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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳俊任 | |
| dc.contributor.author | Chun-Hsien Liu | en |
| dc.contributor.author | 劉俊賢 | zh_TW |
| dc.date.accessioned | 2021-06-16T16:11:58Z | - |
| dc.date.available | 2018-03-06 | |
| dc.date.copyright | 2013-03-06 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-02-18 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62837 | - |
| dc.description.abstract | When cells are stimulated by physical or chemical stress, cells may undergo necrosis and release damage-associated molecular patterns (DAMPs) that can induce inflammation. This kind of inflammation, which occurs in the absence of microbial infection, is called sterile inflammation and is characterized by the recruitment of neutrophils to site of tissue damage. Neutrophils can phagocytose and clear the injured cells; however, the release of reactive oxygen and nitrogen intermediates and granular contents also cause harm to the healthy tissue. The aim of this study is to investigate whether necrotic cells could stimulate neutrophils to form the neutrophil extracellular traps (NETs). Human blood neutrophils treated with necrotic human HL60 cells clearly showed the formation of NETs, which could be visualized by the immunofluorescent staining of extracellular DNA and myeloperoxidase, and quantified by measuring the release of neutrophil DNA after DNase treatment. Necrotic cell-stimulated neutrophils showed increased generation of reactive oxygen species (ROS), which could be responsible for causing NET formation. Necrotic cell-induced NET formation was markedly attenuated when total DNA or protein components in the lysates were depleted by enzyme digestion, suggesting that cellular DNA and proteins could both serve as DAMPs for inducing NET formation. IL-1α has been postulated as a DAMP, and we found that neutrophils could also be induced by recombinant IL-1α to form NETs. In addition, we also observed NET formation when purified mouse bone marrow neutrophils were stimulated with necrotic murine EL4 cells, although it required a longer time of treatment. Taken together, our data demonstrated that neutrophils could be stimulated by necrotic cells to form NETs, indicating that there is a dynamic interplay between the injured cells and the recruited neutrophils during sterile inflammation. Further investigation should be done to elucidate how this interaction may affect tissue homeostasis or pathogenesis. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T16:11:58Z (GMT). No. of bitstreams: 1 ntu-102-R99b22020-1.pdf: 3724068 bytes, checksum: 5269a16999c1c2fc974b046c220cb8f0 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 口試委員審定書 .............................................................................. I
誌謝 ........................................................................................ II Abstract .................................................................................... III 中文摘要 .................................................................................... V Abbreviation ................................................................................ VI 目次 ........................................................................................ VIII 圖表目錄 .................................................................................... XI 前言 ........................................................................................ 1 1. 發炎反應概述 ............................................................................. 1 2. 無菌發炎反應 ............................................................................. 2 3. Damage-associated molecular patterns（DAMPS） ............................................ 3 4. 無菌發炎反應的機制 ....................................................................... 4 5. 嗜中性白血球（neutrophils） .............................................................. 5 6. Neutrophil granules ...................................................................... 5 7. 嗜中性白血球移動至組織的機制 ............................................................. 6 8. 嗜中性白血球毒殺及清除病原體之機制 ....................................................... 7 9. NETs形成之機制 ........................................................................... 8 10. 實驗動機與目標 .......................................................................... 10 材料與方法 .................................................................................. 11 一、實驗材料 ................................................................................ 11 1. 實驗動物 ................................................................................. 11 2. 細胞株 ................................................................................... 11 3. 實驗試劑 ................................................................................. 11 二、實驗方法 ................................................................................ 12 1. 小鼠多形核白血球分離 ..................................................................... 12 2. 人類多形核白血球分離 ..................................................................... 12 3. 製作HistoGrip™ coated coverslips ......................................................... 12 4. 製備壞死細胞 ............................................................................. 13 5. 以螢光顯微鏡觀察嗜中性白血球包外陷阱 ..................................................... 13 6. 偵測ROS生成 .............................................................................. 14 7. 偵測嗜中性白血球釋放之DNA ................................................................ 14 8. 流式細胞儀分析嗜中性白血球純度 ........................................................... 15 9. 細胞激素測定 ............................................................................. 15 10. 統計分析 ................................................................................ 16 實驗結果 .................................................................................... 17 1. 壞死細胞引起小鼠嗜中性白血球形成NETs ..................................................... 17 2. 壞死細胞引起人類嗜中性白血球形成NETs ..................................................... 18 3. 壞死細胞釋放之DNA及蛋白質均對NETs形成相當重要 ............................................ 19 4. IL-1α可促使人類嗜中性白血球產生NETs ..................................................... 20 5. 不同細胞株所含之IL-1α表現量不同.......................................................... 21 結果討論 .................................................................................... 22 結論 ........................................................................................ 26 圖表 ........................................................................................ 27 參考文獻 .................................................................................... 50 | |
| dc.language.iso | zh-TW | |
| dc.subject | 無菌發炎反應 | zh_TW |
| dc.subject | DAMPs | zh_TW |
| dc.subject | 介白素-1α | zh_TW |
| dc.subject | 嗜中性白血球胞外陷阱 | zh_TW |
| dc.subject | NETs | en |
| dc.subject | sterile inflammation | en |
| dc.subject | DAMPs | en |
| dc.subject | IL-1α | en |
| dc.title | 壞死細胞引發之嗜中性白血球胞外陷阱 | zh_TW |
| dc.title | Necrotic cell induced neutrophil extracellular trap | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 徐立中,朱清良 | |
| dc.subject.keyword | 無菌發炎反應,DAMPs,介白素-1α,嗜中性白血球胞外陷阱, | zh_TW |
| dc.subject.keyword | sterile inflammation,DAMPs,NETs,IL-1α, | en |
| dc.relation.page | 56 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-02-18 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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