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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鍾嘉綾 | |
dc.contributor.author | Chia-Yi Wu | en |
dc.contributor.author | 吳佳宜 | zh_TW |
dc.date.accessioned | 2021-06-16T17:15:16Z | - |
dc.date.available | 2023-04-15 | |
dc.date.copyright | 2020-04-15 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-04-07 | |
dc.identifier.citation | 李窓明、洪立。1982。草莓栽培品種,溫度,貯藏與果實採收後天數,對種子發芽之影響。中國園藝 28: 36-44。
李窓明。1993。台灣草莓產業演進四十年。農業試驗所特刊 36: 315-332。 李窓明、李聯興、倪萬丁。1993。草莓新品種「桃園二號」育種研究。桃園區農業改良場研究彙報 13: 1-18。 李窓明。2003。草莓本圃栽培管理。桃園區農業專訊 44: 15-20。 吳岱融、盧美君、張廣淼。2019。草苺新品種‘苗栗 1 號’特性之研究。臺灣園藝 65:67-74。 余祥萱。2018。草莓炭疽病菌之毒性代謝物鑑定與功能性分析。國立臺灣大學植物病理與微生物學研究所碩士論文。臺北。臺灣。 胡賢彬。2013。應用黑色素生合成基因研發鑑定炭疽病菌之生物晶片及探討其類緣關係。國立臺灣大學植物病理與微生物學研究所碩士論文。臺北。臺灣。 彭淑貞。2006。草莓炭疽病之發生及防治要領。苗栗區農業專訊 33: 10-11。 彭淑貞、張廣淼。2009。草莓重要病害防治管理。苗栗區農業專訊 48: 5-7。 費雯綺、王喻其。2007。草莓炭疽病。植物保護手冊─果樹篇: 206。 羅國偉、李窓明、張志展。2012。草莓新品種桃園 4 號之育成。桃園區農業改良場研究彙報 72: 1-10。 鐘珮哲、彭淑貞、張廣淼、楊秀珠、余思葳。2012。草莓病蟲害之發生與管理。行政院農業委員會動植物防疫檢疫局和農業藥物毒物試驗所編。 鐘珮哲、彭淑貞。2013。草莓育苗期重要病害管理。苗栗區農業專訊 61:9-10。 鐘珮哲。2016。草莓重要病害檢測及應用。苗栗區農業專訊 73: 13-14。 鐘珮哲、吳竑毅、王彥文、胡賢彬、歐海仁、曾顯雄、鍾嘉綾。2019。臺灣草莓炭疽病菌之族群分析。107年度植病年會論文宣讀。 Adhikari, T., Chacon, J., Fernandez, G., and Louws, F. 2019. First report of anthracnose causing both crown and fruit rot of strawberry by Colletotrichum siamense in North Carolina. Plant Disease 103: 1775-1775. Amil-Ruiz, F., Garrido-Gala, J., Gadea, J., Blanco-Portales, R., Muñoz-Mérida, A., Trelles, O., de los Santos, B., Arroyo, F. T., Aguado-Puig, A., and Romero, F. 2016. Partial activation of SA- and JA-defensive pathways in strawberry upon Colletotrichum acutatum interaction. Frontiers in Plant Science 7: 1-23. Amusa, N. 1998. Evaluation of cassava clones for resistance to anthracnose disease using phytotoxic metabolites of Colletotrichum gloeosporioides f. sp. manihotis and its correlation with field disease reactions. Tropical Agricultural Research and Extension 1: 116-120. Anciro, A., Mangandi, J., Verma, S., Peres, N., Whitaker, V. M., and Lee, S. 2018. FaRCg1: a quantitative trait locus conferring resistance to Colletotrichum crown rot caused by Colletotrichum gloeosporioides in octoploid strawberry. Theoretical and Applied Genetics 131: 2167-2177. Arroyo, F. T., Moreno, J., Daza, P., Boianova, L., and Romero, F. 2007. Antifungal activity of strawberry fruit volatile compounds against Colletotrichum acutatum. Journal of Agricultural and Food Chemistry 55: 5701-5707. Arroyo, F. T., Moreno, J., García-Herdugo, G., Santos, B. D. l., Barrau, C., Porras, M., Blanco, C., and Romero, F. 2005. Ultrastructure of the early stages of Colletotrichum acutatum infection of strawberry tissues. Canadian Journal of Botany 83: 491-500. Baroncelli, R., Zapparata, A., Sarrocco, S., Sukno, S. A., Lane, C. R., Thon, M. R., Vannacci, G., Holub, E., and Sreenivasaprasad, S. 2015. Molecular diversity of anthracnose pathogen populations associated with UK strawberry production suggests multiple introductions of three different Colletotrichum species. PLoS One 10.6 Barritt, B. 1980. Resistance of strawberry clones to Botrytis fruit rot. Journal of the American Society for Horticultural Science 105: 160-164. Bringhurst, R., and Voth, V. 1976. Origin and evolutionary potentiality of day-neutral trait in octoploid Fragaria. Genetics 83: S10-S10. Brooks, A. 1931. Anthracnose of strawberry caused by Colletotrichum fragariae, n. sp. Phytopathology 21: 739-744. Calleja, E., Ilbery, B., Spence, N., and Mills, P. 2013. The effectiveness of phytosanitary controls in preventing the entry of Colletotrichum acutatum in the UK strawberry sector. Plant Pathology 62: 266-278. Cannon, P., Damm, U., Johnston, P., and Weir, B. 2012. Colletotrichum – current status and future directions. Studies in Mycology 73: 181-213. Cantu, D., Vicente, A. R., Labavitch, J. M., Bennett, A. B., and Powell, A. L. 2008. Strangers in the matrix: plant cell walls and pathogen susceptibility. Trends in Plant Science 13: 610-617. Capobiango, N., Pinho, D., Zambolim, L., Pereira, O., and Lopes, U. 2016. Anthracnose on strawberry fruits caused by Colletotrichum siamense in Brazil. Plant Disease 100: 859-859. Casado‐Díaz, A., Encinas‐Villarejo, S., Santos, B. d. l., Schilirò, E., Yubero‐Serrano, E. M., Amil‐Ruíz, F., Pocovi, M. I., Pliego‐Alfaro, F., Dorado, G., and Rey, M. 2006. Analysis of strawberry genes differentially expressed in response to Colletotrichum infection. Physiologia Plantarum 128: 633-650. Chalfoun, N., Castagnaro, A., and Ricci, J. D. 2011. Induced resistance activated by a culture filtrate derived from an avirulent pathogen as a mechanism of biological control of anthracnose in strawberry. Biological Control 58: 319-329. Chandler, C. K., Mertely, J. C., and Peres, N. 2004. Resistance of selected strawberry cultivars to anthracnose fruit rot and Botrytis fruit rot. Pages 123-126 in: V International Strawberry Symposium. Chung, P.-C., Wu, H.-Y., Ariyawansa, H. A., Tzean, S.-S., and Chung, C.-L. 2019. First report of anthracnose crown rot of strawberry caused by Colletotrichum siamense in Taiwan. Plant Disease 103: 1775. Curry, K. J., Abril, M., Avant, J. B., and Smith, B. J. 2002. Strawberry anthracnose: histopathology of Colletotrichum acutatum and C. fragariae. Phytopathology 92: 1055-1063. Dale, A., and Sjulin, T. M. 1990. Few cytoplasms contribute to North American strawberry cultivars. HortScience 25: 1341-1342. Damm, U., Cannon, P., Woudenberg, J., and Crous, P. 2012a. The Colletotrichum acutatum species complex. Studies in Mycology 73: 37-113. Damm, U., Cannon, P. F., Woudenberg, J., Johnston, P., Weir, B., Tan, Y., Shivas, R., and Crous, P. 2012b. The Colletotrichum boninense species complex. Studies in Mycology 73: 1-36. De Silva, D. D., Crous, P. W., Ades, P. K., Hyde, K. D., and Taylor, P. W. 2017. Life styles of Colletotrichum species and implications for plant biosecurity. Fungal Biology Reviews 31: 155-168. Denoyes-Rothan, B. 1996. Inheritance of resistance to Colletotrichum acutatum in strawberry (Fragaria x ananassa). Pages 809-814 in: III International Strawberry Symposium. Denoyes-Rothan, B., Lafargue, M., Guerin, G., and Clerjeau, M. 1999. Fruit resistance to Colletotrichum acutatum in strawberries. Plant Disease 83: 549-553. Denoyes-Rothan, B., Guérin, G., Lerceteau-Köhler, E., and Risser, G. 2005. Inheritance of resistance to Colletotrichum acutatum in Fragaria × ananassa. Phytopathology 95: 405-412. Dixon, R. A. 2001. Natural products and plant disease resistance. Nature 411:843-847. Encinas-Villarejo, S., Maldonado, A. M., Amil-Ruiz, F., de los Santos, B., Romero, F., Pliego-Alfaro, F., Muñoz-Blanco, J., and Caballero, J. L. 2009. Evidence for a positive regulatory role of strawberry (Fragaria × ananassa) Fa WRKY1 and Arabidopsis At WRKY75 proteins in resistance. Journal of Experimental Botany 60: 3043-3065. Filippone, M., Diaz Ricci, J., Mamanı́ de Marchese, A., Farı́as, R., and Castagnaro, A. 1999. Isolation and purification of a 316 Da preformed compound from strawberry (Fragaria ananassa) leaves active against plant pathogens. FEBS Letters 459: 115-118. Freeman, S., and Katan, T. 1997. Identification of Colletotrichum species responsible for anthracnose and root necrosis of strawberry in Israel. Phytopathology 87: 516-521. Garrido, C., Carbú, M., Fernández‐Acero, F., Boonham, N., Colyer, A., Cantoral, J., and Budge, G. 2009. Development of protocols for detection of Colletotrichum acutatum and monitoring of strawberry anthracnose using real‐time PCR. Plant Pathology 58: 43-51. Giménez, G., and Ballington, J. R. 2002. Inheritance of resistance to Colletotrichum acutatum Simmonds on runners of garden strawberry and its backcrosses. HortScience 37: 686-690. Gooding, H. 1976. Resistance to mechanical injury and assessment of shelf life in fruits of strawberry (Fragaria x ananassa). Horticultural Research 16: 71-82. Grellet-Bournonville, C. F., Martinez-Zamora, M. G., Castagnaro, A. P., and Díaz-Ricci, J. C. 2012. Temporal accumulation of salicylic acid activates the defense response against Colletotrichum in strawberry. Plant Physiology and Biochemistry 54: 10-16. Guidarelli, M., Carbone, F., Mourgues, F., Perrotta, G., Rosati, C., Bertolini, P., and Baraldi, E. 2011. Colletotrichum acutatum interactions with unripe and ripe strawberry fruits and differential responses at histological and transcriptional levels. Plant Pathology 60: 685-697. Gupton, C. L., and Smith, B. J. 1991. Inheritance of resistance to Colletotrichum species in strawberry. Journal of the American Society for Horticultural Science 116: 724-727. Hancock, J., and Luby, J. 1995. Adaptive zones and ancestry of the most important North American strawberry cultivars. Fruit Varieties Journal 49: 85-90. Hancock, J., Hokanson, S., Finn, C., and Hummer, K. 2000. Introducing a supercore collection of wild octoploid strawberries. Pages 77-79 in: IV International Strawberry Symposium. Hancock, J., Callow, P., Dale, A., Luby, J., Finn, C., Hokanson, S., and Hummer, K. E. 2001. From the Andes to the Rockies: native strawberry collection and utilization. HortScience 36: 221-224. Hirai, N., Sugie, M., Wada, M., Lahlou, E. H., Kamo, T., Yoshida, R., Tsuda, M., and Ohigashi, H. 2000. Triterpene phytoalexins from strawberry fruit. Bioscience, Biotechnology, and Biochemistry 64: 1707-1712. Hokanson, S. C., and Maas, J. L. 2001. Strawberry biotechnology. Plant Breeding Reviews 21: 139-180. Horowitz, S., Freeman, S., and Sharon, A. 2002. Use of green fluorescent protein-transgenic strains to study pathogenic and nonpathogenic lifestyles in Colletotrichum acutatum. Phytopathology 92: 743-749. Howard, C., and Albregts, E. 1983. Black leaf spot phase of strawberry anthracnose caused by Colletotrichum gloeosporioides (= C. fragariae). Plant Disease 67: 1144-1146. Howard, C. M. 1972. A strawberry fruit rot caused by Colletotrichum fragariae. Phytopathology 62: 600-602. Howard, C. M., Maas, J., Chandler, C., and Albregts, E. 1992. Anthracnose of strawberry caused by the Colletrotrichum complex in Florida. Plant Disease 76: 976-981. Jacobs, R. L., Adhikari, T. B., Pattison, J., Yencho, G. C., Fernandez, G. E., and Louws, F. J. 2019. Inheritance of resistance to Colletotrichum gloeosporioides and C. acutatum in strawberry. Phytopathology 109: 428-435. Jayasinghe, C., and Fernando, T. 2001. Toxic activity from liquid culture of Colletotrichum acutatum. Mycopathologia 152: 97-101. Jayawardena, R., Hyde, K., Jeewon, R., Li, X., Liu, M., and Yan, J. 2016. Mycosphere Essay 6: Why is it important to correctly name Colletotrichum species? Mycosphere 2: 1076-1092. Khan, A. A. 2002. Characterization of chitinase activities, and cloning, analysis, and expression of genes encoding pathogenesis-related proteins in strawberry. Louisiana State University and Agricultural and Mechanical College, Louisiana. Khan, A. A., and Shih, D. S. 2004. Molecular cloning, characterization, and expression analysis of two class II chitinase genes from the strawberry plant. Plant Science 166: 753-762. Khan, A. A., Shi, Y., and Shih, D. S. 2003. Cloning and partial characterization of a β-1, 3-glucanase gene from strawberry. DNA Sequence 14: 406-412. King, W., Madden, L., Ellis, M., and Wilson, L. 1997. Effects of temperature on sporulation and latent period of Colletotrichum spp. infecting strawberry fruit. Plant Disease 81: 77-84. Leandro, L., Gleason, M., Nutter Jr, F., Wegulo, S., and Dixon, P. 2001. Germination and sporulation of Colletotrichum acutatum on symptomless strawberry leaves. Phytopathology 91: 659-664. Leandro, L., Gleason, M., Nutter Jr, F., Wegulo, S., and Dixon, P. 2003. Influence of temperature and wetness duration on conidia and appressoria of Colletotrichum acutatum on symptomless strawberry leaves. Phytopathology 93: 513-520. Lerceteau-Köhler, E., Guerin, G., and Denoyes-Rothan, B. 2005. Identification of SCAR markers linked to Rca2 anthracnose resistance gene and their assessment in strawberry germplasm. Theoretical and Applied Genetics 111: 862-870. Lewers, K., Turechek, W., Hokanson, S. C., Maas, J., Hancock, J., Serce, S., and Smith, B. 2007. Evaluation of elite native strawberry germplasm for resistance to anthracnose crown rot disease caused by Colletotrichum species. Journal of the American Society for Horticultural Science 132: 842-849. Maas, J. L., and Galletta, G. J. 1988. Germplasm evaluation for resistance to fungus-incited diseases. Pages 461-472 in: International Strawberry Symposium. MacKenzie, S., Mertely, J., and Peres, N. 2009. Curative and protectant activity of fungicides for control of crown rot of strawberry caused by Colletotrichum gloeosporioides. Plant Disease 93: 815-820. MacKenzie, S., Legard, D., Timmer, L., Chandler, C., and Peres, N. 2006. Resistance of strawberry cultivars to crown rot caused by Colletotrichum gloeosporioides isolates from Florida is nonspecific. Plant Disease 90: 1091-1097. Mangandi, J., Peres, N. A., and Whitaker, V. M. 2015. Identifying resistance to crown rot caused by Colletotrichum gloeosporioides in strawberry. Plant Disease 99: 954-961. Milholland, R. 1982. Histopathology of strawberry infected with Colletotrichum fragariae. Phytopathology 72: 1434-1439. Münch, S., Lingner, U., Floss, D. S., Ludwig, N., Sauer, N., and Deising, H. B. 2008. The hemibiotrophic lifestyle of Colletotrichum species. Journal of Plant Physiology 165: 41-51. Muñoz, C., Hoffmann, T., Escobar, N. M., Ludemann, F., Botella, M. A., Valpuesta, V., and Schwab, W. 2010. The strawberry fruit Fra a allergen functions in flavonoid biosynthesis. Molecular Plant 3: 113-124. Musidlowska-Persson, A., Alm, R., and Emanuelsson, C. 2007. Cloning and sequencing of the Bet v 1-homologous allergen Fra a1 in strawberry (Fragaria x ananassa) shows the presence of an intron and little variability in amino acid sequence. Molecular Immunology 44: 1245-1252. Neal, J., Ko, H., Gomez, A., De Faveri, J., Verbyla, A., Mayer, R., and Herrington, M. 2016. Testing strawberry genotypes for resistance to Colletotrichum gloeosporioides in Queensland. Pages 743-750 in: VIII International Strawberry Symposium. Nicoll, M., and Galletta, G. 1987. Variation in growth and flowering habits of June bearing and everbearing strawberries. Journal of the American Society for Horticultural Science 112: 872-880. O'Connell, R. J., Thon, M. R., Hacquard, S., Amyotte, S. G., Kleemann, J., Torres, M. F., Damm, U., Buiate, E. A., Epstein, L., and Alkan, N. 2012. Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses. Nature Genetics 44: 1060-1065. Peres, N., Timmer, L., Adaskaveg, J., and Correll, J. 2005. Lifestyles of Colletotrichum acutatum. Plant Disease 89: 784-796. Perfect, S. E., Hughes, H. B., O'Connell, R. J., and Green, J. R. 1999. Colletotrichum: a model genus for studies on pathology and fungal–plant interactions. Fungal Genetics and Biology 27: 186-198. Rahman, M., and Louws, F. J. 2017. Epidemiological significance of crown rot in the fruiting field in relation to Colletotrichum gloeosporioides infection of strawberry nursery plants. Plant Disease 101: 907-915. Rahman, M., Ballington, J., and Louws, F. 2013. Role of foliar hemibiotrophic and fruit resistance in anthracnose‐resistant strawberry genotypes for annual hill plasticulture systems. Annals of Applied Biology 163: 102-113. Rojas, E. I., Rehner, S. A., Samuels, G. J., Van Bael, S. A., Herre, E. A., Cannon, P., Chen, R., Pang, J., Wang, R., and Zhang, Y. 2010. Colletotrichum gloeosporioides s.l. associated with Theobroma cacao and other plants in Panama: multilocus phylogenies distinguish host-associated pathogens from asymptomatic endophytes. Mycologia 102: 1318-1338. Salazar, S. M., Grellet, C., Chalfoun, N. R., Castagnaro, A. P., and Ricci, J. D. 2013. Avirulent strain of Colletotrichum induces a systemic resistance in strawberry. European Journal of Plant Pathology 135: 877-888. Salazar, S. M., Castagnaro, A. P., Arias, M., Chalfoun, N., Tonello, U., and Ricci, J. D. 2007. Induction of a defense response in strawberry mediated by an avirulent strain of Colletotrichum. European Journal of Plant Pathology 117: 109-122. Seijo, T. E., Chandler, C. K., Mertely, J. C., Moyer, C., and Peres, N. A. 2008. Resistance of strawberry cultivars and advanced selections to anthracnose and Botrytis fruit rots. Proceedings of the Florida State Horticultural Society 121: 246-248. Shi, Y., Zhang, Y., and Shih, D. S. 2006. Cloning and expression analysis of two β-1, 3-glucanase genes from strawberry. Journal of Plant Physiology 163: 956-967. Shu, L.-J., Liao, J.-Y., Lin, N.-C., and Chung, C.-L. 2018. Identification of a strawberry NPR-like gene involved in negative regulation of the salicylic acid-mediated defense pathway. PLoS One 13.10 Simpson, D., Winterbottom, C., Bell, J., and Maltoni, M. 1994. Resistance to a single UK isolate of Colletotrichum acutatum in strawberry germplasm from Northern Europe. Euphytica 77: 161-164. Sjulin, T., and Dale, A. 1987. Genetic diversity of North American strawberry cultivars. Journal of the American Society for Horticultural Science 112: 375-385. Smith, B., and Black, L. 1987. Resistance of strawberry plants to Colletotrichum fragariae affected by environmental conditions. Plant Disease 71: 834-837. Smith, B., Black, L., and Galletta, G. 1990. Resistance to Colletotrichum fragariae in strawberry affected by seedling age and inoculation method. Plant Disease 74: 1016-1021. Smith, B., Gupton, C., Galletta, G., Maas, J., Enns, J., Ballington, J., Constantin, R., DiVittorio, T., and Himelrick, D. 1998. 'Pelican' strawberry. HortScience 33: 1082-1084. Smith, B. J. 2008. Epidemiology and pathology of strawberry anthracnose: a North American perspective. HortScience 43: 69-73. Sreenivasaprasad, S., Sharada, K., Brown, A., and Mills, P. 1996. PCR‐based detection of Colletotrichum acutatum on strawberry. Plant Pathology 45: 650-655. Sutton, B. 1992. The genus Glomerella and its anamorph Colletotrichum. Pages 1-26 in: Colletotrichum Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International, Wallingford, UK. Turechek, W. W., Peres, N. A., and Werner, N. A. 2006. Pre- and post-infection activity of pyraclostrobin for control of anthracnose fruit rot of strawberry caused by Colletotrichum acutatum. Plant Disease 90: 862-868. Ureña-Padilla, A., MacKenzie, S., Bowen, B., and Legard, D. 2002. Etiology and population genetics of Colletotrichum spp. causing crown and fruit rot of strawberry. Phytopathology 92: 1245-1252. Vincent, A., Dayan, F., Maas, J., and Wedge, D. 1999. Detection and isolation of antifungal compounds in strawberry inhibitory to Colletotrichum fragariae. Advances in Strawberry Research 18: 47-55. Von Arx, J. A. 1970. A revision of the fungi classified as Gloeosporium. Bibliotheca Mycologica 24: 1-203. Weir, B., Johnston, P., and Damm, U. 2012. The Colletotrichum gloeosporioides species complex. Studies in Mycology 73: 115-180. Wheeler, H., and Luke, H. 1955. Mass screening for disease-resistant mutants in oats. Science 122: 1229-1229. Wikee, S., Cai, L., Pairin, N., McKenzie, E. H., Su, Y.-Y., Chukeatirote, E., Thi, H. N., Bahkali, A. H., Moslem, M. A., and Abdelsalam, K. 2011. Colletotrichum species from Jasmine (Jasminum sambac). Fungal Diversity 46: 171-182. Yoshida, S., Hiradate, S., Fujii, Y., and Shirata, A. 2000. Colletotrichum dematium produces phytotoxins in anthracnose lesions of mulberry leaves. Phytopathology 90: 285-291. Zhang, G.-M., Fang, B.-H., Chen, H., and Li, X.-I. 2012. Characteristics of the toxin extracted from liquid culture of Colletotrichum capsici f. nicotianae. Applied Biochemistry and Biotechnology 167: 52-61. Zhang, Q.-Y., Zhang, L.-Q., Song, L.-L., Duan, K., Li, N., Wang, Y.-X., and Gao, Q.-H. 2016. The different interactions of Colletotrichum gloeosporioides with two strawberry varieties and the involvement of salicylic acid. Horticulture Research 3: 1-10. Zhang, X., Batzer, J. C., Li, X., Peres, N. A., and Gleason, M. L. 2019. Validation of a Florida strawberry anthracnose fruit rot (AFR) warning system in Iowa. Plant Disease 103: 28-33. Zhang, Y., and Shih, D. S. 2007. Isolation of an osmotin-like protein gene from strawberry and analysis of the response of this gene to abiotic stresses. Journal of Plant Physiology 164: 68-77. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63628 | - |
dc.description.abstract | 草莓炭疽病的病原為 Colletotrichum spp.,可感染草莓全株,在各個生長期都造成危害。臺灣田間炭疽病菌族群以 C. siamense 為主 (佔75%),C. fructicola 次之 (佔11%)。臺灣的主流栽培品種 ‘桃園 1 號’ 對炭疽病十分感病,因此產業上對於具有良好園藝性狀又抗病的新品種有殷切需求。國內草莓種原保存於桃園區農業改良場新埔工作站及苗栗區農業改良場,可做為抗病育種的親本來源,但目前對其抗感病性並不明瞭。為篩選具抗病力之育種潛力親本,本研究建立可同時評估葉部炭疽病及冠腐病的接種系統,以本土代表性菌株 C. siamense ML133 及 C. fructicola ML356 接種種原庫中的 27 個品種 (系),發現 ‘福羽’、‘TYS16091’ 及 ‘TYS16109’ 抗性較佳。為加速抗病育種的進程,以炭疽病菌培養濾液處理草莓葉圓片之方式,建立抗性評估快篩法,結果顯示以第二展開葉為材料表現最為穩定。比較 9 個草莓品種對兩代表性菌株之接種試驗與葉圓片試驗結果,發現兩者並無顯著相關性,因此利用葉圓片來進行快篩技術的實際應用,還需更多改良與重複測試。目前有關草莓與炭疽病菌交互作用的研究,幾乎僅限於 C. acutatum 及 C. gloeosporioides,為瞭解本土代表性草莓炭疽病菌 C. siamense 與 C. fructicola 侵染抗病、感病草莓寄主的顯微構造及引發之抗性反應,應用台盼藍 (trypan blue) 及二氨基聯苯胺 (diaminobenzidine, DAB) 染色進行早期侵染構造觀察,發現抗性品種 ‘福羽’、‘Tufts’ 與 ‘Solana’ 葉片上的孢子發芽率及壓器產生率均顯著低於感病品種 ‘桃園 1 號’,而四個受試品種被感染之葉部組織均未觀察到過氧化氫的累積,顯示 ‘福羽’、‘Tufts’ 與 ‘Solana’ 所帶抗性可以有效延緩 C. siamense 與 C. fructicola的侵染,但其機制可能並非來自過敏性反應。 | zh_TW |
dc.description.abstract | Strawberry (Fragaria × ananassa Duch.) anthracnose is caused by Colletotrichum spp. The pathogen can infect the whole strawberry plant at all growth stages. The predominant Colletotrichum spp. causing strawberry anthracnose in Taiwan are C. siamense and C. fructicola that account for 75% and 11% of the anthracnose cases, respectively. The most popular cultivar in Taiwan, ‘Taoyuan no.1’, is highly susceptible to anthracnose. The newly selected cultivars possessing both desirable horticultural traits and disease resistance are necessary in the industry. The stock of strawberry germplasms in Taiwan were kept in Taoyuan and Miaoli District Agricultural and Research Extension Stations, which are potential donor candidates for resistance-breeding. However, their susceptibilities to anthracnose are unclear. To screen the potential resistance lines for breeding, we established an inoculation system which can evaluate their susceptibilities according to the symptoms on the foliage and crown at the same time. Among the 27 cultivars inoculated with C. siamense ML133 and C. fructicola ML356, two representative local isolates, ‘Fukuba’, ‘TYS16091’ and ‘TYS16109’ possess relatively higher resistance. To facilitate the process of breeding, a leaf disc assay method (strawberry leaf discs treated with the culture filtrate of Colletotrichum spp.) was developed. Stable results were achieved with the use of the second youngest leaves. There was no significant correlation between the results from Colletotrichum spp. inoculation and the leaf disc assay of 9 cultivars, suggesting that the leaf disc assay method needs further modification. Previous research on host-pathogen interactions between strawberry and Colletotrichum spp. mostly focused on C. acutatum and C. gloeosporioides. In order to understand the microscopic structures and the induced defense responses during infection of the local species C. siamense and C. fructicola in strawberry, trypan blue and 3',3'-diaminobenzendine (DAB) staining were used to observe the early-stage infection structures of the pathogen. Significantly lower spore germination rates and appressorium formation rates were observed from the resistant cultivars ‘Fukuba’, ‘Tufts’, and ‘Solana’ than the susceptible cultivar ‘Taoyuan no.1’, and no hydrogen peroxide was accumulated in the infected leaf tissues of the four tested cultivars. The resistance of ‘Fukuba’, ‘Tufts’, and ‘Solana’, which can effectively delay the infection of C. siamense and C. fructicola, is unlikely associated with the hypersensitive reactions. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T17:15:16Z (GMT). No. of bitstreams: 1 ntu-109-R04633015-1.pdf: 598420044 bytes, checksum: 5fefc4cfc8c5c250a13ad5fe3a13f0b7 (MD5) Previous issue date: 2020 | en |
dc.description.tableofcontents | CONTENTS
論文口試委員審定書 I 致謝 II 中文摘要 III ABSTRACT IV CONTENTS VI LIST OF TABLES IX LIST OF FIGURES X Chapter 1 前言 1 Chapter 2 前人研究 3 2.1 臺灣的草莓品種 3 2.2 主要草莓病蟲害 4 2.3 草莓炭疽病報導與病原菌鑑定 6 2.4 草莓品種對炭疽病之抗性測試 8 2.5 草莓抗炭疽病機制 9 2.5.1 物理構造上的防禦 9 2.5.2 草莓代謝物抗炭疽病菌物質 10 2.5.3 草莓基因與蛋白質層次的抗性 11 2.6 炭疽病菌毒性代謝物之運用 12 2.6.1 毒性代謝物運用於抗性篩檢 12 2.6.2 毒性代謝物運用於誘發草莓抗性 13 Chapter 3 材料方法 14 3.1 試驗植株 14 3.2 試驗菌株與傳統全株接種 14 3.3 製備炭疽病菌無菌培養濾液 15 3.4 葉圓片測定法 15 3.4.1 不同生育期葉片之影響 16 3.4.2 評估葉圓片測定法用於快篩檢驗 16 3.5 炭疽病菌於草莓葉片之侵染觀察 17 Chapter 4 結果 18 4.1 傳統全株接種試驗 18 4.2 葉圓片測定法 19 4.2.1 不同生育期葉片之影響 19 4.2.2 葉圓片測定法與傳統接種結果比較 19 4.3 炭疽病菌於草莓葉片之侵染觀察 20 4.3.1 過氧化氫累積觀察 20 4.3.2 孢子發芽率與壓器產生率 20 Chapter 5 討論 22 5.1 種原庫品種抗性篩檢 22 5.2 冠部抗性檢測之必要性 25 5.3 快篩檢定法 26 5.4 過氧化物累積觀察 27 5.5 分生孢子發芽率與壓器產生率 28 5.6 炭疽病菌之侵染構造 29 5.7 結論 30 References 31 Tables 48 Figures 52 LIST OF TABLES Table 1. Cultivars and lines in the screening assay………..……………………………..49 Table 2. Spore germination rate of Colletotrichum siamense ML133, C. fructicola ML356 and C. karstii ML351 on four strawberry cultivars on 1 and 3 days post inoculation (dpi)………………………………………………………………………..….……….50 Table 3. Appressorium formation rate of Colletotrichum siamense ML133, C. fructicola ML356 and C. karstii ML351 on four strawberry cultivars on 1 and 3 days post inoculation (dpi) ………………………………………………………………………51 LIST OF FIGURES Figure 1. Disease severity index.………………………………………………………53 Figure 2. Leaf and crown symptoms caused by inoculation with C. siamense ML133. ……………………………………………………………………….………..54 Figure 3. Leaf and crown symptoms caused by inoculation with C. fructicola ML356.…………………………………………………………………………………55 Figure 4. Simultaneous inoculation of C. siamense ML133 on ‘Taoyuan no.1’.….…..56 Figure 5. Disease severity index of 27 cultivars/lines inoculated with C. siamense ML133 and C. fructicola ML356………………………………….…………………...57 Figure 6. Leaves at different growth stages treated with the culture filtrates of C. siamense ML133 and C. fructicola ML356.…………………………………………...59 Figure 7. Leaf disc assay using strawberry leaves at different growth stages..………. 60 Figure 8. Correlation between the results from spore suspension inoculation and the leaf disc assay.………………........………………………………………………………....61 Figure 9. Detection of the accumulation of hydrogen peroxide at C. siamense ML133 spore germination sites by trypan blue and 3, 3 -diaminobenzidine (DAB) staining.....62 Figure 10. Detection of the accumulation of hydrogen peroxide in strawberry leaves inoculated with Colletotrichum spp. by 3, 3-diaminobenzidine (DAB) staining…..….63 Figure 11. Detection of the accumulation of hydrogen peroxide at spore germination sites by trypan blue and 3, 3 -diaminobenzidine (DAB) staining.……………………..65 Figure 12. Leaf symptoms caused by Colletotrichum spp. on strawberry cultivars ‘Taoyuan no.1’, ‘Fukuba’, ‘Tufts’, and ‘Solana’.……………………..……………….66 Figure 13. Spore germination rate and appressorium formation rate of C. siamense ML133 on ‘Taoyuan no.1’ and ‘Fukuba’………………………………………………67 | |
dc.language.iso | zh-TW | |
dc.title | 臺灣草莓種原庫對炭疽病菌之抗性篩檢 | zh_TW |
dc.title | Screening of strawberry germplasm in Taiwan for resistance to Colletotrichum spp. | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳昭瑩,洪挺軒,林乃君,吳岱融 | |
dc.subject.keyword | 炭疽病菌,種原庫,抗病性篩檢,葉圓片測定法,侵染構造,過敏性反應, | zh_TW |
dc.subject.keyword | Colletotrichum spp.,germplasm,resistance screening,leaf disc assay,infection structure,hypersensitive reaction, | en |
dc.relation.page | 67 | |
dc.identifier.doi | 10.6342/NTU202000720 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2020-04-08 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
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