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Effects of Raw264.7 macrophages pretreated with Momordica charantia L. extracts on inflammatory mediators secretion and lipid metabolism of 3T3-L1 adipocytes
|Publication Year :||2012|
Obesity is often accompanied by several disorders, such as diabetes, hyperlipidemia, and hypertension. There is considerable evidence that obesity is a state of chronic low grade inflammation. Obese adipose tissue exhibits the inflammatory changes characterized by adipocyte hypertrophy and increased infiltration of macrophages. Bitter gourd (Momordica Charantia) is a tropical vegetable that has also been used in a traditional medicine for treating diabetes. In this study, the ethyl acetate (EA) and water extracts (WE) of bitter gourd powder (BGP) were used to determine the anti-obesity and anti-inflammatory effects on 3T3-L1 adipocytes, Raw264.7 macrophages and their interaction.
The pro-inflammatory cytokine secretions were examined after 24 hours treatment of BGP extracts. We showed that BGP-EA decreased the production of MCP-1 and IL-6 in 3T3-L1 adipocytes. TNF-α and IL-6 secretions in LPS-stimulated Raw264.7 was also inhibited. Inversely, BGP-WE elevated the production of pro-inflammatory cytokines both in 3T3-L1 and LPS-stimulated Raw264.7. On the other hand, Both BGP extracts stimulate basal lipolysis of 3T3-L1 after 24 hours treatment. The BGP-EA promoted the mRNA expression of β-oxidation factors. Unlike BGP-EA, BGP-WE suppressed the expression of lipogenic and β-oxidation factors in 3T3-L1. This study provides evidence for the anti-inflammatory effect of BGP-EA but pro-inflammatory effect of BGP-WE on macrophages and adipocytes. Increased lipolysis also suggested the anti-obesity effect of BGP extracts.
To further investigate the interaction between adipocytes and macrophages, Raw 264.7 cells were pretreated with different concentrations of BGP-EA and BGP-WE then cocultured with 3T3-L1. Coculture of 3T3-L1 adipocytes and RAW 264.7 macrophages markedly enhanced lipolysis of adipocytes and the production of TNF-α, MCP-1 and IL-6 compared with control cultures. However, the secretion of these cytokines was decreased and lipolysis was inhibited when Raw264.7 cells were pretreated with BGP extracts. The mRNA expression of pro-inflammatory factors COX-2 was also diminished in 3T3-L1 adipocytes which were cocultured with BGP extracts-pretreated Raw264.7 cells. The inflammatory changes and the disorder of lipid metabolism induced by the interaction between adipocytes and macrophages were improved by the pretreatment of Raw264.7 with BGP extracts. The BGP may have potential to ameliorate the inflammation in obese adipose tissue.
|Appears in Collections:||生化科技學系|
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