請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63959完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林?德(Ta-Te Lin) | |
| dc.contributor.author | Chiang-Yi Yang | en |
| dc.contributor.author | 楊江益 | zh_TW |
| dc.date.accessioned | 2021-06-16T17:24:27Z | - |
| dc.date.available | 2013-08-19 | |
| dc.date.copyright | 2012-08-19 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-16 | |
| dc.identifier.citation | Acker, J. P., J. A. W. Elliott, and L. E. McGann. 2001. Intercellular ice propagation: experimental evidence for ice growth through membrane pores. Biophys. J. 81:1389-1397.
Acker, J. P.,and L. E. McGann. 2003. Protective effect of intracelluar ice during freezing. Cryobiology 46:197-202. Al-Hasani, S., K. Kirsch, J. Diedrich, S. Blanke, H. Van der Ven, and D. Krebs. 1989. Successful embryo transfer of cryopreserved and in vitro fertilized rabbit oocytes. Hum. Reprod. 4: 77-79. Aman, R. R., and J. E. Parks. 1994. Effects of cooling and rewarming on the meiotic spindle and chromosomes of in vitro-matured bovine oocytes. Biol. Reprod. 50:103–110. Ambrosini, G., A. Andrisani, E. Porcu, Z. Rebellato, A. Revelli, D. Caserta, E. Cosmi, R. Marci, and M. Moscarini. 2006. Oocytes cryopreservation: State of art. Reprod. Toxicol. 22: 250-262. Anandarajah, K., G. M. Perumal, M. Sommerfeld, and Q. Hu. 2011. Induced freezing and desiccation tolerance in the microalgae wild type nannochloropsis and scenedesmus dimorphus. Aust. J. Basic Appl. Sci. 5: 678-686. Arakawa, T., J. F. Carpenter, Y. A. Kita, and J. H. Crowe. 1990. The basis of toxicity of certain cryoprotectants: a hypothesis. Cryobiology 27: 401-415. Barbara, G., K. K. Lucyna, R. Bożenna, B. Michał, and S. Zdzisław. 2007. Survival of bovine fibroblasts and cumulus cells after vitrifiaction. CryoLetters 28(4): 271-280. Candy, C. J., M. J. Wood, D. G.. Whittingham, J. A. Merriman, and N. Choudhury. 1994. Cryopreservation of immature mouse oocytes. Hum. Reprod. 9: 1738-1742. Chao, N. H., and I. C. Liao. 2001. Cryopreservation of finfish and shellfish gametes and embryos. Aquaculture 197: 161-189. Chen, H. C. 1984. Recent Innovation in cultivation of edible molluscs in Taiwan, with special reference to the small abalone Haliotis Diversicolor and the hard clam Meretrix Lusoria. Aquaculture 39: 11-27. Chiang, Y. S. 2002. Development and application of a TEC-cryomicroscope. Thesis. National Taiwan University, Taiwan. Didon, B. A., D. Pomp, M. J. Martin, G. E. Homanics, and C. L. 1990. Markert. Observation on the cooling and cryopreservation of pig oocytes at the germinal vesicle stage. J. Anim. Sci. 68: 2803-2810. Diller, K. R., and E. G. Cravalho. 1970. A cryomicroscope for the study of freezing and thawing processes in biological cells. Cryobiology 7: 191-199. Dong, Q., C. Huang, B. Eudeline, and T. R. Tiersch. 2005. Systematic factor optimization for cryopreservation of shipped sperm samples of diploid Pacific Oysters, Crassostrea gigas. Cryobiology 51: 176-197. Dulbecco, R. and M. Vogt.1954. Plaque formation and isolation of pure lines with poliomyelitis viruses. J. Exp. Med. 99: 167-182. Fabbri, R., E. Porcu, T. Marsella, G. Rocchetta, S. Venturoli, and C. Flamigni. 2001. Human oocyte cryopreservation: new perspectives regarding oocyte survival. Hum Reprod. 16: 411-416. Fishery Agency. Fisheries Yearbook. Taiwan, R.O.C 1999. Fujihira, T., R. Kishida, and Y. Fukui. 2004. Developmental capacity of vitrified immature porcine oocytes following ISCI: effect of cytochalasin B and cryoprotectant. Cryobiology 49: 286-290. Funahashi, H., N. H. Kim, T. T. Stumpf, T. C. Cantley, and B. N. Day. 1996. Presence of organic osmolytes in maturation medium enhances cytoplasmic maturation of porcine oocytes. Biol. Reprod. 54: 1412-1419. Hara, K., Y. Abe, N. Kumada, N. Aono, J. Kobayashi, H. Matsumoto, H. Sasada, and E. Sato .2005. Extrusion and removal of lipid from the cytoplasm of porcine oocytes at the germinal vesicle stage: Centrifugation under hypertonic conditions influences vitrification. Cryobiology 50: 216-222. Huang, W. T., C. K. Wallenhorst, S. Wallenhorst, and W. Holtz. 2002. Transfer of porcine embryos through mini-laparotomy. Theriogenology 57: 1533-1537. Huang, W. T., H. C. Yang, T. L. Lee, and P. C. Tang .2006. Cryopreservation of pig oocytes. J. Agri. Assoc. China. 7(4), 343-362. Isachenko, V., C. Soler, E. Isachenko, F. Perez-Sanchez, and V. Grishchenko. 1998. Vitrification of immature porcine oocytes: effects of lipid droplets, temperature, cytoskeleton, and addition and removal of cryoprotectant. Cryobiology 36: 250–253. Isachenko, V., M. Montag, E. Isachenko, S. Dessole, F. Nawroth and H. Van Der Ven . 2006. Aseptic vitrification of human germinal vesicle oocytesusing dimethyl sulfoxide as a cryoprotectant. Fertil. Steril. 85: 741-747. Jian J.K., and R. J. Paulson. 2006. Oocyte cryopreservation. Fertil. Steril. 86:1037-1046. Ju, J. A. 2007. The effects of injecting c-mos siRNA on maturation, fertilization and development of porcine oocyte.Thesis. National ILan University, Taiwan. Karlsson, J. O. M., E. G. Cravalho, and M. Toner. 1993. Intracelluar ice formation: causes and consequences. CryoLetters 14: 323-334. Karlsson, J. O. M., A. Erogle, T. L. Toth, E. G. Cravalho, and M. Toner. 1996. Fertilization and development of mouse oocytes cryopreserved using a theoretically optimized protocol. Hum. Reprod. 11(6): 1296-1305. Karlsson, J. O. M. 2010. Effects of solution composition on the theoretical prediction of ice nucleation kinetics and thermodynamics. Cryobiology 60: 43-51. Katkov, I. I., and A. G. Pogorelov. 2007. Influence of exposure to vitrification solutions on 2-cell mouse embryos: II. Osmotic effects or chemical toxicity?. CryoLetters 28(6): 409-427. Keller, E. R. J. 2005. Improvement of cryopreservation results in garlic using low temperature preculture and high-quality in vitro plantlets. CryoLetters 26(6): 357-366. Kim, G. A., H. Y. Kim, J. W. Kim, G. Lee, E. Lee, J. Y. Ahn, and J. H. Park. 2011. Effectiveness of slow freezing and vitrification for long-term preservation of mouse ovarian tissue. Theriogenology 75: 1045-1051. Koseoglu, M., A. Evoglu, M. Toner, and K. C. Sadler. 2001. Starfish oocytes form intracellular ice formation at unusually high temperatures. Cryobiology 43: 248-259. Leibo, S. P., J. J. McGrath, and E. G. Cravalho. 1978. Microscopic observation of intracellular ice formation in unfertilized mouse ova as a function of cooling rate. Cryobiology 15: 257-271. Lin, T. T. 1992. Stochastic modeling and analysis of intracellular ice formation phenomenon in biological cells. Journal of Agricultural Machinery 4: 10-21. Lin, T. T., F. L. Chen, and N. H. Chao. 1995. Characterization and modeling of cryoprotectant permeation to small abalone eggs. Memoirs of the College of Agriculture, National Taiwan University 35: 15-31. Lin, Y.A., M. H. Liao, and M. C. Chen. 2009. Effects of Porcine Follicular Fluid and Hormone Supplements on Oocytes In Vitro Maturation and Developmental Capacity. Bulletin on Hungkuang Institute of Technology: 91-99. Magistrini, M., and D. Szöllösi. 1980. Effects of cold and of isopropyl-N- phenylcarbamate on the second meiotic spindle of mouse oocytes. Eur. J. Cell Biol. 22(2):699-707. Martinez, A. G., and M. Matkovic. 1998. Cryopreservation of ovine embryos: slow freezing and vitrification. Theriogenology 49: 1039-1049. Mazur, P. 1963. Kinetics of water loss from cells at subzero temperatures and the likelihood of intracellular freezing. J. Gen. Physiol. 47: 347-369. Mazur, P. 1977. Slow-freezing injury in mammalian cells. In: The freezing of mammalian embryos. Ciba Foundation Symp. K. Elliot. And J. Whelan, editors 52: 19-48. Mazur, P. 1984. Freezing of living cells: mechanism and implications. Am. J. Physiol 247: 125-147. Mikula, A., T. Tykarska, and M. Kuras. 2005. Ultrasturucyure of Gentiana tibetica proembryogentic cells before and after cooling treatment. CryoLetters 26(6): 367-378. Miller, R. H., and P. Mazur. 1976. Survival of frozen-thawed human red cells as function of cooling and warming velocities. Cryobiology 13: 404-414. Miller, I., and J. E. Freund. 1965. Probability and statistics for engineers. Prentice-Hall, Englewood Cliffs, NJ: 432. Mohr L. R. & Trounson AO. 1980. The use of fluorescein diacetate to assess embryo viability in the mouse. J. Reprod. Fertil. 58, 189-196. Muldrew, K., and L. E. McGann. 1994. The osmotic rupture hypothesis of intracellular freezing injury. Biophys. J. 66: 532-541. Myers, S. P., T. T. Lin, R. E. Pitt, and P. L. Steponkus. 1987. Cryobehavior of immature bovine oocyte. CryoLetters: 260-275. Otoi, T., S. Tachikawa, S. Kondo, and T. Suzuki. 1992. Development capacity of bovine oocytes cryopreserved after maturation in vitro and of frozen-thawed bovine embryo derived from frozen mature oocytes. Theriogenology 38: 711-719. Otoi, T., K. Yamamoto, N. Koyama, and T. Suzuki. 1995. In vitro fertilization and development of immature and mature bovine oocytes cryopreserved by ethylene glycol with sucrose. Cryobiology 32: 455-460. Paniagua-Chavez, C. G., J. Jenkins, M. Segovia, and T. R. Tiersch. 2006. Assessment of gamete quality for the eastern oyster (Crassostrea virginica) by use of fluorescent dyes. Cryobiology 53: 128-138. Paredes, E., and J. Bellas. 2009. Cryopreservation of sea urchin embryos (Paracentrotus lividus) applied to marine ecotoxicological studies. Cryobiology 59: 344-350. Parks, J. E., and N. A. Fuffing. 1992. Factors affecting low temperature survival of mammalian oocytes. Theriogenology 37: 59-73. Poncet, J. M., A. Serpentini, E. Boucaud-Camou, and J. M. Lebel. 2002. Cryopreservation of mantle dissociated cells from Haliotis tuberculata (Gastropoda) and postthawed primary cell cultures. Cryobiology 44: 38-45. Pitt, R. E. and P. L. Steponkus. 1989. Quantitative analysis of the probability of intracellular ice formation during freezing of isolated protoplasts. Cryobiology 26(1): 44-63. Pitt, R. E., S. P. Myers, T. T. Lin, and P. L. Steponkus. 1991. Subfreezing volumetric behavior and stochastic modeling of intracellular ice formation in Drosophila melanogaster embryos. Cryobiology 28: 72-86. Porcu, E., R. Fabbri, G. Damiano, S. Giunchi, R. Fratto, and P. M. Ciotti. 2000. Clinical experience and applications of oocyte cryopreservation. Mol. Cell Endocrinol. 169: 33-37. Prentice, J. R., and M. Anzar. 2011. Cryopreservation of mammalian oocyte for conservation of animal genetics. Vet. Med. Int.: 1-11. Rall, W. F., and G. M. Faby. 1985. Ice-Free cryopreservation of mouse embryos at -196°C by vitrification. Nature 313: 573-575. Rojas, C., M. J. Palomo, J. L. Albarracin, and T. Mogas. 2004. Vitrification of immature and in vitro matured pig oocytes: study of distribution of chromosomes, microtubules, and actin microfilaments. Cryobiology 49: 211-220. Rubinsky, B., and M. Ikeda. 1985. A cryomicroscope using directional solidification for the controlled freezing of biological material. Cryobiology 22:55-68. Ross-Rodriguez, L. U., J. A. W. Elliott, and L. E. McGann. 2010. Characterization of cryobiological responses in TF-1 cells using interrupted freezing procedures. Cryobiology 60(2): 106-116 Ruffing, N. A., P. L. Steponkus, R. E. Pitt, and J. E. Parks. 1993. Osmometric behavior, hydraulic conductivity, and incidence of intracellular ice formation in bovine oocytes at difference developmental stages. Cryobiology 30: 562-580. Scheiwe, M. W. and C. Körber. 1981. Thermally defined cryomicroscopy and some applications on human leucocytes. J. .Microsc.126:29-44. Schiewe, M. C., W. F. Rall, L. D. Stuart, and D. E. Wildt. 1991. Analysis of cryoprotectant, cooling rate and in situ dilution using conventional freezing or vitrification for cryopreserving sheep embryos. Theriogenology 36:279-293. Steponkus, P. L., M. F. Dowgert, J. R. Ferguson, and R. L. Levin. 1984. Cryomicroscopy of isolated plant protoplast. Cryobiology 21: 209-233. Sui, L., R. Nomura, Y. Dong, F. Yamaguchi, K. Izumori, and M. Tokuda. 2007. Cryoprotective effects of D-allose on mammalian cells. Cryobiology 55: 87-92. Suzuki, T., A. Boediono, M. Takagi, S. Saha, and C. Sumantri. 1996. Fertilization and Development of frozen–thawed germinal vesicle bovine oocytes by a one-step dilution methodin vitro. Cryobiology 33: 515-524 Takahashi, Y., M. J. Rabins ,and D. M. Auslander. 1970. Control and dynamic systems. reading. Massachusetts: Addison-Wesley. Tarkowski, A. K. 1966. An air-drying method for chromosome preparations from mouse eggs. Cytogenetics 5: 394-400. Toner, M., and E. G. Cravalho. 1990. Thermodynamics and kinetics of intracellular ice formation during freezing of biological cells. J. Appl. Phys. 67(3): 1582-1593. Tsai, H. P., and N. H. Chao. 1994. Cryopreservation of small abalone (Haliotis diversicolor) sperm─technique and its significance. J. Fish. Soc. Taiwan 21: 347-360. Wang, W. H., L. R. Abeydeera, T. C. Cantley, and B. N. Day. 1997. Effects of oocyte maturation media on development of pig embryos produced by in vitro fertilization. J. Repord. Fert. 111: 101-108. Whittingham, D. G. 1972. Survival od mouse embryos frozen to -196°C and -269°C. J. Sci. 178: 411. Wu, C. H. 2005. Improvement of a TEC Cryomicroscope and Freezing Experiments on Zebrafish Embryos. Thesis. National Taiwan University, Taiwan. Wu, M. C., and H. M. Lee. 1996. Vitrification of porcine oocytes. J Chin. Soc. Anim. Sci. 25: 35-51. Wu, T. L. 2007. Modular Design of a TEC Cryomicroscope System and Freezing Experiments of Microcapsules. Thesis. National Taiwan University, Taiwan. Zhou, G. B., and N. Li. 2009. Cryopreservation of porcine oocytes: recent advances . Mol. Hum. Reprod. 15(5): 279-285 Ziegler, J. G., N. B. Nichols, and N. Y. Rochester. 1942. Optimum Settings for Automatic Controllers. Trans. Am. Soc. Mech. Eng. 64: 759-768. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63959 | - |
| dc.description.abstract | 本研究之目的為研製溫度控制準確且易操作的熱電致冷低溫顯微鏡系統，並將其應用於九孔卵及原泡核期(germinal vesicle，GV)豬卵母細胞低溫凍結特性的研究及分析。熱電致冷低溫顯微鏡性能測試的結果為：在恒溫控制方面，溫度最低可達溫-55degreeC，最高溫可達70degreeC，恒溫溫度可在此範圍內任意設定。至於降溫速率方面最快為70degreeC/min；升溫速率最快為112degreeC/min。實驗所記錄到的溫度值與設定值之均方誤差均在0.4degreeC之內。九孔卵之細胞內凍結 (intracellular ice formation, IIF)實驗結果發現：在二甲基亞碸(dimethyl sulfoxide, DMSO)的濃度為2.0、2.5、3.0和4.0M等以及降溫速率為1.5、3、7和12degreeC/min等範圍內，九孔卵發生細胞內凍結的機率可以獲得有效的抑制。為降低溶液效應(solution toxicity effect, STE)的影響，選擇2.0M DMSO為懸浮溶液，並以1.5degreeC/min之降溫速率對九孔卵進行細胞膜滲透活性的觀察，其本研究之目的為研製溫度控制準確且易操作的熱電致冷低溫顯微鏡系統，並將其應用於九孔卵及原泡核期(germinal vesicle，GV)豬卵母細胞低溫凍結特性的研究及分析。熱電致冷低溫顯微鏡性能測試的結果為：在恒溫控制方面，溫度最低可達溫-55degreeC，最高溫可達70degreeC，恒溫溫度可在此範圍內任意設定。至於降溫速率方面最快為70degreeC/min；升溫速率最快為112degreeC/min。實驗所記錄到的溫度值與設定值之均方誤差均在0.4degreeC之內。九孔卵之細胞內凍結 (intracellular ice formation, IIF)實驗結果發現：在二甲基亞碸(dimethyl sulfoxide, DMSO)的濃度為2.0、2.5、3.0和4.0M等以及降溫速率為1.5、3、7和12degreeC/min等範圍內，九孔卵發生細胞內凍結的機率可以獲得有效的抑制。為降低溶液效應(solution toxicity effect, STE)的影響，選擇2.0M DMSO為懸浮溶液，並以1.5degreeC/min之降溫速率對九孔卵進行細胞膜滲透活性的觀察，其結果為累積細胞內凍結機率為15.8%，解凍後有48.8%的九孔卵可以恢復其細胞膜的滲透活性，當降溫速率加快，則恢復活性的比例減少。以相同條件進行冷凍保存卵解凍後的受精實驗，正規化存活率為23.7%(發育為浮游)。至於豬卵母細胞，本研究用24、12、6、3、及1.5degreeC/min的降溫速率，成熟培養液(NCSU-23)混合2.0M DMSO為懸浮溶液，從20degreeC等速率降溫至-50degreeC對GV期豬卵母細胞進行IIF實驗，發現各降溫速率對應的累積細胞內凍結機率分別為100%、100%，50.5%、54.3%及58.6%。GV期豬卵母細胞解凍後經44小時的體外成熟培養，以Hoechst 33258染色法進行檢驗，如果發育到第二次減數分裂(metaphase II)期則判定為存活。6、3、及1.5degreeC/min等降溫速率分別對應的正規化存活率為37.7+-4.6%, 45.0+-4.4%以及 45.4+-5.9%。實驗結果顯示傳統的低溫冷凍保存法可以成功地冷凍保存九孔卵及豬卵母細胞，本研究並提出應用電熱致冷低溫顯微鏡探討九孔卵及豬卵母細胞之低溫凍結特性以選取最佳冷凍條件的方法。 | zh_TW |
| dc.description.abstract | This study aimed to develop a cryomicroscope system based on thermoelectric cooling (TEC) and to use the TEC cryomicroscope system to investigate intracellular ice formation (IIF) behaviors of small abalone eggs and germinal vesicle (GV) stage porcine oocytes. The TEC cryomicroscope system can achieve accurate control of cooling rate up to 70degreeC/min and heating rate up to 112degreeC/min. The isothermal control can be achieved between 70degreeC and -55degreeC with root-mean-squared-error less than 0.4degreeC. According to the IIF experiments of small abalone eggs, IIF were well suppressed at the cooling rates of 1.5, 3, 7, 12degreeC/min in the presence of 2.0, 2.5, 3.0 and 4.0 M dimethyl sulfoxide (DMSO), respectively. Thus, 2.0 M DMSO was selected to be the suspension solution for considering the solution toxicity effect (STE)，and the eggs were cooled at 1.5 degreeC/min to perform osmotic observation. The results showed 48.8% of small abalone eggs was osmotically active after thawing and higher freezing rate reduced the percentage of osmotically active eggs. By separate experiment of fertility test with 1.5degreeC/min cooling rate in the presence of 2.0 M DMSO as cryopreservation protocol, a normalized hatching rate of 23.7% was obtained. As to IIF experiments of germinal vesicle (GV) stage porcine oocytes , five constant cooling rates of 24, 12, 6, 3 and 1.5degreeC/min were tested in experiments in freezing GV porcine oocytes from 20degreeC to -50degreeC in an NCSU-23 medium plus 2.0 M DMSO. The total cumulative probabilities of IIF temperature distribution were 100%, 100%, 50.0%, 54.3% and 58.6% at cooling rates of 24, 12, 6, 3 and 1.5degreeC/min, respectively. Further experiments were performed to examine the feasibility of using these protocols to cryopreserve GV porcine oocytes. After 44 h of in-vitro maturation in NCSU-23, the survival of thawed oocytes was checked. The survival was defined as porcine oocytes developed from the GV stage to the MII stage by using Hoechst 33258 staining. Normalized survival rates of 37.7+-4.6%, 45.0+-4.4% and 45.4+-5.9% were obtained for GV oocytes frozen at 1.5, 3 and 6degreeC/min, respectively. The experimental results indicate that slow freezing is a feasible approach for cryopreservation of small abalone eggs and GV porcine oocytes when cooling rate is properly selected. This study also demonstrated an efficient approach for investigating optimal cooling rates by assessing the IIF characteristics of small abalone eggs and GV porcine COCs by using a TEC cryomicroscope system. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T17:24:27Z (GMT). No. of bitstreams: 1 ntu-101-D93631004-1.pdf: 4401491 bytes, checksum: db3fbc25aef3b528bd44b413ca16d76c (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 誌謝 ……………………………………………………………iii
摘要 ……………………………………………………………v ABSTRACT ..…………………………………………………..vi LIST OF TABLES ……………………………………………xii LIST OF FIGURES ………………………………………….xiii CHAPTER 1 INTRODUCTION ………………...…..….…….1 1.1 Background and Motivation …………………………………...…2 1.1.1 Small abalone eggs …………………………………………………….2 1.1.2 Germinal vesicle porcine oocytes ……………………………………..2 1.2 Objectives ………………………………………………………….4 CHAPTER 2 LITERATURE REVIEW ………...……………6 2.1 Cryomicroscope system …………………………………..……….8 2.2 Intracellular ice formation and cryopreservation ………………13 2.2.1 Thermodynamic considerations of ice formation ………………….13 2.2.1.1 Critical radius of ice formation ……………………….13 2.2.1.2 Homogeneous nucleation ………………………………15 2.2.1.3 Heterogeneous nucleation ……………………………..16 2.2.1.4 Vitrification …………………………………………….17 2.2.2 Cryopreservation of small abalone and porcine …………………..17 2.2.2.1 Cryopreservation of small abalone …………………..20 2.2.2.2 Cryopreservation of GV porcine oocyte ..……………..21 2.3 Stochastic model of intracellular ice formation …………………24 CHAPTER 3 MATERIALS AND METHOD ……...…….…27 3.1 Development of a TEC cryomicroscope system ……………..…27 3.2 Haliotis diversicolor (Small abalone) …………………………...30 3.2.1 Small abalone eggs and its fertilization …………………………...33 3.2.2 Experiments on small abalone egg IIF characterization …………36 3.2.3 Fertility test …………………………………………………………38 3.3 Germinal vesicle porcine oocyte (GV porcine oocyte) ………...39 3.3.1 Sample preparation …………………………………………………39 3.3.2 Medium and cryoprotectant ………………………………………..40 3.3.3 Experiments on GV porcine oocyte IIF characterization ………...43 3.3.4 Survival assay of oocytes ……………………………………………46 3.4 Statistical analysis ………………………………………………..46 CHAPTER 4 RESULTS …...…………………………………48 4.1 Program for integration of a TEC cryomicroscope system by using LabVIEW development kit ……………………………...48 4.1.1 LabVIEW development kit ………………………………………48 4.1.2 The monitor window of the TEC cryomicroscope system ……..49 4.1.3 The block diagrams of the TEC cryomicroscope system ………50 4.1.3.1 Temperature control …………………………...……50 4.1.3.2 Image processing …………………………………….55 4.1.4 Temperature control employed PID algorithm …………………58 4.2 Cryopreservation of small abalone eggs based on IIF assessment ………………………………………………………………….…63 4.2.1 Observations of IIF behavior of small abalone eggs ……………63 4.2.2 Cumulative probability of IIF temperature distribution ………65 4.2.3 Observations of osmotical activity of small abalone eggs after cooling and thawing processes …………………………………..75 4.2.4 Fertility test …………………………………………………….…79 4.3 Cryopreservstion of GV porcine oocytes based on IIF assessment ……………………………………………………..83 4.3.1 Observations of IIF behavior of GV porcine oocytes …………..83 4.3.2 Cumulative probability of IIF temperature distribution ……….85 4.3.3 Survival of GV porcine oocytes that underwent a freeze-thaw process ……………………………………………………………..86 CHAPTER 5 DISCUSSION …..……………………………...91 5.1 TEC cryomicroscope system…………………………………...91 5.2 Characterization of intracellular ice formation behavior of small abalone eggs …………………………………………….94 5.2.1 Fitting with Weibull probabilistic model ………………………..94 5.2.2 IIF behavior as affected by cooling rate and concentration of DMSO ……………………………………………………………..99 5.2.3 cryoinjury of small abalone eggs………………………………..104 5.3 Characterization of intracellular ice formation behavior of GV porcine oocytes ………………………………………………105 5.3.1 Analysis of the cumulative probabilities of IIF temperature distribution ……………………………………………………….105 5.3.2 Fitting with Weibull probabilistic model ……………….………108 5.3.3 Survival as affected by cooling rate …………………………….113 CHAPTER 6 CONCLUSION AND SUGGESTION …...…115 6.1 Conclusion………………………………………………………115 6.1.1 TEC cryomicroscope system……………………………………115 6.1.2 Small abalone eggs………………………………………………116 6.2.3 GV porcine oocytes……………………………………………...119 6.2 Suggestion………………………………………………………121 REFERENCE……………………………………..……….…123 中文節要....................................................................................131 | |
| dc.language.iso | en | |
| dc.subject | 細胞內凍結 | zh_TW |
| dc.subject | 九孔 | zh_TW |
| dc.subject | 豬 | zh_TW |
| dc.subject | 冷凍保存 | zh_TW |
| dc.subject | TEC低溫顯微鏡 | zh_TW |
| dc.subject | Cryopreservation | en |
| dc.subject | Abalone | en |
| dc.subject | IIF | en |
| dc.subject | Porcine | en |
| dc.subject | TEC cryomicroscope | en |
| dc.title | 應用熱電致冷低溫顯微鏡探討九孔卵及豬卵母細胞之低溫凍結特性 | zh_TW |
| dc.title | Study on Characteristics of Intracellular Ice Formation Behavior for Haliotis Diversicolor Eggs and Porcine Oocytes by a Thermal Electric Cooled Cryomicroscope | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 江昭皚(Joe-Air Jiang),雷鵬魁(Perng-Kwei Lei),吳柏青(Po-Ching Wu),陳銘正(Ming-Cheng Chem) | |
| dc.subject.keyword | 細胞內凍結,九孔,豬,冷凍保存,TEC低溫顯微鏡, | zh_TW |
| dc.subject.keyword | IIF,Abalone,Porcine,Cryopreservation,TEC cryomicroscope, | en |
| dc.relation.page | 150 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-08-16 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
| 顯示於系所單位： | 生物機電工程學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-101-1.pdf 未授權公開取用 | 4.3 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。