請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63181完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 戴榮湘 | |
| dc.contributor.author | Hong-Ming Hsu | en |
| dc.contributor.author | 許弘明 | zh_TW |
| dc.date.accessioned | 2021-06-16T16:26:45Z | - |
| dc.date.available | 2018-03-04 | |
| dc.date.copyright | 2013-03-04 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-01-18 | |
| dc.identifier.citation | 1.Alderete JF. 1999. Iron modulates phenotypic variation and phosphorylation of P270 in double-stranded RNA virus-infected Trichomonas vaginalis. Infect. Immun. 67:4298-4302.
2.Alderete JF, Milsap KW, Lehker MW, Benchimol M. 2001. Enzymes on microbial pathogens and Trichomonas vaginalis: molecular mimicry and functional diversity. Cell Microbiol. 3:359-370. 3.Alderete JF, Nguyen J, Mundodi V, Lehker HW. 2004. Heme-iron increases level of AP65-mediated adherence by Trichomonas vaginalis. Microb. Pathog. 36:263-271. 4.Alderete JF, O'Brien JL, Arroyo R, Engbring JA, Musatovova O, Lopez O, Lauriano C, Nguyen J. 1995. Cloning and molecular characterization of two genes encoding adhesion proteins involved in Trichomonas vaginalis cytoadherence. Mol. Microbiol. 17:69-83. 5.Alderete JF, Provenzano D, Lehker HW. 1995. Iron mediates Trichomonas vaginalis resistance to complement lysis. Microb. Pathog. 19:93-103. 6.Andersson KB, Kowenz-Leutz E, Brendeford EM, Tygsett AH, Leutz A, Gabrielsen OS. 2003. Phosphorylation- dependent down-regulation of c-Myb DNA binding is abrogated by a point mutation in the v-myb oncogene. J Biol Chem. 278: 3816-3824. 7.Arroyo R, Engbring J, Nguyen J, Musatovova O, López O, Lauriano C, Alderete JF. 1995. Characterization of cDNAs encoding adhesin proteins involved in trichomonas vaginalis cytoadherence. Arch Med Res. 26:361-9. 8.Arroyo R, Engbring J, Alderete JF. 1992. Molecular basis of host epithelial cell recognition by Trichomonas vaginalis. Mol Microbiol. 6:853-862. 9.Bastida-Corcuera FD, Okumura CY, Colocoussi A, Johnson PJ. 2005. Trichomonas vaginalis lipophosphoglycan mutants have reduced adherence and cytotoxicity to human ectocervical cells. Eukaryot. Cell 4:1951-1958. 10.Bies J, Markus J, Wolff L. 2002. Covalent attachment of the SUMO-1 protein to the negative regulatory domain of the c-Myb transcription factor modifies its stability and transactivation capacity. J Biol Chem 277:8999-9009. 11.Bies J, Feiková S, Markus J, Wolff L. 2001. Phosphorylation-dependent conformation and proteolytic stability of c-Myb. Blood Cells Mol Dis. 27:422-428. 12.Bogerd HP, Fridell RA, Benson RE, Hua J, Cullen BR. 1996. Protein sequence requirements for function of the human T-cell leukemia virus type 1 Rex nuclear export signal delineated by a novel in vivo randomization-selection assay. Mol. Cell. Biol. 16: 4207–4214. 13.Boulikas T. 1993. Nuclear localization signals （NLS）. Crit. Rev. Eukaryot. Gene Expr. 3:193–227. 14.Boulikas T. 1994. Putative nuclear localization signals （NLS） in protein transcription factors. J. Cell. Biochem. 55:32-58. 15.Brugerolle G, Bricheux G, and Coffe G. 2000. Immunolocalization of two hydrogenosomal enzymes of Trichomonas vaginalis. Parasitol. Res. 86:30–35. 16.Carlton JM, Hirt RP, Silva JC, Delcher AL, Schatz M, Zhao Q, Wortman JR, Bidwell SL, Alsmark UC, Besteiro S, Sicheritz-Ponten T, Noel CJ, Dacks JB, Foster PG, Simillion C, Van de Peer Y, Miranda-Saavedra D, Barton GJ, Westrop GD, Müller S, Dessi D, Fiori PL, Ren Q, Paulsen I, Zhang H, Bastida-Corcuera FD, Simoes-Barbosa A, Brown MT, Hayes RD, Mukherjee M, Okumura CY, Schneider R, Smith AJ, Vanacova S, Villalvazo M, Haas BJ, Pertea M, Feldblyum TV, Utterback TR, Shu CL, Osoegawa K, de Jong PJ, Hrdy I, Horvathova L, Zubacova Z, Dolezal P, Malik SB, Logsdon JM Jr, Henze K, Gupta A, Wang CC, Dunne RL, Upcroft JA, Upcroft P, White O, Salzberg SL, Tang P, Chiu CH, Lee YS, Embley TM, Coombs GH, Mottram JC, Tachezy J, Fraser-Liggett CM, Johnson PJ. 2007. Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis. Science 315:207–212. 17.Cartwright P, Helin K. 2000. Nucleocytoplasmic shuttling of transcription factors. Cell. Mol. Life. Sci. 57:1193-1206. 18.Chu CH, Chang LC, Hsu HM, Wei SY, Liu HW, Lee Y, Kuo CC, Indra D, Chen C, Ong SJ, Tai JH. 2011. A highly organized structure mediating nuclear localization of a Myb2 transcription factor in the protozoan parasite Trichomonas vaginalis. Eukaryot. Cell 10:1607-1617. 19.Cohen MS, Britigan BE, French M, Bean K. 1987. Preliminary observations on lactoferrin secretion in human vaginal mucus: variation during the menstrual cycle, evidence of hormonal regulation, and implications for infection with Neisseria gonorrhoeae. Am J Obstet Gynecol. 157:1122-1125. 20.Cotch MF, Pastorek JG 2nd, Nugent RP, Hillier SL, Gibbs RS, Martin DH, Eschenbach DA, Edelman R, Carey JC, Regan JA, Krohn MA, Klebanoff MA, Rao AV, Rhoads GG. 1997. Epidemiology and outcomes associated with moderate to heavy Candida colonization during pregnancy. Vaginal Infections and Prematurity Study Group. Am J Obstet Gynecol. 178: 374-380. 21.Dang CV, Lee WM. 1989. Nuclear and nucleolar targeting sequences of c-erb-A, c-myb, N-myc, p53, HSP70, and HIV-tat proteins. J. Biol. Chem. 264:18019-18023. 22.De Freitas J, Wintz H, Kim JH, Poynton H, Fox T, Vulpe C. 2003 Yeast, a model organism for iron and copper metabolism studies. Biometals. 16: 185-197. 23.De Silva DM, Askwith CC, Kaplan J. 1996. Molecular mechanisms of iron uptake in eukaryotes. Physiol Rev. 76: 31-47. 24.Dolezal P, Vanacova S, Tachezy J, Hrdy I. 2004. Malic enzymes of Trichomonas vaginalis: two enzyme families, two distinct origins. Gene 329:81–92. 25.Fichorova RN. 2009. Impact of T. vaginalis infection on innate immune responses and reproductive outcome. J. Reprod. Immunol. 83:185–189. 26.Fornerod M, Ohno M, Yoshida M, Mattaj IW. 1997. CRM1 is an export receptor for leucine-rich nuclear export signals. Cell 90:1051-1060. 27.Ganchi PA, Sun SC, Greene WC, Ballard DW. 1992. IkB/MAD-3 masks the nuclear localization signal of NF-kB p65 and requires the transactivation domain to inhibit NF-kB p65 DNA binding. Mol. Cell. Bioi. 3:1339-1352 28.Ganter B, Chao ST, Lipsick JS. 1999. Transcriptional activation by the Myb proteins requires a specific local promoter structure. FEBS Lett. 460:401–410. 29.Garcia AF, Chang TH, Benchimol M, Klumpp DJ, Lehker MW, Alderete JF. 2003. Iron and contact with cells induce expression of adhesions on surface of Trichomonas vaginalis. Mol. Microbiol. 47:1207-1224. 30.Harlow E, Land D. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 31.Henkel T, Machleidt T, Alkalay I, Krönke M, Ben-Neriah Y, Baeuerle PA. 1993. Rapid proteolysis of IkB-a is necessary for activation of transcription factor NFkB. Nature 365: 182-185. 32.Hochheimer A., Tjian R. 2003. Diversified transcription initiation complexes expand promoter selectivity and tissue-specific gene expression. Genes Dev. 17:1309–1320. 33.Hrdy′ I, and Muller M. 1995. Primary structure of the hydrigenosomal malic enzyme of Trichomonas vaginalis and its relationship to homologous enzymes. J. Eukaryot. Microbiol. 42:593–603. 34.Hsu HM, Ong SJ, Lee MC, Tai JH. 2009. Transcriptional regulation of an iron-inducible gene by differential and alternate promoter entries of multiple Myb proteins in the protozoan parasite Trichomonas vaginalis. Eukaryot. Cell 8:362-372. 35.Hsu HM, Lee Y, Indra D, Wei SY, Liu HW, Chang LC, Chen C, Ong SJ, Tai JH.2012. Iron-Inducible Nuclear Translocation of a Myb3 Transcription Factor in the Protozoan Parasite Trichomonas vaginalis. Eukaryot Cell. 11:1441-50. 36.Huang YC, Su LH, Lee GA, Chiu PW, Cho CC, Wu JY, Sun CH. 2008. Regulation of cyst wall protein promoters by Myb2 in Giardia lamblia. J. Biol. Chem. 283:31021-31029. 37.Humbert-Lan G, Pieler T. 1999. Regulation of DNA-binding activity and nuclear transport of B-Myb in Xenopus oocyte. J. Biol. Chem. 274:10293–10300. 38.Jans DA. 1995. The regulaion of protein transport to the nucleus by phosphorylation. Biochem. J. 311:705-716. 39.Jiang I, Tsai CK, Chen SC, Wang SH, Amiraslanov I, Chang CF, Wu WJ, Tai JH, Liaw YC, Huang TH. 2011. Molecular basis of the recognition of the ap65-1 gene transcription promoter elements by a Myb protein from the protozoan parasite Trichomonas vaginalis. Nucleic Acids Res. 39:8992-9008 40.Kang Y, Cullen BR. 1999. The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences. Genes Dev. 13:1126-1139. 41.Kim YS, Song HO, Choi IH, Park SJ, Ryu JS. 2006. Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions. Korean J Parasitol. 44:373-8. 42.Kudo N, Wolff B, Sekimoto T, Schreiner EP, Yoneda Y, Yanagida M, Horinouchi S, Yoshida M. 1998. Leptomycin B Inhibition of Signal-Mediated Nuclear Export by Direct Binding to CRM1. Exp. Cell. Res. 242:540-547. 43.Lane S, Farlie P, Watson R. 1997. B-Myb function can be markedly enhanced by cyclin A-dependent kinase and protein truncation. Oncogene. 14: 2445-2453. 44.Lange A, Mils RL, Lange CJ, Stewart M, Dovine SE, Corbett AH. 2007. Classical nuclear localization signals: Definition, Function, and Interaction with Importin a. J. Biol. Chem. 282:5101-5105. 45.Lei W, Rushton JJ, Davis LM, Liu F, and Ness SA. 2004. Positive and negative determinants of target gene specificity in Myb transcription factors. J. Biol. Chem. 279:29519–29527. 46.Liston DR, and Johnson PJ. 1999. Analysis of a ubiquitous promoter element in a primitive eukaryote: early evolution of the initiator element. Mol. Cell. Biol. 19: 2380–2388. 47.Liston DR, Lau AO, Ortiz D, Smale ST, and Johnson PJ. 2001. Initiator recognition in a primitive eukaryote: IBP39, an initiator-binding protein from Trichomonas vaginalis. Mol. Cell. Biol. 21:7872–7882. 48.Liston, DR, Carrero JC, and Johnson PJ. 1999. Upstream regulatory sequences required for expression of the Trichomonas vaginalis alpha-succinyl CoA synthetase gene. Mol. Biochem. Parasitol. 104:323–329. 49.Liu Y, Kung C, Fishburn J, Ansari AZ, Shokat KM, and Hahn S. 2004. Two cyclin-dependent kinases promote RNA polymerase II transcription and formation of the scaffold complex. Mol. Cell. Biol. 24:1721–1735. 50.Lou YC, Wei SY, Rajasekaran M, Chou CC, Hsu HM, Tai JH, Chen C. 2009. NMR structural analysis of DNA recognition by a novel Myb1 DNA-binding domain in the protozoan parasite Trichomonas vaginalis. Nucleic Acids Res. 37:2381–2394. 51.Makkerh JP, Dingwall C, Laskey RA. 1996. Comparative mutagenesis of nuclear localization signals reveals the importance of neutral and acidic amino acids. Curr Biol. 6:1025-7. 52.Mayo LD, Seo YR, Jackson MW, Smith ML, Guzman JR, Korgaonkar CK, Donner DB. 2005. Phosphorylation of human p53 at serine 46 determines promoter selection and whether apoptosis is attenuated or amplified. J. Biol. Chem. 280:25953–25959. 53.Miglarese MR, Richardson AF, Aziz N, Bender TP. 1996. Differential regulation of c-Myb-induced transcription activation by a phosphorylation site in the negative regulatory domain. J Biol Chem. 271:22697-22705. 54.Moreno-Brito V, Yáñez-Gómez C, Meza-Cervantez P, Avila-González L, Rodríguez MA, Ortega-López J, González-Robles A, Arroyo R. 2005. A Trichomonas vaginalis 120 kDa protein with identity to hydrogenosome pyruvate:ferredoxin oxidoreductase is a surface adhesin induced by iron. Cell. Microbiol. 7:245-258. 55.Müller F, Tora L. 2004. The multicoloured world of promoter recognition complexes. EMBO J. 23:2–8. 56.Müller M. 1993. The hydrogenosome. J Gen Microbiol. 12:2879-89. 57.Mundodi V, Kucknoor AS, Klumpp DJ, Chang TH, Alderete JF. 2004. Silencing the ap65 gene reduces adherence to vaginal epithelial cells by Trichomonas vaginalis. Mol. Microbiol. 53:1099-1108. 58.Nanda N, Michel RG, Kurdgelashvili G, Wendel KA. 2006. Trichomoniasis and its treatment. Expert Rev Anti Infect Ther. 4:125-135. 59.Ness SA. 2003. Myb protein specificity: evidence of a context-specific transcription factor code. Blood Cells Mol. Dis. 31:192–200. 60.O'Brien JL, Lauriano CM, Alderete JF. 1996. Molecular characterization of a third malic enzyme-like AP65 adhesin gene of Trichomonas vaginalis. Microb Pathog. 20:335-349. 61.Ogata K, Morikawa S, Nakamura H, Sekikawa A, Inoue T, Kanai H, Sarai A, Ishii S, Nishimura Y. 1994. Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices. Cell 79:639-648. 62.Oh IH, Reddy EP. 1999. The myb gene family in cell growth, differentiation and apoptosis. Oncogene. 18:3017-3033. 63.Ong SJ, Huang SC, Liu HW, Tai JH. 2004. Involvement of multiple DNA elements in iron-inducible transcription of the ap65-1 genfe in the protozoan parasite Trichomonas vaginalis. Mol. Microbiol. 52:1721-1730. 64.Ong SJ, Hsu HM, Liu HW, Chu CH, Tai JH. 2006. Multifarious transcriptional regulation of adhesion protein gene ap65-1 by a novel Myb1 protein in the protozoan parasite Trichomonas vaginalis. Eukaryot. Cell 5:391-399. 65.Ong SJ, Hsu HM, Liu HW, Chu CH, Tai JH. 2007. Activation of multifarious transcription of an adhesion protein ap65-1 gene by a novel Myb2 protein in the protozoan parasite Trichomonas vaginalis. J. Biol. Chem. 282:6716–6725. 66.Papanikolaou G, Pantopoulos K. 2005. Iron metabolism and toxicity. Toxicol Appl Pharmacol. 202:199-211. 67.Reich NC, Liu L. 2006. Tracking STAT nuclear traffic. Nat Rev Immunol. 6:602-612. 68.Rouault TA. 2006. The role of iron regulatory proteins in mammalian iron homeostasis and disease. Nat Chem Biol. 2:406-14. 69.Rushton JJ, Davis LM, Lei W, Mo X, Leutz A, Ness SA. 2003. Distinct changes in gene expression induced by A-Myb, B-Myb and c-Myb proteins. Oncogene 22:308–313. 70.Schmierer B, Hill CS. 2007. TGFb–SMAD signal transduction: molecular specificity and functional flexibility. Nat. Rev. Mol. Cell Biol. 8:970-982. 71.Schumacher MA, Lau AO, and Johnson PJ. 2003. Structural basis of core promoter recognition in a primitive eukaryote. Cell 115:413– 424. 72.Shafir SC, Sorvillo FJ, Smith L. 2009. Current issues and considerations regarding trichomoniasis and human immunodeficiency virus in African-Americans, Clin. Microbiol. Rev. 22:37–45. 73.Smale ST, and Kadonaga JT. 2003. The RNA polymerase II core promoter. Annu. Rev. Biochem. 72:449–479. 74.Smith AJ, Chudnovsky L, Simoes-Barbosa A, Delgadillo-Correa MG, Jonsson ZO, Wohlschlegel JA, Johnson PJ. 2011. Novel core promoter elements and a cognate transcription factor in the divergent unicellular eukaryote Trichomonas vaginalis. Mol Cell Biol. 31:1444-1458 75.Sorvillo F, Smith L, Kerndt P, and Ash L. 2001. Trichomonas vaginalis, HIV, and African-Americans. Emerg. Infect. Dis. 7:927–932. 76.Stade K, Ford CS, Guthrie C, Weis K. 1997. Exportin 1 （Crm1p） is an essential nuclear export factor. Cell 90:1041–1050. 77.Stoffler D, Schwarz-Herion K, Aebi U, Fahrenkrog B. 2006. Getting across the nuclear pore complex: new insights into the nuclear cytoplasmic import. Can J. Physiol. Pharmacol. 84:499-507. 78.Stracke R, Werber M, Weisshaar B. 2001. The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol. 4: 447-456 79.Sutcliffe S, Giovannucci E, Gaydos CA, Viscidi RP, Jenkins FJ, Zenilman JM, Jacobson LP, De Marzo AM, Willett WC, Platz EA. 2007. Plasma antibodies against Chlamydia trachomatis, human papillomavirus, and human herpesvirus type 8 in relation to prostate cancer: a prospective study. Cancer Epidemiol Biomarkers Prev. 16:1573-1580. 80.Takemoto Y, Tashiro S, Handa H, Ishii S. 1994. Multiple nuclear localization signals of the B-Myb gene products. FEBS Lett. 350:55–60. 81.Tomita A, Towatari M, Tsuzuki S, Hayakawa F, Kosug H , Tamai K, Miyazaki T, Kinoshita T., Saito H. 2000. c-Myb acetylation at the carboxyl-terminal conserved domain by transcriptional co-activator p300. Oncogene. 19: 444-451. 82.Torres-Romero JC, Arroyo R. 2009. Responsiveness of Trichomonas vaginalis to iron concentrations: evidence for a post-transcriptional iron regulation by an IRE/IRP-like system. Infect Genet Evol. 9:1065-1074. 83.Tsai CD, Liu HW, Tai JH. 2002. Characterization of an iron-responsive promoter in the protozoan pathogen Trichomonas vaginalis. J. Biol. Chem. 277:5153-5162. 84.Tsugawa H, Suzuki H, Matsuzaki J, Hirata K, Hibi T. 2012. FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach. Free Radic Biol Med. 52:1003-1010. 85.Van Ho A, Ward DM, Kaplan J. 2002. Transition metal transport in yeast. Annu Rev Microbiol. 56:237-261 86.Vanacova S, Liston DR, Tachezy J, Johnson PJ.（2003）Molecular biology of the amitochondriate parasites, Giardia intestinalis, Entamoeba histolytica and Trichomonas vaginalis. Int J Parasitol. 33: 235-255. 87.Vanácová S, Rasoloson D, Rázga J, Hrdý I, Kulda J, Tachezy J. 2001. Iron-induced changes in pyruvate metabolism of Tritrichomonas foetus and involvement of iron in expression of hydrogenosomal proteins. Microbiology. 147:53-62. 88.Vanácová S, Yan W, Carlton JM, and Johnson PJ. 2005. Spliceosomal introns in the deep-branching eukaryote Trichomonas vaginalis. Proc. Natl. Acad. Sci. USA 102:4430–4435. 89.Vandromme M, Gauthier-Rouvière C, Lamb N, Fernandez A. 1996. Regulation of transcription factor localization: fine-tuning of gene expression. Trends Biochem. Sci. 21:59-64. 90.Viscogliosi E, Philippe H, Baroin A, Perasso R, Brugerolle G. 1993. Phylogeny of trichomonads based on partial sequences of large subunit rRNA and on cladistic analysis of morphological data. J. Eukaryot. Microbiol. 40:411-21. 91.Walker MD. 1996. Screening expression cDNA libraries, p. 63–66. In K. Docherty （ed.）, Gene transcription: DNA binding proteins. Wiley Interscience, New York, NY. 92.Wei SY, Lou YC, Tsai JY, Ho MR, Chou CC, Rajasekaran M, Hsu HM, Tai JH, Hsiao CD, Chen C. 2012. Structure of the Trichomonas vaginalis Myb3 DNA-binding domain bound to a promoter sequence reveals a unique C-terminal b-hairpin conformation. Nucleic Acids Res. 40:449-60. 93.Weinstock H, Berman S, Cates W Jr. 2000. Sexually transmitted diseases among American youth: incidence and prevalence estimates. Perspect. Sex. Reprod. Health. 36:6-10. 94.Wösten MM, Kox LF, Chamnongpol S, Soncini FC, Groisman EA. 2000 A signal transduction system that responds to extracellular iron. Cell. 103:113-125. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63181 | - |
| dc.description.abstract | 陰道滴蟲寄生於人類泌尿生殖係統內，其能量代謝以及致病因子的轉錄表現受宿主環境中的鐵離子調控。此蟲內有400多個含保守性DNA結合區，R2R3，之Myb型轉錄因子。其中，黏附蛋白質ap65-1基因的轉錄活性受鐵誘導，經由Myb1與Myb2 ， 共同調控 。此二轉錄因子以競合方式進入 ap65-1啟動子上Myb1及Myb2共結合之重疊序列，MRE-1/MRE-2r（TAACGATA），與MRE-2f（TAACGA）。本研究進一步確認，Myb3結合於啟動子MRE-1序列（TAACGA），調控ap65-1基因的基礎與受鐵誘導轉錄活性。經實驗觀察，Myb2及Myb3以競結方式進入ap65-1基因啟動子，而其結合趨勢受鐵離子調控。而Myb1之過度表現，同時抑制Myb2與Myb3進入啟動子能力。綜合以上結果推測，陰道滴蟲以三個Myb轉錄因子進入啟動子時機上的差異，來調控ap65-1基因的轉錄活性。這些Myb轉錄因子的R2R3區域包含約100個氨基酸，由六個螺旋序列組成次級結構。去鐵環境下，Myb3少量存在於細胞核，而鐵刺激導致Myb3快速入核：而Myb2入核不受鐵影響。Myb2之核定位訊號亦涵蓋大部份之R2R3區域。Myb3亦利用類似訊號進行入核調控，但仍需要一段富彈性的C端區域，其中包含三個緊鄰的序列因子，分別控制Myb3受鐵刺激後的細胞質滯留、入核與出核。顯示，陰道滴蟲之Myb以R2R3同時控制啟動子序列的選擇及入核時機：唯各自依其內部結構特徵與周邊序列因子， 以控制下游基因適時適量之表現，維持正常生理運作。 | zh_TW |
| dc.description.abstract | Iron is the key host factor that modulates the energy metabolism and virulence expression of the protozoan parasite, Trichomonas vaginalis. It was demonstrated to regulate gene expression via transcription initiation. Iron-inducible transcription of an adhesion protein, ap65-1, gene in the parasite, was previously demonstrated to involve the Myb1 and Myb2 transcription factors, which share a conserved R2R3 DNA-binding domain, but preferentially bind cis-acting elements, MRE-1/MRE-2r (TAACGATA) and MRE-2f (TATCGT), in the promoter region of the ap65-1 gene. In this study, Myb3 protein was demonstrated to specifically bind a DNA element (TAACGA) in MRE-1 and regulate basal and iron-inducible ap65-1 transcription. Competitive promoter entries of Myb2 and Myb3 were observed when iron concentration in growth medium varied, while promoter entries of both proteins were diminished by overexpressing of Myb1. These results suggest transcriptional activity of the ap65-1 promoter is co-regulated by multiple Myb proteins through differential promoter entry.
Nuclear and cytoplasmic shuttling of Myb3 was further examined. Myb3 was enriched more in the cytoplasm than nucleus, especially when cells were depleted of iron. Iron was found to induce a transient nuclear translocation of Myb3, but not Myb2. A nuclear localization signal（NLS） similar to that of Myb2 was mapped to the highly structured R2R3 DNA-binding domain of Myb3 with a flexible C-terminus Contiguous sequence elements that regulate nuclear translocation at multiple cellular steps, including the cytoplasmic retention, nuclear influx and efflux, were identified within the C-terminal tail. These observations suggest that the R2R3 DNA-binding domain may also serves as a common module for the nuclear translocation of various Myb proteins in the parasite, but each of them may possess intrinsic features for nuclear translocation under specific conditions. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T16:26:45Z (GMT). No. of bitstreams: 1 ntu-102-D96445012-1.pdf: 21258913 bytes, checksum: a5cbd26c2921e56bf7632ff468ca904f (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 口試委員會審定書..........................................i
誌謝.....................................................ii 中文摘要................................................iii 英文摘要.................................................iv 第一章 前言..............................................1 一、陰道滴蟲.............................................1 二、鐵離子的生理角色.....................................1 三、鐵離子對陰道滴蟲重要性...............................2 四、陰道滴蟲基因轉錄.....................................3 五、鐵離子與ap65-1基因啟動子.............................4 六、Myb家族蛋白質........................................4 七、蛋白質的核運輸機制...................................6 八、研究目的.............................................7 第二章 材料與方法........................................8 一、陰道滴蟲細胞株與培養.................................8 二、建構質體.............................................8 三、建構6×His-tag rMyb3重組蛋白質表現質體................9 四、大量表現6×His-tag rMyb3重組蛋白質...................10 五、純化6×His-tag rMyb3蛋白質...........................11 六、anti-Myb3抗血清製備.................................11 七、抗體純化 ...........................................11 八、免疫螢光染色........................................12 九、陰道滴蟲整體蛋白質樣本收集..........................12 十、細胞核蛋白與細胞質蛋白萃取..........................12 十一、蛋白質聚丙烯醯胺膠體電泳..........................13 十二、西方轉漬..........................................13 十三、反轉錄定量PCR.....................................14 十四、北方墨點分析......................................14 十五、放射性寡聚核苷酸探針標定..........................14 十六、電泳遲滯分析......................................14 十七、染色體免疫沉澱分析................................15 十八、圓二色光譜分析....................................15 十九、螢光光譜分析......................................15 第三章 結果.............................................16 I、Myb3之生化特性及轉錄調控機制.........................16 一、Myb3蛋白質序列分析..................................16 二、Myb3轉錄因子的表現..................................16 三、Myb3的DNA結合特性...................................17 四、Myb3調控ap65-1基因轉錄活性..........................17 五、Myb3進入啟動子時機..................................19 II、Myb3之入核機制探討..................................20 六、Myb3核定位訊號......................................20 七、Myb3核定位訊號之結構完整性與入核效率................21 八、Myb3入核受鐵誘導....................................22 第四章 討論.............................................24 I、Myb3之生化特性及轉錄調控機制.........................24 一、Myb3基礎特性........................................24 二、Myb3功能分析........................................26 三、Myb3進入啟動子與基因調控............................26 II、Myb3之入核機制探討..................................27 四、Myb3核定位訊號......................................28 五、Myb3受鐵誘導的入核與出核............................28 六、Myb3入核與訊息傳導..................................29 七、結論................................................30 附圖....................................................31 附表....................................................59 參考文獻................................................61 附錄....................................................69 | |
| dc.language.iso | zh-TW | |
| dc.subject | 啟動子結合 | zh_TW |
| dc.subject | ap65-1基因 | zh_TW |
| dc.subject | 鐵離子 | zh_TW |
| dc.subject | 陰道滴蟲 | zh_TW |
| dc.subject | Myb3 | zh_TW |
| dc.subject | 核定位訊號 | zh_TW |
| dc.subject | 入核與出核 | zh_TW |
| dc.subject | nuclear localization signal | en |
| dc.subject | iron | en |
| dc.subject | ap65-1 | en |
| dc.subject | Myb3 | en |
| dc.subject | promoter entry | en |
| dc.subject | Trichomonas vaginalis | en |
| dc.subject | nuclear influx and efflux | en |
| dc.title | 陰道滴蟲中Myb3轉錄因子功能分析 | zh_TW |
| dc.title | Biochemical characterizations of a Myb3 transcription
factor in Trichomonas vaginalis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-1 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 施嘉和,鄧述諄,陳逸然,許邦弘 | |
| dc.subject.keyword | 陰道滴蟲,鐵離子,ap65-1基因,Myb3,啟動子結合,核定位訊號,入核與出核, | zh_TW |
| dc.subject.keyword | Trichomonas vaginalis,iron,ap65-1,Myb3,promoter entry,nuclear localization signal,nuclear influx and efflux, | en |
| dc.relation.page | 79 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-01-18 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-102-1.pdf 未授權公開取用 | 20.76 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。