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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳凱儀(Kai-Yi Chen) | |
| dc.contributor.author | Pei-Wen Yang | en |
| dc.contributor.author | 楊珮雯 | zh_TW |
| dc.date.accessioned | 2021-06-16T17:16:32Z | - |
| dc.date.available | 2012-08-22 | |
| dc.date.copyright | 2012-08-22 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-17 | |
| dc.identifier.citation | 王聖善 (2009) 碩士論文 控制水稻內外穎發育SLP基因座之高解析度遺傳定位與候選基因鑑定。國立臺灣大學農藝研究所。臺北，臺灣，中華民國。
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Proceedings of the Crop Science Society of Japan 45:270–278 Saito K, Hayano-Saito Y, Kuroki M, Sato Y (2010) Map-based cloning of the rice cold tolerance gene Ctb1. Plant Science 179:97-102 Seguro K, Tamiya T, Tsuchiya T, Matsumoto JJ (1989) Effect of chemical modifications on freeze denaturation of lactate dehydrogenase. Cryobiology 26:154–161 Shirasawa S, Endo T, Nakagomi K, Yamaguchi M, Nishio T (2012) Delimitation of a QTL region controlling cold tolerance at booting stage of a cultivar, 'Lijiangxintuanheigu', in rice, Oryza sativa L. Theoretical and Applied Genetics 124:937-946 Suh JP, Jeung JU, Lee JI, Choi YH, Yea JD, Virk PS, Mackill DJ, Jena KK (2010) Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in cold-tolerant genotypes of rice (Oryza sativa L.). Theoretical and Applied Genetics 120:985-995 Suh JP, Lee CK, Lee JH, Kim JJ, Kim SM, Cho YC, Park SH, Shin JC, Kim YG, Jena KK (2012) Identification of quantitative trait loci for seedling cold tolerance using RILs derived from a cross between japonica and tropical japonica rice cultivars. Euphytica 184:101-108 Terashima I, Huang L-K, Osmond CB (1989) Effects of chilling on thylakoid functions, measured at room temperature, in Cucumis sativus L. and Oryza sativa L.. Plant and Cell Physiology 30: 841-850 Thomashow MF (1990) Molecular genetics of cold acclimation in higher plants.Advances in Genetics 28:99–131 Wang ZF, Wang FH, Zhou R, Wang JF, Zhang HS (2011) Identification of quantitative trait loci for cold tolerance during the germination and seedling stages in rice (Oryza sativa L.). Euphytica 181:405-413 Zhang ZH, Su L, Li W, Chen W, Zhu YG (2005) A major QTL conferring cold tolerance at the early seedling stage using recombinant inbred lines of rice (Oryza sativa L.). Plant Science 168:527-534 Zhou L, Zeng Y, Zheng W, Tang B, Yang S, Zhang H, Li J, Li Z (2010) Fine mapping a QTL qCTB7 for cold tolerance at the booting stage on rice chromosome 7 using a near-isogenic line. Theoretical and Applied Genetics 121:895-905 Zhu J, Dong CH, Zhu JK (2007) Interplay between cold-responsive gene regulation, metabolism and RNA processing during plant cold acclimation. Current Opinion in Plant Biology 10: 290-295 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63692 | - |
| dc.description.abstract | 低溫冷害為影響水稻產量的主要因素之一，以在水稻幼苗期與孕穗期所遭受到的傷害最為嚴重。本研究的目的為採用較具耐冷性之稉稻品種臺農67號與不具耐冷性之秈稻品種臺中在來一號所雜交所得之243個重組自交系，進行水稻幼苗耐冷性之數量性狀基因座定位。水稻幼苗的耐冷性分別以幼苗存活率與人為訂定的幼苗耐冷等級測量值表示。研究結果顯示幼苗存活率與幼苗耐冷等級的外表型相關係數達到0.50，兩種外表型所定位到的數量性狀基因座都位於第六條與第十一條染色體上相同的區間，分別與分子標記RC6-1與RC11-11連鎖。幼苗存活率的數量性狀基因座命名為qCTSS6.1與qCTSS 11.1，而幼苗耐冷等級的數量性狀基因座則命名為qCTS6.1與qCTS11.1。幼苗存活率或幼苗耐冷性的兩個數量性狀基因座綜合的效應都能解釋將近30%遺傳定位族群內的外表型變異。 | zh_TW |
| dc.description.abstract | Rice grown in temperate areas is subjected to chilling stress at seedling and booting stages. The purpose of this thesis was to identify quantitative trait loci (QTLs) for chilling tolerance at the seedling stage of rice, using 243 recombinant inbred lines derived from a cross between a chilling-tolerant japonica cultivar, TNG67, and a chilling-sensitive indica cultivar, TN1. The phenotypes of chilling tolreance at seedling stage was scored by the measurements of the survival rate and the artifical chilling-tolerant classes. The results showed that the correlation coefficient between the survival rate and the artifical chilling-tolerant classes was 0.50. Both phenotypic measurements identified two major QTLs on the same chromosomal region: one on chromosome 6, the other on the chromosome 11. The former QTL linked to the SNP marker RC6-1, and the latter QTL linked to the SNP marker RC11-11. The QTLs for the survival rate under chilling stress were named qCTSS6.1 and qCTSS11.1, and the QTLs for the chilling-tolerant classes were named qCTS6.1 and qCTS11.1 The combined effects of the two QTLs were able to explain approximately 30% of phenotypic varions within the genetic mapping population, for either the survival rate or the chilling-tolerant classes. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T17:16:32Z (GMT). No. of bitstreams: 1 ntu-101-R99621123-1.pdf: 1917293 bytes, checksum: fd48b3b5411d549e7dbc22693491e6be (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 目錄
摘要 i Abstract ii 表目錄 iv 圖目錄 v 中英對照表 vi 壹、前人研究 1 一、植物耐冷性 1 二、水稻低溫逆境的生理研究 3 三、水稻耐冷性的遺傳研究 5 四、水稻幼苗耐冷性在臺灣的研究 10 貳、研究目的 12 參、材料方法 13 一、試驗材料 13 二、水稻幼苗期耐冷性檢測 13 三、水稻葉片DNA萃取 14 四、簡單重複序列(SSR)分子標記的基因型鑑定 15 五、單一核苷酸多型性(SNP) 分子標記的設計 16 六、單一核苷酸多型性分子標記的基因型鑑定 18 七、統計分析 19 肆、結果 20 一、外表型檢測結果 20 二、分子標記基因型的檢測結果 20 三、數量性狀基因座分析 21 伍、討論 23 一、低溫處理條件設定 23 二、重組自交系外表型檢測結果 23 三、數量性狀基因座分析 24 四、未來展望 25 引用文獻 26 附錄 45 附件一、卡方適合度檢定分析表 45 附件二、單一標記分析結果 47 附件三、重組自交系基因型資料 49 表目錄 表 一、臺農67號及臺中在來一號兩親本間具多型性之簡單重複序列分子標記的資訊 31 表 二、幼苗期與孕穗期之耐冷性數量性狀基因座相關資訊 33 表 三、臺農67號及臺中在來一號兩親本間具多型性之單一核苷酸多型性分子標記的資訊 34 表 四、重組自交系族群幼苗耐冷性等級之變方分析結果 35 表 五、重組自交系族群幼苗存活率之變方分析結果 36 表 六、重組自交系族群中定位得之幼苗耐冷等級與幼苗存活率數量性狀基因座資訊 37 表 七、利用複回歸分析方法進行分子標記RC6-1與RC11-11對幼苗存活率與幼苗耐冷性總貢獻度分析之所得結果 38 圖目錄 圖 一、遺傳定位族群的建構 39 圖 二、耐冷等級的分級 40 圖 三、臺農67號 (TNG67) 與臺中在來一號 (TN1) 經低溫處理 (5/5˚C) 五天、再回溫 (25/20˚C) 生長八天後，植株恢復生長的情況 41 圖 四、重組自交系族群與臺農67號 (TNG67) 及臺中在來一號 (TN1) 兩親本之 (A) 幼苗存活率分布圖 (B) 幼苗耐冷性等級分布圖 42 圖 五、重組自交系族群幼苗耐冷性等級與幼苗存活率之相關圖 43 圖 六、QTL圖譜 44 | |
| dc.language.iso | zh-TW | |
| dc.subject | 數量性狀基因座 | zh_TW |
| dc.subject | 耐冷性 | zh_TW |
| dc.subject | 水稻 | zh_TW |
| dc.subject | 臺農六十七號 | zh_TW |
| dc.subject | 台中在來一號 | zh_TW |
| dc.subject | Chilling Tolerance | en |
| dc.subject | Rice | en |
| dc.subject | TNG67 | en |
| dc.subject | TN1 | en |
| dc.subject | QTL | en |
| dc.title | 水稻幼苗耐冷性數量性狀基因座之定位 | zh_TW |
| dc.title | QTL Mapping for Chilling Tolerance at Seedling Stage in Rice (Oryza sativa L.) | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 廖振鐸(Chen-Tuo Liao),高景輝(Ching-Huei Kao),胡凱康(Kae-Kang Hwu) | |
| dc.subject.keyword | 數量性狀基因座,耐冷性,水稻,臺農六十七號,台中在來一號, | zh_TW |
| dc.subject.keyword | QTL,Chilling Tolerance,Rice,TNG67,TN1, | en |
| dc.relation.page | 52 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-08-18 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| 顯示於系所單位： | 農藝學系 | |
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