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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 賈景山 | |
dc.contributor.author | Yu-Hsuan Lin | en |
dc.contributor.author | 林昱璇 | zh_TW |
dc.date.accessioned | 2021-06-16T17:22:40Z | - |
dc.date.available | 2017-09-19 | |
dc.date.copyright | 2012-09-19 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-16 | |
dc.identifier.citation | 1 Ko, Y. C. et al. Betel Quid Chewing, Cigarette-Smoking and Alcohol-Consumption Related to Oral-Cancer in Taiwan. J Oral Pathol Med 24, 450-453 (1995).
2 Kreimer, A. R., Clifford, G. M., Boyle, P. & Franceschi, S. Human papillomavirus types in head and neck squamous cell carcinomas worldwide: A systematic review. Cancer Epidem Biomar 14, 467-475 (2005). 3 Andrews, E., Seaman, W. T. & Webster-Cyriaque, J. Oropharyngeal carcinoma in non-smokers and non-drinkers: A role for HPV. Oral Oncol 45, 486-491 (2009). 4 Rodriguez-Caballero, A. et al. Cancer treatment-induced oral mucositis: a critical review. Int J Oral Max Surg 41, 225-238 (2012). 5 Sonis, S. T. et al. Perspectives on cancer therapy-induced mucosal injury - Pathogenesis, measurement, epidemiology, and consequences for patients. Cancer 100, 1995-2025 (2004). 6 Burdelya, L. G. et al. Toll-like receptor 5 agonist protects mice from dermatitis and oral mucositis caused by local radiation: implications for head-and-neck cancer radiotherapy. International journal of radiation oncology, biology, physics 83, 228-234 (2012). 7 Nonzee, N. J. et al. Evaluating the supportive care costs of severe radiochemotherapy-induced mucositis and pharyngitis - Results from a Northwestern University costs of cancer program pilot study with head and neck and nonsmall cell lung cancer patients who received care at a county hospital, a Veterans Administration Hospital, or a comprehensive cancer care center. Cancer 113, 1446-1452 (2008). 8 Sonis, S. T. The pathobiology of mucositis. Nature reviews. Cancer 4, 277-284 (2004). 9 Sonis, S. et al. Gene expression changes in peripheral blood cells provide insight into the biological mechanisms associated with regimen-related toxicities in patients being treated for head and neck cancers. Oral Oncol 43, 289-300 (2007). 10 Sonis, S. T. Oral mucositis. Anti-Cancer Drug 22, 607-612 (2011). 11 Sonis, S. T. Mucositis: The impact, biology and therapeutic opportunities of oral mucositis. Oral Oncol 45, 1015-1020 (2009). 12 Sonis, S. T. Pathobiology of oral mucositis: novel insights and opportunities. The journal of supportive oncology 5, 3-11 (2007). 13 Le, Q. T. et al. Palifermin reduces severe mucositis in definitive chemoradiotherapy of locally advanced head and neck cancer: a randomized, placebo-controlled study. Journal of clinical oncology : official journal of the American Society of Clinical Oncology 29 (2011). 14 Henke, M. et al. Palifermin decreases severe oral mucositis of patients undergoing postoperative radiochemotherapy for head and neck cancer: a randomized, placebo-controlled trial. Journal of clinical oncology : official journal of the American Society of Clinical Oncology 29, 2815-2820 (2011). 15 Saha, S. et al. TLR9 Agonist Protects Mice from Radiation-Induced Gastrointestinal Syndrome. Plos One 7, e29357 (2012). 16 Grdina, D. J. et al. Amifostine induces antioxidant enzymatic activities in normal tissues and a transplantable tumor that can affect radiation response. International journal of radiation oncology, biology, physics 73, 886-89 (2009). 17 Brizel, D. M. et al. Phase III randomized trial of amifostine as a radioprotector in head and neck cancer. Journal of Clinical Oncology 18, 3339-3345 (2000). 18 Brizel, D. M. Pharmacologic approaches to radiation protection. Journal of Clinical Oncology 25, 4084-4089 (2007). 19 Hudes, G. R. et al. NCCN Task Force report: optimizing treatment of advanced renal cell carcinoma with molecular targeted therapy. Journal of the National Comprehensive Cancer Network : JNCCN 9 Suppl 1, S1-29 (2011). 20 Murphy, C. K. et al. Efficacy of superoxide dismutase mimetic M40403 in attenuating radiation-induced oral mucositis in hamsters. Clinical cancer research : an official journal of the American Association for Cancer Research 14, 4292-4297 (2008). 21 Keefe, D. M., Sonis, S. T. & Bowen, J. M. Emerging drugs for chemotherapy-induced mucositis. Expert opinion on emerging drugs 13, 511-522 (2008). 22 Keefe, D. M., Sonis, S. T. & Bowen, J. M. Emerging drugs for chemotherapy-induced mucositis. Expert Opin Emerg Dr 13, 511-522 (2008). 23 Watkins, B., Pouliot, K., Fey, E., Tuthill, C. & Sonis, S. Attenuation of radiation- and chemoradiation-induced mucositis using gamma-D-glutamyl-L-tryptophan (SCV-07). Oral diseases 16, 655-660 (2010). 24 Caluwaerts, S. et al. AG013, a mouth rinse formulation of Lactococcus lactis secreting human Trefoil Factor 1, provides a safe and efficacious therapeutic tool for treating oral mucositis. Oral Oncol 46, 564-570 (2010). 25 Chang, T. T. & Chou, W. N. Antrodia-Cinnamomea Sp-Nov on Cinnamomum-Kanehirai in Taiwan. Mycol Res 99, 756-758 (1995). 26 Wu, S. H., Ryvarden, L. & Chang, T. T. Antrodia camphorata ('niu-chang-chih'), new combination of a medicinal fungus in Taiwan. Bot Bull Acad Sinica 38, 273-275 (1997). 27 Geethangili, M. & Tzeng, Y. M. Review of Pharmacological Effects of Antrodia camphorata and Its Bioactive Compounds. Evidence-based complementary and alternative medicine : eCAM 2011, 212641 (2011). 28 Shen, Y. C. et al. Anti-inflammatory activity of the extracts from mycelia of Antrodia camphorata cultured with water-soluble fractions from five different Cinnamomum species. Fems Microbiol Lett 231, 137-143 (2004). 29 Hseu, Y. C. et al. Anti-inflammatory potential of Antrodia Camphorata through inhibition of NOS, COX-2 and cytokines via the NF-kappa B pathway. Int Immunopharmacol 5, 1914-1925 (2005). 30 Rao, Y. K., Fang, S. H. & Tzeng, Y. M. Evaluation of the anti-inflammatory and anti-proliferation tumoral cells activities of Antrodia camphorata, Cordyceps sinensis, and Cinnamomum osmophloeum bark extracts. Journal of ethnopharmacology 114, 78-85 (2007). 31 Wen, C. L. et al. Anti-inflammatory effects of methanol extract of Antrodia cinnamomea mycelia both in vitro and in vivo. Journal of ethnopharmacology 137, 575-584 (2011). 32 Chang, J. M. et al. An Extract of Antrodia camphorata Mycelia Attenuates the Progression of Nephritis in Systemic Lupus Erythematosus-Prone NZB/W F1 Mice. Evidence-based complementary and alternative medicine : eCAM 2011, 465894 (2011). 33 Song, T. Y. & Yen, G. C. Antioxidant properties of Antrodia camphorata in submerged culture. Journal of agricultural and food chemistry 50, 3322-3327 (2002). 34 Hseu, Y. C., Chen, S. C., Yech, Y. J., Wang, L. & Yang, H. L. Antioxidant activity of Antrodia camphorata on free radical-induced endothelial cell damage. Journal of ethnopharmacology 118, 237-245 (2008). 35 Yang, H. L. et al. Antrodia camphorata in submerged culture protects low density lipoproteins against oxidative modification. The American journal of Chinese medicine 34, 217-231 (2006). 36 Wu, X. Y. et al. A Novel Peptide to Treat Oral Mucositis Blocks Endothelial and Epithelial Cell Apoptosis. Int J Radiat Oncol 83, E409-E415 (2012). 37 Murray, L. A. et al. Serum amyloid P ameliorates radiation-induced oral mucositis and fibrosis. Fibrogenesis & tissue repair 3, 11 (2010). 38 Cotrim, A. P. et al. Pharmacological protection from radiation +/- cisplatin-induced oral mucositis. International journal of radiation oncology, biology, physics 83, 1284-1290 (2012). 39 Zheng, C. Y. et al. Prevention of Radiation-Induced Oral Mucositis after Adenoviral Vector - Mediated Transfer of the Keratinocyte Growth Factor cDNA to Mouse Submandibular Glands. Clinical Cancer Research 15, 4641-4648 (2009). 40 Jaal, J., Richter, C. & Dorr, W. Effect of recombinant human keratinocyte growth factor (delta 23rHuKGF, Palifermin) on inflammatory and immune changes in mouse tongue during fractionated irradiation. Int J Radiat Biol 86, 860-866 (2010). 41 Ryu, S. H. et al. Therapeutic Effects of Recombinant Human Epidermal Growth Factor (rhEGF) in a Murine Model of Concurrent Chemo- and Radiotherapy-Induced Oral Mucositis. J Radiat Res 51, 595-601 (2010). 42 Chen, P. L., Lingen, M., Sonis, S. T., Walsh-Reitz, M. M. & Toback, F. G. Role of AMP-18 in oral mucositis. Oral Oncol 47, 831-839 (2011). 43 Zhao, J. et al. R-Spondin1 protects mice from chemotherapy or radiation-induced oral mucositis through the canonical Wnt/beta-catenin pathway. Proceedings of the National Academy of Sciences of the United States of America 106, 2331-2336 (2009). 44 Yeoh, A. S. et al. A novel animal model to investigate fractionated radiotherapy-induced alimentary mucositis: the role of apoptosis, p53, nuclear factor-kappaB, COX-1, and COX-2. Molecular cancer therapeutics 6, 2319-2327 (2007). 45 Ong, Z. Y. et al. Pro-inflammatory cytokines play a key role in the development of radiotherapy-induced gastrointestinal mucositis. Radiat Oncol 5, 22 (2010). 46 Citrin, D. et al. Radioprotectors and Mitigators of Radiation-Induced Normal Tissue Injury. Oncologist 15, 360-371 (2010). 47 Scholzen, T. & Gerdes, J. The Ki-67 protein: From the known and the unknown. J Cell Physiol 182, 311-322 (2000). 48 Liu, D. Z. et al. Comparative anti-inflammatory characterization of wild fruiting body, liquid-state fermentation, and solid-state culture of Taiwanofungus camphoratus in microglia and the mechanism of its action. Journal of ethnopharmacology 113, 45-53 (2007). 49 Mitchell, J. B. et al. Inhibition of Oxygen-Dependent Radiation-Induced Damage by the Nitroxide Superoxide-Dismutase Mimic, Tempol. Arch Biochem Biophys 289, 62-70 (1991). 50 Cotrim, A. P. et al. Differential radiation protection of salivary glands versus tumor by tempol with accompanying tissue assessment of tempol by magnetic resonance Imaging. Clinical Cancer Research 13, 4928-4933 (2007). 51 Hahn, S. M. et al. Evaluation of tempol radioprotection in a murine tumor model. Free Radical Bio Med 22, 1211-1216 (1997). 52 Metz, J. M. et al. A phase I study of topical tempol for the prevention of alopecia induced by whole brain radiotherapy. Clinical Cancer Research 10, 6411-6417 (2004). 53 Hahn, S. M. et al. Tempol, a Stable Free-Radical, Is a Novel Murine Radiation Protector. Cancer Res 52, 1750-1753 (1992). 54 Irita, J. et al. Osteopontin deficiency protects against aldosterone-induced inflammation, oxidative stress, and interstitial fibrosis in the kidney. Am J Physiol-Renal 301, F833-F844 (2011). 55 Chen, G. et al. Rapamycin ameliorates kidney fibrosis by inhibiting the activation of mTOR signaling in interstitial macrophages and myofibroblasts. Plos One 7, e33626 (2012). 56 Yang, Y. H. et al. Reduction in arthritis severity and modulation of immune function in tissue factor cytoplasmic domain mutant mice. Am J Pathol 164, 109-117 (2004). | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63906 | - |
dc.description.abstract | 絕大多數的頭頸部癌症患者接受放射線治療後會產生副作用—口腔黏膜炎(oral mucositis),其發生率高達八至九成。口腔黏膜炎指的是口腔黏膜紅腫、萎縮、潰瘍等現象,這會造成患者進食、吞嚥、說話等方面的困難,不但影響生活品質,還會使患者產生劇烈的疼痛感,甚至導致治療計畫中斷,增加患者於術後照護上的經濟負擔以及傷口持續感染的可能性,然而並沒有有效的藥物來治療或預防口腔粘膜炎。牛樟芝Antrodia Camphorata為台灣特有的真菌,生長在海拔200~2000公尺以上的山中,且只長於牛樟樹,取得十分不易。許多文獻指出牛樟芝具有抗癌的效果,此外也具有抗發炎、抗氧化以及免疫調節的能力,本實驗即在評估牛樟芝是否可以緩解放射線所引發的口腔黏膜炎。實驗以舌頭組織作為觀察對象,於照射放射線前五天開始每天餵食牛樟芝或水,再以分次劑量5 x 10 Gy或單次劑量25 Gy放射線照射B6小鼠口吻部誘發口腔黏膜炎,並於照射後不同時間點犧牲小鼠取下舌部組織。兩種劑量下皆可成功引發小鼠舌頭潰瘍,但5 x 10 Gy模式小鼠死亡率極高,僅能觀察急性期之變化;25 Gy模式則能觀察到體重、舌頭外觀、組織學及發炎反應在急性期與復原期之變化。此外,實驗結果發現恢復期時舌尖恢復速度較舌中快,而急性期時餵食牛樟芝小鼠之IL-1βmRNA表現量低於餵食水的老鼠。 | zh_TW |
dc.description.abstract | Oral mucositis is a common side effect of radiotherapy of patients with head-and-neck cancer. Persistent swelling, ulceration and atrophy of oral mucosa causes extreme discomfort, affects nutritional intake, and reduces patient quality of life. In addition, it increases the use of health resources, leads to oral and systemic infections as well as interrupts therapy. There are a lot of treatments for oral mucositis; unfortunately, the recommend treatment seems palliative. Antrodia camphorate (AC), a well-known Chinese folk medicine, is a fungal parasite on the inner cavity of the endemic species Cinnamomum kanehirae which only grows at an altitude of 200~2000 m in Taiwan. Many studies reveal that AC possesses extensive pharmacological effects, such as anti-cancer, anti-oxidant, immunomodulatory and anti-inflammatory activities. Hence, our specific aim is to evaluate whether AC can ameliorate radiation-induced oral mucositis. The changes of appearance, histology and immunology in both acute and recovery phase indicated we successfully established an oral mucositis mouse model by 25 Gy irradiation dose. Furthermore, our data suggested the tongue tip recovered more quickly than the tongue middle in recovery phase, and AC fed mice expressed lower IL-1βmRNA level than control mice in acute phase. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T17:22:40Z (GMT). No. of bitstreams: 1 ntu-101-R99450018-1.pdf: 4594148 bytes, checksum: 5fc9895defb7408d3a2a323e111340f2 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii 英文摘要 iii 目錄 iv 圖表目錄 vi 第一章 緒論 1 1.1 口腔癌 1 1.2 口腔黏膜炎 2 1.3 牛樟芝 6 1.4 放射線引起之併發症之動物模式簡介 9 1.5 本實驗之設計與目標 10 第二章 實驗材料與方法 11 2.1 實驗動物及分組 11 2.2 牛樟芝製備及灌食 11 2.3 TEMPOL製備及施打 12 2.4 放射線照射 12 2.5 口腔黏膜炎之觀察與檢體收集 12 2.6 H&E stain 13 2.7 Toluidine blue stain 13 2.8 免疫組織化學染色(Immunohistochemistry stain) 14 2.9 組織切片圖定量分析 15 2.10 組織核糖核酸萃取(tissue RNA extraction) 16 2.11 訊息核糖核酸之反轉錄(mRNA reverse transcription) 16 2.12 即時定量聚合酶連鎖反應(real-time PCR) 17 2.13 血清中Nitrite濃度測定(Griess assay) 17 2.14 統計方法 18 第三章 實驗結果 19 3.1分次劑量5 x 10 Gy照射結果 19 3.1.1 體重 19 3.1.2 口腔黏膜炎表現 20 3.1.3 舌頭組織學變化 20 3.1.4 前發炎性細胞激素相關基因表現量 21 3.2 單次劑量25 Gy照射結果 22 3.2.1 體重 22 3.2.2 口腔黏膜炎表現 23 3.2.3 組織學變化 23 3.2.4 前發炎性細胞激素相關基因表現量 25 3.2.5 血清中nitrite濃度變化 26 3.2.6 單次劑量25 Gy照射後整體變化趨勢 26 3.3 結論 27 第四章 討論 28 4.1 放射線引發口腔黏膜炎之小鼠模式 28 4.2 牛樟芝及TEMPOL效果之探討 29 4.3 麻醉藥之影響 31 參考文獻 32 | |
dc.language.iso | zh-TW | |
dc.title | 放射線引發口腔黏膜潰瘍急性期與復原期之發炎反應 | zh_TW |
dc.title | Inflammatory responses of radiation-induced oral mucositis during acute and recovery stages | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 成佳憲,朱清良 | |
dc.subject.keyword | 口腔黏膜炎,放射線,發炎反應,牛樟芝, | zh_TW |
dc.subject.keyword | oral mucositis,irradiation,inflammatory response,Antrodia camphorata, | en |
dc.relation.page | 63 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-08-16 | |
dc.contributor.author-college | 牙醫專業學院 | zh_TW |
dc.contributor.author-dept | 口腔生物科學研究所 | zh_TW |
顯示於系所單位: | 口腔生物科學研究所 |
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