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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 沈弘俊 | |
| dc.contributor.author | Zheng-Yu Chen | en |
| dc.contributor.author | 陳正育 | zh_TW |
| dc.date.accessioned | 2021-06-16T16:38:13Z | - |
| dc.date.available | 2018-08-28 | |
| dc.date.copyright | 2012-10-12 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-09-28 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63381 | - |
| dc.description.abstract | 近年來已有許多醫學報告指出血清中C反應蛋白的濃度可以作為心血管疾病危險因子指標，在本研究中，吾人藉由量測血清中螢光抗體粒子球之布朗運動做為檢測機制去偵測血清中Ｃ反應蛋白的濃度，當血清中之Ｃ反應蛋白抗原與螢光粒子表面之抗體反應時，粒子粒徑會變大，而造成其布朗運動速度值逐漸減慢，吾人在本研究中使用全反射螢光顯微技術以及微粒子追蹤測速技術分析得到抗原與抗體粒子球之結合過程中的布朗擴散特徵，在本研究中吾人成功量測十二種抗原濃度之血清，並利用此結果將心血管疾病之低、中、高危險指標區域中做更精細的分級，而由實驗結果可以看出在抗原抗體結合過程中其螢光粒子粒徑變化程度與血清中之抗原濃度相關。在高濃度抗原血清中，因為相同體積下抗原數目較多因而使抗體粒子球粒徑變化較大，進而造成其速度變化趨勢較低濃度抗原血清中之螢光粒子快。
在未來可以使用這機制簡單的檢測技術大量收集不同血清樣本的速度時間曲線資料，並且使每一個速度曲線都相對應血清樣本內某特定抗原濃度，已期待可以實際運用在心血管疾病危險因子之臨床檢測上。 | zh_TW |
| dc.description.abstract | The concentration of C-reactive protein (CRP) in serum is the symptom of risk factor for cardiovascular diseases (CVD). In this research, a new bio-sensing technique was applied to detect the concentration of CRP in serum by measuring the Brownian motion of fluorescent particles whose surface has been coated with anti-CRP. When CRP antigens react with anti-CRP coated on the surface of fluorescent particles, the diameter of particles will increase and the Brownian motion velocity of particle gradually slows down. Both Total Internal Reflection Fluorescence Microscopy (TIRFM) and Micro-Particle-Tracking-Velocimetry (μ-PTV) technique are utilized to obtain the characteristics of particles’ Brownian diffusion during the reaction process. In this study, I totally measure twelve different samples with different CRP concentration, and risk region for cardiovascular disease are successfully divided into twelve regions by different Brownian velocity profiles in different concentrations of CRP.
The experiment results show that the degree of variation in beads’ diameters relates to the concentration of CRP antigen in serum during the binding process. Because there are larger amount of antigen in serum with higher concentration of CRP antigen, it leads to Brownian velocity of the particles in higher antigen concentration change more rapidly than Brownian velocity of the particles in lower antigen concentration. In the future, this simple sensing technique can be used to collect a large of Brownian velocity profile data, and every profile can represent each situation of specific antigen concentration and be applied to the clinical assay of cardiovascular diseases risk factors. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T16:38:13Z (GMT). No. of bitstreams: 1 ntu-101-R99543021-1.pdf: 4840474 bytes, checksum: 0ce4bf5a8d4218bca50d68d6cac3c071 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書 # 誌謝 i 中文摘要 ii ABSTRACT iii 目錄 iv 表目錄 vii 圖目錄 viii 符號目錄 xi 第一章 緒論 1 1-1 前言 1 1-2 研究動機與目的 3 1-3 研究方法 4 1-4 論文架構 5 第二章 文獻回顧 7 2-1 C反應蛋白簡介 7 2-2 粒子影像/追蹤測速儀與全反射螢光顯微技術 9 2-3 布朗運動 11 第三章 實驗理論與技術背景 13 3-1 布朗運動 13 3-1.1 前言 13 3-1.2 愛因斯坦布朗運動數學模型 14 3-1.3 朗之文布朗運動數學模型 20 3-1.4 膠體粒子的布朗擴散與重力沉降平衡模型 22 3-1.5 布朗運動的邊界效應 23 3-2 全反射螢光顯微技術 26 3-2.1 前言 26 3-2.2 漸逝波原理介紹 26 3-2.3 螢光分子非等向性放射 28 3-2.4 漸逝波偏振態 28 3-2.5 中間層分析 30 3-3 粒子影像/追蹤測速技術工作原理 31 第四章 實驗設備架構與實驗步驟 33 4-1 微粒子影像/追蹤測速實驗設備 33 4-1.1 光源裝置 33 4-1.2 影像擷取裝置 34 4-1.3 光學顯微鏡 34 4-1.4 同步裝置 35 4-1.5 粒子影像分析軟體 35 4-1.6 光學路徑架構 35 4-2 抗體自組裝與螢光粒子表面實驗 36 4-2.1 螢光粒子的選用 36 4-2.2 抗體自組裝與螢光粒子表面實驗 37 4-3 咖啡環效應與觀測腔體製作 40 4-3.1 PDMS圓形腔體製作 40 4-3.2 雙玻片夾層腔體製 41 4-4 粒徑變化驗證實驗 42 4-5 全反射螢光顯微技術之漸逝波照明深度量測實驗 42 4-6 Ｃ反應蛋白抗原抗體辨識實驗 43 4-6.1 Ｃ反應蛋白抗原抗體辨識實驗 43 4-6.2 血清黏度穩定性 44 4-6.3 血清自體性螢光量測 44 第五章 實驗結果與討論 45 5-1 μ-PTV於奈米粒子之布朗運動分析 45 5-1.1 螢光抗體球濃度參數之決定 46 5-1.2 漸逝波照明深度參數之決定 47 5-1.3 電荷耦合元件視野校正實驗與檢視網格尺寸參數決定 48 5-1.4 觀測系統上其它限制 49 5-2 粒徑變化驗證實驗 49 5-3 血清黏度穩定性量測分析 50 5-4 血清自體性螢光量測分析 51 5-5 Ｃ反應蛋白抗原抗體辨識實驗分析 51 第六章 研究結論與未來展望 57 6-1 研究結論 57 6-2 未來展望 58 參考文獻 60 附錄一(數據分析處理程式) 102 附錄二(CHC碼之意義) 106 附錄三(數據有效值與粒子數目對之關係) 107 | |
| dc.language.iso | zh-TW | |
| dc.subject | 全反射螢光顯微技術 | zh_TW |
| dc.subject | C反應蛋白 | zh_TW |
| dc.subject | 微粒子追蹤測速儀 | zh_TW |
| dc.subject | 布朗運動 | zh_TW |
| dc.subject | Brownian motion | en |
| dc.subject | micro Particle Tracking Velocimetry (μ-PTV) | en |
| dc.subject | C-reactive protein | en |
| dc.subject | Total Internal Reflection Fluorescence Microscopy(TIRFM) | en |
| dc.title | 應用粒子布朗運動檢測技術結合漸逝波照明於血清中量測C反應蛋白濃度 | zh_TW |
| dc.title | Measurement of Nano-particle's Brownian Motion for Detecting Concentration of CRP in serum with Evanescent Wave Illumination | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳光鐘,張正憲 | |
| dc.subject.keyword | 布朗運動,微粒子追蹤測速儀,C反應蛋白,全反射螢光顯微技術, | zh_TW |
| dc.subject.keyword | Brownian motion,micro Particle Tracking Velocimetry (μ-PTV),C-reactive protein,Total Internal Reflection Fluorescence Microscopy(TIRFM), | en |
| dc.relation.page | 107 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-09-28 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 應用力學研究所 | zh_TW |
| 顯示於系所單位： | 應用力學研究所 | |
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