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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳示國(Shih-Kuo Chen) | |
| dc.contributor.author | Tsung-Hao Lu | en |
| dc.contributor.author | 盧從浩 | zh_TW |
| dc.date.accessioned | 2021-06-16T17:33:14Z | - |
| dc.date.available | 2020-03-05 | |
| dc.date.copyright | 2020-03-05 | |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2020-03-02 | |
| dc.identifier.citation | Arumugam, M., J. Raes, E. Pelletier, D. Le Paslier, T. Yamada, D. R. Mende, G. R. Fernandes, J. Tap, T. Bruls, J. M. Batto, M. Bertalan, N. Borruel, F. Casellas, L. Fernandez, L. Gautier, T. Hansen, M. Hattori, T. Hayashi, M. Kleerebezem, K. Kurokawa, M. Leclerc, F. Levenez, C. Manichanh, H. B. Nielsen, T. Nielsen, N. Pons, J. Poulain, J. Qin, T. Sicheritz-Ponten, S. Tims, D. Torrents, E. Ugarte, E. G. Zoetendal, J. Wang, F. Guarner, O. Pedersen, W. M. de Vos, S. Brunak, J. Dore, M. Antolin, F. Artiguenave, H. M. Blottiere, M. Almeida, C. Brechot, C. Cara, C. Chervaux, A. Cultrone, C. Delorme, G. Denariaz, R. Dervyn, K. U. Foerstner, C. Friss, M. van de Guchte, E. Guedon, F. Haimet, W. Huber, J. van Hylckama-Vlieg, A. Jamet, C. Juste, G. Kaci, J. Knol, O. Lakhdari, S. Layec, K. Le Roux, E. Maguin, A. Merieux, R. Melo Minardi, C. M'Rini, J. Muller, R. Oozeer, J. Parkhill, P. Renault, M. Rescigno, N. Sanchez, S. Sunagawa, A. Torrejon, K. Turner, G. Vandemeulebrouck, E. Varela, Y. Winogradsky, G. Zeller, J. Weissenbach, S. D. Ehrlich and P. Bork (2011). Enterotypes of the human gut microbiome. Nature, 473(7346), 174-180. doi:10.1038/nature09944
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64171 | - |
| dc.description.abstract | 自從19世紀，電燈泡的發明為人們帶來許多的便利，然而它也卻引發了許多生理代謝的健康問題。在先前的文獻發現，光害嚴重的程度與人類肥胖有正相關的關係。然而，光線對如何影響代謝問題的機制不甚了解。其中腸道菌的研究日益增加，已經有許多文獻發現到腸道菌相可以受許多的外在變因給調控，並且腸道菌跟宿主的代謝功能息息相關。故我們想要利用操弄環境光線的變化以及使用不同基因型的老鼠，來去解答光線所引起的肥胖是否藉由改變腸道菌相所造成。我們的研究發現，夜晚光線確實會造成肥胖以及血糖耐受性降低的症狀，而這些症狀必須搭配吃食高脂飼料才會出現。同時，將來自夜晚照光小鼠的糞便樣本，管餵給無菌小鼠的實驗發現到腸道菌並不參與夜晚光線所引起的肥胖當中。而我們的實驗發現到了光線以及腸道菌相週期性的關係。外在光線的週期是調控腸道週期的關鍵因素，而不是宿主本身的生理時鐘。本研究排除了腸道菌為光線所引發的肥胖變因的可能，並且也提供了外在光線調控腸道菌相週期性的全新觀點。 | zh_TW |
| dc.description.abstract | In 19th century, Thomas Edison invented light bulbs which benefits human society. However, artificial light gives rise to many metabolic problems. Depending on previous studies, the severity of the light pollution is correlated with obesity. However, the mechanism of light-induced metabolic disorder remains unknown. many papers have discovered that many factors can modulate the microbial profile, and gut microbiome is relevant to metabolism of the host. The purpose of this study is to investigate the connection between light-induced obesity and gut microbiome by manipulating different light conditions and gene modified mice. Our findings suggested that dim light at night combined with high fat diet induced the metabolic deficits. Additionally, gut microbiome did not involve in light-induced obesity based on the result from germ-free mice transplanted with feces. For the relationship between light and gut microbiome, our work showed that external light cues are the critical factor to regulate the microbial rhythmicity instead of the output of the central clock. In conclusion, this study not only excludes the possibility that gut microbiome takes part in light-induced metabolic disorders but provides a whole new idea that external light can regulate microbial oscillation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T17:33:14Z (GMT). No. of bitstreams: 1 ntu-108-R06b21012-1.pdf: 3034885 bytes, checksum: 5ce9fb386b72b0a045fd3d9aae7798e1 (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 口試委員審定書 .....i
謝誌 .....ii 摘要 .....iv Abstract .....v Contents .....vi Chapter1 Introduction .....1 1.1 The effect of artificial light on physiological functions .....1 1.2 Intrinsically photosensitive retinal ganglion cells (ipRGCs) .....2 1.2.1 Subtypes of ipRGCs .....3 1.3 Circadian rhythm .....4 1.3.1 Molecular mechanism of circadian rhythm .....4 1.3.2 Suprachiasmatic nucleus (SCN) .....5 1.4 Gut microbiome .....6 1.4.1 The composition of gut microbiome .....6 1.4.2 Gut microbiome and obesity related problems .....7 1.4.3 Gut microbiome oscillation .....7 Statement of Purpose .....9 Chapter II Materials and Methods .....11 2.1 animals .....11 2.2 genotyping .....11 2.2.1 DNA extraction .....11 2.2.2 Polymerase chain reaction (PCR) .....12 2.3 Metabolism test .....12 2.3.1 Glucose tolerance test (GTT) .....12 2.3.2 Insulin tolerance test (ITT) .....12 2.4 Immunostaining .....13 2.4.1 Brain collection and brain dissection .....13 2.4.2 PERIOD2 ABC staining .....13 2.5 Metagenomic library preparation for Illumina sequencing .....13 2.5.1 Gut microbe DNA extraction .....14 2.5.2 16S metagenomic library preparation .....15 2.5.3 Library quality check and pooling .....17 2.5.4 Next generation sequencing (NGS) .....17 2.6 Microbiota sequence analysis .....18 2.6.1 Sequence assembling and classification .....18 2.6.2 Microbiota analysis .....19 2.7 Experimental design .....21 2.8 Statistical analysis .....21 Chapter III Results .....23 3.1 Dim light at night induced obesity and metabolic deficits in wild type mice .....23 3.2 Gut microbiome does not take part in light-induced obesity .....24 3.3 dLAN and diet influence the composition of gut microbiome .....25 3.4 External light manipulation, regardless of the diet, interrupts the microbial oscillation .....26 3.5 Brn3b positive ipRGCs is necessary for microbial oscillation but not for composition of gut microbiome .....28 3.6 The circadian rhythm in central clock modulates the effect of external light on gut microbiome .....29 Chapter IV Discussion .....31 4.1 Light-induced obesity is not relevant to the composition of gut microbiome .....31 4.2 F:B ratio, which is higher in dLAN group, might be the compensation for the WT mice housed under dLAN .....32 4.3 The experiment of Bmal1fx/fx mice fails to explain if the externa light-dark cycle could directly regulate the microbial oscillation .....32 4.4 Further detailed pathway can be investigated by knocking out the BMAL1 in intestine epithelial cells .....33 Significance of the work .....35 Reference .....36 Figures .....44 Figure 1. Experimental scheme and design .....44 Figure 2. Metabolic status of WT mice fed with HFD .....45 Figure 3. Glucose tolerance test of WT mice .....46 Figure 4. Metabolic status of fecal transplanataion germ-free mice .....47 Figure 5. Weight gain of norepinephrine beta-receptor antagonist-treatedmice .....48 Figure 6. Body weight of mice fed with regular diet .....49 Figure 7. Gut microbiome profile of WT mice fed with HFD .....50 Figure 8. gut microbiome profile of WT mice fed with regulate food .....51 Figure 9. Oscillating OTUs percentage in WT mice fed with HFD and normal chow .....52 Figure 10. Experimental design of constant dark experiments .....53 Figure 11. gut microbiome profile of WT mice housed under DD fed with HFD .....54 Figure 12. Oscillating OTUs percentage in WT mice fed with HFD .....55 Figure 13. Gut microbiome profile and oscillating OTUs percentage of Brn3bZ-DTA mice ...56 Figure 14. Experimental design of Bmal1fx/fx mice .....57 Figure 15. Dissection of brain slice with target of the SCN from Bmal1fx/fx mice .....58 Figure 16. The actogram of the Bmal1fx/fx mice with the injection of CRE virus .....59 Figure 15. Gut microbiome profile and oscillating OTUs percentage of Bmal1fx/fx mice injected with GFP into SCN as control group .....60 Figure 16. Gut microbiome profile and oscillating OTUs percentage of Bmal1fx/fx mice injected with CRE and GFP into SCN to knock out the central clock .....61 Tables .....62 Appendix I codes used for 16S metagenomic analysis .....63 Appendix II Absteact and Poster .....64 | |
| dc.language.iso | en | |
| dc.subject | 腸道菌 | zh_TW |
| dc.subject | 夜晚光線 | zh_TW |
| dc.subject | 生理時鐘 | zh_TW |
| dc.subject | 自主感光神經節細胞 | zh_TW |
| dc.subject | 次世代定序 | zh_TW |
| dc.subject | gut microbiome | en |
| dc.subject | dim light at night | en |
| dc.subject | circadian rhythm | en |
| dc.subject | ipRGCs | en |
| dc.subject | NGS | en |
| dc.title | 外在光線影響身體代謝以及腸道菌相 | zh_TW |
| dc.title | External light influences metabolic status and gut microbiome profile | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 周銘翊(Ming-Yi Chou),江皓森(Hao-Sen Chiang),楊世斌(Shi-Bing Yang) | |
| dc.subject.keyword | 腸道菌,夜晚光線,生理時鐘,自主感光神經節細胞,次世代定序, | zh_TW |
| dc.subject.keyword | gut microbiome,dim light at night,circadian rhythm,ipRGCs,NGS, | en |
| dc.relation.page | 69 | |
| dc.identifier.doi | 10.6342/NTU202000653 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2020-03-02 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| 顯示於系所單位： | 生命科學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-108-1.pdf 未授權公開取用 | 2.96 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。