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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊台鴻 | |
| dc.contributor.author | Tsung-Yu Hsieh | en |
| dc.contributor.author | 謝宗佑 | zh_TW |
| dc.date.accessioned | 2021-06-16T17:29:47Z | - |
| dc.date.available | 2012-08-22 | |
| dc.date.copyright | 2012-08-22 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-16 | |
| dc.identifier.citation | 1. Gimble, J. M. (2003) Adipose tissue-derived therapeutics, Expert Opin Biol Th 3, 705-713.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64095 | - |
| dc.description.abstract | 脂肪幹細胞應用於皮膚以及傷口的修復已有可觀的成效，因此，對於面臨傷口延遲癒合問題的糖尿病人來說，自體脂肪幹細胞的移植成為一種可行的治療方式。 然而很多研究指出，長期的高血糖狀態會造成許多器官，以及細胞的功能受到損害。 因此，得自糖尿病人的脂肪幹細胞是否適合醫療應用，逐漸地受到關注。 我們使用一個體外的模型來探討長期培養下，培養液中的葡萄糖濃度對脂肪幹細胞細的胞功能之影響。 藉此我們可以簡化與脂肪幹細胞受損有關的因素，並且更專注於高血糖所帶來的結果。
我們發現，高濃度葡萄糖的培養環境會誘使脂肪幹細胞的細胞老化；抑制細胞生長、細胞遷移能力、細胞存活率，以及對於低氧狀態的反應能力。 為了更進一步的探討這些細胞功能的受損是否是可逆的，我們將培養於高葡萄糖環境下脂肪幹細胞轉移至低葡萄糖濃度下培養。 而我們發現，這些細胞的細胞活性有顯著的回復。 總結來說，實驗結果顯示低葡萄糖濃度的處理，能夠增進受到高葡萄糖濃度損害的脂肪幹細胞的細胞活性。 | zh_TW |
| dc.description.abstract | Adipose-derived stem cells (ASCs) have been used in skin and wound repair with satisfactory results, therefore, autologous transplantation of ASCs becomes a treatment option for diabetes patients who facing the problem of delayed wound healing. However, many reports revealed that prolonged hyperglycemia damaged several organs and functions of many cell types, thus raised a concern that whether ASCs from diabetes patients suitable for medical applications. To simplify the factors involved in impaired ASCs activity and to concentrate on the results of hyperglycemia, we provided an in vitro model to investigate the effect of culture medium glucose concentration on ASCs functions during long-term culture.
We found induced cell senescence, decreased cell proliferation, migration, survival rate and response to hypoxia were displayed in ASC under high glucose condition. To further study whether these dysfunction were reversible, ASCs cultured under high glucose condition were then changed into low-glucose medium, and a significant recovery of cellular activities was observed. Collectively, these data suggested that low-glucose treatment improves the functions of ASCs impaired by high glucose concentration. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T17:29:47Z (GMT). No. of bitstreams: 1 ntu-101-R99548010-1.pdf: 11435461 bytes, checksum: cd7d099f00ffe6de913d4faa69fb4f28 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | Contents I
Figures III 中文摘要 IV Abstract V Chapter 1. Introduction 1 1-2 ASCs in applications to wound reparation 3 1-3 Chronic wounds and delayed healing in diabetes 4 1-4 Cellular dysfunction due to hyperglycemia 7 1-5 Hypothesis and design of experiment 8 Chapter 2. Materials and Methods 9 2-1 Cell Culture 9 2-2 Long-term treatment of ASCs with high/low glucose 10 2-3 CD markers detection 11 2-4 Differentiation of ASCs 12 2-5 Cell proliferation 13 2-6 Cell cycle analysis 14 2-7 SA-β-Galactosidase activity staining 15 2-8 ASCs migration 15 2-9 Cell survival 16 2-10 Real-time quantitative polymerase chainreaction (qPCR) 17 2-11 Western blot 19 2-12 Statistical Analysis 20 Chapter 3. Results 21 3-1 ASCs expansion and characterization 21 3-1 Effect of glucose on ASCs proliferation 23 3-3 Effect of glucose on ASC senescence 25 3-4 Effect of glucose on the expression of pluripotent marker genes 26 3-5 Effect of glucose on ASCs migration ability 27 3-6 Effect of glucose on ASCs survival 28 3-7 Effect of glucose on ASCs paracrine and response to hypoxia 29 Chapter 4. Discussion 31 Chapter 5. Conclusion 35 References 36 Figure 41 Fig. I. Scheme for processing of adipose tissue and isolation of adipose-derived stem cells 3 Fig. II. Overview of growth factor involvement in normal epidermal wound healing 5 Fig. III. Stages of wound repair 5 Fig. IV. Mechanisms of wound healing in healthy people versus people with diabetes 6 Fig. 1. Surface antigen analysis of ASCs after cultured 49 days under high glucose 41 Fig. 2. Phase contract photograph of adipogenesis and osteogenesis of ASCs. 43 Fig. 3. Morphology of ASCs 44 Fig. 4. Cell proliferation of ASCs 45 Fig. 5. Analysis of ASCs senescence. 47 Fig. 6. The expression of p21. 48 Fig. 7. The protein expression of pluripotent-related transcriptional factors 49 Fig. 8. Cell migration of ASCs was measured by in vitro scratch assay 50 Fig. 9. Survival rate of ASCs was determined by LDH assay. 51 Fig. 10. Analysis of ASCs paracrine and ECM production activity response to hypoxia. 52 | |
| dc.language.iso | en | |
| dc.subject | 傷口修復 | zh_TW |
| dc.subject | 低氧狀態 | zh_TW |
| dc.subject | 脂肪幹細胞 | zh_TW |
| dc.subject | 葡萄糖 | zh_TW |
| dc.subject | Glucose | en |
| dc.subject | Adipose-derived stem cells | en |
| dc.subject | Hypoxia | en |
| dc.subject | Wound Healing | en |
| dc.title | 低葡萄糖濃度處理以增進受損之脂肪幹細胞活性之研究 | zh_TW |
| dc.title | tudy of the Improvement of Impaired Adipose-derived Stem Cells Activities by Low-glucose | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李弘元,胡威文 | |
| dc.subject.keyword | 脂肪幹細胞,葡萄糖,低氧狀態,傷口修復, | zh_TW |
| dc.subject.keyword | Adipose-derived stem cells,Glucose,Hypoxia,Wound Healing, | en |
| dc.relation.page | 53 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-08-16 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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