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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王汎熒 | |
dc.contributor.author | He-Shiuan Liang | en |
dc.contributor.author | 梁赫烜 | zh_TW |
dc.date.accessioned | 2021-06-16T16:09:09Z | - |
dc.date.available | 2018-06-21 | |
dc.date.copyright | 2013-06-21 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-04-22 | |
dc.identifier.citation | 1 Adams JE, 3rd, Schechtman KB, Landt Y, Ladenson JH, Jaffe AS: Comparable detection of acute myocardial infarction by creatine kinase MB isoenzyme and cardiac troponin I. Clin Chem 40: 1291-1295, 1994
2 Apple FS: Predictive Value of Cardiac Troponin I and T for Subsequent Death in End-Stage Renal Disease. Circulation 106: 2941-2945, 2002 3 Bishop SP: Necropsy techniques for the heart and great vessels. In: Textbook of canine and feline cardiology: principles and clinical practice, eds. Fox PR, Sisson DMoïse NS, 2nd ed. Saunders, Philadelphia, PA, 1999 4 Chan D, Ng LL: Biomarkers in acute myocardial infarction. BMC Medicine 8: 34, 2010 5 Denzel MS, Scimia MC, Zumstein PM, Walsh K, Ruiz-Lozano P, Ranscht B: T-cadherin is critical for adiponectin-mediated cardioprotection in mice. J Clin Invest 120: 4342-4352, 2010 6 Doran JP, Howie AJ, Townend JN, Bonser RS: Detection of myocardial infarction by immunohistological staining for C9 on formalin fixed, paraffin wax embedded sections. Journal of Clinical Pathology 49: 34-37, 1996 7 Edston E, Kawa K: Immunohistochemical detection of early myocardial infarction. An evaluation of antibodies against the terminal complement complex (C5b-9). International Journal of Legal Medicine 108: 27-30, 1995 8 Emmert-Buck MR, Roth MJ, Zhuang Z, Campo E, Rozhin J, Sloane BF, Liotta LA, Stetler-Stevenson WG: Increased gelatinase A (MMP-2) and cathepsin B activity in invasive tumor regions of human colon cancer samples. Am J Pathol 145: 1285-1290, 1994 9 Entam ML, Kanike K, Goldstein MA, Nelson TE, Bornet EP, Futch TW, Schwartz A: Association of gylcogenolysis with cardiac sarcoplasmic reticulum. J Biol Chem 251: 3140-3146, 1976 10 Entman ML, Bornet EP, Van Winkle WB, Goldstein MA, Schwartz A: Association of glycogenolysis with cardiac sarcoplasmic recticulum: II. Effect of glycogen depletion, deoxycholate solubilization and cardiac ischemia: Evidence for a phosphorylase kinase membrane complex. Journal of Molecular and Cellular Cardiology 9: 515-528, 1977 11 Fawcett DW, McNutt NS: The ultrastructure of the cat myocardium. I. Ventricular papillary muscle. J Cell Biol 42: 1-45, 1969 12 Fishbein MC, Wang T, Matijasevic M, Hong L, Apple FS: Myocardial tissue troponins T and I. Cardiovascular Pathology 12: 65-71, 2003 13 Gao CQ, Sawicki G, Suarez-Pinzon WL, Csont T, Wozniak M, Ferdinandy P, Schulz R: Matrix metalloproteinase-2 mediates cytokine-induced myocardial contractile dysfunction. Cardiovasc Res 57: 426-433, 2003 14 Glatz JFC, van Bilsen M, Paulussen RJA, Veerkamp JH, van der Vusse GJ, Reneman RS: Release of fatty acid-binding protein from isolated rat heart subjected to ischemia and reperfusion or to the calcium paradox. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 961: 148-152, 1988 15 Glatz JFC, van der Vusse GJ, Simoons ML, Kragten JA, van Dieijen-Visser MP, Hermens WT: Fatty acid-binding protein and the early detection of acute myocardial infarction. Clinica Chimica Acta 272: 87-92, 1998 16 Glatz JFC, Vork MM, van der Vusse GJ: Significance of cytoplasmic fatty acid-binding protein for the ischemic heart. Molecular and Cellular Biochemistry 123: 167-173, 1993 17 Hamm CW: Cardiac Troponin Elevations in Patients Without Acute Coronary Syndrome. Circulation 106: 2871-2872, 2002 18 Hamm CW, Ravkilde J, Gerhardt W, Jorgensen P, Peheim E, Ljungdahl L, Goldmann B, Katus HA: The prognostic value of serum troponin T in unstable angina. N Engl J Med 327: 146-150, 1992 19 Hugo F, Hamdoch T, Mathey D, Schafer H, Bhakdi S: Quantitative measurement of SC5b-9 and C5b-9(m) in infarcted areas of human myocardium. Clin Exp Immunol 81: 132-136, 1990 20 Jaffe AS, Babuin L, Apple FS: Biomarkers in Acute Cardiac DiseaseThe Present and the Future. Journal of the American College of Cardiology 48: 1-11, 2006 21 Jenkins CP, Cardona DM, Bowers JN, Oliai BR, Allan RW, Normann SJ: The utility of C4d, C9, and troponin T immunohistochemistry in acute myocardial infarction. Arch Pathol Lab Med 134: 256-263, 2010 22 Katus HA, Remppis A, Neumann FJ, Scheffold T, Diederich KW, Vinar G, Noe A, Matern G, Kuebler W: Diagnostic efficiency of troponin T measurements in acute myocardial infarction. Circulation 83: 902-912, 1991 23 Katus HA, Remppis A, Scheffold T, Diederich KW, Kuebler W: Intracellular compartmentation of cardiac troponin T and its release kinetics in patients with reperfused and nonreperfused myocardial infarction. The American Journal of Cardiology 67: 1360-1367, 1991 24 Katus HA, Schoeppenthau M, Tanzeem A, Bauer HG, Saggau W, Diederich KW, Hagl S, Kuebler W: Non-invasive assessment of perioperative myocardial cell damage by circulating cardiac troponin T. Br Heart J 65: 259-264, 1991 25 Khaw BA, Gold HK, Fallon JT, Haber E: Detection of serum cardiac myosin light chains in acute experimental myocardial infarction: radioimmunoassay of cardiac myosin light chains. Circulation 58: 1130-1136, 1978 26 Klein HH, Puschmann S, Schaper J, Schaper W: The mechanism of the tetrazolium reaction in identifying experimental myocardial infarction. Virchows Archiv 393: 287-297, 1981 27 Kleine AH, Glatz JFC, Van Nieuwenhoven FA, Van der Vusse GJ: Release of heart fatty acid-binding protein into plasma after acute myocardial infarction in man. Molecular and Cellular Biochemistry 116: 155-162, 1992 28 Koenig SC, Litwak KN, Giridharan GA, Pantalos GM, Dowling RD, Prabhu SD, Slaughter MS, Sobieski MA, Spence PA: Acute Hemodynamic Efficacy of a 32-ml Subcutaneous Counterpulsation Device in a Calf Model of Diminished Cardiac Function. ASAIO Journal 54: 578-584, 2008 29 Kraus B, Cain H: Giant mitochondria in the human myocardium--morphogenesis and fate. Virchows Arch B Cell Pathol Incl Mol Pathol 33: 77-89, 1980 30 Krause E-G, Rabitzsch G, Noll F, Maire J, Puschendorf B: Glycogen phosphorylase isoenzyme BB in diagnosis of myocardial ischaemic injury and infarction. Molecular and Cellular Biochemistry 160-161: 289-295, 1996 31 Litwak KN, McMahan A, Lott KA, Lott LE, Koenig SC: Monensin toxicosis in the domestic bovine calf: a large animal model of cardiac dysfunction. Contemp Top Lab Anim Sci 44: 45-49, 2005 32 Luca M, Huang S, Gershenwald JE, Singh RK, Reich R, Bar-Eli M: Expression of interleukin-8 by human melanoma cells up-regulates MMP-2 activity and increases tumor growth and metastasis. Am J Pathol 151: 1105-1113, 1997 33 Mathey D, Schofer J, Schafer HJ, Hamdoch T, Joachim HC, Ritgen A, Hugo F, Bhakdi S: Early accumulation of the terminal complement-complex in the ischaemic myocardium after reperfusion. Eur Heart J 15: 418-423, 1994 34 Mihatsch K, Nestler M, Saluz HP, Henke A, Munder T: Proapoptotic protein Siva binds to the muscle protein telethonin in cardiomyocytes during coxsackieviral infection. Cardiovascular Research 81: 108-115, 2008 35 Nachlas MM, Shnitka TK: Macroscopic identification of early myocardial infarcts by alterations in dehydrogenase activity. Am J Pathol 42: 379-405, 1963 36 Nicholas G, Thomas M, Langley B, Somers W, Patel K, Kemp CF, Sharma M, Kambadur R: Titin-cap associates with, and regulates secretion of, Myostatin. Journal of Cellular Physiology 193: 120-131, 2002 37 Niwano K, Arai M, Koitabashi N, Watanabe A, Ikeda Y, Miyoshi H, Kurabayashi M: Lentiviral Vector-mediated SERCA2 Gene Transfer Protects Against Heart Failure and Left Ventricular Remodeling After Myocardial Infarction in Rats. Mol Ther 16: 1026-1032, 2008 38 Okamoto F, Sohmiya K, Ohkaru Y, Kawamura K, Asayama K, Kimura H, Nishimura S, Ishii H, Sunahara N, Tanaka T: Human Heart-Type Cytoplasmic Fatty Acid-Binding Protein (H-FABP) for the Diagnosis of Acute Myocardial Infarction. Clinical Evaluation of H-FABP in Comparison with Myoglobin and Creatine Kinase Isoenzyme MB. In: Clinical Chemistry and Laboratory Medicine, p. 231. 2000 39 Peetz D, Post F, Schinzel H, Schweigert R, Schollmayer C, Steinbach K, Dati F, Noll F, Lackner KJ: Glycogen phosphorylase BB in acute coronary syndromes. Clinical Chemical Laboratory Medicine 43: 1351-1358, 2005 40 Pruszczyk P: Cardiac Troponin T Monitoring Identifies High-Risk Group of Normotensive Patients With Acute Pulmonary Embolism. Chest 123: 1947-1952, 2003 41 Puleo PR, Meyer D, Wathen C, Tawa CB, Wheeler S, Hamburg RJ, Ali N, Obermueller SD, Triana JF, Zimmerman JL, et al.: Use of a rapid assay of subforms of creatine kinase-MB to diagnose or rule out acute myocardial infarction. N Engl J Med 331: 561-566, 1994 42 Ramkissoon RA: Macroscopic identification of early myocardial infarction by dehydrogenase alterations. J Clin Pathol 19: 479-481, 1966 43 Ranscht B, Dours-Zimmermann MT: T-cadherin, a novel cadherin cell adhesion molecule in the nervous system lacks the conserved cytoplasmic region. Neuron 7: 391-402, 1991 44 Reddy KS, Yusuf S: Emerging epidemic of cardiovascular disease in developing countries. Circulation 97: 596-601, 1998 45 Reitan O, Steen S, Ohlin H: Left ventricular heart failure model for testing cardiac assist devices. ASAIO J 48: 71-75, 2002 46 Remppis A, Scheffold T, Greten J, Haass M, Greten T, Kübler W, Katus HA: Intracellular compartmentation of troponin T: Release kinetics after global ischemia and calcium paradox in the isolated perfused rat heart. Journal of Molecular and Cellular Cardiology 27: 793-803, 1995 47 Rosalki SB: Cardiac Biomarkers for Detection of Myocardial Infarction: Perspectives from Past to Present. Clinical Chemistry 50: 2205-2213, 2004 48 Sawicki G: Degradation of Myosin Light Chain in Isolated Rat Hearts Subjected to Ischemia-Reperfusion Injury: A New Intracellular Target for Matrix Metalloproteinase-2. Circulation 112: 544-552, 2005 49 Schafer H, Mathey D, Hugo F, Bhakdi S: Deposition of the terminal C5b-9 complement complex in infarcted areas of human myocardium. J Immunol 137: 1945-1949, 1986 50 Smolina K, Wright FL, Rayner M, Goldacre MJ: Incidence and 30-day case fatality for acute myocardial infarction in England in 2010: national-linked database study. The European Journal of Public Health, 2012 51 Sobel BE, Roberts R, Larson KB: Considerations in the use of biochemical markers of ischemic injury. Circ Res 38: I99-108, 1976 52 Spinale FG: Myocardial matrix remodeling and the matrix metalloproteinases: influence on cardiac form and function. Physiol Rev 87: 1285-1342, 2007 53 Stone MJ, Waterman MR, Harimoto D, Murray G, Willson N, Platt MR, Blomqvist G, Willerson JT: Serum myoglobin level as diagnostic test in patients with acute myocardial infarction. Br Heart J 39: 375-380, 1977 54 Sung MM, Schulz CG, Wang W, Sawicki G, Bautista-López NL, Schulz R: Matrix metalloproteinase-2 degrades the cytoskeletal protein α-actinin in peroxynitrite mediated myocardial injury. Journal of Molecular and Cellular Cardiology 43: 429-436, 2007 55 Sylven C, Bendz R: Myoglobin, creatine kinase and its isoenzyme MB in serum after acute myocardial infarction. Eur J Cardiol 8: 515-521, 1978 56 Tavazzi L: Clinical epidemiology of acute myocardial infarction. American Heart Journal 138: S48-S54, 1999 57 Thygesen K, Alpert JS, White HD, Jaffe AS, Apple FS, Galvani M, Katus HA, Newby LK, Ravkilde J, Chaitman B, Clemmensen PM, Dellborg M, Hod H, Porela P, Underwood R, Bax JJ, Beller GA, Bonow R, Van Der Wall EE, Bassand JP, Wijns W, Ferguson TB, Steg PG, Uretsky BF, Williams DO, Armstrong PW, Antman EM, Fox KA, Hamm CW, Ohman EM, Simoons ML, Poole-Wilson PA, Gurfinkel EP, Lopez-Sendon JL, Pais P, Mendis S, Zhu JR, Wallentin LC, Fernandez-Aviles F, Fox KM, Parkhomenko AN, Priori SG, Tendera M, Voipio-Pulkki LM, Vahanian A, Camm AJ, De Caterina R, Dean V, Dickstein K, Filippatos G, Funck-Brentano C, Hellemans I, Kristensen SD, McGregor K, Sechtem U, Silber S, Widimsky P, Zamorano JL, Morais J, Brener S, Harrington R, Morrow D, Lim M, Martinez-Rios MA, Steinhubl S, Levine GN, Gibler WB, Goff D, Tubaro M, Dudek D, Al-Attar N: Universal definition of myocardial infarction: Kristian Thygesen, Joseph S. Alpert and Harvey D. White on behalf of the Joint ESC/ACCF/AHA/WHF Task Force for the Redefinition of Myocardial Infarction. European Heart Journal 28: 2525-2538, 2007 58 Vakeva A, Morgan BP, Tikkanen I, Helin K, Laurila P, Meri S: Time course of complement activation and inhibitor expression after ischemic injury of rat myocardium. Am J Pathol 144: 1357-1368, 1994 59 Valle G, Faulkner G, De Antoni A, Pacchioni B, Pallavicini A, Pandolfo D, Tiso N, Toppo S, Trevisan S, Lanfranchi G: Telethonin, a novel sarcomeric protein of heart and skeletal muscle. FEBS Letters 415: 163-168, 1997 60 van der Veen KJ, Willebrands AF: Isoenzymes of creatine phosphokinase in tissue extracts and in normal and pathological sera. Clin Chim Acta 13: 312-316, 1966 61 Varki AP, Roby DS, Watts H, Zatuchni J: Serum myoglobin in acute myocardial infarction: A clinical study and review of the literature. American Heart Journal 96: 680-688, 1978 62 Wachstein M, Meisel E: Succinic dehydrogenase activity in myocardial infarction and in induced myocardial necrosis. Am J Pathol 31: 353-365, 1955 63 Wang W: Intracellular Action of Matrix Metalloproteinase-2 Accounts for Acute Myocardial Ischemia and Reperfusion Injury. Circulation 106: 1543-1549, 2002 64 Watanabe K, Wakabayashi H, Veerkamp JH, Ono T, Suzuki T: Immunohistochemical distribution of heart-type fatty acid-binding protein immunoreactivity in normal human tissues and in acute myocardial infarct. The Journal of Pathology 170: 59-65, 1993 65 Wilson EM, Spinale FG: Myocardial remodelling and matrix metalloproteinases in heart failure: turmoil within the interstitium. Ann Med 33: 623-634, 2001 66 Zarain-Herzberg A, Afzal N, Elimban V, Dhalla N: Decreased expression of cardiac sarcoplasmic reticulum Ca2+-pump ATPase in congestive heart failure due to myocardial infarction. Molecular and Cellular Biochemistry 163-164: 285-290, 1996 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62743 | - |
dc.description.abstract | 傳統心肌的組織學檢查,在透過蘇木紫(hematoxylin)及伊紅(eosin)的染色後,對於心肌細胞的死亡可以由下列標準(本研究中定為方法A)來判定:1) 核濃縮(pyknosis)、核破裂(karyorrhexis)及核溶解(karyolysis);2) 肌漿斷裂、棉絮化(flocculation)及/或出現收縮帶(contraction band);3) 炎症細胞的浸潤。然而,多數以上變化並無法在心肌細胞死亡後六小時內觀察到。本研究中,使用多種方式實驗性的致使心肌壞死或衰竭,包括:1) 莫能菌素中毒(monensin intoxication);2) 左冠狀動脈前降枝永久性30分鐘結紮(ligation of the left anterior descending (LAD) coronary artery for 30 min without reperfusion); 3) 左冠狀動脈迴旋枝永久性30分鐘結紮(ligation of the left circumflex (LCX) branch of coronary artery for 30 min without reperfusion)及4) 鈣離子阻斷劑併用乙型交感神經拮抗劑之生理性衰竭(application of calcium blocker combined with beta antagonist)。除了上述傳統壞死標準外,本研究中加入了另外三種組織學診斷標準(歸納為方法B),專門針對早期心肌傷害或壞死,包括:1) 彎曲變形或萎縮濃染的細胞核(wavy or condensed nucleus); 2) 過度嗜伊紅性的肌漿伴有細胞腫脹或萎縮(hypereosinophilia of sacroplasm with either swelling or atrophy in diameter); 3) 核旁疑似胞器腫脹(粒線體或肌內質網)混濁腫脹的空泡(perinuclear cloudy vacuolation likely results from swollen organelles such as mitochondria and/or sarcoplasmic reticulum)。 使用免疫組織化學染色針對心肌內結構性蛋白(structural protein)、功能性蛋白(functional protein)、胞器內酵素型蛋白(organelle-binding enzymatic protein)及第九號補體(complement 9)。將上述染色結果依標準量化並以統計比對,結果顯示對於心肌早期壞死及傷害,方法B有著和cardiac troponin I(cTnI)及telethonin (T-cap)相同的有效診斷能力,且強過本實驗中其他早期診斷性標的蛋白(biomarker)。另外,本實驗染色結果中,T-cap 從壞死區的流失現象比現今的黃金標準cTnI略為顯著且廣泛。基質金屬蛋白酶的染色顯示(matrix metalloproteinase 2, MMP2)此酵素不僅同時出現在胞內肌漿及胞外基質,還在部分受損心肌細胞中輕微增加,此耐人尋味的結果意謂著此酵素有潛力做為一心肌壞死標的物。其餘標的物則沒有上述數種蛋白顯著或僅有相似的診斷能力。至於C9在本實驗中則無法提供早期診斷能力(無明顯沉積)。 | zh_TW |
dc.description.abstract | Traditionally histological criteria used to interpret necrotic cardiomyocytes on hematoxylin and eosin (H&E) stained tissue sections (designated as “method A” in this study) are: 1) nuclear pyknosis, karyorrhexis, and karyolysis; 2) sarcoplasmic fragmentation, flocculation, and/or the presence of contraction band; 3) inflammatory infiltration. However, these changes are usually unremarkable until at least 6 hours postnecrotic. In this study, myocardial necrosis were experimentally induced by various models: 1) monensin intoxication; 2) ligation of the left anterior descending (LAD) coronary artery for 30 min without reperfusion; 3) ligation of the left circumflex (LCX) branch of coronary artery for 30 min without reperfusion; and 4) application of calcium blocker combined with beta-antagonist to induce functional left heart failure. Three additional criteria (designated as “method B”) were also proposed in this study to interpret early cardiomyocyte necrosis or myocardial injuries on H&E stained tissue sections. They were: 1) wavy or condensed nucleus; 2) hypereosinophilia of sacroplasm with either swelling or atrophy in diameter; 3) perinuclear cloudy vacuolation likely results from swollen organelles such as mitochondria and/or sarcoplasmic reticulum. Immunohistochemistry (IHC) using antibodies against structural proteins, functional proteins, organelle-binding enzymatic proteins together with complement 9 were also employed. The IHC stained section were scored with a known system and compared statistically with methods A and B for their sensitivity or superiority in detecting early MI in tissue specimens. It revealed that method B was as sensitive as cardiac troponin I (cTnI) and telethonin (T-cap), which were more superior to other biomarkers used in detecting early necrotic events in cardiomyocytes. In instances T-cap was slightly superior to cTnI, the current gold standard. The staining for matrix metalloproteinase 2 (MMP2), an intracellular and extracellular proteins, revealed intriguing results and suggested early activation and early diagnostic potential of this molecule for necrotic events. Other biomarkers were either fair or insensitive (such as deposition C9) for detecting early necrosis in the models used here in this study. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T16:09:09Z (GMT). No. of bitstreams: 1 ntu-102-R99629013-1.pdf: 2248468 bytes, checksum: b33d3c4a58600e14f31cdeab631dcec8 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 中文摘要........................................................................................................................ I
Abstract ........................................................................................................................ III Contents ........................................................................................................................ V List of Tables ............................................................................................................. VIII List of Figures .............................................................................................................. IX List of Appendices ........................................................................................................ X Chapter Ⅰ ..................................................................................................................... 1 INTRODUCTION ................................................................................................. 1 Chapter Ⅱ ..................................................................................................................... 3 LITERATURE REVIEW ............................................................................................... 3 2.1 Heart Disease in Human and Domestic Animals ............................................. 3 2.1.1 Prevalence, Risk factors and Mortality ................................................. 3 2.1.2 Coronary Heart Disease and Myocardial Infarction ............................. 4 2.2 Early Diagnosis of myocardial infarction ........................................................ 6 2.2.1 Structural Proteins in Myocardium ....................................................... 6 2.2.2 Functional Proteins in Myocardium ...................................................... 9 2.2.3 Complement System and Endogenous Proteinases ............................ 13 Chapter Ⅲ ................................................................................................................... 16 AIMS OF STUDY ....................................................................................................... 16 Chapter Ⅳ ................................................................................................................... 17 MATERIALS AND METHODS ................................................................................. 17 4.1 Animals and Heart Failure Models ................................................................ 17 4.2 Animal Tissues and Sampling of the Hearts for histopathology .................... 21 4.3 Antibodies ...................................................................................................... 21 4.4 Immunohistochemistry (IHC) ........................................................................ 22 4.5 Analysis of Results ......................................................................................... 23 Chapter Ⅴ ................................................................................................................... 26 RESULTS ..................................................................................................................... 26 5.1 Histopathological Examination of H&E Stained Myocardial Sections ......... 26 5.1.1 Group of Monensin Intoxication in Bovine ........................................ 26 5.1.2 Group of LAD Ligation in Bovine and Porcine .................................. 26 5.1.3 Group of LCX Ligation in Porcine ..................................................... 27 5.1.4 Group of β-antagonist Combined with Calcium Blocker in Bovine ... 27 5.1.5 Group of Shame Operation in Bovine ................................................ 28 5.1.6 Quantification of Myocardial Lesions in H&E Stained Sections by Method A and Method B .............................................................................. 28 5.2 Examination of IHC Stained Myocardial Tissue Sections ............................. 28 5.2.1 Results of Structural Proteins .............................................................. 28 5.2.2 Results of Functional Proteins ............................................................ 31 5.2.3 Results of Organelle-Binding Enzymatic Proteins ............................. 32 5.2.4 Results of Complement System and Endogenous Proteinases ........... 34 5.2.5 Quantification of scores for 11 Antibodies in IHC Stained Sections .. 35 5.2.6 Statistically Analysis of H&E and IHC Scores ................................... 36 Chapter Ⅵ ................................................................................................................... 39 Discussion and Conclusion .......................................................................................... 39 Tables ........................................................................................................................... 45 Figures.......................................................................................................................... 52 Appendices ................................................................................................................... 64 Appendix 4. 口試紀錄 ................................................................................................ 68 References .................................................................................................................... 71 | |
dc.language.iso | en | |
dc.title | 從動物模型中建立實驗性心肌梗塞之病理診斷準則:
心臟組織之早期損傷鑑定 | zh_TW |
dc.title | Establishment of Pathological Criteria for Experimental
Myocardial Infarction in Animal Model: Assessment of the Early Damage of the Myocardium | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡信雄,許天來,張本恆 | |
dc.subject.keyword | 心衰竭,早期診斷,病理檢查,特殊染色, | zh_TW |
dc.subject.keyword | myocardial infarction,early diagnosis,pathology,IHC, | en |
dc.relation.page | 78 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-04-23 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
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