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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64182完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 郭柏齡(Po-Ling Kuo) | |
| dc.contributor.author | Chih-Tim Hsu | en |
| dc.contributor.author | 許至廷 | zh_TW |
| dc.date.accessioned | 2021-06-16T17:33:44Z | - |
| dc.date.available | 2021-02-22 | |
| dc.date.copyright | 2021-02-22 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-02-04 | |
| dc.identifier.citation | [1] Seuring T, Archangelidi O, Suhrcke M. The economic costs of type 2 diabetes: a global systematic review. Pharmacoeconomics 2015; 33: 811–31. [2] Adam L. Burrack, imageTijana Martinov and imageBrian T. Fife. T Cell-Mediated Beta Cell Destruction: Autoimmunity and Alloimmunity in the Context of Type 1 Diabetes. Front. Endocrinol., 05 December 2017. [3] Karges W, Hammond-McKibben D, Gaedigk R, Shibuya N, Cheung R, Dosch HM. Loss of self-tolerance to ICA69 in nonobese diabetic mice. Diabetes (1997) 46(10):1548–56. doi:10.2337/diabetes.46.10.1548. [4] OKON U. A., Owo D.U., Udokang N.E., Udobang J.A., Ekpenyong C.E. Oral Administration of Aqueous Leaf Extract of Ocimum Gratissimum Ameliorates Polyphagia, Polydipsia and Weight Loss in Streptozotocin-Induced Diabetic Rats. American Journal of Medicine and Medical Sciences 2012. [5] Sudesna Chatterjee MD Prof Kamlesh Khunti PhD Prof Melanie JDaviesM. Type 2 diabetes. Science Direct 3–9 June 2017. [6] Harold Brem, Marjana Tomic-Canic.Cellular and molecular basis of wound healing in diabetes. JCL, May1, 2007. [7] Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. The Journal of International Medical Research, 2009. [8] Sharp, A. Clark, J. Diabetes and its impact on wound healing, Nursing Standard. 2011, 25, 45, 41-47. [9] Timmermans, F.; Plum, J.; Yoder, M.C.; Ingram, D.A.; Vandekerckhove, B.; Case, J. Endothelial progenitor cells: Identity defined? J. Cell. Mol. Med. 2009, 13, 87–102. [10] Bin Zhang, Ronne Wee Yeh Yeo, Kok Hian Tan and Sai Kiang Lim, Focus on Extracellular Vesicles: Therapeutic Potential of Stem Cell-Derived Extracellular Vesicles. Int. J. Mol. Sci. 2016. [11] James R. Edgar, Q A: What are exosomes, exactly? BMC Biol. 2016. [12] Raghu Kalluri, Valerie S. LeBleu, The biology, function, and biomedical applications of exosomes, Science, 07 Feb 2020. [13] Poren Kong, Xiaoyun Xie , Fang Li, Yang Liu, Yingli Lu, Placenta mesenchymal stem cell accelerates wound healing by enhancing angiogenesis in diabetic Goto-Kakizaki (GK) rats, Biochem Biophys Res Commun. 2013 Aug 23. [14] Paz de la Torre, María Jesús Pérez-Lorenzo and Ana I. Flores, Human Placenta-Derived Mesenchymal Stromal Cells: A Review from Basic Research to Clinical Applications, Stromal Cells - Structure, Function, and Therapeutic Implications, 2019. [15] 林泰元、陳耀昌、黃彥華. (新型第M462274 號) 一種可由特定組織中取得成體幹細胞以製成生物製劑系統。中華民國新型專利2013.9.21 ~ 2023.02.20. [16] 林泰元、陳耀昌、侯勝茂、嚴孟祿、黃彥華、吳孟學. (發明第I419971 號)一種成體幹細胞之組織分解、細胞黏附與萃取寄培養技術。中華民國發明專利 2013.12.21 ~ 2031.09.27. [17] Danyang Zhao, Zhencheng Yu, Yun Li, Yu Wang, Qingfeng Li, Dong Han, GelMA combined with sustained release of HUVECs derived exosomes for promoting cutaneous wound healing and facilitating skin Regeneration, Journal of Molecular Histology, 2020 Jun. [18] Koehler, J., Brandl, F. P., Goepferich, A. M. Hydrogel wound dressings for bioactive treatment of acute and chronic wounds. European Polymer Journal, 2018. [19] Kan Yue, Grissel Trujillo-de Santiago, Mario Moisés Alvarez, Ali Tamayol, Nasim Annabi, and Ali Khademhosseini. Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials, 2015. [20] A.I. Van Den Bulcke, B. Bogdanov, N. De Rooze, E.H. Schacht, M. Cornelissen, H. Berghmans, Structural and rheological properties of methacrylamide modified gelatin hydrogels, Biomacromolecules, 2000. [21] Mingyue Sun, Xiaoting Sun, Ziyuan Wang, Shuyu Guo, Guangjiao Yu, and Huazhe Yang, Synthesis and Properties of Gelatin Methacryloyl (GelMA) Hydrogels and Their Recent Applications in Load-Bearing Tissue, Polymers, 2018 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64182 | - |
| dc.description.abstract | 細胞治療的興起也帶動了大家對細胞外泌體治療的關注，近幾年來間質幹細胞在細胞治療中已表現出免疫調節的能力和治療的能力，而這些本領被許多人認為是來自間質幹細胞所分泌出的外泌體。然而想將這些外泌體散佈到傷口上被眾人所低估，因此本研究嘗試用水凝膠將外泌體可以送至傷口，而這水凝膠是可以客製化並應用在各種不同的臨床需求，我們希望這種傷口貼布設計可以在未來對傷患有所幫助。 | zh_TW |
| dc.description.abstract | With the rise of cell therapy, exosome therapy had also gained many attentions in the field of biomedical engineering. In recent years, mesenchymal stem cells have been shown to possess immunomodulation and regeneration abilities in cell therapy. It was believed that these capabilities came from their own exosomes. However, the most optimal way of delivering exosomes to the wound site had been underrated. Therefore, experiments were conducted in this study to deliver exosomes to the wound with the help of hydrogels, which can be fabricated on customization and applied to literally any shape of wound. We hope that the dressings designed could be utilized in clinical use in the near future for patients in need | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T17:33:44Z (GMT). No. of bitstreams: 1 U0001-0202202118154500.pdf: 4605690 bytes, checksum: a7d2d030cccefd90a973226f7efbea0b (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 口試委員會審書..................................................................... i 誌謝............................................................................. ii 中文摘要..........................................................................iii Abstract..........................................................................iv Content .......................................................................... v Chapter 1 Introduction............................................................. 1 Chapter 2 Materials and Methods................................................... 11 2.1 Cell culture.................................................................. 11 2.2 GelMA fabrication ............................................................ 11 2.3 Purification and Nano Tracking Analysis of Pc-MSC derived exosomes............ 12 2.4 Western Blot.................................................................. 13 2.5 Transmission electron microscopy.............................................. 13 2.6 In-vitro wound healing assay ................................................. 14 2.7 Dressing construction......................................................... 16 2.8 Diabetic rat wound models and treatment ...................................... 17 2.9 Formalin-Fixed Paraffin Embedded Tissue ...................................... 19 2.10 Immunohistochemistry staining and quantification ............................ 20 Chapter 3 Results and Duscussion ................................................. 22 3.1 Exosome isolation and characterization........................................ 22 3.2 Pc-MSC Exosomes probably promote migration in vitro........................... 23 3.3 Evaluation of wound healing treated with Exosomes in vivo..................... 25 3.4 Immunohistochemistry staining ................................................ 39 3.5 Gelma improvement for exosome release......................................... 51 Chapter 4 Conclusion and Future works............................................. 54 Chapter 5 Reference .............................................................. 56 Chapter 6 Supplement ............................................................. 59 | |
| dc.language.iso | en | |
| dc.subject | 間質幹細胞外泌體 | zh_TW |
| dc.subject | 3D 列印 | zh_TW |
| dc.subject | 甲基丙烯酰基明膠 | zh_TW |
| dc.subject | 傷口貼布 | zh_TW |
| dc.subject | 傷口癒 合 | zh_TW |
| dc.subject | GelMA hydrogel | en |
| dc.subject | Mesenchymal stem cells Exosomes | en |
| dc.subject | healing patch | en |
| dc.subject | 3D printing | en |
| dc.subject | wound healing | en |
| dc.title | 幹細胞外泌體結合3D生物列印GelMA材料對STZ誘導糖尿病大鼠之傷口癒合影響 | zh_TW |
| dc.title | Effect of stem-cell-exosome-embedded GelMA hydrogel fabricated by 3D bioprinting on wound healing in streptozotocin-induced diabetic rats | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 109-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 曾宇鳳(Yufeng Jane Tseng) | |
| dc.contributor.oralexamcommittee | 鄭乃禎(Nai-chen Cheng) | |
| dc.subject.keyword | 間質幹細胞外泌體,甲基丙烯酰基明膠,傷口貼布,3D 列印,傷口癒 合, | zh_TW |
| dc.subject.keyword | Mesenchymal stem cells Exosomes,GelMA hydrogel,healing patch,3D printing,wound healing, | en |
| dc.relation.page | 73 | |
| dc.identifier.doi | 10.6342/NTU202100407 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2021-02-05 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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