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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李佳音(Chia Yin Lee) | |
| dc.contributor.author | Ming-Hsu Liu | en |
| dc.contributor.author | 劉明煦 | zh_TW |
| dc.date.accessioned | 2021-06-16T17:13:49Z | - |
| dc.date.available | 2017-08-01 | |
| dc.date.copyright | 2012-08-27 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-20 | |
| dc.identifier.citation | 王韻婷 (2000). 高分子生物塑膠生產菌之分離，特性分析及高分子合成基
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63546 | - |
| dc.description.abstract | 本研究利用六株格蘭氏陰性細菌Duganella zoogloeoides TF1-1、Pseudomonas mosselii TO7、Pseudomonas nitroreducens PO6、Pseudomonas citronellolis PO21、Duganella zoogloeoides TS18及Paracoccus versutus GG45培養在含生質柴油副產物的礦物鹽培養基中48小時，觀察聚羥基烷酸酯的累積情形。其中以TF1-1及TO7兩株菌的PHA含量最高，分別為55 mol %及48 mol %。利用氣相層析分析聚合物單體組成，TF1-1所生成的PHA具有3-HB單體(100 mol%)，TO7生合成的PHA單體組成以3-HO (83 mol %)及3-HB(15 mol %)為主。以1H 及13C NMR解析聚合物結構，證實TF1-1所生合成的聚合物為PHB，TO7生合成的PHA，具有P(3HB)及P(3HO)的組成，與氣相層析分析結果一致。另外以傅立葉轉換紅外線光譜儀鑑定此兩株菌生產的PHA之官能基，證實具有PHA的C=O官能基訊號、C-O-C伸張模式的訊號，CH、CH2及CH3皆有吸收鋒出現。凝膠滲透層析儀測定TF1-1及TO7的PHA之廣分佈係數分別是1.30及1.38。熱性質分析結果顯示TO7及TF1-1的PHA產物裂解溫度分別為281.52oC及222.43oC；微差熱掃描分析結果顯示TF1-1的PHA產物比一般短鏈PHA的結晶度為低，具有較廣之應用性。為研究培養溫度及培養時間對於菌體內PHA分子量變化的影響，本研究以TO7為研究對象，以辛酸鈉為單一碳源觀察，結果顯示廣分佈係數無顯著的變化趨勢，但分子量分佈情形在高溫下培養有變高的趨勢，證實培養溫度對於分子量變化有影響。本研究結果顯示TF1-1及TO7兩株菌可以利用生質柴油副產物為碳源生成聚羥基烷酸酯，有較低的廣分佈係數，具備工業發展的潛力。 | zh_TW |
| dc.description.abstract | The six gram negative bacteria strains, Duganella zoogloeoides TF1-1, Pseudomonas mosselii TO7, Pseudomonas nitroreducens PO6, Pseudomonas citronellolisPO21, Duganella zoogloeoides TS18 and Paracoccus versutus GG45 were cultured in the MS medium containing biodiesel by-product to accumulate PHA. Among them, strains of TF1-1 and TO7 have the higher PHA content which were 55 mol % and 48 mol % , respectively. Gas chromatography revealed the monomer composition of polymer from TF1-1 utilizing BDF by-product was completely 3-HB (100 mol%), whereas the monomer unit of TO7 is primarily 3-HO (83 mol %) and 3-HB (15 mol %). NMR displayed the pattern of polymer produced by TF1-1 identical to the spectra of PHB. NMR spectrum of polymer produced by TO7 corroborates the composition and the expected structure analyzed by GC analysis. FTIR spectra show the absorption band CH , CH2 , CH3, ester bond and C-O-C band stretching. GPC analysis showed PDI values of the polymer produced by TF1-1 and TO7 were 1.3 and 1.38. The culture temperature and time have no predominant effects on the PDI value change. But molecular weight distribution curve gradually changed to a bimodal after 44 h at 35℃, suggesting the cultivation temperature is a factor involved in molecular weight change. This study showed the TF1-1 and TO7 will be potential candidates for industrial production of PHA with low PDI value and lower crystallinity from inexpensive waste substrate. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T17:13:49Z (GMT). No. of bitstreams: 1 ntu-101-R97623023-1.pdf: 1570775 bytes, checksum: 104994fc518cc0866c720a25ab44480d (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 口試委員審定書 i
中文摘要 iii Abstract iv 目錄…….. v 表次…… viii 圖次…… ix 附錄圖次 x 縮寫表 xi 壹、 前言 1 一、PHA物理性質和化學性質 1 1. PHA的結構 1 2. PHA的生物可分解性及生物可相容性 1 3. PHA的種類 2 4. PHA的分子量 3 5. PHA合成酶 3 6. 短鏈 PHA生合成途徑 3 7. 中長鏈PHA生合成途徑 4 二、PHA的應用 4 三、生質概念的興起 5 1. 生質材料的發展現況 5 2. 利用廉價的碳源生產PHA 6 四、生質柴油的介紹 7 1. 生質柴油的發展現況 7 2. 生質柴油原料 8 3. 生質柴油的生產技術 9 4. 生質柴油的未來展望 9 五、生質柴油副產物的加值利用 10 1. 生質柴油副產物背景介紹 10 2. 生質柴油副產物加值為動物飼料 10 3. 生質柴油副產物加值成為1,3-propanediol 10 4. 生質柴油副產物加值成為citric acid 11 5. 生質柴油副產物加值成為氫氣及小分子燃料 11 6. 生質柴油副產物加值成為聚羥基烷酸酯 12 7. 生質柴油副產物加值成為Docosahexaenoic acid 12 六、研究目的 12 貳、 實驗材料與方法 14 一、實驗材料 14 1. 實驗菌株 14 2. 培養基 14 3. 碳源 15 4. 藥品與試劑 15 5. 儀器 16 二、實驗方法 17 1. 初步篩選PHA累積情形 17 2. 螢光光譜儀鑑定PHA累積 18 3. PHA萃取 (PHA extraction) 18 4. 菌體乾重 (Biomass)、PHA含量 (PHA content)、PHA產率 (PHA productivity)測定 19 5. 超導核磁共振 (Nuclear Magnetic Resonance, NMR) 19 6. 控溫式傅立葉轉換紅外線光譜儀 (Fourier transform infrared resonance, FTIR) 19 7. 氣相層析儀 (Gas Chromatography, GC) 19 8. 熱重分析儀 (Thermogravimetric analysis, TGA) 21 9. 微差熱掃描分析儀 (Differential Scanning calorimetry, DSC) 21 10. 凝膠滲透層析儀 (Gel Permeation Chromatography, GPC) 21 11. 廣分佈係數(Polydispersity index, PDI)調控實驗 22 參、實驗結果 23 一、生質柴油副產物及大豆油利用率佳之PHA累積菌株 23 二、甘油及脂肪酸鈉利用率佳之PHA累積菌株 23 三、利用氣相層析儀分析聚合物的單體組成 24 四、生質柴油副產物為單一碳源的最適化培養條件探討 25 五、核磁共振及傅立葉轉換紅外線光譜儀解析聚合物結構 26 六、以生質柴油副產物當作碳源生成PHA之熱性質及分子量分析 27 七、 Pseudomonas mosselii TO7以辛酸鈉為單一碳源下不同溫度及時間培養所產生之聚羥基烷酸酯的廣分佈係數的影響 27 肆、討論 29 一、TF1-1以生質柴油副產物為碳源之菌體生長及PHA代謝途徑 29 二、TO7以生質柴油副產物為碳源之菌體生長及PHA代謝途徑 30 三、培養溫度及時間對於PHA分子量的影響 31 伍、結論 33 陸、參考文獻 34 柒、表…….. 42 捌、圖………… 56 玖、附錄圖 73 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用生質柴油副產物生產聚羥基烷酸酯及其物性分析 | zh_TW |
| dc.title | Production and characterization of polyhydroxyalkanoates utilizing biodiesel by-product | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林榮流,劉?德 | |
| dc.subject.keyword | 聚羥基烷酸酯, | zh_TW |
| dc.subject.keyword | polyhydroxyalkanoates, | en |
| dc.relation.page | 77 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-08-20 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農業化學研究所 | zh_TW |
| 顯示於系所單位： | 農業化學系 | |
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