mAFSCs can recover female fertility, we use the ovarian failure model mice as recipients, and then transplanted EGFP bearing mAFSCs (EGFP-mAFSCs) into ovary of recipient mice. Four weeks after cell transplantation, numbers of developing follicle within host ovaries were significant higher than ovarian failure model mice, and numbers of atretic follicle were significant decreased. In addition, the fertility of all recipients was restored, but there was no fetus derived from EGFP-mAFSCs. Although EGFP-mAFSCs was observed within ovary after one month of transplantation, there was no evidence shown that mAFSCs differentiated into germ cell. Collectively, this study demonstrated that mAFSCs have the potential to differentiate into germline in vitro. In addition, in vivo trial verified that mAFSCs can rescue the fertility in ovarian failure mice may via paracrine factors secretion. These findings implicated that the potentiality of clinical cell-transplantation therapy for the treatment of infertility. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T17:37:01Z (GMT). No. of bitstreams: 1 ntu-101-R99626015-1.pdf: 2710737 bytes, checksum: 64a07ac4d193f3195c820abda8d646ec (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 口試委員會審定書
謝誌 i 中文摘要 ii ABSTRACT iv 目次 vi 圖次 ix 表次 xi 第1章 前言 1 第2章 文獻探討 2 2.1 雌性生殖系統 2 2.1.1 卵巢構造 2 2.1.2 卵巢細胞 3 2.1.3 生殖細胞 3 2.2 動情周期 5 2.3 化療藥物引起之女性不孕症 9 2.4 幹細胞 11 2.4.1 幹細胞之簡介 11 2.4.2 幹細胞的來源 11 2.4.3 羊水幹細胞 13 2.5 運用幹細胞體外分化為雌性生殖細胞之研究 15 2.5.1 胚幹細胞體外分化為雌性生殖細胞之研究 15 2.5.2 成體幹細胞體外分化為雌性生殖細胞之研究 16 2.6 應用細胞移植治療卵巢缺陷模式動物之研究 17 第3章 試驗研究 18 3.1 綠色螢光小鼠羊水幹細胞之分離和建立 18 3.1.1 前言 18 3.1.2 材料與方法 19 3.1.3 結果 24 3.1.4 討論 30 3.2 綠色螢光小鼠羊水幹細胞體外誘導分化為雌性生殖細胞之研究 31 3.2.1 前言 31 3.2.2 材料與方法 32 3.2.3 結果 35 3.2.4 討論 39 3.3 綠色螢光小鼠羊水幹細胞修復化療誘導受損小鼠卵巢功能之研究 40 3.3.1 前言 40 3.3.2 材料與方法 41 3.3.3 結果 45 3.3.4 討論 57 第4章 綜合討論 58 第5章 結論 60 第6章 未來展望 61 REFERENCES 62 | |
dc.language.iso | zh-TW | |
dc.title | 小鼠羊水幹細胞修復卵巢缺陷小鼠生育力之潛能 | zh_TW |
dc.title | The Potential of Mouse Amniotic Fluid Stem Cells to Rescue Fertility of Ovarian Failure Mice | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鄭登貴(Teng-Kuei Cheng),劉逸軒(I-Hsuan Liu),林劭品(Shau-Ping Lin),宋麗英(Li-Ying Sung) | |
dc.subject.keyword | 羊水幹細胞,再生醫學,卵巢缺陷, | zh_TW |
dc.subject.keyword | Amniotic fluid stem cells,Regenerative medicine,Ovarian failure, | en |
dc.relation.page | 68 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-08-15 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 動物科學技術學研究所 | zh_TW |
顯示於系所單位: | 動物科學技術學系 |
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