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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳凱儀(Kai-Yi Chen) | |
| dc.contributor.author | Po-Ying Chu | en |
| dc.contributor.author | 朱柏穎 | zh_TW |
| dc.date.accessioned | 2021-06-16T16:41:54Z | - |
| dc.date.available | 2012-09-03 | |
| dc.date.copyright | 2012-09-03 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-28 | |
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JPN J Breed 22:209-216. Joehanes, R., J.C. Nelson. (2008) QGene 4.0, an extensible Java QTL-analysis platform. Bioinformatics 24:2788-2789. Kao, C.H., Z.B. Zeng. (2002) Modeling epistasis of quantitative trait loci using Cockerham's model. Genetics 160:1243-1261. Koornneef, M., L. Bentsink, H. Hilhorst. (2002) Seed dormancy and germination. Curr Opin in Plant Biol 5:33-36. Lander, E.S., D. Botstein. (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185-99. Li, B., M.E. Foley. (1997) Genetic and molecular control of seed dormancy. Trends Plant Sci 2:384-389. Li, C., A. Zhou, T. Sang. (2006) Genetic analysis of rice domestication syndrome with the wild annual species, Oryza nivara. New Phytol 170:185-93. Lin, S.Y., T. Sasaki, M. Yano. (1998) Mapping quantitative trait loci controlling seed dormancy an heading date in rice, Oryza sativa L., using backcrosses inbred lines. Theor Appl Genet 96:997-1003. Lincoln, S.E., M.J. Daly, E.S. Lander. (1993) Mapping genes controlling quantitative traits using Mapmaker/QTL version 1.1: a tutorial and reference manual. Whitehead Institute for Biomedical Research Technical Report. Lippman, Z., S.D. Tanksley. (2001) Dissecting the genetic pathway to extreme fruit size in tomato using a cross between the small-fruited wild species Lycopersicon pimpinellifolium and L. esculentum var. Giant Heirloom. Genetics 158:413-422. Martinez-Andujar, C., M.I. Ordiz, Z. Huang, M. Nonogaki, R.N. Beachy, H. Nonogaki. (2011) Induction of 9-cis-epoxycarotenoid dioxygenase in Arabidopsis thaliana seeds enhances seed dormancy. Proc Natl Acad Sci USA. Marzougui, S., K. Sugimoto, U. Yamanouchi, M. Shimono, T. Hoshino, K. Hori, M. Kobayashi, K. Ishiyama, M. Yano. (2012) Mapping and characterization of seed dormancy QTLs using chromosome segment substitution lines in rice. Theor Appl Genet 124:893-902. McCarty, D.R., C.B. Carson, P.S. Stinard, D.S. Robertson. 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Cambridge University Press. Sugimoto, K., Y. Takeuchi, K. Ebana, A. Miyao, H. Hirochika, N. Hara, K. Ishiyama, M. Kobayashi, Y. Ban, T. Hattori, M. Yano. (2010) Molecular cloning of Sdr4, a regulator involved in seed dormancy and domestication of rice. Proc Natl Acad Sci USA 107:5792-7. Taiz, L., E. Zeiger. (2010) Topic 23.18: Types of seed dormancy and the roles of environmental factors. Plant Physiol, Fifth Edition Online. http://www.plantphys.net. Accessed [2012/7/19]. Thomson, M.J., T.H. Tai, A.M. McClung, X.H. Lai, M.E. Hinga, K.B. Lobos, Y. Xu, C.P. Martinez, S.R. McCouch. (2003) Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet 107:479-93. Tinker, N.A., D.E. Mather. (1995) MQTL: software for simplified composite interval mapping of QTL in multiple environments. Journal of Agricultural Genomics 1:1-5. Wan, J., T. Nakazaki, K. 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Heredity (Edinb) 84:525-537. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63440 | - |
| dc.description.abstract | 稻穀在收穫前若遭遇高溫溼熱的氣候，容易發芽，此現象稱為穗上發芽，穗上發芽的程度與個別「基因型/品系」的種子休眠性相關。本論文的目的是在高雄及花蓮兩個育種族群中鑑定出已知的水稻種子休眠與穗上發芽數量性狀基因座。高雄族群由高雄143號與高雄146號的雜交後代組成，而花蓮族群則由臺稉16號與臺農71號的雜交後代組成；高雄143號與臺稉16號在各自的雜交組合中皆為貢獻強種子休眠性的親本。為了進行已知數量性狀基因座的遺傳定位，分子標記位置的選擇是基於十篇文獻所記載的主效數量性狀基因座的位置，而高雄族群與花蓮族群各有十八個與十三個SNP分子標記成功地完成基因型鑑定。本論文並應用selective genotyping以節省基因型鑑定的成本。
結果顯示在花蓮族群偵測到兩個種子休眠數量性狀基因座，分別與位於第一和第四條染色體上的分子標記snp01和snp07連鎖。與snp01分子標記連鎖的數量性狀基因座可解釋較大的外表型變異，其增進種子休眠性的對偶基因來自臺稉16號；此一數量性狀基因座亦與兩個已知的水稻種子休眠數量性狀基因座sd1和Sdr6、以及一個阿拉伯芥種子休眠基因DOG1在水稻的同源基因OsDOG1L-2位於相同的染色體區間。然而，沒有任何已知的數量性狀基因座能在高雄族群中被偵測到。 | zh_TW |
| dc.description.abstract | Rice grains can germinate before harvest in hot and humid weather; this phenomenom is called pre-harvest sprouting. The degree of pre-harvest sprouting relates to seed dormancy of a genotype/line. The object of this thesis was to identify known rice QTLs for seed dormancy and pre-harvest sprouting in two breeding populations － Kaohsiung and Hualian population. Kaohsiung population was made from a cross between Kaohsiung 143 and Kaohsiung 146, and Hualian population was made from a cross between Taiken 16 and Tainung 71. Kaohsiung 143 and Taiken 16 were the donor parents contributing stronger seed dormancy in each cross. For mapping known QTLs, the locations of molecular markers were assigned according to the chromosomal regions of known major QTLs in ten literatures; eighteen and thirteen SNP molecular markers were successfully genotyped in Kaohsiung and Hualien populations, respectively. In addition, selective genotyping was applied to save the cost for marker genotyping.
The result showed that two seed dormancy QTLs were identified in Hualien population; they were linked to the snp01 marker and the snp07 marker on chromosome 1 and 4, respectively. The QTL linked to the snp01 marker explained larger phenotypic variance, and its allele from Taiken 16 was able to enhance seed dormancy. This QTL also co-localized with two known rice QTLs for seed dormancy (sd1 and Sdr6) and one rice gene homologous to the Arabidopsis seed dormancy gene DOG1 (OsDOG1L-2). However, no known QTL was identified in Kaohsiung population. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T16:41:54Z (GMT). No. of bitstreams: 1 ntu-101-R99621121-1.pdf: 2353496 bytes, checksum: 87d4e77afde09292deacd6dde576da4e (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 摘要 v Abstract vi 目錄 vii 第一章 緒論 1 第一節 水稻穗上發芽研究在臺灣的現況 1 第二節 種子休眠性的生理研究 1 第三節 水稻種子休眠性與水稻穗上發芽的遺傳研究 4 第一篇、Dong et al. (2003) 5 第二篇、Gao et al. (2008) 6 第三篇、Cai and Morishima (2000) 7 第四篇、Gu et al. (2004) 8 第五篇、Guo et al. (2004) 9 第六篇、Li et al. (2006) 10 第七篇、Lin et al. (1998) 10 第八篇、Thomson et al. (2003) 12 第九篇、Wan et al. (2005) 13 第十篇、Wan et al. (2006) 14 第十一篇、Sugimoto et al. (2010) 15 綜合討論 16 第四節 Selective Genotyping概論 16 第五節 Selective Genotyping前人提出的分析方式及統計模式 17 第二章 研究目的 19 第三章 材料與方法 20 第一節 目標性狀 20 第二節 定位族群親本介紹 20 高雄改良場族群 20 花蓮改良場族群 21 第三節 定位族群 22 高雄改良場族群 22 花蓮改良場族群 22 第四節 排除花蓮改良場族群中疑似混到臺農71號的品系 22 第五節 發芽試驗 24 高雄改良場族群 24 花蓮改良場族群 25 第六節 發芽率鑑定 25 第七節 Selective Genotyping所選的品系 26 第八節 分子標記的設計 26 高雄改良場族群 27 花蓮改良場族群 28 第九節 基因型鑑定平臺 29 第十節 分子標記的卡方適合度檢定及卡方獨立性檢定 30 卡方適合度檢定 30 卡方獨立性檢定 31 第十一節 水稻種子休眠數量性狀基因座定位 32 第四章 結果 34 第一節 發芽率鑑定 34 第二節 基因型鑑定 34 第三節 分子標記的卡方適合度檢定及卡方獨立性檢定 36 卡方適合度檢定 36 卡方獨立性檢定 36 第四節 水稻種子休眠數量性狀基因座定位 36 第五章 討論 38 第一節 發芽率鑑定 38 第二節 基因型鑑定 38 第三節 分子標記的卡方適合度檢定及卡方獨立性檢定 40 卡方適合度檢定 40 卡方獨立性檢定 40 不平衡分離的分子標記和與種子休眠數量性狀基因座連鎖的分子標記的關係 41 綜合討論 42 第四節 水稻種子休眠數量性狀基因座定位 42 第六章 結論及未來展望 45 第一節 結論 45 第二節 未來展望 45 Selective Genotyping 45 水稻種子休眠數量性狀基因座定位 46 引用文獻 48 圖 54 表 67 附件一 Muranty and Goffinet (1997) 的回交族群的單一標記分析的概度函數推導及修正 86 附件二 F2族群的單一標記分析的概度函數推導 87 附件三 高雄改良場族群基因型鑑定及卡方適合度檢定結果 88 附件四 花蓮改良場族群基因型鑑定及卡方適合度檢定結果 93 | |
| dc.language.iso | zh-TW | |
| dc.subject | selective genotyping | zh_TW |
| dc.subject | 種子休眠性 | zh_TW |
| dc.subject | 穗上發芽 | zh_TW |
| dc.subject | 數量性狀基因座定位 | zh_TW |
| dc.subject | quantitative trait locus mapping | en |
| dc.subject | selective genotyping | en |
| dc.subject | pre-harvest sprouting | en |
| dc.subject | seed dormancy | en |
| dc.title | 使用Selective Genotyping鑑定已知的水稻種子休眠與穗上發芽數量性狀基因座 | zh_TW |
| dc.title | Identification of Known Rice QTLs for Seed Dormancy and Pre-harvest Sprouting Using Selective Genotyping | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 胡凱康(Kae-Kang Hwu),高振宏(Chen-Hung Kao) | |
| dc.subject.keyword | 種子休眠性,穗上發芽,數量性狀基因座定位,selective genotyping, | zh_TW |
| dc.subject.keyword | seed dormancy,pre-harvest sprouting,quantitative trait locus mapping,selective genotyping, | en |
| dc.relation.page | 95 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-08-28 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| 顯示於系所單位： | 農藝學系 | |
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